2. Before taking a blood sample, it is extremely
important to be fully familiar with the
collection system being used.
It does not just make an unprofessional
impression to fiddle about with the blood
collection equipment, it will make the
patient feel increasingly anxious, which will
have a negative influence on the condition
of the veins.
4. Vein Selection: Priority List
1. Median cubital/antecubital veins
2. Dorsal hand veins
3. Foot veins
4. Subclavian vein
5. Femoral vein
5. Pre preparation
Fast (if required)
Smile to the patient and let him/her feel
comfortable
Collect patient’s information (Full name,
date of birth)
Position the patient on a chair or a bed
Wash your hands, wear gloves.
Select the suitable site for venipuncture
6. Procedure
1. Prepare the appropriate syringe or
needle
2. If multiply specimens are to be
collected, it is better to use butterfly
needle
3. Put the tourniquet on (not too tightly and
do not leave it longer than 1 minute)
4. Sterilize the area in a circular motion.
7. 5. Ask the patient to make a fist
6. Stretch the skin next to the venipuncture
area (be careful not to touch the site
itself)
7. The needle should be 10-20 degree
angle
8. After finishing filling the needed tubes,
release the tourniquet
9. Remove the needle from patient’s body
10. Put a gauze immediately on the
puncture site and hold adequate
pressure to avoid formation of a
hematoma
8.
9.
10. Factors leading to difficult vein
condition
Anxiety
Delicate veins (children / woman)
Cold
Poor hydration
Vasoconstriction of veins
Pre-shock or shock
Thin veins
Brittle veins
Repeatedly punctured veins
Long term treatment with steroids
Sclerosed veins
Cachexia
Rolled veins