2. Lepidoptera: Noctuidae
Striacosta albicosta (Smith), Arizona, 18871, 2.
Occurred in Colombia, Mexico, SW United States
Outlying records: southern Idaho and Iowa
Western bean cutworm
Photo by J. Obermeyer, Purdue
1 Smith 1887, 2LaFontaine and Gill 2004
3. WBC Range Expansion
Grey – Original Range
Yellow – Expansion since 2000
From: Ecology and Management of the
Western Bean Cutworm (Lepidoptera:
Noctuidae) in Corn and Dry Beans J Integr
Pest Manag. 2010;1(1):A1-A10.
doi:10.1603/IPM10003
4. Larval Behaviour in Corn vs Beans
• WBC behave differently in corn vs. beans
• In corn, you can see all stages of the insect during
the day
– Easily find eggs on leaves or larvae in ear later in the
season
• In beans, only young larvae active during the day.
Mid to late instars active at night, moving plant to
plant each night to feed. Hide in ground during day
– Very difficult to find any life stage on the plant prior to pod
feeding/holes appearing
6. Pupation and Moth Emergence
• Larvae drop to the ground in fall to make pre-pupal chamber
• Stay all winter in pre-pupal stage
• Pupate in soil in May and June
• Moths begin to emerge from pupae in Ontario soils in early
July (UGRC, 2011-2013)
• Emergence occurs earlier upwind
from Ontario since traps begin to
catch moths in early June
• Emergence is staggered, given that
trap catches continue for over three
months
8. Migrant and Resident Populations
• Carried in via weather fronts from neighbouring
states, prior to our resident population emerging
• Resident moths also fly and get carried across
Ontario, into Quebec, NY and Atlantic provinces
(PEI and Nova Scotia confirmed in 2017)
9. Adult Moths
• Moths present in Ontario from June until early
September, with avg. peak flight in late July
• Females emerge first, reach sexual maturity in 4-6
days and mate approx. 3 times
• Lay 3- 400 eggs in lifespan
• Average lifespan of males and females is 7 and 9
days, respectively
10. Moths fly at night but
you might find them
resting in the leaf
axils during the day
MSU research found
that the bulk of
moths were flying
around between
1 am – 3 am
T. Baute, OMAFRA
11. J. Smith, UGRC
WBC Moth Identification
• Approximately 2 cm in length
• White band along wing margins
• Full moon and boomerang markings on each wing
• Wing folds over the other, almost lining up with each other
at the tip
14. Corn Growth Stage is Key
• If there is no tassel inside the whorl, the newly
hatched larvae die within a day or two
• Eggs laid on young corn plants will die
• Focus on fields that at least have a tassel
developing inside the whorl
• Cues for adults from tassel and silk tissue?
• Variable growth stages in fields complicate
scouting
15. WBC Eggs
• Egg masses have an average of 50-84 eggs
• Longitudinal striations like cantaloupe
• Pearly-white when fresh
• Hatch in 5 to 7 days
(temp. dependant)
• Peak egg laying shortly
after peak flight
16. Assessing age of egg mass
Day 1 2 3 4 5 6 7
Pearl
white
Light
tan
PurpleDark
tan
Hatch
C. DiFonzo, MSU
17. Larvae
• Goes through 6 instars in approx. 30 days
• ~2 mm (1st) to ~34 mm (6th) in length
• Initially feed on their eggshell, then can only
survive on tassel, pollen (flower), silks or kernels
(pod and seeds) – little to no leaf feeding
• Not cannibalistic – can have multiple per ear
• Actively move from plant to plant
18. Last Instars
5th and 6th instars
T. Baute, OMAFRA
Two distinct bands
behind head
19. 10 feet across rows
Larval dispersal
Infestations can
be spotty!
21. Economic injury by WBC
Yield loss of 3.6-15 bu/ac with
one larva per plant
Grain quality loss
Secondary pests
Mycotoxins
C. DiFonzo, MSU
C. DiFonzo, MSU
22. The pest complex
Western Bean
Cutworm
Fusarium Ear Rot/Gibberella Ear Rot
& Mycotoxins
Injury
Silk
23. What are Mycotoxins?
Mycotoxins = toxic metabolites of fungi
Mycotoxin Commodity Fungal source(s) Effects of ingestion
Deoxynivalenol (DON)
(Vomitoxin)
Wheat, corn, barley
Fusarium graminearum Human toxicoses India,
China, Japan, and Korea.
