A Series of Lectures By Mr. Allah Dad Khan Former DG Agriculture Extension KPK , Provincial Project Director CMP II MINFAl Islamabad and Visiting Professor the University of Agriculture Peshawar Pakistan

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Tissue Culture
A Series of Lectures
By
Mr. Allah Dad Khan
Former DG Agriculture Extension
KPK , Provincial Project Director
CMP II MINFAl Islamabad and
Visiting Professor the University of
Agriculture Peshawar Pakistan

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History
 1965
 French Botanist discovers Micropropagation
 Virus-free orchid plant
 Beginning of Tissue Culture

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What is Tissue Culture?
 Asexual Propagation
 aka Micropropagation
 Defined as:
 Production of new plantlets from tissue of an
actively growing plant

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Why use tissue culture?
 Species with any of the following:
 Regeneration problems
 Poor germination
 Desirable traits
 Disease-resistance
 High productivity
 Gender of commercial importance
 Male- Asparagus
 Female- Papaya

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Where is it used?
 Commercial Production
 Chrysanthemum
 Gladiolus
 Etc.
 Crop production
 Sugar cane
 Tapioca
 Potatoes

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What is required?
 Very sanitary conditions
 Usually a lab
 Sterile aseptic culture or MEDIA containing:
 Nutrients
 Sugars
 Vitamins
 Hormones
 Test tube
 Plant tissue:
 shoot tip, leaf section, meristem, node, etc.

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The Procedure
1. Prepare the media
2. Sterilize everything including:
Media
Tools
Supplies
Equipment
Work area
1. Place media in test tube
2. Remove tissue from plant

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Procedure continued
5. Sanitize tissue sample
6. Place tissue sample on media in test tube
7. Cap or seal test tube
8. Place in warm, well-lighted area
9. Plantlets should begin to form in 4-6
weeks

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Tissue Culture Process
Start
Tissue Removed
Media
CallusPlantlet

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Advantages Disadvantages
 Small part of plant is
required
 Produce many
plantlets in small
areas
 Disease-free plants
 Pest-resistant
 Maintaining a sterile
environment
 Expensive lab facilities
 Poor photosynthesis
 Water loss

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Guided Practice
 Group Activity:
 Put the steps for tissue culture in order
with your group.

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Independent Practice
1. What is another name for tissue culture?
2. Define tissue culture in your own words.
3. List one advantage and one disadvantage
of tissue culture.
4. What four materials are required for the
tissue culture process?

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