A functional genomics experiment is carried out in E coli t.pdf
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A functional genomics experiment is carried out in E. coli to examine global levels of gene expression in various types of minimal growth medium. RNA extracted from the experimental culture is labeled with a molecule that fluoresces red, and RNA extracted from the control culture is labeled with a molecule that fluoresces green. The experimental and control samples are mixed prior to hybridization. Shown here are spots on the microarray corresponding to five genes: trpE (the first gene in the tryptophan biosynthetic operon), lacI, lacZ, lacY, and crp (which encodes the cAMP receptor protein). Color the spots red, green, or yellow according to the relative levels of expression of each gene in the experimental and control cultures. (Hint: Before answering, think carefully about how the cAMP receptor protein co-regulates the lac operon.).
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presumably because it provides maximal conservation of energy for the cell. We can often
observe the consequence of differential transcription due to the presence or absence of particular
proteins or the growth in particular environments. Control can also occur at translation; the
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context. Thus, protein production is carefully regulated by multiple mechanisms that modulate
both transcriptional and translational pathways. Control of transcription initiation by RNA
polymerase is a predominant mechanism for regulating expression of specific proteins,
presumably because it provides maximal conservation of energy for the cell. We can often
observe the consequence of differential transcription due to the presence or absence of particular
proteins or the growth in particular environments. Control can also occur at translation; the
mRNA is synthesized, but only in certain circumstances is it translated. Control can also occur at
the level of protein function; the protein is inactive, or activity is not observed due to the lack of
the substrate. In this lab we will observe differential expression of two different genes encoded
on plasmids. We will analyze transcriptional activity, translational activity, and protein function.
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plasmid and pass it to daughter cells (vertical transmission) and to neighboring cells (horizontal).
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copy number. 2. Lab generated plasmids typically also contain a selectable marker (antibiotic
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pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
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specific constellation of proteins required for optimal function and growth varies with cellular
age and environmental context. Thus, protein production is carefully regulated by multiple
mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
expression of specific proteins, presumably because it provides maximal conservation of energy
for the cell. We can often observe the consequence of differential transcription due to the
presence or absence of particular proteins or the growth in particular environments. Control can
also occur at translation; the mRNA is synthesized, but only in certain circumstances is it
translated. Control can also occur at the level of protein function; the protein is inactive, or
activity is not observed due to the lack of the substrate. In this lab we will observe differential
expression of two different genes encoded on plasmids. We will analyze transcriptional activity,
translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
have plasmids and will replicate the plasmid and pass it to daughter cells (vertical transmission)
and to neighboring cells (horizontal). Plasmids are a mechanism of gene diversity. In order to
stably retain the plasmid, there needs to be some type of metabolic reason for the bacteria to
maintain the plasmid. In other words, the plasmid confers an advantage. Plasmids contain: 1. Ori:
the plasmid may present is low or high copy number. 2. Lab generated plasmids typically also
contain a selectable marker (antibiotic resistance), 3. Additional gene for ease of visual screening
4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
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BACKGROUND CONTEXT Lab: Differential Expression Differential gene expression provides
the ability for a cell or organism to respond to a constantly changing external environment. The
specific constellation of proteins required for optimal function and growth varies with cellular
age and environmental context. Thus, protein production is carefully regulated by multiple
mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
expression of specific proteins, presumably because it provides maximal conservation of energy
for the cell. We can often observe the consequence of differential transcription due to the
presence or absence of particular proteins or the growth in particular environments. Control can
also occur at translation; the mRNA is synthesized, but only in certain circumstances is it
translated. Control can also occur at the level of protein function; the protein is inactive, or
activity is not observed due to the lack of the substrate. In this lab we will observe differential
expression of two different genes encoded on plasmids. We will analyze transcriptional activity,
translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
have plasmids and will replicate the plasmid and pass it to daughter cells (vertical transmission)
and to neighboring cells (horizontal). Plasmids are a mechanism of gene diversity. In order to
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maintain the plasmid. In other words, the plasmid confers an advantage. Plasmids contain: 1. Ori:
the plasmid may present is low or high copy number. 2. Lab generated plasmids typically also
contain a selectable marker (antibiotic resistance), 3. Additional gene for ease of visual screening
4. Multiple cloning site
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Gene regulation is the process used to control the timing, location and amount in which genes are expressed. The process can be complicated and is carried out by a variety of mechanisms, including through regulatory proteins and chemical modification of DNA.
