PROKARYOTIC DNA REPLICATION

1. PROKARYOTS DNA
REPLICATION
MOLECULAR BIOLOGY

2. • WHAT IS REPLICATION ? :
DNA replication is a process by
which a double- stranded dna
molecule is copied to produce
two identical molecules
INTRODUCTION
2
• PROKARYOTIC REPLICATION:
WHEN REPLICATION PROCESS
HAPPENS WITH THE CIRCULAR
DNA OF
PROKAROTIC(BACTERIAL CELL),
THEN IT IS CALLED
PROKARYOTIC REPLICATION

3. STEPS OF PROKARYOTIC DNAREPLICATION
* According to a Survey
3
STARTING OF
REPLICATION
ELONGATION
FORMATION OF
REPLICATION
FORK
TERMINATION
SEPARATION OF
PARENTAL AND
DAUGHTER DNA
PROKARYOTIC REPLICATION
INITIATION

4. ENZYMES
4
ENZYMES NEEDED
FOR PROKARYITIC
REPLICATION
DNA A
DNA B
DNA C
SSB
DNA G (PRINASE)
DNA polymerase III
RNAace H
Ligase
TUS protein

5. INITIATION
5

6. 6
1 2
2 3

7. ELONGATION
REPLICATION FORK
7

8. 8

9. TERMINATION
9

10. 10
SIGNIFICANCE OF PROKARYOTIC DNA REPLICATION
Prokaryotic Replication
This process occurs in prokaryotic cells.
It is a continuous process.
Circular, double-stranded DNA
The DNA replicates in the cytoplasm
Single origin of replication
Small amount of DNA
DNA polymerase I and III are involved
Large okazaki fragments
The process is rapid, 2000 base pairs per second
Two circular chromosomes are obtained
DNA gyrase is required

11. THANK
YOU