Setup of a tissue culture laboratory with esteemed budget.

1. Presented by
NILANJAN ADHIKARI
B.PHARM, 4th year 7th SEM
REG. NO.- 142420210054 OF 2014-15
ROLL NO- 24201914054
UNDER THE GUIDENCE
OF
MR. SUPRIYA DATTA (SR. ASST. PROF.)
OF
BENGAL COLLEGE OF PHARMACEUTICAL SCIENCES &
RESEARCH
AFFILIATED TO
MAULANA ABUL KALAM AZAD UNIVERSITY OF TECHNOLOGY (W.B)
2017

2.  DEFINATION OF PLANT TISSUE CULTURE
 LOCATION
 IDEAL UNIT FOR DECORATE PLANT TISSUE CULTURE LAB
 PURPOSES FOR THATS CHEMBER
 ESTIMATE DIMENTION
 DIAGRAMMATIC CONSTRUCTION
 GLASSWARE & OTHER EQUIPMENTS
 ESTIMATE BUDJET
 SHORT QUOTE VIDEO OF PROCESS OF “PLANT TISSUE
CULTURE”
 CONCLUSION
 REFERENCE

3.  Plant tissue culture is a collection of techniques used
to maintain or grow plant cells, tissues or organs under
sterile conditions on a nutrient culture medium of
known composition.[1]
 Plant tissue culture is widely used to produce clones
of a plant in a method known as Micro propagation.[3]
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4. The location shall confirm to the land use plan of
the area.
The building should be located away from
sources of contamination such as soil mixing area,
storage etc.
The site shall be accessible to service roads,
water supply and electric lines.
The site shall be well drained.
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5. A research facility should have at least four
chamber[1]:
Washing Area,
Media Preparation Room,
Sterile Area,
Growth/Culture Room,
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6. Washing area [6]
 for glassware washing
 storage and
 autoclaving.
Media preparation room
 for media preparation
Sterile area
 for aseptic manipulation
Growth & culture room
 to maintain cultures under suitable
environmental conditions
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7. [4],[6]
The glassware washing area shall be located near the
sterilization and media preparation areas.
Washing sink(45cm x 60cm) can be fitted in one side
of Room and should be acidic and alkali resistant.
Room also provided with Bucket, detergent , mug and
also Plastic tray to kept washed Media bottles and
sterilized media.
Room provided with water line and electricity line.
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8. The area shall be provided with working tables which should be up to
1.2 m wide and the height should be 850 mm – 900 mm. [5],[6]
 Table top shall be covered with materials which are easily cleaned and
which will stand disinfectant solution.
 Water source and glassware storage area shall be provided.
 Room contains double distilled water unit , pH meter, buffer solution,
electronic balance , cooling freeze, measuring cylinder, magnetic
stirrer, pipette, hot plate, media bottle.
 Room should contains the stock A,B,C,D, E and F solution with
required chemicals such as sucrose, adenine sulphate ,innositol , agar-
agar( solidifying agent) etc
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9. [3],[5]
The room should have One autoclave(vertical or horizontal)
or cooker with well ventilated exhaust fan.
Also having tools for support while placing the media
bottles in autoclave or cooker for sterilization.
The bottles should sterilized At 1200 C at 15 lb. for 1 hrs.
It contains wrapping paper to wrap and the rubber to tight
the inoculating dishes for sterilization.
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10.  The floor of room covered with tiles to facilitate proper
cleaning. [2],[6],[9]
 Room contains the laminar air flow cabinet with installation
of a HEPA (high efficiency particulate air) filter for filtered
air supply.
 It also requires sodium hypochlorite, mercuric chloride and
70% alcohol for surface sterilization .
 It requires forceps , scalpel, sterile bottles, sterile dishes,
spirit lamp , cotton and hand care etc.
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11. The width of the racks should be 405 mm to 1 m. The distance
between each layer of the racks should be not less then 455
mm. [4],[6],[7]
Photoperiod racks with culture bottles shall be provided. we
