More Related Content Similar to Microbial interactions between humans and the built environment (Lake Arrowhead Microbial Genomics 2016) (20) Microbial interactions between humans and the built environment (Lake Arrowhead Microbial Genomics 2016)1. MICROBIAL INTERACTIONS
BETWEEN HUMANS & THE
BUILT ENVIRONMENT
ROXANA HICKEY
GREEN LAB & BioBE CENTER // UNIVERSITY OF OREGON
LAKE ARROWHEAD MICROBIAL GENOMICS // 09.21.16
@roxana_hickey
Credit: Gwenda Kacsor of Oregon Quarterly
2. © University of Oregon BioBE Center (2016) Credit: Tomasz Sienicki via Wikimedia Commons
Credit: Aptem via Wikimedia CommonsCredit: BrendelSignature via Wikimedia Commons
Credit: Malate269 via Wikimedia Commons
3. © University of Oregon BioBE Center (2016)
BIOLOGY & THE BUILT
ENVIRONMENT CENTER
4. © University of Oregon BioBE Center (2016)
BIOLOGY & THE BUILT ENVIRONMENT CENTER @ UNIVERSITY OF OREGON
http://biobe.uoregon.edu/
5. © University of Oregon BioBE Center (2016)
BIOLOGY & THE BUILT ENVIRONMENT CENTER @ UNIVERSITY OF OREGON
http://biobe.uoregon.edu/
ASHLEY BATEMAN
PHD CANDIDATE
CLARISSE
BETANCOURT ROMÁN
RESEARCH ASSISTANT
GZ CHARLIE BROWN
BIOBE CO-FOUNDER
& ESBL DIRECTOR
ASHKAAN
FAHIMIPOUR
POSTDOC
JESSICA GREEN
BIOBE CO-FOUNDER
& CO-DIRECTOR
ROXANA HICKEY
POSTDOC
JEFF KLINE
RESEARCH ASSOCIATE
GWYNNE
MHUIREACH
PHD CANDIDATE
DALE NORTHCUTT
SR RESEARCH
ASSISTANT
JASON STENSON
RESEARCH ASSISTANT
KEVIN VAN DEN
WYMELENBERG
ESBL DIRECTOR &
BIOBE CO-DIRECTOR
KYLA SIEMENS
RESEARCH ASSISTANT
HANNAH WILSON
RESEARCH ASSISTANT
6. © University of Oregon BioBE Center (2016)
BIOLOGY & THE BUILT ENVIRONMENT CENTER @ UNIVERSITY OF OREGON
http://biobe.uoregon.edu/
7. © University of Oregon BioBE Center (2016)
BIOLOGY & THE BUILT ENVIRONMENT CENTER @ UNIVERSITY OF OREGON
http://biobe.uoregon.edu/
8. © University of Oregon BioBE Center (2016)
HOW DOES THE DESIGN, OPERATION &
OCCUPANCY OF BUILDINGS INFLUENCE
THE INDOOR MICROBIOME?
9. © University of Oregon BioBE Center (2016)
A BRIEF HISTORY OF BioBE RESEARCH
Founded c/o Alfred P.
