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INTERNATIONAL JOURNAL OF CIVIL ENGINEERING 
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
AND TECHNOLOGY (IJCIET) 
ISSN 0976 – 6308 (Print) 
ISSN 0976 – 6316(Online) 
Volume 5, Issue 9, September (2014), pp. 103-113 
© IAEME: www.iaeme.com/Ijciet.asp 
Journal Impact Factor (2014): 7.9290 (Calculated by GISI) 
www.jifactor.com 
103 
 
IJCIET 
©IAEME 
STUDY OF LABORATORY WATER CONSUMPTIVE USE AND 
RECYCLABILITY 
F. A. Oginni1* and O. E. Alaka1** 
1Department of Civil Engineering, Osun State University, Oshogbo, Nigeria 
*kafnog@gmail.com; oginnifa@uniosun.edu.ng 
**Oluwaseunalaka@gmail.com 
ABSTRACT 
The present challenges of inadequacy of water supply in tertiary institutions may degenerate 
to rationing which is counterproductive for research and development. This study attempts to solve 
this issue by determining laboratory water consumptive use and recyclability in Osun State 
University, Nigeria. The number of water taps/valves per laboratory was evaluated. A pilot study for 
determining laboratory water use/tap was carried out and estimated to 64.26Liters/tap. Water use for 
each laboratory practical session was then determined and categorized as A, B and C for 1,000 – 
2,000lit/batch; 500 – 1,000lit/batch and 0 – 500lit/batch respectively. 4 labs were then selected for 
detailed study based on the categorization and practical courses. These are Industrial Chemistry, 
(ICH); Biochemistry, (BCH); Medical, (MED); and Water and Environmental, (WE) labs. Physical 
and chemical analyses for the lab influent and effluent were undertaken. The effluent was also 
analyzed for toxic chemicals to serve as recyclability indicators. These are Ammonia, Phosphate, 
Chromium (Hexa/6+), Copper, Aluminum, Silica, Sulphate, Molybdate, Bromine total. Differences 
between influent and effluent parameters were considered as percentages of influent values and used 
as treatment levels required for recyclability/lab. Results indicated that effluent from Biochemistry 
lab requires the greatest treatment levels for total hardness, magnesium hardness, total alkalinity 
fluoride, total chlorine, free chlorine, and zinc. Zinc deposits during the Biochemistry and Industrial 
Chemistry practical were 1900% and 539% compared with the influent values, while deposits for the 
other two labs were below 20%. Chromium and Sulphate levels were found to be greater than the 
SON permissible levels within the Biochemistry and Industrial Chemistry labs. It is recommended 
that: (i) treatment facility should give further attention to these parameters of concern; and (ii) the 
influent water be analyzed to determine levels of the other chemicals, considered for detection of 
toxic materials and as indicators of recyclability, so as to monitor any development at effluent level. 
Caution should be exercised in allowing concentrations of some parameters whose safe limits were 
not specified by SON to build up.
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
Keywords: Laboratory, Influent, Effluent, Wastewater, Recyclability, Chemicals, Parameters. 
104 
1. INTRODUCTION 
 
Inadequacy of water is a too common in many Communities, Organizations, and Institutions 
in Nigeria. Even many settlements that claim to be able to supply their systems with water 24/7 often 
get to the stage where water conservation measures have to come to their rescues. Osun State 
University located is located around a coordinate of 7046’N and 4034’ E [3], [11] . The need for 
water in our Institutions of learning can never be overemphasized. Efforts are always being geared 
towards meeting water needs in our society that one may not be wrong if it is said that one of our 
purposes in life is working to supply our daily water needs to include the variation of water quality 
within the sources [10], [5], [9]. Treated wastewater can be reused as drinking water, in industry 
(cooling towers), in artificial recharge of aquifers, in agriculture (70% of Israel's irrigated agriculture 
is based on highly purified wastewater) and in the rehabilitation of natural ecosystems [13]. 
Professionals and Trainers of on-coming Young Engineering Researchers one should also be 
interested in providing adequate and efficient water in our laboratories. The use of reclaimed 
wastewater for irrigation can be a lesson for other sectors of water needs, but only a few people have 
expertise in the full range of technology involved [12], [8]. In order to effectively rise up to this 
challenge, we are proposing a programme that will embark on the reuse of our waste water from the 
laboratories. This will solve the problem of inadequate provision of water in tertiary institutions. 
2.0 MATERIALS AND METHODS 
2.1 Project Area 
The Project area is within Osun State University, Main Campus. It is located in Oke-Baale 
area in Oshogbo. Oshogbo is the capital of Osun State, Nigeria. The laboratories on the campus are 
located in the Colleges of Science, Engineering and Technology; Health Sciences and the University 
Health Center. 
There are 24(No) Laboratories on the campus distributed as follows: 
9 (no) in the College of SET Building 
9(no) in the College of SET Extension Bldg 
2 (no) in the Engineering Workshop 
2(no) in the College of Health Sciences 
2(no) in the University Health Centre (Medical Clinic) 
2.2 Determination of Water Use per Laboratory Session 
Laboratories (labs) can only be found where there are academic and medical services 
activities. Non-academic activities majorly take place in the Administrative Building. The campus 
accommodates basic lab –based units. These are College of Science, Engineering and Technology 
(College of SET); Engineering Workshop; College of Health Sciences and University Health 
Center/Medical Clinic.
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
105 
 
