Here is the process of formation of progeny DNA from Parental DNa. The process is described here briefly. DNA, abbreviation of deoxyribonucleic
acid, organic chemical of
complex molecular structure that is found
in all prokaryotic and eukaryotic cells and
in many viruses. DNA codes genetic
information for the transmission of
inherited traits.
2. What is DNA?
DNA, abbreviation of deoxyribonucleic
acid, organic chemical of
complex molecular structure that is found
in all prokaryotic and eukaryotic cells and
in many viruses. DNA codes genetic
information for the transmission of
inherited traits.
3. DNA REPLICATION
In the process where using the two strand of Parental DNA as a template
and two progeny DNA are made, that is called DNA REPLICATION
-The process happens in the ‘S’ (Synthesis Stage) of Interphase
Place-
Nucleus , in case of Eucaryotic cell
Cytoplasm , in case of Procaryotic cell
4. Necessity of
1. Parental DNA
2. Tropoisomerase
3. Gyrase
4. Helicase
5. SSB protein
6. RNA Primer
7. DNA Polymerase
8. dNTP
9. Ligase
5. Process
The whole process happens through three stages. That are-
1. Initiation Stage
2. Elongation stage
3. Termination stage
1. Initiation Stage
• By the help of helicase 2 strands of parental DNA are separated by
hydrolyzing the Hydrogen bond
• SSB is attached with both Strand
• 10-12 Base pair RNA Primer is made by RNA Primase Enzyme
6. 2. Elongation Stage
• Starting place is known as ‘Replication of Origin’
• At 3’ End of RNA Primar, The complementary deoxy
ribonucleotides are joined by making Phospho-di-ester
bond by help of DNA Pol-III
• The strand, where DNA synthesis happens continuously,
known as leading strand. The strand, where DNA synthesis
does not happen continuously, known as lagging strand.
7. 3. Termination Stage
• Termination happens when the extended forks are made
• RNA Primars are hydrolyzed by DNA Pol-I, and deoxy
ribonucleotide is synthesized by help of DNA Pol-II
• 2 Oazaki Fragments are added by help of ligase.
