The document discusses the author's research interests in integrating omics approaches like proteomics and metabolomics to discover accurate molecular markers. For the past five years, the author has been using mass spectrometry systems for proteomics and metabolomics and mentoring students in experimental design, sample preparation, data analysis, and publishing findings. Current collaborative projects include investigating glycoproteomes, the effects of honey on aging, proteomes of salivary glands in birds, and proteomes and metabolomes of Alzheimer's mouse brains.
1. Research Statement
Discovery of molecular markers is my favorite. I do believe that the integration of
OMICs approaches can shed more light on the biological facts which help us to find more
accurate molecular markers. Since five years ago, I have been dedicated myself to the integration
of Proteomics and Metabolomics approaches and I would like to continue this journey
considering other OMICs as well. Sine four years ago, I have been using Mass Spectrometry
systems for shotgun Proteomics and Metabolomics and I love the challenges I have been facing.
I would like to contribute in advancing Mass Spectrometry techniques in Multi-OMICs analysis.
In my current position as Specialist in Mass Spectrometry in Medical Biotechnology
Laboratory, I have developed a complete work flow of mass spectrometry-based proteomics and
metabolomics, from sample preparation until publication of the final findings. I am in charge of
Agilent LC/ MS Q-TOF 6550. I am currently collaborating with four professor's PhD and
Master's students. I am mentoring them for proteomics and metabolomics experimental design
and sample preparation. I am in charge of mass spectrometry data acquisition, statistical data
analysis, identification of proteins and metabolites, relative quantitation, gene ontology analysis,
protein-protein network analysis and pathway analysis. I am also contributing in the publication
of the final findings.
I have organized several Mass specrometry workshops with the collaboration of Agilent
Company to train our staff.
The focus of my PhD research was proteomics and metabolomics investigation of cattle
sperm for fertility and heat tolerance selective molecular marker discovery. I am currently
working on the protein molecular markers for sperm sexing.
Few of my current collaborative projects are as follow:
Glycoproteome comparison of whey and milk fat globule membrane components of
human, caprine and bovine milk.
The effect of Glam honey on aging. A proteome investigation of rat heart
mitochondria. (Collaboration with National University of Malaysia)
Proteome comparison of salivary glands from Malaysian Swiftlet breeds.
(Collaboration with University Putra Malayisa, Malaysia)
Proteome and metabolome investigation of the brain from Alzheimer mice model.
(Collaboration with Shiga University, Japan).