The total number of laboratories for each unit was determined. Estimation of laboratory water 
use per tap was carried out following the procedure indicated below: 
1. Estimation of the distribution of laboratories on the campus 
2. Assessment of existing labs to determine lab sizes and total number per lab-based units 
3. Estimation of number of water taps/(spigots) for each laboratory practical use 
4. Determine the lab practical water use per tap 
2.2.1 Pilot study for determination of lab practical water use per tap 
If Vi liters is the volume of water collected from ith tap running for a time ti sec, the average 
discharge, Q for n = 4 taps is computed as water use per tap using equation (1) below: 
……… (1) 
n = 4 
Q =  Vi 
ti 
i = 1 
1 
n 
For the pilot study four taps/spigots were randomly selected and turned on simultaneously to 
run at full opening of their circumference. Discharge for a 2sec. time run from each spigot was taken 
with the aid of calibrated beakers and stop watch. Volume obtained for each run were measured as 
70, 76, 69, and 65liters. These gave discharges, of 35, 38, 34.5 and 32.5liters/sec, computed to an 
average discharge of 35liters/sec. 
2.2.2 Pilot study for determination of water use per lab session 
To determine water use per lab session, the time for water use per lab session was determined 
by carrying out a pilot study to determine the effective time for practical or lab session. Students are 
usually grouped into as many as 4 batches for practical purposes depending on the available space 
for the practical session. Time spent on practical use of the laboratory varies from 2 to 6 hours 
depending on how many groups to use the lab for the day. Time for a grouped/batch practical is 
usually a maximum of 2hours. 
With a random selection, an average total time of practical or water use time for Industrial 
Chemistry laboratory as an example was determined through the aid of a stop watch as follows: 
Tap/valve discharge before practical’s for rinsing of practical instruments = 9.8 minutes 
Tap/valve discharge during practical’s for experimental procedure = 5.3 minutes 
Tap/valve discharge after practical’s for final rinsing and washing of hands = 15.5 minutes 
Total time of practical = 30.6 minutes 
Average discharge = 35ml/sec 
Volume of water use/tap = 30.6min.x35ml/sec 
= (30.6 x 60 x 35)mL /1000mL/L} 
= 64.26 Liters/tap 
2.3 Selection of Laboratories for Influent and Effluent Analyses 
The no of taps for each lab are indicated in Table 1. Water consumption/lab is determined as 
products of each lab and volume of water use per tap. Values for each laboratory are shown in Table 
1. The water consumption/lab is then classified into A, B or C, according to ranges as 1,000- 
2,000L/lab; 500-1000L/lab; and 0-500L/lab respectively.
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
106 
 
Table 1: Analyses of Water Use per Lab-Session 
S/N Laboratory Lab Size 
(m2) 
Taps 
(No) 
Water use 
(Lit/Lab) 
Class 
1 Industrial Chem. 375 25 1606.5 A 
2 Bio-Chemistry 150 15 963.9 B 
3 Maths Lab. 100 0 C 
4 Micro-Biology 150 15 963.9 B 
5 Physics Advanced Lab 375 0 0 C 
6 Digital Electronics 150 0 0 C 
7 Hard Ware  Net. Lab 375 0 0 C 
8 Research Lab. 100 10 642.6 B 
9 Water And Environmental Lab, 100 1 64.26 C 
10 Physics Lab 1 150 0 0 C 
11 Physics Electronics Workshop Lab 2 100 0 0 C 
12 Physics Lab 3 375 0 0 C 
13 Biology Lab 375 25 1606.5 A 
14 Chemistry Lab 375 25 1606.5 A 
15 Geology Lab 1 150 2 128.52 C 
16 Geology Lab 2 375 2 128.52 C 
17 Cisco Lab. 375 0 0 C 
18 Applied Electricity  Electronics Lab 150 0 0 C 
19 Materials And Structures Lab. 150 2 128.52 C 
20 Metal Workshop 150 2 128.52 C 
21 Bio-Chemistry Lab. 150 15 963.9 B 
22 Physiology Lab. 150 15 963.9 B 
23 Doctor’s Office (Medical lab) 25 1 64.26 C 
24 Injection Room (Medical lab) 25 1 64.26 C 
The distribution of the categorized water use is shown in Figure 1. Four laboratories were 
selected based on the class and respective laboratory courses on the campus. The selected
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
laboratories are Industrial chemistry lab, (ICH), Biochemistry lab, (BCH), Medical lab (MED) and 
Water and environmental lab (WE).
107 
 
 
 
Figure 1: Water Consumption/Lab-Session 
2.5 Materials and Experimental Protocols 
The materials required to carry out the above exercise include; 
Photometer Test tubes Plastic container or kegs with air tight cover 
Measuring cylinder Distilled water 70% ethanol 
Cotton wool pH meter Turbidity Meter 
Reagents Stop watch hose 
2.5.1 Sterilization of Materials 
All glass wares were thoroughly washed with detergent solution, rinsed with several changes 
of distilled water and subsequently allowed to drain, after which they were sterilized or disinfected 
with 70% ethanol. The working bench was also swabbed with 70% alcohol to have a sterile 
environment. 
2.5.2 Labelling of samples 
Samples collected were labelled according to the tabulated codes shown in Table 2 below to 
avoid mix up of different samples. 
Table 2: Labeling of Samples 
LABORATORY INFLUENT LABELLING EFLUENT LABELLING 
Sample 1 Sample 2 Sample 1 Sample 2 
Industrial chemistry lab ICH-IS1 ICH-IS2 ICH-ES1 ICH-ES2 
Bio-chemistry lab BCH-IS1 BCH- IS2 BCH-ES1 BCH-ES2 
Micro-biology lab MCB-IS1 MCB-IS2 MCB-ES1 MCB-ES2 
Health centre HC-IS1 HC-IS2 HC-ES1 HC-ES2
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
108 
2.5.3 Collections of Samples 
 