Mod 4 regulation of gene expression -notes SH.pdf
This note is created for BSc students of molecular biology, biochemistry, biotechnology microbiology, and other biological science students.
1 At least 2 questions from this section will be on the .docx
1
At least 2 questions from this section will be on the final exam
SAMPLE QUESTIONS FOR THE FINAL EXAM
Question 1. Ferritin is a protein involved in the storage of iron inside cells. To prevent toxic accumulation of
too much iron inside cells, the intracellular level of ferritin is tightly regulated. To study the regulation of
ferritin synthesis, mammalian cells are grown with or without iron in the culture medium. Note that iron in the
culture medium is rapidly transported inside cells.
a) Upon addition of iron to the culture medium, the intracellular concentration of ferritin mRNA is unchanged
but the concentration of ferritin protein increases. How do you think ferritin expression is regulated? Briefly
explain.
The regulatory sequence given below is found in the ferritin mRNA between the cap structure and the start
codon.
5’-GGGUUUCCGUUCAACAGUGCUUGGACGGAAACCC-3’
Mutations within in this sequence are used to study the regulation of ferritin expression. The following
observation are made:
• ferritin expression is high, independent of the iron concentration, when (i) the entire region is deleted, or
(ii) the region located upstream of the underlined sequence is deleted or (iii) the underlined sequence is
replaced with a random sequence.
• ferritin expression remains iron-dependent when this region is replaced by the following sequence:
5’-GGGCUCAGGUUCAACAGUGCUUGGACCUGAGCCC-3’.
Note that the sequence differences are indicated in bold.
b) Explain why these observations suggest that both sequence and structure of the 5’ end of ferritin mRNA are
important for the regulation of ferritin expression.
c) Ferritin translation becomes iron-independent when the regulatory sequence is moved from the 5’ side
(upstream of the open reading frame) to the 3’ side (downstream of the open reading frame) of ferritin mRNA.
Which step of ferritin translation do you think is affected by the intracellular level of iron?
d) IRP is a protein involved in the regulation of ferritin expression. Anti-IRP antibodies attached to sepharose
beads are added to a cell extract, then the extract is centrifuged to separate the pellet fraction (containing the
sepharose beads ) from the supernatant fraction.
If the cells are cultured in the absence of iron, ferritin mRNA is found together with IRP in the pellet. In
contrast when cells are cultured in the presence of iron ferritin mRNA remains in the supernatant fraction while
IRP alone is found in the pellet. Briefly explain the likely role of IRP in the regulation of ferritin expression.
Question 2. You are studying the development of a newly discovered insect. Like drosophila, it undergoes a
stage in early larval development where the eve gene is expressed in a pattern of 7 stripes. You are particularly
interested in stripes 2 and 5. The following figures show the organization of the cis-acting elements that control
the expression o ...
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Current Attempt in Progress Sexpili are to conjugation as is/are to transduction. bacteriophages
plasmids genes the host chromosome
The anticodon of tRNA interacts with the
codon on mRNA. amino acid. backbone on rRNA. ATP for reaction energetics
A microbiologist designs an experiment to transfer a gene from a pathogenic bacterium into a
laboratory strain of Escherichia colit discover whether the gene confers virulence. One method
for transferring the gene into E. coli would be to use a ligase reaction. restriction digestion.
polymerase chain reaction. cloning vector. sequencing reaction.
x Your answer is incorrect. What sites do eukaryotes have for gene regulation that are not found
in prokaryotes? Select all that apply. Nucleus and cytoplasm Operons Poly(A) talis Ribosomes
Histones
When E. coli in added to a medium with lactose and no glucose, what happens to the repressor
system of the lac operon? 1. The lac repressor is inactivated 2. Transcription of the lac operon
genes into a single mRNA 3. -galactosidase and lac permease are synthesized 4. Lactose is
rapidly metabolized by enzymes 1, 2,3 and 4 2, 3 and 4 1,3 and 4 1,2 and 3
Which of the following are found as part of an operon? Select all that apply. Structural genes
operator promoter intron ribosome
DNA (Select all that apply) is a double stranded molecule. is used as a template to synthesize
proteins. contains the hereditary information of a cell. contains all bases A, T, C and G..
