6. The experiment
In this experince we will learn to recognize the
presence of sugar(simple and complex) and proteins
using some reagents that change their colour in
presence of a specific nutrient.
7. Materials Required
Watch glass
Test tubes
Support for tubes
Bechers
Droppers
Mortar with pestle
Hotplate
Reagents: Fehling A and B, Lugol, Biuret.
Two kinds of food
8. The reactives that we
will use are:
Fehling’s solution
to identify the presence of
simple sugars
Lugol to identify
the presence of
starch
Biuret to identify
the presence of
proteins
9. Fehling A stirred with Fehling B gives a blue reagent
solution .
In the presence of simple sugars , the blue solution changes
its colour into green, yellow and brick-red, depending on
the amount of sugar.
Warning
Fehling B solution is dangerous.
Care needs to be taken to avoid conctact with skin.
10. Lugol reagent is a yellow Iodine solution that
changes its colour into blue –black when it reacts
with starch .
11. Biuret reagent is a blue solution that changes its
colour into pink-purple when it reacts with proteins.
Warning
Biuret solution is dangerous.
Care needs to be taken to avoid conctact with skin.