Utmost and scrupulous care was taken in the collection of the water and waste water samples 
to ensure the samples are representative of the water and waste water desired to the examined and to 
avoid accidental contamination of the samples. 
To collect influent samples, the outlet of the running tap of the laboratories were disinfected 
using 70% ethanol with the aid of a swab and was allowed to run for at least five minutes before 
taking the sample into an air tight container or kegs in order to stabilize its temperature and to carry 
out analysis on the influent samples of each laboratory. The tap water samples were transported to 
the water and environmental laboratory for analysis in ice packs. 
For the collection of effluent samples, the sewage drain of the laboratories was located and 
samples were taken with tapping the sewage channel or drain of all sinks and wash hand basins in all 
the laboratories. This was channeled into an air tight container or big keg of about 50litres with a 
hose in order to stabilize its temperature and was refrigerated to carry out analysis on the second day. 
The container or kegs should not be filled above 90% to avoid spillage during transportation. The 
samples were transported in ice packs to the water and environmental laboratories for analysis. 
2.6 Physico-chemical Analysis of Influent and Effluent 
Physical observations were made of the colour and odour of the samples while other physical 
properties, pH, temperature and turbidity were measured with pH meter, thermometer and 
turbidimeter respectively. In determining the turbidity of the sample, a two part calibrated turbidity 
tube was used with calibrations from 5-25 turbidity units. The joined tubes were held over a white 
paper, while slowly pouring the water sample into the tube until the black cross at the bottom was no 
longer visible. At this point the reading was taken from the side of the tube as the turbidity value of 
the water sample. 
Alkalinity, Total Hardness and the presence of Chlorine (DPD), Phosphate, Zinc, Nitrate, 
Nitrite, Calcium were determined in the influent. The Automatic Wavelength Photometer was used 
in carrying out this exercise. Standard procedures were followed with special attentions given to 
various notes for each of the above parameters as specified in the relevant manual. Analyses of the 
samples were carried out at the Water and Environmental laboratory in Osun State University and 
RUWESA in Osun State Government Secretariat in Abere, Oshogbo. 
2.7 Estimation of Recyclability 
The effluent is further analyzed for toxic/hazardous chemicals to serve as recyclability 
indicators. The additional tests were for Ammonia, Chromium (Hexa/6+), Copper, Aluminum, 
Silica, Sulphate, Molybdate and Bromine total. These tests are for advanced analysis of waste water 
sample from laboratories and industries which help in the detection of toxic materials and 
recyclability of waste water sample. The effluent hazardous chemical levels determined. Differences 
in the influent and effluent were estimated as percentages of influent. These are to serve as treatment 
levels required for recyclability. 
3.0 RESULTS ANALYSIS AND DISCUSSION 
Results of the various physical and chemical waste water quality parameters for both influent 
and effluent are obtained for further analysis. Analysis of the differences in the wastewater quality 
parameters between the influent and effluent materials from each laboratory are determined so as to 
indicate what has been lost through the activities in the laboratories. The differences are presented in 
percentage forms in Table 3, so as to evaluate degrees of degradations experienced in the use of the 
influent water into each laboratory. Items 1 to 8 deal with the physical qualities of both the influent
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 
and effluent waste water. The chemical properties are shown in items 9 to 23. The results obtained 
for additional toxic materials are indicated in Table 3. 
109 
3.1 Physiochemical evaluations 
 
The influent and effluent Physicochemical Parameters are shown in Table 3 which indicates 
the percentage differences in each laboratory. Results of the Toxic and Recyclability Indicator of 
Laboratory Effluent 
Table 3: % Difference Between Influent And Effluent Pysico-Chemical Parameters 
S/N Parameter Unit (%) Difference / Laboratories 
Bio Chem Ind Chem Medical Water  Env 
1 Colour TCU 15 15 15 15 
2 Odour - - - - 
3 Taste - - - - 
4 pH 37 23 -42 9 
5 Temperature OC 12 25 3 6 
6 Conductivity μS/cm - - - -119 
7 Turbidity NTU - - - - 
8 T.D.S Mg/L - - -221 -141 
9 O.R.P Mv -53 -129 -66 -59 
10 Chloride Mg/L -18 33 50 47 
11 Total Hardness Mg/L -602  -422 -140 
12 Calcium Hardness Mg/L 17 90 -650 -471 
13 Magnessium Hardness Mg/L -723 - -362 -86 
14 Total Chlorine Mg/L -189 96 79 29 
15 Free Chlorine Mg/L -280 100 -250 60 
16 Total Alkalinity Mg/L -574 -448 -88 -79 
17 Nitrite(NO2) Mg/L 31 73 8 2 
18 Nitrate(NO3) Mg/L Inconclusive 43 38 1 
19 Arsenic Mg/L - - - - 
20 Iron Mg/L 100 100 96 -12 
21 Manganese Mg/L 96 84 8 28 
22 Fluoride Mg/L -496 69 60 13 
23 Zinc Mg/L -1900 -539 -15 13
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 
The % differences in the Physical 
103-113 © IAEME 
Parameters could not be ascertained for colour 
effluent. Similarly, percentage differences for odor and taste 
than 15 (15) for both the influent and effluent 
influent and effluent only referred to as unobjectionable 
unobjectionable, (UO), while Turbidity % differen 
greater than 5, and therefore these differences cannot be determined. Conductivity 
values of some laboratories are greater than the photometer range. There is no arsenic from the 
analysis carried out hence the percent differences 
The percent differences in the chemical para 
levels of treatments required for recyclability. Activities in the Biochemistry lab presented the worst 
situation in the areas of total hard 
requirements as their percent differences are more of less between 500% and 720%. From the 
presentations of the total chlorine, free chlorine, this same laboratory needs more attention in 
disinfection of the waste water [6], [7] 
  
