Poster for CIAT 2009 Knowledge Sharing Week

1. CIAT Phenotyping Platform:
Aiming at improving eco-efficiency of crops in the changing global climate
Jagadish Rane, Idupulapati Rao, Manabu Ishitani, Cesar Martínez and Joe Thome
Centro Internacional de Agricultura Tropical (CIAT), A. A. 6713, Cali, Colombia (E-mail: j.rane@cgiar.org)
RATIONALE OBJECTIVES
To meet global food demand, it is essential to improve crop and livestock productivity in To facilitate high throughput phenotyping under field and controlled environment with
agroecosystems frequently affected by abiotic stresses and those which are prone to adverse
effect of climate change. Problems associated with consumption of water and fertilizer needs to Automation, improved precision, efficient management of stress environment
be solved to ensure eco-efficiency. Agricultural research interventions aiming at Water Use Precision in characterizing plant responses and growth environment
Efficiency (WUE) and Nitrogen Use Efficiency (NUE) of crop and forage components need to be Accessible noninvasive advanced instruments and methods
explored and implemented to accomplish the task.
To facilitate development of agronomic ally superior crop variety with a stack of
The recent advances in molecular biology have generated more hopes to overcome abiotic Desirable genes associated with traits
constraints as techniques and tools are more robust now than ever before to further explore the Traits associated with abiotic stress tolerance
genome with greater efficiency and with increasing pace. However, understanding the genome
function and its application for crop and forage improvement is the bottle neck. Hence
phenomics is crucial to realize the benefits of advanced biological knowledge for sustaining
ecosystems while enhancing crop and livestock production in the tropics. CIAT researchers
SCALED UP CAPABILITIES
are improving the phenotyping capabilities to improve eco-efficiency of its mandated crop and TO EVALUATE GENE TECHNOLOGY IN RICE
forage components. Here we show the recent advances in phenotyping rice as a case study.
Drought Screening strategy: Upland Rice
Transgenic Events
TARGET TRAITS AND CROPS SCREENHOUSE
FIELD
Seedling stage
Vegetative stage
Traits Nitrogen High
Water Use Acid Soil Reproductive stage
Use Temperature
Crops Efficiency Tolerance Our major efforts
(generic permit approved)
Efficiency Tolerance Detailed study
Promising
transgenic
lines
Bean
Cassava
Boom system for precise irrigation
Forage Network of probes for soil moisture profile
(Brachiaria)
Rice Simulation of natural upland rice environment
& rainout shelter
VISUALIZED PHENOTYPING PLATFORM
Conventional Genomics
Instruments Breeding
Gene/Molecular Markers for MAS Genotyping
Base material RIL, NIL, CSSL
Mutants
Mapping population, Transgenic
& Product RILs, CSSL, Transgenic,
Phenotyping method & tools
Promising lines
Sources:
CIAT, CG, International
Identified superior Advanced Laboratories, Phenotype data
NARS
Relative Units
parental lines Gene function
for specific traits Proof of concept
Manual Phenotyping process Automation PHENOMICS
FIELD PHENOTYPING TRAIT PHENOTYPIG
Controlled environment and
automated phenotyping
(http://www.lemnatec.com/)
Well managed stress environment Controlled environment
Natural conditions Plant Precise control of
Less or well characterized soil heterogeneity Growth Temperature, humidity and radiation
Precise quantification of stress Environment Automated and precise delivery of
water
Automatic Weather Station
nutrient
Phenotyping for Identification of traits and
NDVI sensor IR Image revealing Traits , Techniques for high throughput screening
yield, growth and proven traits
canopy temperature Gene discovery
associated with targeted stresses, Techniques, Validation Root traits
Multilocation Trials(GxE) Superior parental lines
Remote sensing (NDVI), Lemna Tec
Automation,
IR Imaging, Automation
RFID and remote sensors based techniques
Noninvasive (SPAD) High throughput Root Scanners
Image based techniques
Barcode identifiers, PDA Lemna Tec
Data Acquisition, Processing Instant data processing system
Remote Access to servers
Data Management (LIMS)
Confined field Biosafety Screenhouse/Greenhouse Lemna Tec automated
PDA with barcode reader + Biosafety Risk Avoidance phenotyping system
User friendly data acquisition For Gene Technology (http://www.lemnatec.com/)
SOP