A ince barsakta ya emlsifikasyon sreci B dklamay dzenl.pdf
(a) Find a point estimate for the average amount lost after one week on this diet. Is this an
unbiased estimate of the population mean? Explain. Yes, this an unbiased estimate of the
population mean, since E(x)=. Yes, this an unbiased estimate of the population mean, sinceE(x)=
14. No, this is not an unblased estimate of the population mean, since E(x)=. No, this is not an
unbiased estimate of the population mean, since the sample size is too small. No, this is not an
unbiased estimate of the population mean, since E(x)=. (b) Find a point estimate for the variance
of the amount lost on this diet, (Round your answer to four decimal places.)(a) Find a point
estimate for the average amount lost after one week on this diet. Is this an unbiased estimate of
the population mean? Explain. Yes, this an unbiased estimate of the population mean, since E(x)=
. Yes, this an unbiased estimate of the population mean, since E(x)=. No, this is not an unbiased
estimate of the population mean, since E(x)=. No, this is not an unbiased estimate of the
population mean, since the sample size is too small. No, this is not an unbiased estimate of the
population mean, since E(x)=. (b) Find a point estimate for the variance of the amount lost on this
diet. (Round your answer to four decimal places.) Is this an unbiased estimate of the population
variance? Explain. Yes, this an unblased estimate of the population variance, since E(s2)=2. Yes,
this an unbiased estimate of the population variance, since E(s2)=2. No, this is not an unbiased
estimate of the population variance, since E(s2)=2. No, this is not an unbiased estimate of the
population variance, since the sample size is too small. No, this is not an unbiased estimate of the
population variance, since E(s2)=a2. (c) Find a point estimate for the standard deviation of the
amount lost on this diet. (Round your answer to three decimal places.).
A is a nonempty set and G is a finite group that can be see.pdf
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48851987969416198779 (a) Min= (Simplify your answer.) Q1= (Simplify your answer. Do not
round.) Q2= (Simplify your answer. Do not round.) Q3= (Simplify your answer. Do not round.) Max
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specific constellation of proteins required for optimal function and growth varies with cellular
age and environmental context. Thus, protein production is carefully regulated by multiple
mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
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translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
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conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
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been introduced, can be easily distinguished from the non-recombinants based on color
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mechanisms that modulate both transcriptional and translational pathways. Control of
transcription initiation by RNA polymerase is a predominant mechanism for regulating
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for the cell. We can often observe the consequence of differential transcription due to the
presence or absence of particular proteins or the growth in particular environments. Control can
also occur at translation; the mRNA is synthesized, but only in certain circumstances is it
translated. Control can also occur at the level of protein function; the protein is inactive, or
activity is not observed due to the lack of the substrate. In this lab we will observe differential
expression of two different genes encoded on plasmids. We will analyze transcriptional activity,
translational activity, and protein function. Plasmids are extra-chromosomal DNA. Bacteria often
have plasmids and will replicate the plasmid and pass it to daughter cells (vertical transmission)
and to neighboring cells (horizontal). Plasmids are a mechanism of gene diversity. In order to
stably retain the plasmid, there needs to be some type of metabolic reason for the bacteria to
maintain the plasmid. In other words, the plasmid confers an advantage. Plasmids contain: 1. Ori:
the plasmid may present is low or high copy number. 2. Lab generated plasmids typically also
contain a selectable marker (antibiotic resistance), 3. Additional gene for ease of visual screening
4. Multiple cloning site
pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers.
The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the
abbreviation for the University of California, where early work on the plasmid series had been
conducted. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the
most widely used vector molecules as the recombinants, or the cells into which foreign DNA has
been introduced, can be easily distinguished from the non-recombinants based on color
differences of colonies on growth media. pUC18 is similar to pUC19, but the MCS region is
reversed. - pUC 19 has an origin of replication and is maintained at a high copy number. -
pUC19 encodes for an ampicillin resistance gene (amopR), via a -lactamase enzyme that
functions by degrading ampicillin and reducing its toxicity to the host. - It has an N-terminal
fragment of -galactosidase (lacZ) gene of E. coli which allows for visual screening of
recombinant.
