1. Contact No: 9405367843 / 9028729272
Email: kool.amol@gmail.com
Amol Martandrao Kulkarni
C/o P. Y. Kulkarni, Flat no 1, Anuyog Apartment, Nandigram Colony, Garkheda,
Aurangabad-431005, Maharashtra, India
As an experienced researcher so far; I would like to be a reason for the development and
advancement of the esteemed organization in which I would be working, using my skills,
expertise and learning to the best of my capability to raise the firm as well as myself to a
new level which would also satisfy my soul to be in research.
In vitro anti-infective evaluation:
Extensive hands on experience in microbiological evaluation of various NCEs from
variety of classes of antibacterial such as fluoroquinolones, oxazolidinones,
macrolides and ketolides.
Drug diffusion assays in agar media.
Spectrum determination by Minimum Inhibitory Concentration (MIC)
determination by agar and broth dilution method.
Modified agar dilution method for MIC determination in 96-culture format using
lids of 96 well microtitre plates to generate extensive data.
MIC determination for anaerobe cultures by using anaerobic chamber
Performing biofilm assays for Pseudomonas and Staphylococcus genera
Time kill studies for diverse pathogens
Determination of resistance selection window
Career Objective
ive:
Area of Expertise
ive:

2. Contact No: 9405367843 / 9028729272
Email: kool.amol@gmail.com
Enzyme inhibition assays (Determination of IC50)
In-vitro resistance development:
• To develop an In vitro resistance to differentiate the activities of NCEs and
antibacterial agents used in the clinics.
Molecular Biology Techniques:
• Genotyping by using PCR
• Isoelectric focusing (IEF) of ESBL
• PFGE
• SDS-PAGE
In vivo expertise
Extensive hands on experience in in vivo evaluation of various NCE s from variety of
classes of antibacterial such as fluoroquinolones, oxazolidinones, macrolides,
ketolides and beta lactams
� Animal handling and experimentation on mice, rat, rabbits and guinea pigs, etc.
� Viable counts of bacilli in organs of animals.
� In vivo efficacies of drugs against bacterial infections.
� Experience in development of animal models for basic screening of NCE�s
against variety of bacterial pathogens, such as:
1. Mouse peritonitis model
2. Staphylococcal septicemia
3. Pyelonephritis (UTI model)
4. Thigh infection
5. Skin abscesses
6. Bacterial endocarditis
7. Lung infection model
8. Tissue burden models
9. Bone and Joint Infection model

3. Contact No: 9405367843 / 9028729272
Email: kool.amol@gmail.com
Currently working as a Senior Research Associate (New Drug Discovery department) at
Wockhardt Research Center, Maharashtra, India since December 2008. The work in which I
am involved is all about developing a new broad spectrum antibacterial agents against
MDR bacterial infections.
Wockhardt Research Centre Dec 2008 – Till
Date
Aurangabad, Maharashtra
Current Designation – Senior Research Associate (New Drug Discovery department)
Key Roles & Responsibilities –
In-vitro studies:
-Primary screening of NCEs:
• To screen the New Chemical Entities (NCEs) at primary level by using Agar dilution MIC
method for their
Activity against clinical isolates harboring various resistance mechanisms.
• To compare the activity of NCEs with that of antibacterial agents used in clinics and which
are under
Development.
-Understanding the Resistance mechanism to antibiotics:
• To study and understand the different mechanisms of resistance in bacterial clinical
isolates.
-In-vitro resistance development:
• To develop an In vitro resistance to differentiate the activities of NCEs and antibacterial
agents used in the
Clinics.
Professional Experience
ive:

4. Contact No: 9405367843 / 9028729272
Email: kool.amol@gmail.com
In-vivo studies:
-Animal handling
-Inducing/developing Infections and dosing through various routes such as:
• Intravenous
• Intraperitoneal
• Intramuscular
• Intranasal
• Oral
• Subcutaneous
-In-vivo screening of NCEs
• Systemic infection studies
• Organ Eradication Studies (Lung, Kidney, Thigh)
-Development of New Murine Infection Models
 Philosophy of Doctorate (PhD)
Pursuing PhD in the subject of clinical Microbiology from Swami Ramanand Teerth
marathwada University, Nanded, Maharashtra. with the focus on “Studies on
development of resistance to Fluoroquinolones in Gram negative Bacterial
Pathogens”. Submitted the final synopsis and going to submit the thesis soon.
 Master of Science in Microbiology (M.Sc.) July2008
Swami Ramanand Teerth Marathwada University, Nanded,
Maharashtra, India.
Educational Qualification
ive:

5. Contact No: 9405367843 / 9028729272
Email: kool.amol@gmail.com
 Bachelor of Science in Microbiology, Chemistry and Botany (B.Sc. ) June2006
Swami Ramanand Teerth Marathwada University, Nanded,
Maharashtra, India.
 Senior secondary education May2002
Maharashtra Divisional Board of higher secondary
Education, Maharashtra.
Dr. Mahendra K. Ranjekar
Prof. in biology,
Dept. of Applied Sciences,
College of Engineering,
Pune #5, Maharashtra (India)
E-mail ID: mkr.appsci@coep.ac.in
Mob. : 09422015217
I hereby declare that the information given by me in this resume is true and up to date to
the best of my knowledge.
DATE:
PLACE: Aurangabad (Maharashtra)
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