Comparative evaluation of Physical and Chemical mutagens

1. Comparative Evaluation Of Physical And Chemical Mutagens For Creation Of
Variability In The Same Species – Case Studies
PRESENTED BY
S.DIVYA
TAMIL NADU AGRICULTURAL UNIVERSITY,
COIMBATORE- 641003

2. Casestudy1

3. Gladiolus
 Gladiolus is known worldwide for its attractive spikes and has gained much importance as
‘Queen of bulbous flowers’
 The demand of gladiolus is increasing therefore, it needs attention towards genetic
improvement. Improvement in any crop depends on the variability available in it
 Variability can be created through mutation breeding

4. Materialsandmethods
 Corms of gladiolus variety ‘Psittacinus Hybrid’ having diameter of 5-10 cm were treated with
different doses of gamma rays (5.5, 6.0, 6.5, 7.0, 7.5, and 8.0 kR) and different concentrations of
0.1, 0.2, 0.3, 0.4 and 0.5% Diethyl Sulphonate (DES) for 3 hours.
 After the treatment, these corms were immersed in STS solution for 15 minutes
 Then washed in running tap water for 20 minutes. Then the corms were planted on the raised
beds
 The data were recorded on growth and flowering parameters and statistically analyzed

5. Observationsrecorded
Vegetative parameters
 Sprouting percent,
 Survival percent,
 Number of tillers,
 Plant height and
 Leaf area
Flowering parameters
 Days to flowering,
 Spike duration,
 Number of spikes/plant,
 Spike length,
 Rachis length,
 Number of florets/spike and
 Vase life

6. ComparativeEffectOnVegetativeparameters

7. ComparativeEffectOnFloralparameters

11. Results
 Different mutagens like gamma rays and DES, could significantly influence the various vegetative,
flowering and yield parameters and also induced some ornamental variants in gladiolus variety
‘Psittacinus hybrid’.
 Corms treated with DES upto 0.3% produced different favourable variations and abnormalities.
 Floral abnormalities were also found when irradiated with gamma rays but not any desirable
variation was recorded.
 Colour novelties were produced with different concentrations of DES. Even the quality of spikes
improved due to increased spike length and girth of spike at 0.2% and 0.3% DES. Overall, it can be
said that DES was more effective as compared to gamma rays for induction of variability.

12. Casestudy2
 Bhajantri Archana, (2013) Genetic Variability And Improvement Studies In Gladiolus
(Gladiolus Hybrids Hort.)

13. Materialsandmethods
 Two gladiolus genotypes (Gladiolus hybrids Hort.) viz., Ethyl Cav Cole and White Prosperity
were selected for mutagenic treatments
 Corms of gladiolus genotypes were treated with chemical mutagen, ethyl methane
sulphonate (EMS-CH3CO2OC2H5)- (EMS 0.25%, 0.50% and 0.75%)) and physical mutagen,
gamma rays (60Co) -(10 Gy, 20 Gy and 30 Gy).

15. Observationsrecorded
M1VI generation
 Sprouting percent,
 Survival percent,
 Number of tillers,
 Plant height and
 Leaf length
 Leaf width
M1V2 generation
 Weight of the corm,
 Diameter of the corm,
 Days to flowering,
 Spike duration,
 Number of spikes/plant,
 Spike length,
 Rachis length,
 Number of florets/spike and
 Spike girth

24. Results
 Use of lower doses of chemical mutagens (EMS 0.25% ) that do not cause drastic chromosomal
damage may be more effective in increasing the amount of variability.

25. References
 Dhawani Patel1, (2018) Sudha Patil, Sanket J. More and Trupti P. Dohiya, Comparative Effect of Physical
and Chemical Mutagens in Inducing Variability in Gladiolus Variety ‘Psittacinus Hybrid’ International
Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01
 Bhajantri Archana, (2013) Genetic Variability And Improvement Studies In Gladiolus (Gladiolus Hybrids
Hort.)

26. THANK YOU