Gene Regulation.pptx
Gene regulation is the process used to control the timing, location and amount in which genes are expressed. The process can be complicated and is carried out by a variety of mechanisms, including through regulatory proteins and chemical modification of DNA.
Mod 4 regulation of gene expression -notes SH.pdf
This note is created for BSc students of molecular biology, biochemistry, biotechnology microbiology, and other biological science students.
1 At least 2 questions from this section will be on the .docx
1
At least 2 questions from this section will be on the final exam
SAMPLE QUESTIONS FOR THE FINAL EXAM
Question 1. Ferritin is a protein involved in the storage of iron inside cells. To prevent toxic accumulation of
too much iron inside cells, the intracellular level of ferritin is tightly regulated. To study the regulation of
ferritin synthesis, mammalian cells are grown with or without iron in the culture medium. Note that iron in the
culture medium is rapidly transported inside cells.
a) Upon addition of iron to the culture medium, the intracellular concentration of ferritin mRNA is unchanged
but the concentration of ferritin protein increases. How do you think ferritin expression is regulated? Briefly
explain.
The regulatory sequence given below is found in the ferritin mRNA between the cap structure and the start
codon.
5’-GGGUUUCCGUUCAACAGUGCUUGGACGGAAACCC-3’
Mutations within in this sequence are used to study the regulation of ferritin expression. The following
observation are made:
• ferritin expression is high, independent of the iron concentration, when (i) the entire region is deleted, or
(ii) the region located upstream of the underlined sequence is deleted or (iii) the underlined sequence is
replaced with a random sequence.
• ferritin expression remains iron-dependent when this region is replaced by the following sequence:
5’-GGGCUCAGGUUCAACAGUGCUUGGACCUGAGCCC-3’.
Note that the sequence differences are indicated in bold.
b) Explain why these observations suggest that both sequence and structure of the 5’ end of ferritin mRNA are
important for the regulation of ferritin expression.
c) Ferritin translation becomes iron-independent when the regulatory sequence is moved from the 5’ side
(upstream of the open reading frame) to the 3’ side (downstream of the open reading frame) of ferritin mRNA.
Which step of ferritin translation do you think is affected by the intracellular level of iron?
d) IRP is a protein involved in the regulation of ferritin expression. Anti-IRP antibodies attached to sepharose
beads are added to a cell extract, then the extract is centrifuged to separate the pellet fraction (containing the
sepharose beads ) from the supernatant fraction.
If the cells are cultured in the absence of iron, ferritin mRNA is found together with IRP in the pellet. In
contrast when cells are cultured in the presence of iron ferritin mRNA remains in the supernatant fraction while
IRP alone is found in the pellet. Briefly explain the likely role of IRP in the regulation of ferritin expression.
Question 2. You are studying the development of a newly discovered insect. Like drosophila, it undergoes a
stage in early larval development where the eve gene is expressed in a pattern of 7 stripes. You are particularly
interested in stripes 2 and 5. The following figures show the organization of the cis-acting elements that control
the expression o ...
Attenuation in Tryptophan Operon_Mamun_Neel.pptx
Tryptophan Operon Attentuation presentaton first year college level.
Rna editing as a drug target identification of inhibitors of rel 1 bsp 210
Low through put radiomimetic assay to identify putative inhibitors of the Tryp enzyme REL 1 'in vitro' predicted by 'in silico' modelling
R Gene Expression and Transcription Profiling
R Gene expression and transcription profiling in plants. Incorporating gene resistance and its transcription database.
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
Current Attempt in Progress Sexpili are to conjugation as is/are to transduction. bacteriophages
plasmids genes the host chromosome
The anticodon of tRNA interacts with the
codon on mRNA. amino acid. backbone on rRNA. ATP for reaction energetics
A microbiologist designs an experiment to transfer a gene from a pathogenic bacterium into a
laboratory strain of Escherichia colit discover whether the gene confers virulence. One method
for transferring the gene into E. coli would be to use a ligase reaction. restriction digestion.
polymerase chain reaction. cloning vector. sequencing reaction.
x Your answer is incorrect. What sites do eukaryotes have for gene regulation that are not found
in prokaryotes? Select all that apply. Nucleus and cytoplasm Operons Poly(A) talis Ribosomes
Histones
When E. coli in added to a medium with lactose and no glucose, what happens to the repressor
system of the lac operon? 1. The lac repressor is inactivated 2. Transcription of the lac operon
genes into a single mRNA 3. -galactosidase and lac permease are synthesized 4. Lactose is
rapidly metabolized by enzymes 1, 2,3 and 4 2, 3 and 4 1,3 and 4 1,2 and 3
Which of the following are found as part of an operon? Select all that apply. Structural genes
operator promoter intron ribosome
DNA (Select all that apply) is a double stranded molecule. is used as a template to synthesize
proteins. contains the hereditary information of a cell. contains all bases A, T, C and G..