Figure 2: %Difference Between Influent and Effluent 
	 
 
 
 
 
 
 
 
 
The percent difference between influent water and effluent waste water presents the worst 
situation for Zinc in Table 3, getting to as great as 1900% and 539% for Biochemistry lab and 
Industrial Chemistry lab respectively. The other laboratories 
for the nitrate level for Biochemistry lab was inconclusive. 
Zinc levels, although there is no health impact currently associated with it, it should not be allowed 
to keep rising in a situation of recyclability. 
Results of tests of the parameters considered to be toxic undertaken only for the effluen 
waste water is shown in Table 4 
Organization of Nigeria, [14] and [15 
water [14], [2] and illustrated in Figure 
need to be watched, especially in the Biochemistry and Industrial Chemistry laboratories as they are 
currently greater than the permissible levels 
Although [14] did not specify safe limits for some parameters, caution should be exercised in 
allowing their concentrations to keep building up. There is therefore need for evaluation of these 
parameters indicted at the influent level so as to monitor any development. 
110 
and 
were not determined. 
parameters are presented in Figure 2 
hardness, magnesium hardness, total alkalinity and fluoride 
7]. 
, had differences lower than 20%. Results 
There is therefore the need 
aste 4. These are compared with the guidelines of the Standard 
4] 15], for quality assessment of good industrial water/ safe drinking 
3. It can be observed that Chromium and Sulphate levels that 
[1], [4]. They need to be treated before recycling. 
d – 6308 (Print), 
 
as it is less 
difference is 
total hardness 
meters to indicate the 
ness, to monitor the 
effluent 
. .
International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 
ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 
Table 4: Results of the Toxic 
S/N 
Waste Water 
Recyclability 
Indicator 
Unit 
1 Ammonia Mg/L 
2 Phosphate Mg/L 
3 Chromium Mg/L 
4 Alluminuim Mg/L 
5 Silica Mg/L 
6 Sulphate Mg/L 
7 Molybdate Mg/L 
8 Total Bromine Mg/L 
9 Hexavalent Chromium Mg/L 
10 Total Copper Mg/L 
11 Free Cooper Mg/L 
12 Sulphide Mg/L 
13 Chlorinedioxide Mg/L 
and Recyclability Indicator of Laboratory Effluent 
Laboratories SON 
BCH ICH MED WE SON Max 
Figure 3: Toxic and Recyclability Indicator of Lab Effluent
!##$ 
103-113 © IAEME 
111 
permitted 
0.32 0.04 0.46 0.15 1.00 
1.18 0.79 0.08 0.02 6.00 
0.14 0.10 0.03 0.01 0.05 
0.11 0.10 0.06 0.04 0.20 
    4.00 
175 170 56 80 100 
    
0.56 0.08 0.04 0.02 
0.13 0.28 0.23 0.01 
0.70 0.00 0.01 0.00 
0.58 0.00 0.00 0.00 
0.12 0.08 0.03 0.11 
0.16 0.04 0.01 0.02 
– 6308 (Print), 
 
Remark 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable 
Recyclable

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Laboratory water recycling study