Toxic to animals,
especially pigs.
Fusarium crookwellense
Fusarium culmorum
Zearalenone (ZEN) Corn, wheat F. graminearum Possible human
carcinogen (IARC). Affects
reproductive system in
female pigs.
F. culmorum
F. crookwellense
Ochratoxin A (OTA) Barley, wheat, and many
other commodities
Aspergillus ochraceus Suspected by IARC as
human carcinogen.
Carcinogenic in laboratory
animals and pigs.
Penicillium verrucosum
Fumonisin B1 Corn Fusarium moniliforme plus
several less common
species
Suspected by IARC as
human carcinogen. Toxic
to pigs and poultry. Cause
of ELEM, a fatal disease of
horses.
Aflatoxin B1, B2
Corn, peanuts, and many
other commodities
Aspergillus flavus Potent human carcinogens
by IARC. Adverse effects in
various animals, especially
chickens.
Aflatoxin B1, B2, G1, G2 Corn, peanuts Aspergillus parasiticus
http://www.fao.org/wairdocs/x5008e/x5008e01.htm
24. Risks associated with mycotoxins in grain
Feed quality
Detrimental effects on livestock
Ethanol production
Initial mycotoxin concentration is concentrated by 3X in DDGS
Trade and marketing implications
Rejection
Downgraded grain
Financial penalties
Mycotoxin Commodity Canada Commodity USA
Deoxynivalenol
(mg/kg=ppm)
Uncleaned soft
wheat for human
consumption
2 ppm Finished wheat products 1 ppm
Deoxynivalenol Diets for cattle &
poultry
5 ppm Grains and grain by-products
destined for ruminating beef and
feedlot cattle older than 4 months
and chickens (not exceeding
50% of the cattle or chicken total
diet)
10 ppm
Deoxynivalenol Diets for swine,
young calves, &
lactating dairy
animals
1 ppm Grains and grain by-products (not
exceeding 40% of the diet)
5 ppm
www.inspection.gc.ca/animals/feeds/regulatory-guidance/rg-8/eng/1347383943203/1347384015909?chap=1#s1c1
25. Deoxynivalenol (DON) in Ontario corn
Frequent issue in Ontario grain corn
DON 2011 2012 2013 2014 2015 2016 2017
<0.5 ppm
75%
85% 84% 66% 75% 48% 69%
0.5-<2.0 ppm 11% 14% 25% 20% 26% 17%
≥2.0 ppm 24% 4% 2% 9% 5% 26% 14%
OMAFRA Survey, A. Tenuta and B. Rosser 2017
45% samples > 3ppm
28. Insects and mycotoxins
Fall armyworm,
corn earworm
& fumonisins
Sap beetles
European corn borer
J. Obermeyer, Purdue
J. Obermeyer, Purdue P. Porter, TAMU
29. ECB and DON
Bt corn reduced DON by ~60% when infested with high
intensity (>4 cm stalk tunneling) of ECB
DON (ppm)
1996 1997 1998 1999 Avg
Non-Bt isoline 1.25 0.51 1.15 1.19 0.96
Bt 0.45 0.36 0.69 1.06 0.57
P-value <0.0001 0.02 0.0019 0.51 <0.0001
Schaafsma et al. 2002 Plant Disease
34. Insecticide Efficacy
2011: 2 sites with 77 and 85% plants with WBC egg masses
Field-scale plots (3.6 × 18 m), 4 reps, RCBD, Non-Bt or Cry1Ab corn
Applied at early VT (~90% tassel emergence) with high-clearance
sprayer
WBC Injury
No significant differences
in DON or yield
Incidence 72-88%
Severity 75-95%
40. Treatment effect, no Bt effect
Observed greater reduction in DON with insecticide than fungicide
Low DON levels, fungicide-only treatments may have been applied too
late (late R1-R2), silk infection occurred and insect injury not controlled.
