Poster54: CIAT phenotyping platform: Aiming at improving eco-efficiency of crops in the changing global climate
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Poster54: CIAT phenotyping platform: Aiming at improving eco-efficiency of crops in the changing global climate

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Poster for CIAT 2009 Knowledge Sharing Week

Poster for CIAT 2009 Knowledge Sharing Week

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  • 1. CIAT Phenotyping Platform: Aiming at improving eco-efficiency of crops in the changing global climate Jagadish Rane, Idupulapati Rao, Manabu Ishitani, Cesar Martínez and Joe Thome Centro Internacional de Agricultura Tropical (CIAT), A. A. 6713, Cali, Colombia (E-mail: j.rane@cgiar.org) RATIONALE OBJECTIVES To meet global food demand, it is essential to improve crop and livestock productivity in To facilitate high throughput phenotyping under field and controlled environment with agroecosystems frequently affected by abiotic stresses and those which are prone to adverse effect of climate change. Problems associated with consumption of water and fertilizer needs to Automation, improved precision, efficient management of stress environment be solved to ensure eco-efficiency. Agricultural research interventions aiming at Water Use Precision in characterizing plant responses and growth environment Efficiency (WUE) and Nitrogen Use Efficiency (NUE) of crop and forage components need to be Accessible noninvasive advanced instruments and methods explored and implemented to accomplish the task. To facilitate development of agronomic ally superior crop variety with a stack of The recent advances in molecular biology have generated more hopes to overcome abiotic Desirable genes associated with traits constraints as techniques and tools are more robust now than ever before to further explore the Traits associated with abiotic stress tolerance genome with greater efficiency and with increasing pace. However, understanding the genome function and its application for crop and forage improvement is the bottle neck. Hence phenomics is crucial to realize the benefits of advanced biological knowledge for sustaining ecosystems while enhancing crop and livestock production in the tropics. CIAT researchers SCALED UP CAPABILITIES are improving the phenotyping capabilities to improve eco-efficiency of its mandated crop and TO EVALUATE GENE TECHNOLOGY IN RICE forage components. Here we show the recent advances in phenotyping rice as a case study. Drought Screening strategy: Upland Rice Transgenic Events TARGET TRAITS AND CROPS SCREENHOUSE FIELD Seedling stage Vegetative stage Traits Nitrogen High Water Use Acid Soil Reproductive stage Use Temperature Crops Efficiency Tolerance Our major efforts (generic permit approved) Efficiency Tolerance Detailed study Promising transgenic lines Bean Cassava Boom system for precise irrigation Forage Network of probes for soil moisture profile (Brachiaria) Rice Simulation of natural upland rice environment & rainout shelter VISUALIZED PHENOTYPING PLATFORM Conventional Genomics Instruments Breeding Gene/Molecular Markers for MAS Genotyping Base material RIL, NIL, CSSL Mutants Mapping population, Transgenic & Product RILs, CSSL, Transgenic, Phenotyping method & tools Promising lines Sources: CIAT, CG, International Identified superior Advanced Laboratories, Phenotype data NARS Relative Units parental lines Gene function for specific traits Proof of concept Manual Phenotyping process Automation PHENOMICS FIELD PHENOTYPING TRAIT PHENOTYPIG Controlled environment and automated phenotyping (http://www.lemnatec.com/) Well managed stress environment Controlled environment Natural conditions Plant Precise control of Less or well characterized soil heterogeneity Growth Temperature, humidity and radiation Precise quantification of stress Environment Automated and precise delivery of water Automatic Weather Station nutrient Phenotyping for Identification of traits and NDVI sensor IR Image revealing Traits , Techniques for high throughput screening yield, growth and proven traits canopy temperature Gene discovery associated with targeted stresses, Techniques, Validation Root traits Multilocation Trials(GxE) Superior parental lines Remote sensing (NDVI), Lemna Tec Automation, IR Imaging, Automation RFID and remote sensors based techniques Noninvasive (SPAD) High throughput Root Scanners Image based techniques Barcode identifiers, PDA Lemna Tec Data Acquisition, Processing Instant data processing system Remote Access to servers Data Management (LIMS) Confined field Biosafety Screenhouse/Greenhouse Lemna Tec automated PDA with barcode reader + Biosafety Risk Avoidance phenotyping system User friendly data acquisition For Gene Technology (http://www.lemnatec.com/) SOP