  • 1. INTERNATIONAL JOURNAL OF CIVIL ENGINEERING International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME AND TECHNOLOGY (IJCIET) ISSN 0976 – 6308 (Print) ISSN 0976 – 6316(Online) Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME: www.iaeme.com/Ijciet.asp Journal Impact Factor (2014): 7.9290 (Calculated by GISI) www.jifactor.com 103 IJCIET ©IAEME STUDY OF LABORATORY WATER CONSUMPTIVE USE AND RECYCLABILITY F. A. Oginni1* and O. E. Alaka1** 1Department of Civil Engineering, Osun State University, Oshogbo, Nigeria *kafnog@gmail.com; oginnifa@uniosun.edu.ng **Oluwaseunalaka@gmail.com ABSTRACT The present challenges of inadequacy of water supply in tertiary institutions may degenerate to rationing which is counterproductive for research and development. This study attempts to solve this issue by determining laboratory water consumptive use and recyclability in Osun State University, Nigeria. The number of water taps/valves per laboratory was evaluated. A pilot study for determining laboratory water use/tap was carried out and estimated to 64.26Liters/tap. Water use for each laboratory practical session was then determined and categorized as A, B and C for 1,000 – 2,000lit/batch; 500 – 1,000lit/batch and 0 – 500lit/batch respectively. 4 labs were then selected for detailed study based on the categorization and practical courses. These are Industrial Chemistry, (ICH); Biochemistry, (BCH); Medical, (MED); and Water and Environmental, (WE) labs. Physical and chemical analyses for the lab influent and effluent were undertaken. The effluent was also analyzed for toxic chemicals to serve as recyclability indicators. These are Ammonia, Phosphate, Chromium (Hexa/6+), Copper, Aluminum, Silica, Sulphate, Molybdate, Bromine total. Differences between influent and effluent parameters were considered as percentages of influent values and used as treatment levels required for recyclability/lab. Results indicated that effluent from Biochemistry lab requires the greatest treatment levels for total hardness, magnesium hardness, total alkalinity fluoride, total chlorine, free chlorine, and zinc. Zinc deposits during the Biochemistry and Industrial Chemistry practical were 1900% and 539% compared with the influent values, while deposits for the other two labs were below 20%. Chromium and Sulphate levels were found to be greater than the SON permissible levels within the Biochemistry and Industrial Chemistry labs. It is recommended that: (i) treatment facility should give further attention to these parameters of concern; and (ii) the influent water be analyzed to determine levels of the other chemicals, considered for detection of toxic materials and as indicators of recyclability, so as to monitor any development at effluent level. Caution should be exercised in allowing concentrations of some parameters whose safe limits were not specified by SON to build up.
  • 2. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME Keywords: Laboratory, Influent, Effluent, Wastewater, Recyclability, Chemicals, Parameters. 104 1. INTRODUCTION Inadequacy of water is a too common in many Communities, Organizations, and Institutions in Nigeria. Even many settlements that claim to be able to supply their systems with water 24/7 often get to the stage where water conservation measures have to come to their rescues. Osun State University located is located around a coordinate of 7046’N and 4034’ E [3], [11] . The need for water in our Institutions of learning can never be overemphasized. Efforts are always being geared towards meeting water needs in our society that one may not be wrong if it is said that one of our purposes in life is working to supply our daily water needs to include the variation of water quality within the sources [10], [5], [9]. Treated wastewater can be reused as drinking water, in industry (cooling towers), in artificial recharge of aquifers, in agriculture (70% of Israel's irrigated agriculture is based on highly purified wastewater) and in the rehabilitation of natural ecosystems [13]. Professionals and Trainers of on-coming Young Engineering Researchers one should also be interested in providing adequate and efficient water in our laboratories. The use of reclaimed wastewater for irrigation can be a lesson for other sectors of water needs, but only a few people have expertise in the full range of technology involved [12], [8]. In order to effectively rise up to this challenge, we are proposing a programme that will embark on the reuse of our waste water from the laboratories. This will solve the problem of inadequate provision of water in tertiary institutions. 2.0 MATERIALS AND METHODS 2.1 Project Area The Project area is within Osun State University, Main Campus. It is located in Oke-Baale area in Oshogbo. Oshogbo is the capital of Osun State, Nigeria. The laboratories on the campus are located in the Colleges of Science, Engineering and Technology; Health Sciences and the University Health Center. There are 24(No) Laboratories on the campus distributed as follows: 9 (no) in the College of SET Building 9(no) in the College of SET Extension Bldg 2 (no) in the Engineering Workshop 2(no) in the College of Health Sciences 2(no) in the University Health Centre (Medical Clinic) 2.2 Determination of Water Use per Laboratory Session Laboratories (labs) can only be found where there are academic and medical services activities. Non-academic activities majorly take place in the Administrative Building. The campus accommodates basic lab –based units. These are College of Science, Engineering and Technology (College of SET); Engineering Workshop; College of Health Sciences and University Health Center/Medical Clinic.
