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Mr. Hemant Singh Kurmi
Assitant Professor
hemantkurmi05@gmail.com
7697614376
Institute of Pharmaceutical Sciences
Basis of classification
• Phenotypic classification
Morphological
Anatomical
Staining
Cultural characteristics
Nutrition
Environmental factors
Biochemical reactions
Antigenic structure
• Genotypic classification
DNA-DNA hybridization
G+C content
2
Morphological classification
S.N Type of Bacteria Shape
1 True Bacteria
I) -Coccus Round
II) -Bacillus Rod
2 Spirillum Small, spiral
3 Spirochete Longer,
slender-spiral
4 Vibro Coma (,)
5 Mycoplasm Irregular
6 Actinomycine Sun Light ray
Bacteria canbe classified into sixmajor groups on morphological basis.
• Cocci – These are spherical or oval cells. On the
basis of arrangement of individual organisms
they can be described as
– Monococci (Cocci in singles)
Ex.- Monococcus spp.
– Diplococci (Cocci in pairs)
Ex.- Streptococcus pneumoniae
– Staphylococci (Cocci in grape-like clusters)
Ex.- Staphylococcus aureus
– Streptococci (Cocci in chains)
Ex.- Streptococcus pyogenes
– Tetrad (Cocci in group of four)
Ex.- Micrococcus spp.
– Sarcina(Cocci in group of eight) 3
1. TRUE BACTERIA
5
•
6
Bacilli – These are rod-shaped bacteria. On the basis of
arrangement of organisms, they can be described as
– Monobacilli
– Diplobacilli
– Streptobacilli
– Palisades
– Coccobacilli
2. Spirillum
These are small, regularly coiled, rigid organisms
measuring about 3–4 um in length.
Each coil measures about 1 um.
Spirilla are motile with groups of flagella at both ends.
example - Spirillum minus.
8
3. Spirochaetes
These are relatively longer, slender, non-branched
microorganisms of spiral shape having several coils.
9
4. vibrios
These are small slightly curved rods measuring 3–4
um in length by 0.5 um in width.
Most vibrios are motile with a single flagellum at
one end.
e.g. Vibrio cholerae.
5. Mycoplasmas
• These bacteria lack in rigid cell wall (cell
wall lacking) and are highly pleomorphic
and of indefinite shape.
• They occur in round or oval bodies and in
interlacing filaments.
10
11
6. ACTINOMYCETES (actin- ray, mykes-fungus)
These are rigid organisms like true bacteria but they resemble
fungi in that they exhibit branching and tend to form filaments.
They are termed such because of their resemblance to sun
rays when seen in tissue sections.
Based on Anatomical features
•
•
•
Capsule
– Capsulate– Streptococcus pneumoniae
– Non-capsulate – Viridans streptococci
Flagella
– Flagellate –
• Monotrichous
• Lophotrichous
• Amphitrichous
• Peritrichous
– Aflagellate – Shigella spp.
Spore
– Spore-forming – Bacillus spp.
– Non-sporing – Escherichia coli
10
Based on Staining reaction
13
•
GRAM’S STAIN
Gram-positive –
– Staphylococcus aureus
– Clostridium spp.
Gram-negative –
– Neisseria gonorrhoeae
– E. coli
•
ACID FAST STAIN
Acid-fast bacteria
– Mycobacterium tuberculosis
Non-acid-fast bacteria
– Staphylococcus aureus
Based on Cultural characteristics
14
• Extra growth factorsrequirements
– Fastidious–Hemophilus influenzae
– Non-fastidious– Escherichia coli
• Hemolysison SheepBloodAgar
– Alpha-hemolysis–Streptococcus pneumoniae
– Beta-hemolysis– Streptococcus pyogenes
• Utilization of carbohydrates
– Oxidative - Micrococcus
– Fermentative– Escherichia coli
Based on Nutrition
15
• Autotrophs
• Heterotrophs
Based on environmental factors
16
• Temperature
• Oxygendependence
• pH
• Saltconcentration
• Atmospheric pressure
Temperature
17
• Psychrophiles(15-200C)– Pseudomonas
fluorescens
• Mesophiles(20-400C)– Escherichia coli,
Salmonella enterica,Staphylococcusaureus
• Thermophiles(50-600C)- Bacillus
stearothermophilus
• Extremelythermophiles(ashigh as 2500C)
Oxygendependence
18
• Aerobe (grow in ambient temperature,which
contains 21%O2and asmall amount of CO2,
0.03%)
• Obligateaerobes– Strictly require O2for their
growth (Pseudomonasaeruginosa)
• Microaerophilic (grow under reduced O2,5-
10%and increased CO2,8-10%)-Campylobacter
jejuni,Helicobacter pylori
Oxygendependence
19
• Facultativeanaerobe(capable of growing
either in presence or absenceof O2)-E.coli
• Obligateanaerobe– Clostridium spp.