Sloan Foundation grant
2010
Ventilation source &
hospital room air
Kembel et al. ISME 2012
Architecture, ventilation strategy, occupancy
influence university building air & dust
Meadow et al. Indoor Air 2013
Kembel et al. PLOS One 2014
Microbiome of classroom surfaces
related to human contact
Meadow et al. Microbiome 2014
Human bioaerosols: the
microbial cloud
Meadow et al. PeerJ 2015
Urban green space &
outdoor air microbiome
Mhuireach et al. Env Sci & Tech 2016
Antimicrobials & antibiotic
resistance genes in dust
Hartmann et al. ES&T 2016
11. © University of Oregon BioBE Center (2016)
Kembel et al. ISME 2012
CAP 1
NMDS1
Deinococcus
Achromonobacter
Spiroplasma
Roseomonas
Rhabdochlamydia
Lysobacter
Lysinibaccillus
Pigmentiphaga
Acidisphaera
Brenneria
Methylobacterium
Sphingomonas
Streptococcus
Rhizobium
Mycoplana
Agrobacterium
Brevundimonas
mechanical window
p=0.005
constrained inertia=2.5%
a
0.05
0.10
0.15
0.20
0.25
0.30
Deinococcusrelativeabundance
air supply
mechanical window
p<0.001
b
0.02
0.04
0.06
0.08
0.10
0.12
0.14
Methylobacteriumrelativeabundance
air supply
mechanical window
p<0.001
c
North side:
Window supply
South side:
Mechanical supply
Kembel et al. PLOS One 2014; Meadow et al. Indoor Air 2013
Meadow et al. PeerJ 2015
12. © University of Oregon BioBE Center (2016)
Kembel et al. ISME 2012
CAP 1
NMDS1
Deinococcus
Achromonobacter
Spiroplasma
Roseomonas
Rhabdochlamydia
Lysobacter
Lysinibaccillus
Pigmentiphaga
Acidisphaera
Brenneria
Methylobacterium
Sphingomonas
Streptococcus
Rhizobium
Mycoplana
Agrobacterium
Brevundimonas
mechanical window
p=0.005
constrained inertia=2.5%
a
0.05
0.10
0.15
0.20
0.25
0.30
Deinococcusrelativeabundance
air supply
mechanical window
p<0.001
b
0.02
0.04
0.06
0.08
0.10
0.12
0.14
Methylobacteriumrelativeabundance
air supply
mechanical window
p<0.001
c
North side:
Window supply
South side:
Mechanical supply
Kembel et al. PLOS One 2014; Meadow et al. Indoor Air 2013
Meadow et al. PeerJ 2015
ARCHITECTURAL DESIGN, VENTILATION & OCCUPANCY
INFLUENCE THE MICROBIOME OF THE BUILT ENVIRONMENT
13. © University of Oregon BioBE Center (2016)
CURRENT RESEARCH @ BioBE
Effects of light source
(UV, visible) on dust
microbiome viability
Home weatherization &
microbiome diversity +
homeowner health, behavior
Multiple-occupancy &
ventilation effects on
microbial cloud
Microbial transmission from
plants & dogs to human skin
via the built environment
Child gut microbiome, home
dust & psychological stress
Antimicrobials &
antibiotic resistance
in athletic facilities
Theoretical models of
building occupancy &
microbiome diversity
14. © University of Oregon BioBE Center (2016)
HUMAN INTERACTIONS WITH THE BUILT ENVIRONMENT
ANTIMICROBIAL
CHEMICALS & THE
BUILT ENVIRONMENT
MICROBIOME
COLONIZATION OF THE
HUMAN SKIN
MICROBIOME VIA THE
BUILT ENVIRONMENT
CLOUDS IN A CROWD:
DISPERSAL OF HUMAN
BIOAEROSOLS
15. © University of Oregon BioBE Center (2016)
HUMAN INTERACTIONS WITH THE BUILT ENVIRONMENT
ANTIMICROBIAL
CHEMICALS & THE
BUILT ENVIRONMENT
MICROBIOME
COLONIZATION OF THE
HUMAN SKIN
MICROBIOME VIA THE
BUILT ENVIRONMENT
CLOUDS IN A CROWD:
DISPERSAL OF HUMAN
BIOAEROSOLS
16. © University of Oregon BioBE Center (2016)
ANTIMICROBIAL CHEMICALS
& RESISTANCE IN THE BUILT
ENVIRONMENT
ERICA HARTMANN
@ericamhartmann
17. © University of Oregon BioBE Center (2016)
ANTIMICROBIAL CHEMICALS IN THE BUILT ENVIRONMENT
STERILIZING THE BUILT ENVIRONMENT
18. © University of Oregon BioBE Center (2016) Source: Collegiate Images via Getty Images
GERLINGER HALL, UNIVERSITY OF OREGON
Est. 1921
HOW DOES THE CONCENTRATION OF ANTIMICROBIAL
CHEMICALS CORRELATE WITH THE MICROBIOME &
ANTIBIOTIC RESISTANCE GENES IN INDOOR DUST?
