The document provides a summary of Ali Ashrafzadeh's background and qualifications. He has over 13 years of experience in molecular research and teaching at veterinary schools. His expertise includes mass spectrometry, proteomics, metabolomics, semen quality evaluation, and experimental design. He has a PhD in Animal Biotechnology and has worked in various roles involving running and maintaining mass spectrometry instruments, collaborating on research projects, mentoring students, and publishing findings.

1. Ali Ashrafzadeh
Green card holder
Corresponding address:
125E 3rd
St, Lansdale, PA, 19446
ali_ash_3000@yahoo.com
https://www.linkedin.com/in/aliashrafzadeh
Summary
Diverse biologist with more than 13 years experiences in molecular research and teaching at the veterinary school.
Expertise in:
 Mass Spectrometry method development and data analysis by different software such as: Masshunter
Qual, MassHunter Quant, Spectrum Mill, Mass Profiler Professional, Scaffold, Blast2GO, STRING and
Peaks.
 Liquid chromatography: Nano-LC, UPLC, reverse phase, normal phase and ion exchange
chromatography method development for 2D LC MS experiments.
 Proteomics (SDS PAGE, IEF, 2D PAGE, In-gel and In-solution tryptic digestion, Sample clean up by
ZipTip, Western Blot, iTRAQ labeled relative quantification, label-free relative quantitation, and LC MS)
 Metabolomics (LC MS/MS Q-TOF, Liquid-Liquid extraction, and Solid phase extraction)
 Semen quality evaluation such as: Sperm Motility test, Sperm DNA Fragmentation test (SDF), Sperm
Morphology test, Reactive Oxygen Species (ROS) evaluation.
 Experimental design and statistical data analysis
 Consultation and Teaching
 Animal husbandry
Experience
LC MS Specialist
2014- 2015 Medical Biotechnology Laboratory, Medical School, University of Malaya, Malaysia
 Maintain and running Agilent UPLC- and LC- MS Q-TOF 6550
 Collaborate with different researchers who are interested in Proteomics and Metabolomics (27 projects)
 Train laboratory staff how to prepare proteomics and metabolomics samples and perform Mass
Spectrometry analysis
 Plan for development of the Mass Spectrometry laboratory
 Develop new collaborations with other institutes
 Mentoring PhD and Master Students
 Proteomics and Metabolomics experimental design
 Mass Spectrometry data acquisition and data analysis of more than 2500 samples
Publication of the final findings
Researcher
2011-2014 Medical Biotechnology Laboratory, Medical School, University of Malaya, Malaysia
 Proteomics and Metabolomics investigation of cattle sperm using Agilent UPLC- and LC- MS Q-TOF
6520.

2. 2010-2011 Agro-Biotechnology Institute (ABI), Malaysia
 Gel-based (SDS PAGE and 2D PAGE) proteomics investigation of cattle sperm.
Adjunct Faculty (Veterinary school, Medical school)
2001-2008 Azad University, Kazeroon branch, Iran
Instructed the following subjects:
 Basics of Animal Nutrition
 Ruminant Ration Formulation
 Animal Genetic
 Animal Breeding
 General Animal Science
 Basic nutrition
Education
2009- 2015 School of Biosciences and Biotechnology, National University of Malaysia (UKM)
 PhD, Animal Biotechnology (Zoology)
2000-2003 College of Agriculture, Shiraz University, Iran
 MS, Animal Science, Animal Physiology
1995-2000 College of Agriculture, Shiraz University, Iran
 BSc, Animal Science, Graduated top student (first honored)
Conference Presentations
 Sperm Sexing (2006). Presented in the Scientific Seminar on “Usage of Biotechnology Sciences in
Animals” in Azad University, Kazeroun Branch. (Kazeroun, Iran)
 Effective Factors in Dairy Cows Fertility (2006). Presented in a Workshop Held in Fars Live Stock Industry
Corporation. (Shiraz, Iran)
 Sperm Sexing and Dairy Cattle Breeding (2007). Presented in a Workshop in Fars Live Stock Industry
Corporation. (Shiraz, Iran)
 Proteome Comparison of Sexed and Non-sexed Bovine Sperm for Novel Molecular Marker Discovery
(2010). Presented in the Third Semester PhD Student Colloquium. (Kuala Lumpur, Malaysia)
 Sperm Fertility Related Proteins (2011). Presented in the First Iranian Students Scientific Conference in
Malaysia. (Kuala Lumpur, Malaysia)
 Sperm Fertility Related Homolog Proteins in Bos taurus (2011). Presented in the 36th
Annual Conference
of Malaysian Society of Biochemistry and Molecular Biology (MSBMB). (Kuala Lumpur, Malaysia)
 Proteomic Comparison of Kedah Kelantan (Bos indicus) and Mafriwal (Bos taurus × Bos indicus) Sperm
for Fertility Molecular Marker Discovery (2011). Presented in the Second Word Congress of Biotechnology.
(Philadelphia, USA)
 Shotgun Proteomic Comparison of Kedah Kelantan (Bos indicus) and Mafriwal (Bos taurus × Bos indicus)
Sperm for Discovery of Fertility Molecular Marker (2012). Presented in the 37th
Annual Conference of
Malaysian Society of Biochemistry and Molecular Biology (MSBMB). (Kuala Lumpur, Malaysia)