  • 3. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 105 The total number of laboratories for each unit was determined. Estimation of laboratory water use per tap was carried out following the procedure indicated below: 1. Estimation of the distribution of laboratories on the campus 2. Assessment of existing labs to determine lab sizes and total number per lab-based units 3. Estimation of number of water taps/(spigots) for each laboratory practical use 4. Determine the lab practical water use per tap 2.2.1 Pilot study for determination of lab practical water use per tap If Vi liters is the volume of water collected from ith tap running for a time ti sec, the average discharge, Q for n = 4 taps is computed as water use per tap using equation (1) below: ……… (1) n = 4 Q = Vi ti i = 1 1 n For the pilot study four taps/spigots were randomly selected and turned on simultaneously to run at full opening of their circumference. Discharge for a 2sec. time run from each spigot was taken with the aid of calibrated beakers and stop watch. Volume obtained for each run were measured as 70, 76, 69, and 65liters. These gave discharges, of 35, 38, 34.5 and 32.5liters/sec, computed to an average discharge of 35liters/sec. 2.2.2 Pilot study for determination of water use per lab session To determine water use per lab session, the time for water use per lab session was determined by carrying out a pilot study to determine the effective time for practical or lab session. Students are usually grouped into as many as 4 batches for practical purposes depending on the available space for the practical session. Time spent on practical use of the laboratory varies from 2 to 6 hours depending on how many groups to use the lab for the day. Time for a grouped/batch practical is usually a maximum of 2hours. With a random selection, an average total time of practical or water use time for Industrial Chemistry laboratory as an example was determined through the aid of a stop watch as follows: Tap/valve discharge before practical’s for rinsing of practical instruments = 9.8 minutes Tap/valve discharge during practical’s for experimental procedure = 5.3 minutes Tap/valve discharge after practical’s for final rinsing and washing of hands = 15.5 minutes Total time of practical = 30.6 minutes Average discharge = 35ml/sec Volume of water use/tap = 30.6min.x35ml/sec = (30.6 x 60 x 35)mL /1000mL/L} = 64.26 Liters/tap 2.3 Selection of Laboratories for Influent and Effluent Analyses The no of taps for each lab are indicated in Table 1. Water consumption/lab is determined as products of each lab and volume of water use per tap. Values for each laboratory are shown in Table 1. The water consumption/lab is then classified into A, B or C, according to ranges as 1,000- 2,000L/lab; 500-1000L/lab; and 0-500L/lab respectively.
  • 4. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 106 Table 1: Analyses of Water Use per Lab-Session S/N Laboratory Lab Size (m2) Taps (No) Water use (Lit/Lab) Class 1 Industrial Chem. 375 25 1606.5 A 2 Bio-Chemistry 150 15 963.9 B 3 Maths Lab. 100 0 C 4 Micro-Biology 150 15 963.9 B 5 Physics Advanced Lab 375 0 0 C 6 Digital Electronics 150 0 0 C 7 Hard Ware Net. Lab 375 0 0 C 8 Research Lab. 100 10 642.6 B 9 Water And Environmental Lab, 100 1 64.26 C 10 Physics Lab 1 150 0 0 C 11 Physics Electronics Workshop Lab 2 100 0 0 C 12 Physics Lab 3 375 0 0 C 13 Biology Lab 375 25 1606.5 A 14 Chemistry Lab 375 25 1606.5 A 15 Geology Lab 1 150 2 128.52 C 16 Geology Lab 2 375 2 128.52 C 17 Cisco Lab. 375 0 0 C 18 Applied Electricity Electronics Lab 150 0 0 C 19 Materials And Structures Lab. 150 2 128.52 C 20 Metal Workshop 150 2 128.52 C 21 Bio-Chemistry Lab. 150 15 963.9 B 22 Physiology Lab. 150 15 963.9 B 23 Doctor’s Office (Medical lab) 25 1 64.26 C 24 Injection Room (Medical lab) 25 1 64.26 C The distribution of the categorized water use is shown in Figure 1. Four laboratories were selected based on the class and respective laboratory courses on the campus. The selected
  • 5. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME laboratories are Industrial chemistry lab, (ICH), Biochemistry lab, (BCH), Medical lab (MED) and Water and environmental lab (WE).
  • 6. 107 Figure 1: Water Consumption/Lab-Session 2.5 Materials and Experimental Protocols The materials required to carry out the above exercise include; Photometer Test tubes Plastic container or kegs with air tight cover Measuring cylinder Distilled water 70% ethanol Cotton wool pH meter Turbidity Meter Reagents Stop watch hose 2.5.1 Sterilization of Materials All glass wares were thoroughly washed with detergent solution, rinsed with several changes of distilled water and subsequently allowed to drain, after which they were sterilized or disinfected with 70% ethanol. The working bench was also swabbed with 70% alcohol to have a sterile environment. 2.5.2 Labelling of samples Samples collected were labelled according to the tabulated codes shown in Table 2 below to avoid mix up of different samples. Table 2: Labeling of Samples LABORATORY INFLUENT LABELLING EFLUENT LABELLING Sample 1 Sample 2 Sample 1 Sample 2 Industrial chemistry lab ICH-IS1 ICH-IS2 ICH-ES1 ICH-ES2 Bio-chemistry lab BCH-IS1 BCH- IS2 BCH-ES1 BCH-ES2 Micro-biology lab MCB-IS1 MCB-IS2 MCB-ES1 MCB-ES2 Health centre HC-IS1 HC-IS2 HC-ES1 HC-ES2
  • 7. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 108 2.5.3 Collections of Samples Utmost and scrupulous care was taken in the collection of the water and waste water samples to ensure the samples are representative of the water and waste water desired to the examined and to avoid accidental contamination of the samples. To collect influent samples, the outlet of the running tap of the laboratories were disinfected using 70% ethanol with the aid of a swab and was allowed to run for at least five minutes before taking the sample into an air tight container or kegs in order to stabilize its temperature and to carry out analysis on the influent samples of each laboratory. The tap water samples were transported to the water and environmental laboratory for analysis in ice packs. For the collection of effluent samples, the sewage drain of the laboratories was located and samples were taken with tapping the sewage channel or drain of all sinks and wash hand basins in all the laboratories. This was channeled into an air tight container or big keg of about 50litres with a hose in order to stabilize its temperature and was refrigerated to carry out analysis on the second day. The container or kegs should not be filled above 90% to avoid spillage during transportation. The samples were transported in ice packs to the water and environmental laboratories for analysis. 2.6 Physico-chemical Analysis of Influent and Effluent Physical observations were made of the colour and odour of the samples while other physical properties, pH, temperature and turbidity were measured with pH meter, thermometer and turbidimeter respectively. In determining the turbidity of the sample, a two part calibrated turbidity tube was used with calibrations from 5-25 turbidity units. The joined tubes were held over a white paper, while slowly pouring the water sample into the tube until the black cross at the bottom was no longer visible. At this point the reading was taken from the side of the tube as the turbidity value of the water sample. Alkalinity, Total Hardness and the presence of Chlorine (DPD), Phosphate, Zinc, Nitrate, Nitrite, Calcium were determined in the influent. The Automatic Wavelength Photometer was used in carrying out this exercise. Standard procedures were followed with special attentions given to various notes for each of the above parameters as specified in the relevant manual. Analyses of the samples were carried out at the Water and Environmental laboratory in Osun State University and RUWESA in Osun State Government Secretariat in Abere, Oshogbo. 