20. © University of Oregon BioBE Center (2016)
EXPERIMENTAL DESIGN
classrooms
gyms
offices
circulation
laundry
lockers
building support
pool
ANTIMICROBIALS
(LC-MS/MS)
triclosan
triclocarban
methylparaben
ethylparaben
propylparaben
butylparaben
ROLF HALDEN
ARIZONA STATE
CURTIS
HUTTENHOWER
HARVARD SPH
MICROBIOME &
RESISTOME
SHOTGUN
METAGENOMICS
ShortBRED &
HAllA w/ CARD
database
16S rRNA
UPARSE &
QIIME
44 VACUUM
DUST SAMPLES
21. © University of Oregon BioBE Center (2016)
LOWER OVERALL LEVELS OF ANTIBIOTIC RESISTANCE GENE ABUNDANCE
Hartmann, Hickey, Hsu et al. Env Sci Tech 2016
22. © University of Oregon BioBE Center (2016)
SIX RESISTANCE GENES SIGNIFICANTLY ASSOCIATED WITH [CHEMICALS]
Gene Description
Similarity
Score
q
erm(X) 23S rRNA transferase 0.86 0.036
tet(K) tetracycline resistance efflux pump 0.83 0.036
vga(A) streptogramin res. efflux pump 0.65 0.036
erm(33) 23S rRNA transferase 0.73 0.036
erm(C) 23S rRNA transferase 0.68 0.036
cmr chloramphenicol res. efflux pump 0.58 0.048
Hartmann, Hickey, Hsu et al. Env Sci Tech 2016
TRICLOSANMETHYLPARABEN
23. © University of Oregon BioBE Center (2016)
NO MORE TRICLOSAN FOR YOU(R PERSONAL CARE PRODUCTS)
http://www.fda.gov/ForConsumers/ConsumerUpdates/ucm378393.htm
24. © University of Oregon BioBE Center (2016)
HUMAN INTERACTIONS WITH THE BUILT ENVIRONMENT
ANTIMICROBIAL
CHEMICALS & THE
BUILT ENVIRONMENT
MICROBIOME
COLONIZATION OF THE
HUMAN SKIN
MICROBIOME VIA THE
BUILT ENVIRONMENT
CLOUDS IN A CROWD:
DISPERSAL OF HUMAN
BIOAEROSOLS
25. © University of Oregon BioBE Center (2016)
CLOUDS IN A CROWD:
DISPERSAL OF HUMAN
BIOAEROSOLS
JAMES MEADOW
@JamesMeadow
ASHKAAN
FAHIMIPOUR
@AshkaanF
26. © University of Oregon BioBE Center (2016)
CLIMATE CHAMBER
UO Energy Studies in Buildings
Laboratory, Portland, OR
Control surface & air temperature,
humidity, air velocity
27. © University of Oregon BioBE Center (2016)
SAMPLING THE HUMAN MICROBIAL CLOUD
Occupied
Unoccupied
Air Filter (12 per treatment)
1 m
0.15 m
Settling Dish (6 per treatment)
Particle Counter (1 per treatment)
2.4 m
3.7m
2.4 m
2.9m
Meadow et al. PeerJ 2015
28. © University of Oregon BioBE Center (2016)
AN OCCUPIED ROOM IS DISTINCT FROM AN UNOCCUPIED ROOM
Meadow et al. PeerJ 2015
29. © University of Oregon BioBE Center (2016)
SPACES OCCUPIED BY DIFFERENT PEOPLE HAVE UNIQUE MICROBIAL PATTERNS
Meadow et al. PeerJ 2015
30. © University of Oregon BioBE Center (2016)
http://www.sciencefriday.com/videos/
your-very-special-microbial-cloud/
31. © University of Oregon BioBE Center (2016)
CAN INDIVIDUALS BE DISTINGUISHED BY THEIR
MICROBIAL CLOUD IN A ROOM OF PEOPLE?
32. © University of Oregon BioBE Center (2016)
EXPERIMENTAL DESIGN: SOLO RUN
4’ 2’2’
4’2’4’2’
10’1’
20”
1’
10”
Filter Cup
T, RH Sensor
MRT Sensor
4’ 2’2’
9’-6”1’
30”
1’
40”
Diagram by Jason Stenson, Energy Studies in Buildings Laboratory
33. © University of Oregon BioBE Center (2016)
EXPERIMENTAL DESIGN: SOLO RUN
4’ 2’2’
4’2’4’2’
10’1’
20”
1’
10”
Filter Cup
T, RH Sensor
MRT Sensor
4’ 2’2’
9’-6”1’
30”
1’
40”
Diagram by Jason Stenson, Energy Studies in Buildings Laboratory
CAN WE DETECT SPECIFIC MEMBERS OF THE SKIN
MICROBIOME IN BIOAEROSOLS & MEASURE THEIR
DISPERSAL ACROSS THE ROOM?