• Capnophilic(require increased concentration
of CO2,i.e., 5-10%) –
H. influenzae,
N. gonorrhoeae
• Aerotolerant 19
•
•
•
•
pH
20
• Acidophiles(Lactobacillus acidophilus)
• Alkaliphiles (Vibrio)
• Neutralophiles(pH 6-8)
Majority of the medically important bacteria grow
best at neutral or slightly alkaline reaction (pH
7.2-7.6)
Saltconcentration
21
• Halophiles
• Non-halophiles
22

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Classification of bacteria

  • 1. Mr. Hemant Singh Kurmi Assitant Professor hemantkurmi05@gmail.com 7697614376 Institute of Pharmaceutical Sciences
  • 2. Basis of classification • Phenotypic classification Morphological Anatomical Staining Cultural characteristics Nutrition Environmental factors Biochemical reactions Antigenic structure • Genotypic classification DNA-DNA hybridization G+C content 2
  • 3. Morphological classification S.N Type of Bacteria Shape 1 True Bacteria I) -Coccus Round II) -Bacillus Rod 2 Spirillum Small, spiral 3 Spirochete Longer, slender-spiral 4 Vibro Coma (,) 5 Mycoplasm Irregular 6 Actinomycine Sun Light ray Bacteria canbe classified into sixmajor groups on morphological basis.
  • 4. • Cocci – These are spherical or oval cells. On the basis of arrangement of individual organisms they can be described as – Monococci (Cocci in singles) Ex.- Monococcus spp. – Diplococci (Cocci in pairs) Ex.- Streptococcus pneumoniae – Staphylococci (Cocci in grape-like clusters) Ex.- Staphylococcus aureus – Streptococci (Cocci in chains) Ex.- Streptococcus pyogenes – Tetrad (Cocci in group of four) Ex.- Micrococcus spp. – Sarcina(Cocci in group of eight) 3 1. TRUE BACTERIA
  • 5. 5
  • 6. • 6 Bacilli – These are rod-shaped bacteria. On the basis of arrangement of organisms, they can be described as – Monobacilli – Diplobacilli – Streptobacilli – Palisades – Coccobacilli
  • 7. 2. Spirillum These are small, regularly coiled, rigid organisms measuring about 3–4 um in length. Each coil measures about 1 um. Spirilla are motile with groups of flagella at both ends. example - Spirillum minus.
  • 8. 8 3. Spirochaetes These are relatively longer, slender, non-branched microorganisms of spiral shape having several coils.
  • 9. 9 4. vibrios These are small slightly curved rods measuring 3–4 um in length by 0.5 um in width. Most vibrios are motile with a single flagellum at one end. e.g. Vibrio cholerae.
  • 10. 5. Mycoplasmas • These bacteria lack in rigid cell wall (cell wall lacking) and are highly pleomorphic and of indefinite shape. • They occur in round or oval bodies and in interlacing filaments. 10
  • 11. 11 6. ACTINOMYCETES (actin- ray, mykes-fungus) These are rigid organisms like true bacteria but they resemble fungi in that they exhibit branching and tend to form filaments. They are termed such because of their resemblance to sun rays when seen in tissue sections.
  • 12. Based on Anatomical features • • • Capsule – Capsulate– Streptococcus pneumoniae – Non-capsulate – Viridans streptococci Flagella – Flagellate – • Monotrichous • Lophotrichous • Amphitrichous • Peritrichous – Aflagellate – Shigella spp. Spore – Spore-forming – Bacillus spp. – Non-sporing – Escherichia coli 10
  • 13. Based on Staining reaction 13 • GRAM’S STAIN Gram-positive – – Staphylococcus aureus – Clostridium spp. Gram-negative – – Neisseria gonorrhoeae – E. coli • ACID FAST STAIN Acid-fast bacteria – Mycobacterium tuberculosis Non-acid-fast bacteria – Staphylococcus aureus
  • 14. Based on Cultural characteristics 14 • Extra growth factorsrequirements – Fastidious–Hemophilus influenzae – Non-fastidious– Escherichia coli • Hemolysison SheepBloodAgar – Alpha-hemolysis–Streptococcus pneumoniae – Beta-hemolysis– Streptococcus pyogenes • Utilization of carbohydrates – Oxidative - Micrococcus – Fermentative– Escherichia coli
  • 15. Based on Nutrition 15 • Autotrophs • Heterotrophs
  • 16. Based on environmental factors 16 • Temperature • Oxygendependence • pH • Saltconcentration • Atmospheric pressure
  • 17. Temperature 17 • Psychrophiles(15-200C)– Pseudomonas fluorescens • Mesophiles(20-400C)– Escherichia coli, Salmonella enterica,Staphylococcusaureus • Thermophiles(50-600C)- Bacillus stearothermophilus • Extremelythermophiles(ashigh as 2500C)
  • 18. Oxygendependence 18 • Aerobe (grow in ambient temperature,which contains 21%O2and asmall amount of CO2, 0.03%) • Obligateaerobes– Strictly require O2for their growth (Pseudomonasaeruginosa) • Microaerophilic (grow under reduced O2,5- 10%and increased CO2,8-10%)-Campylobacter jejuni,Helicobacter pylori
  • 19. Oxygendependence 19 • Facultativeanaerobe(capable of growing either in presence or absenceof O2)-E.coli • Obligateanaerobe– Clostridium spp. • Capnophilic(require increased concentration of CO2,i.e., 5-10%) – H. influenzae, N. gonorrhoeae • Aerotolerant 19 • • • •
  • 20. pH 20 • Acidophiles(Lactobacillus acidophilus) • Alkaliphiles (Vibrio) • Neutralophiles(pH 6-8) Majority of the medically important bacteria grow best at neutral or slightly alkaline reaction (pH 7.2-7.6)
  • 22. 22