Similar to A functional genomics experiment is carried out in E coli t.pdf (20)
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE B.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE B.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE BAC.pdf
ONLY THE LAST QUESTION IS THE POINT OF POST. THE OTHER PAGES ARE BAC.pdf
1 At least 2 questions from this section will be on the .docx
1 At least 2 questions from this section will be on the .docx
Rna editing as a drug target identification of inhibitors of rel 1 bsp 210
Rna editing as a drug target identification of inhibitors of rel 1 bsp 210
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
Current Attempt in Progress Sexpili are to conjugation as isare to t.pdf
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A ince barsakta ya emlsifikasyon sreci B dklamay dzenl.pdf
(a) Find a point estimate for the average amount lost after one week on this diet. Is this an
unbiased estimate of the population mean? Explain. Yes, this an unbiased estimate of the
population mean, since E(x)=. Yes, this an unbiased estimate of the population mean, sinceE(x)=
14. No, this is not an unblased estimate of the population mean, since E(x)=. No, this is not an
unbiased estimate of the population mean, since the sample size is too small. No, this is not an
unbiased estimate of the population mean, since E(x)=. (b) Find a point estimate for the variance
of the amount lost on this diet, (Round your answer to four decimal places.)(a) Find a point
estimate for the average amount lost after one week on this diet. Is this an unbiased estimate of
the population mean? Explain. Yes, this an unbiased estimate of the population mean, since E(x)=
. Yes, this an unbiased estimate of the population mean, since E(x)=. No, this is not an unbiased
estimate of the population mean, since E(x)=. No, this is not an unbiased estimate of the
population mean, since the sample size is too small. No, this is not an unbiased estimate of the
population mean, since E(x)=. (b) Find a point estimate for the variance of the amount lost on this
diet. (Round your answer to four decimal places.) Is this an unbiased estimate of the population
variance? Explain. Yes, this an unblased estimate of the population variance, since E(s2)=2. Yes,
this an unbiased estimate of the population variance, since E(s2)=2. No, this is not an unbiased
estimate of the population variance, since E(s2)=2. No, this is not an unbiased estimate of the
population variance, since the sample size is too small. No, this is not an unbiased estimate of the
population variance, since E(s2)=a2. (c) Find a point estimate for the standard deviation of the
amount lost on this diet. (Round your answer to three decimal places.).
A is a nonempty set and G is a finite group that can be see.pdf
(a) Find the five-number summary, and (b) draw a box-and-whisker plot of the data.
48851987969416198779 (a) Min= (Simplify your answer.) Q1= (Simplify your answer. Do not
round.) Q2= (Simplify your answer. Do not round.) Q3= (Simplify your answer. Do not round.) Max
= (Simplify your answer.)(b) Choose the correct box-and whisker plot below. A. B. c. D..
a In a punctuated module new species change most as they .pdf
A financial analyst is using the capital asset pricing model (CAPM) to find the expected annual
retum on stocks. The analyst assumes: Risk-free rate, R4=3% Market risk premiam, [E(RM)R1]=
8% If the analyst were to plot the CAPM to illustrate the seourity maset ine (SM.) what is the
mumerical value of the slope of the SML? 5 6 3 11.
A Imagine you are a communication consultant giving advice .pdf
A financial advisor wants to determine how the amount of time spent on financial education affects
the amount of debt. The equation of the regression line is Y^=50000+(500)X A financial education
time of 36 hours is found to result in an amount of debt of 32000 dollars. What is the predicted
amount of debt? dollars Where is the observed value in relation to the regression line? rinancial
eaucation time (in nours) A financial education time of 30 hours is found to result in a amount of
debt of 38134 dollars. What is the predicted amount of debt? dollars Where is the observed value
in relation to the regression line? A financial education time of 39 hours is found to result in a
amount of debt of 29931 dollars. What is the predicted amount of debt? dollars Where is the
observed value in relation to the regression line?.