34. © University of Oregon BioBE Center (2016)
EXPERIMENTAL DESIGN: GROUP RUN
4’ 2’2’
4’2’4’2’
10’1’
20”
1’
10”
Filter Cup
T, RH Sensor
MRT Sensor
4’ 2’2’
9’-6”1’
30”
1’
40”
Diagram by Jason Stenson, Energy Studies in Buildings Laboratory
35. © University of Oregon BioBE Center (2016)
EXPERIMENTAL DESIGN: GROUP RUN
4’ 2’2’
4’2’4’2’
10’1’
20”
1’
10”
Filter Cup
T, RH Sensor
MRT Sensor
4’ 2’2’
9’-6”1’
30”
1’
40”
Diagram by Jason Stenson, Energy Studies in Buildings Laboratory
CAN WE PREDICT WHO WAS PRESENT IN THE ROOM
BASED ON THEIR SKIN MICROBIOME & THE
COMPOSITION OF BIOAEROSOLS?
36. © University of Oregon BioBE Center (2016)
HUMAN INTERACTIONS WITH THE BUILT ENVIRONMENT
ANTIMICROBIAL
CHEMICALS & THE
BUILT ENVIRONMENT
MICROBIOME
COLONIZATION OF THE
HUMAN SKIN
MICROBIOME VIA THE
BUILT ENVIRONMENT
CLOUDS IN A CROWD:
DISPERSAL OF HUMAN
BIOAEROSOLS
37. © University of Oregon BioBE Center (2016)
MICROBIAL COLONIZATION
OF SKIN VIA THE BUILT
ENVIRONMENT
ASHLEY BATEMAN
@MicrobesRock
38. © University of Oregon BioBE Center (2016)
SOURCES OF MICROBES IN THE BUILT ENVIRONMENT
Prussin & Marr Microbiome 2015
HUMANS PETS
PLUMBING
SYSTEMS
PLANTS
VENTILATION
SYSTEMS MOLD
RESUSPENSION
OF SETTLED DUST
OUTDOOR
AIR
39. © University of Oregon BioBE Center (2016)
DOES MICROBIAL EXCHANGE OCCUR TO AND FROM THE BUILT ENVIRONMENT?
40. © University of Oregon BioBE Center (2016)
ROUTES OF MICROBIAL TRANSMISSION TO THE HUMAN SKIN MICROBIOME
SURFACE
DIRECT CONTACT BE SURFACE INTERMEDIATE BIOAEROSOL INTERMEDIATE
42. © University of Oregon BioBE Center (2016)
HOW ARE MICROBES EXCHANGED AMONG
BUILDINGS, AIR & PEOPLE?
ARE THERE ‘GENERAL LAWS’ IN BUILDING ECOLOGY?
WHAT IS THE RELATIONSHIP BETWEEN THE BUILT
ENVIRONMENT, HUMAN MICROBIOME & HEALTH?
Source: BioBE Center with Cameron Slayden of Cosmocyte
43. © University of Oregon BioBE Center (2016)
OUR TEAM
Jessica Green
Kevin Van Den Wymelenberg
G.Z. Charlie Brown
Curtis Huttenhower (Harvard)
Rolf Halden (Arizona State)
Alejandro Manzo
Gwynne Mhuireach
Max Moriyama
Dale Northcutt
Tim O’Connor
Iman Rejaie
Maria Sarao
Andy Siemens
Kyla Siemens
Jason Stenson
Paul Ward
Hannah Wilson
Adam Altrichter
Dan Aughenbaugh
Ashley Bateman
Clarisse Betancourt
Brendan Bohannan
Ashkaan Fahimipour
Mark Fretz
Erica Hartmann
Roxana Hickey
Keith Herkert
Evan Jones
Ann Klein
Jeff Kline
@roxana_hickey
@JessicaLeeGreen
@Wymelenberg
@ericamhartmann
@AshkaanF
@MicrobesRock
@betancourt_cm