2.7 Estimation of Recyclability The effluent is further analyzed for toxic/hazardous chemicals to serve as recyclability indicators. The additional tests were for Ammonia, Chromium (Hexa/6+), Copper, Aluminum, Silica, Sulphate, Molybdate and Bromine total. These tests are for advanced analysis of waste water sample from laboratories and industries which help in the detection of toxic materials and recyclability of waste water sample. The effluent hazardous chemical levels determined. Differences in the influent and effluent were estimated as percentages of influent. These are to serve as treatment levels required for recyclability. 3.0 RESULTS ANALYSIS AND DISCUSSION Results of the various physical and chemical waste water quality parameters for both influent and effluent are obtained for further analysis. Analysis of the differences in the wastewater quality parameters between the influent and effluent materials from each laboratory are determined so as to indicate what has been lost through the activities in the laboratories. The differences are presented in percentage forms in Table 3, so as to evaluate degrees of degradations experienced in the use of the influent water into each laboratory. Items 1 to 8 deal with the physical qualities of both the influent
  • 8. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME and effluent waste water. The chemical properties are shown in items 9 to 23. The results obtained for additional toxic materials are indicated in Table 3. 109 3.1 Physiochemical evaluations The influent and effluent Physicochemical Parameters are shown in Table 3 which indicates the percentage differences in each laboratory. Results of the Toxic and Recyclability Indicator of Laboratory Effluent Table 3: % Difference Between Influent And Effluent Pysico-Chemical Parameters S/N Parameter Unit (%) Difference / Laboratories Bio Chem Ind Chem Medical Water Env 1 Colour TCU 15 15 15 15 2 Odour - - - - 3 Taste - - - - 4 pH 37 23 -42 9 5 Temperature OC 12 25 3 6 6 Conductivity μS/cm - - - -119 7 Turbidity NTU - - - - 8 T.D.S Mg/L - - -221 -141 9 O.R.P Mv -53 -129 -66 -59 10 Chloride Mg/L -18 33 50 47 11 Total Hardness Mg/L -602 -422 -140 12 Calcium Hardness Mg/L 17 90 -650 -471 13 Magnessium Hardness Mg/L -723 - -362 -86 14 Total Chlorine Mg/L -189 96 79 29 15 Free Chlorine Mg/L -280 100 -250 60 16 Total Alkalinity Mg/L -574 -448 -88 -79 17 Nitrite(NO2) Mg/L 31 73 8 2 18 Nitrate(NO3) Mg/L Inconclusive 43 38 1 19 Arsenic Mg/L - - - - 20 Iron Mg/L 100 100 96 -12 21 Manganese Mg/L 96 84 8 28 22 Fluoride Mg/L -496 69 60 13 23 Zinc Mg/L -1900 -539 -15 13
  • 9. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. The % differences in the Physical 103-113 © IAEME Parameters could not be ascertained for colour effluent. Similarly, percentage differences for odor and taste than 15 (15) for both the influent and effluent influent and effluent only referred to as unobjectionable unobjectionable, (UO), while Turbidity % differen greater than 5, and therefore these differences cannot be determined. Conductivity values of some laboratories are greater than the photometer range. There is no arsenic from the analysis carried out hence the percent differences The percent differences in the chemical para levels of treatments required for recyclability. Activities in the Biochemistry lab presented the worst situation in the areas of total hard requirements as their percent differences are more of less between 500% and 720%. From the presentations of the total chlorine, free chlorine, this same laboratory needs more attention in disinfection of the waste water [6], [7] Figure 2: %Difference Between Influent and Effluent The percent difference between influent water and effluent waste water presents the worst situation for Zinc in Table 3, getting to as great as 1900% and 539% for Biochemistry lab and Industrial Chemistry lab respectively. The other laboratories for the nitrate level for Biochemistry lab was inconclusive. Zinc levels, although there is no health impact currently associated with it, it should not be allowed to keep rising in a situation of recyclability. Results of tests of the parameters considered to be toxic undertaken only for the effluen waste water is shown in Table 4 Organization of Nigeria, [14] and [15 water [14], [2] and illustrated in Figure need to be watched, especially in the Biochemistry and Industrial Chemistry laboratories as they are currently greater than the permissible levels Although [14] did not specify safe limits for some parameters, caution should be exercised in allowing their concentrations to keep building up. There is therefore need for evaluation of these parameters indicted at the influent level so as to monitor any development. 110 and were not determined. parameters are presented in Figure 2 hardness, magnesium hardness, total alkalinity and fluoride 7]. , had differences lower than 20%. Results There is therefore the need aste 4. These are compared with the guidelines of the Standard 4] 15], for quality assessment of good industrial water/ safe drinking 3. It can be observed that Chromium and Sulphate levels that [1], [4]. They need to be treated before recycling. d – 6308 (Print), as it is less difference is total hardness meters to indicate the ness, to monitor the effluent . .
  • 10. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. Table 4: Results of the Toxic S/N Waste Water Recyclability Indicator Unit 1 Ammonia Mg/L 2 Phosphate Mg/L 3 Chromium Mg/L 4 Alluminuim Mg/L 5 Silica Mg/L 6 Sulphate Mg/L 7 Molybdate Mg/L 8 Total Bromine Mg/L 9 Hexavalent Chromium Mg/L 10 Total Copper Mg/L 11 Free Cooper Mg/L 12 Sulphide Mg/L 13 Chlorinedioxide Mg/L and Recyclability Indicator of Laboratory Effluent Laboratories SON BCH ICH MED WE SON Max Figure 3: Toxic and Recyclability Indicator of Lab Effluent
