Campbell6e lecture ch5

Chapter Five Protein Purification and Characterization Techniques

Isolation of Proteins from Cells ,[object Object],[object Object],[object Object]

How We Get Proteins Out of Cells

Salting Out ,[object Object],[object Object],[object Object],[object Object],[object Object]

Differential Centrifugation ,[object Object],[object Object]

Column Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Size-Exclusion/Gel-Filtration ,[object Object],[object Object],[object Object],[object Object]

Size Exclusion/Gel-filtration (Cont’d)

Affinity Chromatography ,[object Object],[object Object]

Ion Exchange ,[object Object],[object Object],[object Object]

Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Electrophoresis (Cont’d) ,[object Object],[object Object],[object Object]

Isoelectric Focusing ,[object Object],[object Object],[object Object],[object Object]

Primary Structure Determination ,[object Object],[object Object],[object Object],[object Object],[object Object]

Primary Structure Determination

Protein Cleavage ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Cleavage by CnBr ,[object Object]

Determining Protein Sequence ,[object Object],[object Object],[object Object]

Peptide Sequencing ,[object Object],[object Object],[object Object]

Campbell6e lecture ch5

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