Malinda Madusanka presented on bioengineered microbes at the University of Sri Jayewardanepura. Bioengineering involves applying engineering principles to modify organisms, such as genetically engineering microbes. Madusanka discussed how microbes can be engineered for applications like bioremediation, fuel production, and manufacturing chemicals. Some advantages are using microbes to degrade waste, convert CO2 to fuel, and produce vaccines. Challenges include high costs, lack of expertise, and potential environmental impacts. Madusanka proposed ways bioengineered microbes could be applied in Sri Lanka, such as wastewater treatment, fish fermentation, and renewable energy production.

1. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Bioengineered Microbes
Malinda Madusanka
3rd Year – 1st Semester
Food Science & Technology Department

2. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
I am Malinda Prabhath
Madhusankha from
Maliyadeva college.
I always believe that if we start
anything with pure heart it will
become successful.
I like social service and appreciate team work a lot.
I was a member of our school cricket team.

3. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Overview (Expected Areas to Cover)
Introduction
• Background
• Definition
 Applications
• Process
 Advantages and Disadvantages
 How to apply in Sri Lanka
 References

4. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Introduction
History
• The word” bioengineering” was introduced by
British scientist and broadcaster Heinz Wolff in
1954.
• The first biological engineering program was
launched at Missisipi State university in 1967 and
it paved the way for initiating bioengineering
programmes throughout the world.

5. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
What are bioengineered Microbes?
• The process of applying biological techniques
(as genetic recombination) to create modified
versions of organisms is called bioengineering.
• Here the microbes are subjected to engineering
applications along with biological concepts and
the ultimately created organism is called a
”bioengineered microbe”.

6. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Process
• Identification of the relevant gene.
• Isolation of that particular gene.
• Amplifying the gene to produce many
copies.
• Insertion into plasmids.
• Multiplying the plasmids in bacteria .
• Recovering the cloned construct for
injection.
• Transferring the construct into the recipient
tissue.

7. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
• Usually fertilized eggs are used as recipient
tissues.
• Integration of gene into the recipient
genome.
• Expression of the gene in recipient genome.
• Finally inheritance of the gene through
future generations.

8. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Advantages
• Bioremediation
Using engineered microbes and their enzymes to
promote degradation.
• Fuel production
Bioengineered microbes can be used to convert
CO2 and waste into gasoline compatible
transportation fuel

9. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
• Production of chemicals
Producing chemicals of commercial interest or
biomedical importance such as insulin.
• Use in medicinal purposes
Lactobacillus Species present in gastrointestinal
tracts are used to prevent diarrhoea and urinary
infections.
• Producing vaccines to treat tumours
Genetically modified yeast species are used to
stimulate the immune system.

10. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
• Microbial fuel cell.
It is a bio electrochemical system where
current is driven using bacteria.
• Fermentation
Genetically modified saccharomyces species
are used in wine production to get a high
yield.

11. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Disadvantages
• This process is very expensive and so many
researches should be done prior to the usage.
• Lack of scientists with adequate knowledge
and training.
• The risk of occurring mutations .
• It will affect the balance of the nature in an
adverse manner.
• Sometimes this technique may be used for
manufacturing bioweapons.

12. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
How to apply in Sri Lanka
• Can be used in waste water treatment in
urban areas.
• For the fermentation of fish
Sri Lanka is a country where a high yield of fish is
gained. So bioengineered microbes can be used
to ferment these fish and produce fish sauce.
• For the production of renewable energy
Genetically modified anaerobic bacteria is used to
produce ethanol from syngas.

13. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
• Scientists have invented a group of microbes to
glow in the presence of landmines.

14. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
Possible Applications in the Food Industry
• In bakery industry and brewery industry
bioengineered saccharomyces cerevicea
can be used.
• Fungi species can be bioengineered to
gain a high yield in cheese production.
• In yoghurt industry Lactobacillus lacti
and Lactobacillus bulgaricus can be
used after genetical modifications.

15. Emerging Food Technologies Presentation Series - 2016
University Of Sri Jayewardanepura
References
www.scirp.org
www.sciencedirect.com
www.livescience.com
www.nature.com
www.geek.com