A group of college DJs surveyed students to find out what mu.pdf
A group of college DJs surveyed students to find out what music to plan for their upcoming parties.
Thirty percent of the students preferred dubstep, 25% of the students liked trance music, and 20%
wanted to hear only house music. Fifteen percent of the respondents selected both dubstep and
trance. The proportion of students that like neither trance music nor dubstep is a. 0% b. 20%. c.
40%. d. 60%. ype a, b, c, d _._.
A health system has forecast net patient revenue in the firs.pdf
A health system has forecast net patient revenue in the first 3 months of the year as follows
(figures in millions): January, $200; February, $140; March, $200; 70% of services are usually paid
for in the month that they take place, 20% in the following month, and the final 10% in the next
month. Receivables at the end of December were $100 million. What is the forecast for
outstanding receivables at the end of March?.
A gene which is 1440 bp long codes for polypeptide that is 1.pdf
A gene which is 1440 bp long codes for polypeptide that is 192 amino acids long. Please explain
the discrepancy
The gene contains non coding regions called introns
There are 600bp at the end of the gene (after the stop codon) which comprise the untranslated
region
There is no discrepancy, the genetic code is 6 letters long.
The promoter and untranslated regions at the 3' end of the gene comprise the remaining 864bp..
a Identificar y discutir los factores bsicos de comunicaci.pdf
A farmer who only sells produce to local residents in his town wishes to expand to a neighboring
city. The farmer calls everyone in the phone book with a last name starting with "S" in the
neighboring city and asks them if they would be interested in buying the produce. 242 told the
farmer that they would be interested, 576 said they would not be interested and 138 refused to
answer. Which statement is true?.
A Health Behaviour in Schoolaged Children study found that .pdf
A Health Behaviour in School-aged Children study found that 7% of adolescents were classified as
problematic social media users. We take a random sample of 10 adolescents. (a) Calculate the
probability that at least one of them is a problematic social media user. four decimal places)
(please round to (b) Calculate the probability that exactly 2 of them are problematic social media
users. four decimal places) (please round to (c) Calculate the probability that at most 1 of them is.
a Identify all NE throughout 3 you are only required to p.pdf
A fast food drive-through restaurant has a single serving booth staffed by a single operative. There
is space for ten vehicles to wait if the service booth is occupied. If all ten spaces are occupied
when a car arrives, it has to drive past and the customer is lost. Cars arrive at random at a rate of
55 per hour. Service times are exponential with a mean of one minute.
Employees are paid 10.30 per hour. Time waiting before receiving service has a high value in the
fast food industry. Here it is valued at 95 per hour. The average revenue per customer is 15.
For this queueing situation, use the queueing template spreadsheet (and enter answers to the
accuracy displayed by the spreadsheet) to calculate the average number of cars at the drive-
through (the whole system) and the average time a car is waiting to be able to drive up to the
booth and make their order and the probability a customer will fail to achieve entry to the drive-
through . Note: Fill the first three blanks with 4 decimal places.
Calculate the hourly cost of the system under present circumstances . Note: Enter this and all
further costs in this question to 2 decimal places.
The manager of the drive-through restaurant could choose to build and staff extra serving booths if
it resulted in an overall cost saving. However, the additional space required would mean a
reduction in the space for cars to wait for service for each additional booth, two waiting spaces
are lost.
To minimise costs, additional booths should be built. The waiting space is reduced to . This
achieves a minimum hourly cost of.
A hoverboard is a levitating board similar in shape and size.pdf
A factory has several departments. A department may have many employees but must have at
least seven. Every employee works for one and only one department. Every department has a
manager -- only one manager per department. A manager is an employee of the company, but not
all employees are managers. A department may have many machines, and every machine is
assigned to a specific department. Products are produced on machines. A product can be an
assembly of several different components or a single piece. Every product goes through one or
more machines for appropriate production operations. Likewise, several products may go through
a particular machine for a production operation. The employee has a first name, last name, and
middle name, gender, address, and salary. An employee number uniquely identifies an employee.
Departments have departments name, type, and location. The department's name and number
are both unique identifier of a department. Every machine will have a unique machine number,
name, type, and vendor's name.
