We analyzed more than 40 different fossil wood taxa from the Ituzaingó Formation. Palaeoclimatic data was determined based on physiognomic anatomical features using multivariate anatomical analyses. These results were compared with other proxies, including Carlquist's index and Nearest Living Relatives (NLRs). The anatomical features of the fossil wood analyzed show a tendency to xerophytic and seasonal vegetation. The comparison with the living relative suggests that the Ituzaingó Formation flora has elements linked with Chaco, riparian, and components of Seasonally Dry Tropical Forest (SDTF)