3.  Non-Labeled Relative Comparison of Kedah Kelantan (Bos indicus) and Mafriwal (Bos taurus × Bos
indicus) Sperm Proteins for Fertility Molecular Marker Discovery (2012). Presented in the 4th
Australasian
Metabolomic Symposium. (Puncak Alam, Malaysia). Awarded as the best poster presentation.
 Integration of Metabolomics and Proteomics for Fertility Molecular Marker Discovery: Unraveling
Differences in Energy Metabolism Between Fertile and Sub-Fertile Bull Sperm (2012). Presented in the 1st
Malaysian Proteomics Conference. (Penang, Malaysia)
 More Efficient Carbohydrate Metabolism Accounts for Higher Fertility of Zebu Cattle (Bos Indicus) in
Tropical Areas (2013). Presented in the 2nd Asian Regional Conference on Systems Biology.(Bangi,
Malaysia)
 Membrane Proteins Associated with Sperm-Oocyte Interaction: A Proteomic Comparison between
Kedah Kelantan (Bos indicus) and Mafriwal (Bos taurus × Bos indicus) Sperm (2013). Presented in
The 13th
Postgraduate Colloquium, Faculty of Science and Technology. (National University of
Malaysia)
 LC MS Q-TOF: Shotgun Proteomics and Metabolomics Approaches (2015). Presented as an invited
speaker in Medical Biotechnology Laboratory Open Day, Faculty of Medicine. (University of Malaya,
Malaysia)
 Proteomics and Metabolomics-based Approaches in Fertility Molecular Marker Discovery (2015).
Presented as an invited speaker in Agilent Conference, Kuala Lumpur, Malaysia.
Articles in Academic Journals
 Ashrafzadeh, A., Karsani, S.A. & Nathan, S. 2013. Mammalian sperm fertility related proteins.
International journal of medical sciences 10 (12): 1649.
 Ashrafzadeh, A., Nathan, S. & Karsani, S. 2013. Comparative analysis of Mafriwal (Bos taurus × Bos
indicus) and Kedah Kelantan (Bos indicus) sperm proteome identifies sperm proteins potentially responsible
for higher fertility in a tropical climate. International Journal of Molecular Sciences 14 (8): 15860-15877.
 Ashrafzadeh, A., Nathan, S., Othman, I., Yee, T.T. & Karsani, S.A. 2013. Membrane proteins associated
with sperm-oocyte interaction: A proteomic comparison between Kedah Kelantan (Bos indicus) and
Mafriwal (Bos taurus × Bos indicus) sperm. AIP Conference Proceedings 1571 (1): 180-183.
 Thio, C.L.-P., Yusof, R., Ashrafzadeh, A., Bahari, S., Abdul-Rahman, P.S. & Karsani, S.A. 2015.
Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus. PLoS ONE 10
(6): e0129033.
 Lee, C.-S., Taib, N.A.M., Ashrafzadeh, A., Fadzli, F., Harun, F., Rahmat, K., Hoong, S.M., Abdul-Rahman,
P.S. & Hashim, O.H. 2016. Unmasking Heavily O-Glycosylated Serum Proteins Using Perchloric Acid:
Identification of Serum Proteoglycan 4 and Protease C1 Inhibitor as Molecular Indicators for Screening of
Breast Cancer. PLoS ONE 11 (2): e0149551.
Workshops Organizer
 Control of Shrimp White Spot Disease (2005). Scientific workshop organizer (Bushehr, Iran)
 Food Management in Shrimp Farms (2006). Scientific workshop organizer (Bushehr, Iran)
 Hygiene & Diseases of Farmed Shrimps (2006). Scientific workshop organizer (Bushehr, Iran)
 Management of Water & Shrimp Pond Preparation (2006). ). Scientific workshop organizer (Bushehr, Iran)
Certifications
 Quantitative Mass Spectrometry in Multi-OMICs analysis
 Agilent 6500 series Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry
 Agilent 6500 series Q-TOF Techniques and Operation
 Design and Optimization of Q-TOF Acquisition and Qual Methods (Bio-Molecules/Peptides)
 Design and Optimization of Q-TOF Acquisition and Qual Methods (Bio-Molecules/Metabolites)