When a machine goes for maintenance, the date of maintenance must be captured, since a
maintenance activity is identified by the date of the maintenance for each machine. The attributes
of maintenance activity are time taken and cost.
A product is identified by its component ID, component name, and description.
7- Create an ERD by first identifying the entities, then their relationships, and finally add the
attributes to each entity.
8- Create a logical data model from ERD, including primary and foreign keys.
9- Implement the logical model into a DDL and build the database in SQL Server (or any other
DBMS that you have installed)
10- Create some synthetic data and insert the data into the database.
PreviousNext.
A hospital is trying to cut down on emergency room wait time.pdf
a) Explain the main characteristics of the normal distribution. (4 marks) Terangkan ciri-ciri utama
taburan normal. b) The volume of sales of a company have a mean of 20,000 units per month with
a standard deviation of 3,000 units per month. You may assume that sales are normally
distributed. Find the probability that the volume of sales per month will be: Jumlah jualan syarikat
yang mempunyai min sebanyak 20,000 unit sebulan dengan sisihan piawai 3,000 unit sebulan.
Anda boleh menganggap bahawa jualan adalah bertaburan normal. Cari kebarangkalian bahawa
jumlah jualan bagi setiap bulan adalah: i) less than 15,000 kurang daripada 15,000 ii) between
15,300 and 23,000 antara 15,300 dan 23,000 iii) over 23,000 melebihi 23,000 (8 marks) c) The
accountants report that for sales of 23,000 or over, the profit generated is RM25,000; for sales
between 15,300 and 23,000, the profit generated is RM8,000 and for sales less than 15,000, there
is a loss of RM20,000. Calculate the expected net profit..
A healthy female Labrador retriever was mated with two healt.pdf
A healthy female Labrador retriever was mated with two healthy males. In the two litters produced
some of the offspring had hemophilia. If the female is bred to one of her male offspring that does
not have hemophilia, then the probability of the female offspring of this crossing having hemophilia
is:
elect one:
a.0%
b.25%
c.75%
d.100%.
A hypertonic solution is prepared by the addition of sucrose.pdf
A family with an interesting distribution of Leigh syndrome symptoms [loss of motor function,
(Ataxia; A); with peripheral neuropathy (PN)]. a) Construct a pedigree b) Explain the mode of
inheritance c) Why is there so much variation in symptoms? d) In what way does this condition
differ from classical albinism in terms of expression and transition?.
A group of n people were removed from the hotel After a mon.pdf
A group of n people were removed from the hotel. After a month, the kidnappers need to get rid of
these people, but they just don't want to let them go. They inform the hostages one evening that
the next morning they will be lined up one behind the other so that everyone sees those in front of
them but none behind. Then the captors will pass, starting with the last in line, and put a hat on
everyone's head, either red or black. Hats put down, the kidnappers will come back, always
starting at the end of the line, asking the simple question: what color hat do you have?. If the
person gives the correct color, they will be released. Otherwise, it will be executed instantly. The
only answer allowed (one that will not cause the responder to execute) is the color, black or red.
(a) How many people can be saved and how? Explain in detail (a formal proof but not necessary
at this time).
(b) Give (draw) an AFD that models the rescue protocol, with its interpretation. Pay attention to the
alphabet and the states.
2. Give a context-free grammar that generates the language L = {w {(,)} : w is parenthesis}. Prove
your answer..
A group of employees of Unique Services will be surveyed abo.pdf
A group of employees of Unique Services will be surveyed about a new pension plan. In-depth
interviews with each employee selected in the sample will be conducted. The employees are
classified as follows: What is the probability that the first person selected is either in management
or in supervision? Select one: a. 0 b. 0.21 c. 0.15 d. 0.06.
A Genler aras sekanslar insan genomunun gt60n oluturur.pdf
A. Genler aras sekanslar, insan genomunun >%60'n oluturur. Bu genler aras diziler nereden
geliyor?