No difference in DON due to application timing of insecticide + fungicide
Effect on total DON
(p<0.05, ANOVA, Tukey-Kramer)
44. Trapping for WBC
• Indicate moth presence in a region
• Indicate when peak flight and peak egg laying
likely
• Trap counts do not determine if threshold has
been reached (only catching males)
• Very important for areas outside the original hot
spots to trap
• >2500 trap locations over 10 years, captured
over 858,000 WBC moths in Ontario!
51. Scouting in Corn for WBC
• If traps are catching moths, focus on fields in the
pre-tassel to full tassel stages
• Scout 10 plants in ideal growth stage, in 10 areas of
the field (100 plants each time)
• Scout every 5 to 6 days, for at least 3 weeks
• Count the # of plants with egg masses
• Accumulate # of plants over the 3 week period
• Be area of variable growth stages in each field.
Focus scouting in the areas of the field in the ideal
stages
52. Walk corn rows with plants between you and the sun. Eggs leave shadows!
54. Adjusted WBC Corn Threshold
• Previous threshold and timing were:
– 5% of plants scouted with egg masses and/or
small larvae (decreased from 8% in Nebraska)
– spray at full tassel when majority of egg masses
have hatched
55. Adjusted WBC Corn Threshold
• Previous threshold and timing were:
– 5% of plants scouted with egg masses and/or small
larvae
– spray at full tassel when majority of egg masses have
hatched
• Modified threshold for Great Lakes
– cumulative 5% threshold – when 5% of the plants over
a two or three week period have had egg masses
and/or small larvae
– even lower threshold likely needed, if growing a
Fusarium susceptible hybrid
56. Adjusted WBC Spray Timing for Corn
• Pre-tassel to full tassel stage has been too early
– Prolonged moth flight and egg laying
• WBC only feed on tassel, silks and kernels – can’t
live on tassel tissue for long
• All WBC larvae will head to the ear when present
• Instead, spray when fresh silks are present,
targeting the ear zone (ground application only?)
• Can tankmix with fungicide for ear mould
protection at the same time
57. Products Registered on Corn in Canada
• Matador or Silencer (pyrethroid)
• 83 – 187 mL/ha
• 14 days pre-harvest interval silage, 21 days for field corn
• Coragen (diamide)
• 250 – 375 mL/ha
• 14 day pre-harvest interval
• Delegate (spinosyns)
• 120 – 210 grams/ha
• 28 days pre-harvest for stover, 14 days for forage
• Voliam Xpress (pyrethroid + diamide)
• 500 mL/ha
• 7 days pre-harvest interval
• ROTATE chemistries – high risk of resistance with WBC
58. Transgenic Corn Options
• Cry 1F (Herculex or SmartStax) no longer
provides protection against WBC
• Only hybrids with Vip3A (Viptera) provides
control
59. Evidence for resistance of Western bean
cutworm to Cry1F Bacillus thuringiensis
protein and transgenic corn hybrids in
Ontario, Canada
J. L. Smith1, M. D. Lepping2, D. M. Rule2, Y. Farhan1, and A. W. Schaafsma1
1 Department of Plant Agriculture, Ridgetown Campus, University of Guelph, 120 Main
St. E., Ridgetown ON, Canada N0P 2C0.
2 Dow AgroSciences LLC, 9330 Zionsville Rd. Indianapolis, IN 46268
62. Cry1F susceptibility bioassays
Adults collected at black light traps or egg masses from the field
in late July 2015
5 locations in SW ON
Dose-response diet-overlay bioassay
(Marcon et al. 1999, Dyer et al. 2013)
Truncated Cry1F (53% a.i.) from Dow AgroSciences
Infested with neonates, 24 larvae/conc/rep x 3 reps
F0: 0 to 30,000 ng ai cm-2
Mortality at 7 and 14 d
Weight of surviving larvae at 14 d
F1: 0 to 75,000 ng ai cm-2
Mortality and weight at 7 d
70. Resistance management for WBC
Challenges:
• Reliance on 1-2 insecticides
• Slow integration of Vip3A into widely used hybrids
• Vip3A deployed with a 5% integrated refuge
• Almost no effective refuge due to cross-pollination
• Extensive inter- and intra-plant larval movement
• Exposure of later instars to sublethal doses
Solutions(?)
• More insecticide options needed
• Tank-mixes, rotation program
• Stewardship of Vip3A
• Abandon integrated refuge
• Require insecticide treatment
• More high-dose transgenic solutions