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GRM 2013: Validation of ZmMATEs as Genes Underlying Major Al Tolerance QTLs in Maize -- C Guimarães (redacted)


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These slides are a redacted (by the presenter) version of what was presented at the meeting

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GRM 2013: Validation of ZmMATEs as Genes Underlying Major Al Tolerance QTLs in Maize -- C Guimarães (redacted)

  1. 1. Embrapa Maize & Sorghum Jurandir Vieira Magalhães Lauro Guimarães Maria Marta Pastina Sidney Netto Parentoni Ubiraci Gomes P. Lana Vera Maria Carvalho Alves Christiano Simões Renato Vasconcellos Carlos Fasane Tinoco USDA-ARS/Cornell University - USA Leon Kochian Lyza Maron Miguel Piñeros Ed Buckler Moi University Kenya Samuel Gudu Beatrice Were Evans Ouma Thomas Matonyei KARI, Kitale Kenya Dickson Ligeyo Validation of ZmMATEs as Genes Underlying Major Al Tolerance QTLs in Maize G7010.03.02 Claudia Guimarães
  2. 2. Tropical Acid Soils High Al toxicity; low P availability Low sustainability for crop production in 48% of world arable land Represent: 205 millions ha, mostly arable lands in Brazil 7.5 millions ha in Kenya, coincident with maize growing areas Acid soilsMaize growing areas
  3. 3. Objectives Objective 1: Validation of functional ZmMATE or Al tolerance QTLs in Brazilian maize crosses Objective 2: Validation of ZmMATE genes or Al tolerance QTLs in a panel of Kenyan and Brazilian maize lines Objective 3: Development of molecular tools for markers assisted introgression of ZmMATE genes or Al tolerance QTLs
  4. 4. ZmMATE1: Candidate Gene for Al Tolerance in Maize Maron et al. 2010 Plant Journal 61: 728–740 • Homologue to SbMATE • Co-localizes to the qALT6 • High expression in root tips of Al-tolerant line under Al stress • Mediates citrate exudation • Improve Al tolerance and induces citrate exudation intransgenic Arabidopsis
  5. 5. High ZmMATE1 expression is associated with three copies of the target gene Maron et al. 2013. PNAS 110 (13): 5241-46 • qALT6 co-localizes with eQTL for ZmMATE1 expression • Three copy-number variation: improve ZmMATE1 expression and Al tolerance in RILs and NILs • Rare CNV: two Cateto lines and IL677 – maize diversity panel Three copies of ZmMATE1 in tandem: Cateto Al237
  6. 6. Molecular Tools for Marker-Assisted Introgression of qALT6 – ZmMATE1 • Source of high ZmMATE1 expression in adapted lines from Brazil and Kenya: • Kenya: CatetoAl237xL3-5, SynAlxR12C10-8, SynAlxAO89xAO89-40 and ATPS4SynxR12C10-5 • Brazil: Cateto Al237, L228-3, 521236, 521237 • Flanking markers for qATL6: • umc1018 and ZmMATE1 have been used to introgress qATL6 in maize NILs and other maize elite lines from Brazil; • SNPs PZA00606_4 located at 2.4 Mbp and PHM15961_13 at 9.7 Mbp on maize chromosome 6: molecular data available at IBP • Markers along maize genome: 1085 polymorphic SNPs among 38 Kenyan and 48 Brazilian lines: molecular data available at IBP
  7. 7. Key Products 1. Genetic materials developed and characterized for Al tolerance, ZmMATE1 expression and CNV, grain yield: RILs, NILs and near- isogenic hybrids introgressed with qALT6, Kenyan and Brazilian maize lines 2. Flanking markers for ZmMATE1: two flanking markers of qATL6 in Brazilian germplasm and two SNP markers genotyped in Kenyan and Brazilian lines. 3. Polymorphic SNP markers randomly distributed in maize genome. They can be easily selected in the IBP and used to recovery the recurrent genetic background in MABC programs. 4. ZmMATE1 validated for Al tolerance in maize: ZmMATE1 as a target region to improve Al tolerance and yield stability in maize.
  8. 8. Additional Products • Capacity building: • One PhD student from Kenya trained at Embrapa • One PhD and one MS student from Brazil • Two undergraduate students • Publications: • Book chapters: three • Papers: two published; one almost submitted • Several internal technical documents: Embrapa • Abstracts. • Future perspective to continue the ZmMATE1 study in a broad range of tropical and subtropical CIMMYT lines: collaboration with Raman Babu, CIMMYT - Hyderabad.