B. levlerinden bazlar nelerdir?
A. mRNA'lar kodlayabilir
B. miRNA'lar kodlayabilir
C. levsel olmayan diziler olabilir
D. Transkripsiyon iin dzenleyici diziler olarak hizmet edebilir.
a Given that XX1X2Xn represents a random sample of .pdf
(a) Given that X=(X1,X2,,Xn) represents a random sample of size n from a Normal distribution with
unknown mean and unknown variance 2 denoted by N(,2) and the prior distribution of is N(0,02),
show that the posterior distribution of is N(1,12) where 1=n/2+1/02nX/2+0/02and12=n/2+1/021 (b)
Suppose the prior distribution of is N(12,4). A single observation x is taken. Find the posterior
distribution for in this case. In the case where 2=6 and x=10, find the posterior probability that >15..
A genetic disorder shows cytoplasmic inheritance A heteropl.pdf
A genetic disorder shows cytoplasmic inheritance. A heteroplasmic mother whose mitochondrial
genotype carries an allele for the genetic disorder could give rise to unaffected individuals if,
during fertilization a homoplasmic egg is used which had the normal allele that did not cause the
disorder.
a) True
b) False.
A generic queue is a general queue storage that can store an.pdf
A generic queue is a general queue storage that can store an unlimited number of objects.
In this lab, you need to implement two types of the generic queue including Queue and Deque.
The Java code of this module includes a class that represent Queues and was mentioned briefly
during the lecture, therefore if you need to, please revisit the java code.
The second special type of containers is called a Deque. A deque is a special type of queue that is
double ended. It means that elements can be added to the queue at both ends and removing can
be from both ends. In essence, a deque follows both a FIFO (First in, First out) and a LIFO (Last
In, First Out) rules to insert/remove from/to the queue. This means that a deque can play the role
of a queue and a stack at the same time. You can imagine a stack as a plate dispenser. When you
add a plate, you add it to the top of the dispenser. When you remove a plate, you remove it from
the top again. However, for a queue, you can imagine a queue of students waiting in line. The first
student who comes into the line is the first student served and hence removed from the line.
For the stack behavior of a deque, there are two main and two auxiliary methods:
push(object): This method adds the object to the stack. IN other words, the object is added at the
front.
pop(): removes the last element that was added, from the stack. In other words, the object is
removed from the front.
top(): returns the element, which is at the top of the stack, without removing it.
getSize(): returns the number of elements in the stack.
Your job for this lab, is to complete GenericQueue, Queue and Deque class. I have written a
comment, where you need to insert your code.
3.1. Class GenericQueue
The names of the methods in this class explain themselves. If you need more clarification, please
read the javaDoc for these methods.
3.2. Class Queue
While Queue Is-A generic queue, it follows the rule of Queue data structure (FIFO), therefore all
the methods should be implemented in a way that the rule is followed.
This class has an instance variable that holds all the items. The rest of the methods are self-
explanatory.
3.3. Class Deque
The Deque class is a special type of a Queue that was explained above. So the deque acts as a
queue and has additional methods that represent the stack behaviour.
This class has a constructor that uses the list that contains the data. To implement the rest of the
methods see their javaDoc.
import java.util.*;
import java.io.*;
/**
* This class is a GenericQueue that holds an unlimited number of
* objects. It is able to remove objects and add objects.
*/
public class GenericQueue {
// No instance variable should be defined for this class.
/**
* This method adds the <code> obj </code> to the GenericQueue.
* @param obj is the object that is added to the GenericQueue.
*/
void add(Object object) {
// insert your code here
}
/**
* This method removes the object from the GenericQueue
* @return returns the removed.
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He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
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Digital Tools and AI for Teaching Learning and Research
This Presentation in details discusses on Digital Tools and AI for Teaching Learning and Research
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Solid waste management & Types of Basic civil Engineering notes by DJ Sir.pptx
Solid waste management & Types of Basic civil Engineering notes by DJ Sir
Types of SWM
Liquid wastes
Gaseous wastes
Solid wastes.
CLASSIFICATION OF SOLID WASTE:
Based on their sources of origin
Based on physical nature
SYSTEMS FOR SOLID WASTE MANAGEMENT:
METHODS FOR DISPOSAL OF THE SOLID WASTE:
OPEN DUMPS:
LANDFILLS:
Sanitary landfills
COMPOSTING
Different stages of composting
VERMICOMPOSTING:
Vermicomposting process:
Encapsulation:
Incineration
MANAGEMENT OF SOLID WASTE:
Refuse
Reuse
Recycle
Reduce
FACTORS AFFECTING SOLID WASTE MANAGEMENT:
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Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
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