Dna Metabarcodding

1. METABARCODDING

2. METABARCODDING
• Metabarcoding is the barcoding of DNA/RNA (or eDNA/eRNA) in a
manner that allows for the simultaneous identification of many taxa
within the same sample

3. METABARCODDING
• The metabarcoding procedure, like general barcoding, proceeds in
order through stages of DNA extraction, PCR amplification,
sequencing and data analysis.

4. ENVIRONMENTAL DNA
• Environmental DNA or eDNA describes the genetic material present in
environmental samples such as sediment, water, and air, including
whole cells, extracellular DNA and potentially whole organisms.
• eDNA may come from skin, mucous, saliva, sperm, secretions, eggs,
feces, urine, blood, roots, leaves, fruit, pollen, and rotting bodies of
larger organisms, while microorganisms may be obtained in their
entirety

5. Global ecosystem and biodiversity
moitoring with eDNA

6. Application of eDNA
metabarcodding

7. Metabarcodding stage

8. the appropriate techniques should be
chosen for DNA sequencing.

9. Metaborcodding
workflow

10. Metabarcodding
workflow

11. Method and visualisation
• Alpha diversity displayed as taxonomy bar charts, showing relative
abundance of taxa across samples using the Phinch data visualization
framework (Bik & Pitch Interactive 2014).
• Beta diversity patterns illustrated via Principal Coordinate Analyses
carried out in QIIME,[47] where each dot represents a sample and
colors distinguish different classes of sample. The closer two sample
points in 3D space, the more similar their community assemblages
• GraPhalAn phylogenetic visualization of environmental data, with
circular heatmaps and abundance bars used to convey quantitative
taxon traits

12. Method and visualisation

13. Application
Pollinator network

14. Disentangling
food webs

15. Marine biosecurity

16. Detection of non-indigenous species (NIS)