4.  Proteomics in Agro-biotechnology
 The Essential Role of Two Dimensional Electrophoresis- Mass Spectrometry (2DE-MS) in Proteomics
 Applied Biosystems LCMS/MS
 Applied Biosystems iTRAQ Labeling Technology
 StepOnePlusTM
Real-Time PCR System Operational Training
 National Advanced Reproductive Biotechnology
 Statistics & System Thinking as Applied in Agro-Biotechnology
 Manuscript Writing for Journal
 Publication Writing
 The Effective Use of Research Tools and Resources
 Writing and Publishing in ISI Journals
Awards
 Mass spectrometry and SWATH analysis champion award
 Best poster presentation award in “4th
Australasian Metabolomics conference”
Current collaborative projects
 Glycoproteome comparison of whey and milk fat globule membrane components of human,
caprine and bovine milk.
 The effect of Glam honey on aging. A proteome investigation of rat heart mitochondria.
(Collaboration with National University of Malaysia)
 Proteome comparison of salivary glands from Malaysian Swiftlet breeds. (Collaboration with
University Putra Malayisa, Malaysia)
 Proteome and metabolome investigation of the brain from Alzheimer mice model. (Collaboration
with Shiga University, Japan).
Knowledgeable about computer software as follow
 MassHunter
 MassHunter Qual
 Spectrum Mill
 Blast2GO
 Mass Profiler Professional
 PEAKs
 STRING
 Scaffold
 Excel
 Power Point
 Word

5. Cover Letter
Dear Sir/Madam,
I am very interested in joining your well-known institute. I have a PhD in Animal Biotechnology
and I am a holder of permanent residency status in the United States.
Aside from my educational and research background, I have more than six years of experience in
college level teaching which included courses such as genetics, animal breeding and nutrition to the
veterinary college students. In addition, I am well versed running and maintaining Agilent UPLC-
and nano-LC MS Q-TOF. I have also experience running other mass spectrometry systems (e.g. AB
Sciex (MALDI TOF) and Thermo Fisher Scientific (Orbitrap)). I have been working as Mass
Spectrometry manager running Agilent UPLC and LC MS Q-TOF 6550.
Using proteomics and metabolomics approaches; I have conducted intensive research
throughout past several years. Based on my research, I have five years of successful experience in
working with different proteomics platforms such as: SDS PAGE, Two Dimensional Gel
Electrophoresis (2D PAGE), MALDI-TOF Mass Spectrometry, Liquid Chromatography Mass
Spectrometry (LC MS/MS), and isotope labeling and non-labeling relative quantitation.
I am well versed in using different proteomics software for data acquisition (MassHunter),
qualitative data analysis (MassHunter Qual), protein identification (Spectrum Mill), Gene Ontology
analysis (Blast2GO and scaffold), non-labeling relative quantitation (Mass Profiler Professional),
absolute quantification of proteins (MassHunter Quant), pathway analysis (Mass Profiler
Professional and KEGG data base), and protein-protein interaction (STRING). As integrative biology
is the most attractive approach in the modern molecular biology studies, I have used the LC MS/MS for
metabolomics investigations. Furthermore, I have used different databases such as: Human Metabolome,
Lipid maps and KEGG on regular basis as well as being familiar with RT-PCR and DNA fragmentation
test.
Additionally, I am familiar with grant proposal writing since I have prepared a grant proposal for
my research and have been able to successfully secure funds from the Ministry of Science Technology
and Innovation of Malaysia.
I am enclosing my resume for your consideration. As I found your institute as one of the
advanced institute in the field of metabolomics and proteomics, I look forward to hear from you about
any opportunity in your research team.
Sincerely,
Ali Ashrafzadeh