mostly operate light intensity with a standard day length of 16
hours and 8 hour dark regime.
Temperature is maintained at a standard 25 + 20 C with 1.5 ton
window A.C. to creating the controlled environment condition.
Light intensity required about 200 lux to 1600 lux for growth
of culture.
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12.  Bottle washing machine and autoclave. [8],[10]
 Freezer and Rotary shaker.
 Multipurpose Magic stove.
 Weighing balance, thermometer .
 Magnetic stirrer and Digital pH meter.
 Laminar air flow with U.V. tube .
 Racks-photoperiodic culture Glass bed sterilizer
 Air conditioner with Stabilizer and Micro air oven etc.
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13.  Glass bottle, Petridis, burette, funnel. [9]
 Beakers and pipettes.
 conical flask with screw cap.
 Graduated measuring cylinder
 Spatula, scalpel and forceps
 Wrapping paper and bucket, gloves etc.
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15. General Chemical Cost
 Glasswares - 10,000-15,000/-
 All chemicals – 20,000/- (Depending)
 Aluminium racks – 5,000/- (Depending on Area)
 Other requirement for laboratories – 25,000/-
Instrumentation
 Water Purifier System – 15,000/-
 Electronic Balance – 1,200/-
 pH Meter – 10,000/-
 Hot Plate – 1,000/-
 Refrigerator – 15,000-25,000/-
 Laminar Air Flow – 35,000/-
 Pipette – 10,000/-
 Autoclave – 30,000/-
 Computer – 18,000/-
 Microscope – 7000/- cntd…
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16. PERSONNEL with needed basic Qualification
 Laboratory In charge (ADMIN)
Bsc.(Bio-tech, Micro-bio) / B.Pharm / B. Tech(Bio-tech)
 Tools Operator
Any Computer certifiedperson(With Experienced in field)
 Laboratory Technician
Bsc(Chem)/D.Pharm
***<N.B. Salary of stuff will be as per requite authority>***
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18.  This PROJECT work helps us to construct an ideal
plant tissue culture laboratory where plantlet
regeneration will takes place from explants by
micro propagation techniques.
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19. 1. Steward F Campion & J. Lohiya Micropropagation,
Ecommons Library Cornell, 1813/18962/2, 2013
2. S Guo-qing & D David , Plant tissue culture In “ The growth
or maintenance of plant cells, tissues, organs or whole
plants in vitro”, CSS/HRT 451, Plant biotechnology Resource
and Outreach Center, MICHIGAN STATE UNIVERSITY, page-
4, 2009
3. “ The growth or maintenance of plant cells, tissues, organs
or whole plants in vitro”, Plant tissue culture by Guo-qing
Song & David Douches, CSS/HRT 451, Plant biotechnology
Resource and Outreach Center, MICHIGAN STATE
UNIVERSITY, page-2, 2009
4. Bhojwani, S.S.:Dantu, P.K, General Requirements and
Techniques “Plant Tissue Culture: An Introductory
Text”.,DOI: 10.1007/ 978-81-322-1025-2 © Springer
India, 2013
5. Singh, S.K.; Srivastava, S. (2006). Plant Tissue Culture.
Campus Book International. ISBN 978-81-8030-123-0
19

20. 6. A practical guide to setting up an IVF lab, Embryo culture
systems and running the unit, Jaypee Brothers Medical
Publishers ISBN 978-93-50905-16-6,1st edition,2013
7. “Plant Tissue Culture: Current Status and Opportunities”
Published by INTECH [open science|open minds], World’s
largest Science, Technology & Medicine open Access book
Publisher, 2013
8. W.C. Evans, Trease and Evans Pharmacognosy, “Plant cell
and tissue culture; biochemical conversions; clonal
propagation” ISBN: 978-0-7020-2617-1 An imprint of Elsevier
WB Saunders Company Limited 1996, Harcourt Publishers
Limited 1999, 2002
9. George, Edwin F.; Hall, Michael A.; De Klerk, Geert-Jan, eds.
(2008). Plant propagation by tissue culture 1. The background
(3rd ed.). Springer.
10. Yadav, R.; Arora, P.; Kumar, D.; Katyal, D.; Dilbaghi, N.;
Chaudhury, A. (2009). "High frequency direct plant
regeneration from leaf, internode, and root segments of Eastern
Cottonwood (Populus deltoides)". Plant Biotechnology
Reports. 3 (3): 175–182. doi:10.1007/s11816-009-0088-5
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