The biology lab experiments determined the antioxidant activity of ascorbate using ABTS and DPPH assays and measured cell viability in algal cultures using an MTS assay. The ABTS and DPPH assays found that ascorbate inhibits oxidation in a concentration-dependent manner, with higher concentrations showing greater inhibition. The cell viability assay measured increasing cell numbers with higher concentrations of ascorbate. In summary, the experiments showed that ascorbate has antioxidant properties that protect against oxidation and increases cell growth in a concentration-dependent manner.

1. BIOLOGY
LAB
Experiments performed
by:
Humaira Quraishi
Mary Dela Cruz
Hardika Patel

2. Introduction
AO ABTS: Determine whether ascorbate antioxidant
activity does truly protects plasma lipids against damage
by inhibition of metmyoglobin and ABTS oxidation
depending on concentration.
AO DPPH: The purpose of this study was to evaluate the
comparability of antioxidant capacity measurements
obtained by ABTS and DPPH assay, when applied to a
large number of food samples containing a variety of
fruits, vegetables and beverages that are major
contributors to antioxidant intake in the US diet
Cell Viability: The experiment is done to determine cell
viability in algal cultures by using a colorimetric assay
widely used for the estimation of cell proliferation in animal
cells

3. METHOD
1. Antioxidant Activity ABTS
a) Ascorbate inhibits ABTS and metmyoglobin  green color
2. Antioxidant Activity DPPH
a) DPPH radical + ascorbate  removes radical  colorless solution
3. Cell viability
a) Cell count
I. count cells
a) MTS
I. Cells + MTS(yellow)  Formazan(brown crystal)

4. RESULT: ANTIOXIDANT ACTIVITY
ABTS
Application:
Ascorbate is used for
is plasma lipid
protection against
detectable
peroxidative damage
induced by aqueous
radicals.
100 % 110% 122% 36.13% 30.23%
33.68%
0
20
40
60
80
100
120
140
0 0.008 0.04 0.2 1 5
AscorbateABTS
PercentConcentration
Ascorbate (mM)

5. Antioxidant activity DPPH:
Application:
used to monitor
chemical
reactions and
radical
scavenging
activity that
involve radicals
in antioxidant
assay for plant
and food
components
100 77.18 64.05 53.81 48.69
42.22
0
20
40
60
80
100
120
0 0.008 0.04 0.2 1 5
DPPH(%ofContro
Ascorbate (mM)

6. Cell Viability
0.00E+00
5.00E+06
1.00E+07
1.50E+07
2.00E+07
2.50E+07
3.00E+07
3.50E+07
0 1 5
NumberofCellsperdish
Concentration in mM
Cells per dish vs. Concentration
Application: useful
for detecting and
evaluating toxicity
of
chemicals/substanc
es like in cancer
drug analysis or
any health risk
assessments
1.93E+07 2.89E+07 1.23E+07

7. Summary of ABTS and DPPH
Ascorbate (mM) AO ABTS %
Concentration
AO ABTS %
Concentration
CV %
0 100 100 100
0.008 110 77.18 ---
0.04 12 64.05 ---
0.2 36.13 53.81 ---
1 30.23 48.69 150
5 33.68 42.22 42