  • 11. !##$ 103-113 © IAEME 111 permitted 0.32 0.04 0.46 0.15 1.00 1.18 0.79 0.08 0.02 6.00 0.14 0.10 0.03 0.01 0.05 0.11 0.10 0.06 0.04 0.20 4.00 175 170 56 80 100 0.56 0.08 0.04 0.02 0.13 0.28 0.23 0.01 0.70 0.00 0.01 0.00 0.58 0.00 0.00 0.00 0.12 0.08 0.03 0.11 0.16 0.04 0.01 0.02 – 6308 (Print), Remark Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable Recyclable
  • 12. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 4.0 CONCLUSIONS AND RECOMMENDATIONS 112 Water use in these laboratories has been estimated to 64.26 Liters/tap. Out of a total of 24 laboratories on the campus 4 laboratories were selected for this exercise based on the gradation of water consumption rates and laboratory courses on the campus. Detailed study were undertaken for the selected laboratories, Biochemistry lab, (BCH); Industrial Chemistry lab, (ICH); Medical lab (MED); and Water and Environmental lab (WE). Results of the differences in the water quality parameters for the influent water and the effluent waste water will indicate the levels of treatments required for recyclability in each laboratory. It can be concluded that the effluent from the Biochemistry lab requires the greatest attention on treatment level scale. Some of the areas that need much attention are in the areas of total hardness, magnesium hardness, total alkalinity, fluoride, total chlorine, free chlorine, and zinc. Although zinc is not regarded as having associated health impact, the rate of its deposit during the use of water in the Biochemistry and Industrial Chemistry practical sessions is of concern. The rate was 1900% and 539% when compared with the influent values at the beginning of each practical, while rates for the other two labs were below 20%. There is therefore the need to monitor the Zinc levels to avoid its being allowed to keep rising in a situation of recyclability. Since the results for the nitrate level for Biochemistry lab was inconclusive, there is need to further investigate this. The Chromium and Sulphate levels should be further monitored as they are currently greater than the SON permissible levels within the Biochemistry and Industrial Chemistry labs. We are recommending that treatment facility should give further attention to these parameters. It is also recommended that the influent water be analyzed to determine levels of the 13(No) parameters indicated in Table 4 so as to be able to monitor any development at effluent level. Further works should also be carried out to determine the effluent levels, whose values could not be picked in the present study, for silica and molybdenum salts Generally, although SON did not specify safe limits for some parameters, caution should be exercised in allowing their concentrations to keep building up. REFERENCES [1] Atici, T., Ahiska, S., Altindag, A., Aydin, D. 2008: Ecological effects of some heavy metals (Cd, Pb, Hg, Cr) pollution of phytoplanktonic algae and zooplanktonic organisms in Saryyar Dam Reservoir in Turkey. African J. Biot. 7(12): 1972–1977. [2] Eaton, Andrew D, 2005: Standard methods for the examination of water and wastewater. American Public Health Association; American Water Works Association; Water Environment Federation. Publisher: Washington, D.C. : APHA-AWWA-WEF, 2005. [3] Google Earth, 2013: Accessed November, 8, 2013. [4] Hui, K. S., Chao, C. Y. H., Kot, S. C. 2005: Removal of mixed heavy metal ions in wastewater by zeolite 4A and residual products from recycled coal fly ash. Journal of Hazardous Materials B, 127, 89-101. [5] Mbajiorgu, C.C., 2003: A water quality study of Ulasi river at selected locations. 29th WEDC International Conference Abuja, Nigeria, 2003. [6] Metcalf, Leonard and Harrison P. Eddy, 1922: Sewerage and Sewage Disposal: A Textbook. New York: McGraw-Hill. [7] Metro Vancouver, 2011: Regional Growth Strategy. July 29, 2011 Retrieved February 14, 2013.
  • 13. International Journal of Civil Engineering and Technology (IJCIET), ISSN 0976 – 6308 (Print), ISSN 0976 – 6316(Online), Volume 5, Issue 9, September (2014), pp. 103-113 © IAEME 113 [8] Nasrullah R. N., B. Hamida, I. Mudassar and M. I. Dur-rani, 2006: “Pollution Load in Industrial Effluent and Ground Water of Gadoon Amazai Industrial Estate (GAIE) SSWABI, NWFP,” Journal of Agricultural and Biologi-cal Science, Vol. 1, No.3, 2006, pp. 18-24. [9] Neal, C. and A. J. Robson, 2000: A summary of river water quality data collection within the Land-ocean interaction study: core data for Eastern UK Rivers draining to the North Sea. Science of the Total Environment, 251/252, 585-665. [10] Oginni, F. A., 2013: Variations in the Water Quality of an Urban River in Nigeria. Scientific Research Publishing on Journal of Computational Water, Energy, and Environmental Engineering, 2013, 2, 81-91 doi: 10.4236/cweee.2013.22B014 Published Online April 2013. http://www.scirp.org/journal/cweee. [11] Ojoawo, S. O., F. A. Oginni, M. Jolayemi and O. J Adenrele, 2014: Domestic Wastes Generation Pattern and Composition in Osogbo and Environs, Osun State, Nigeria. International Journal of Civil Engineering (IJCE). ISSN(P): 2278-9987; ISSN(E): 2278-9995; Vol. 3, Issue 4, July 2014, 47-56. [12] Schmitt, Eric (2000): Everglades Restoration Plan Passes House, With Final Approval Seen, The New York Times, October 20, 2000, p. 1. [13] Snyder G.H. and J. Davidson, 1994: Everglades Agriculture: Past, Present and Future in Everglades: The Ecosystem and its Restoration, Steven Davis and John Ogden, eds. (1994), St. Lucie Press. ISBN 0-9634030-2-8. [14] Standards Organizations of Nigeria, (SON), 2007: Nigerian Standard for Drinking Water Quality. Nigerian Industrial Standard NIS 554: 2007. [15] World Health Organisation, WHO, 2000: WHO guidelines for drinking water quality training pack. Rome: WHO, 2000. [16] H J Surendra and Paresh Chandra Deka, 2012: Effects of Statistical Properties of Dataset in Predicting Performance of Various Artificial Intelligence Techniques for Urban Water Consumption Time Series. International Journal of Civil Engineering Technology (IJCIET). ISSN(P): 0976-6308; ISSN(E): 0976-6316; Vol. 3, Issue 2, 2012, 426 – 436. [17] Dr. P. Mariappan, 2012: Wastewater Management in a Dwelling House- A Case Study. International Journal of Civil Engineering Technology (IJCIET). ISSN(P): 0976-6308; ISSN(E): 0976-6316; Vol. 3, Issue 2, 2012, 16 - 24. [18] R Radhakrishanan and A Praveen, 2012: Sustainability Perceptions on Wastewater Treatment Operations in Urban Areas of Developing World. International Journal of Civil Engineering Technology (IJCIET). ISSN(P): 0976-6308; ISSN(E): 0976-6316; Vol. 3, Issue 1, 2012, 45 - 61.