Mushroom spawn production

1. Rofp ljp pTkfbg
k|ljlw
Mushroom Production Technology
Jflzi7 cfrfo{
/fli6«o jfnL /f]u lj1fg cg';Gwfg s]Gb|
V'fdn6f/, nlntk'/

2. Rofp lap -:kg_
 Rofpsf] ljpnfO{ :kg -spawn_ elgG5 .
 :kg eGgfn] ;J:6«]6df dfO{;]lnodsf] j[l¢ / ljsf; u/fpg'
xf] h'g Rofp pTkfbgsf] nflu ljpsf] ?kdf k|of]u ul/G5
.
 ;fdfGo efiffdf Rofpsf] :kg pRr jg:kltsf] cn}lËs ljp
;+u ;dt'No x'G5 .
 :kg÷Rofpsf] ljp pTkfbg eGgfn] hLjf0f' /lxt cj:yfdf
rflxPsf] Rofpsf] dfO{l;lnodnfO{ lgd{nLs[t pko'Qm
;J:6«]6df j[l¢ / ljsf; u/fpg' xf] .
 :kg pTkfbgsf nflu lgd{nLs[t k|of]uzfnf / bIf
hgzlQm rflxg] ePsfn] of] Pp6f hl6n k|s[of xf] .

3. Rofpsf] ljp jgfpg] k|of]uzfnfsf nflu
cfjZos ;fdfu|Lx?M
 c6f]Sn]e -hLjf0f'z'GoLs/0f ;fdfu|L_
 hLjf0f'ljlxg jftfj/0fsf nflu n]ldgf/ Po/kmnf]
 O{Gs'j];g sf]7f, O{Gs'j]6/
 /]lkm|h]/]6/
 Unf;j]o/ -6]:6 6o'j, k]l6«l8;_
 ldl8of sDkf]g]G6x? -cu/, dfN6 PS:6«ofS6_
 cGo /;fog -lhK;d, rs_
 ;J:6««]6sf ;fdfu|L -ux‘F‘'sf] ljp, sf7sf] w'nf]_
 z'¢ sNr/ jf tfhf km'|6j8L
 :kgsf efF8fx? -jf]tn jf Knfl:6ssf] emf]nf_

4. Rofpsf] ljp jgfpg] k|of]uzfnfsf nflu cfjZos
;fdfu|Lx?M
c6f]Sn]
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cf]eg
n]ldgf/
Po/kmnf]
O{Gs''

5. Unf;j]o/ -6]:6 6o'j, k]l6«l8;_

6. ldl8of sDkf]g]G6x? -cu/, dfN6 PS:6«ofS6,
lhK;d, rs_

7. ;J:6««]6sf ;fdfu|L -ux‘F‘'sf] ljp, sf7sf]
w'nf]_

8. z'¢ sNr/ jf tfhf km'|6j8L

9. l6:o' sNr/M
tfhf Rofpsf] s'g}klg efu k|of]u ug{ ;lsg] ePtfklg vf;u/L
5ftfsf] tNnf] efu hxfF 8fF7;+u jLhkq hf]l8Psf] x'G5 ;j}eGbf
jl9 pko'Qm x'G5 .
 RofpnfO{ kfgLn] /fd|f] ;+u wf]O{ 6f;LPsf] w'nfd}nf x6fpg
%) Ü O{yfgf]ndf ;kmf ug]{ .
 ;ªs|d0fjf6 ;'/lIft /fVgsf] nflu gd'gfnfO{ ! K|fltzt ;f]l8od
xfO{kf]Snf]/fO{8 emf]ndf #)—^) ;]s]G8sf] nflu 8'jfpg'
kb{5 .
 lgd{lns[t :Sofnk]n Jn]8n] nDjfO{jf6 b'O{ efudf sf6L
lgd{lns[t ;'O{/f]n] cfGtl/s tGt'sf] Pp6f 6's|f lems]/ Jn6/
k]k/df /flv ;'Vvf u/fP/ t'?Gt sNr/ ldl8ofdf /fVg' kb{5 .
 O{gf]s'n]6]8 cu/ :nfG6nfO{ @% l8=;]= tfks|ddf

11. Procedure for PDA Preparation:
Composition: Potato: 200 gm, Dextrose: 20 gm, Agar: 20 gm,
Water: 1 ltr
Half boil 200 g sliced potatoes in 500 ml distilled water for
10-15 min.
Filter through cheese cloth, and the decoction is kept in a
beaker
Mix agar and dextrose and boil to dissolve in rest 500 ml
water with continuous stirring
 Mix solution with continuous stirring and made them
homogenous.
Volume up to 1 ltr is adjusted with distilled water
Autoclave 15 min at 121°C and 15 p.s.i.
Dispense 20-25 ml portions into sterile 15 × 100 mm petri
dishes.

12. :kf]/ sNr/M
hLjf0f' /lxt cj:yfdf :j:y Rofpsf] km'|6j8Ljf6 df]gf] jf
dN6L :kf]/ ;+sng ul/G5 .
To;kl5 k]l6«l8;df /flvPsf] lkmN6/ k]k/dfyL /flvG5
/ ljs/n] 5f]lkG5 .
!, @ lbgdf pQm lkmN6/ k]k/dfyL jfVnf] :kf]/df;
x'G5 To; kl5 ljs/ x6fP/ k]l6«l8;sf] 9Ssgn] 5f]k]/
k|of]u gu?Gh]n /]lkm|h]/]6/df /fVg' kb{5 .
 df]gf]:kf]/ sNr/M
 dlN6 :kf]/ sNr/

13. ;jsNr/
 Tfof/ ul/Psf] 6]:6 6o'j sNr/nfO{ :kg
O{gf]s'ndsf] ?kdf k|of]u ug'{ cufl8 w]/}j6f
:nfG6df ;jsNr/ ug{ ;lsG5 .
 :kgsf] ;fgf] c+z ;fjwfgLk'j{s cu/ :nfG6df
:yfgfGt/0f u/]/ sNr/ pdf{g ;lsG5 .
:kg sG6]g/M
Tffk k|lt/f]lw / pko'Qm 9Ssg ePsf] x'g' kb{5, klxn]
l;;fsf] jf]Qnx? K|fof]udf cfpg] ePtfklg cfhef]nL tfk
k|lt/f]lw Knfl:6ssf] emf]nf k|of]u ul/G5 .
Wf]/} 7'nf] :kg sG6]g/sf] k|of]u ubf{ lj;+s|d0f

14. :kg ;J:6«]6 / o;sf] tof/LM
;J:6«]6 5gf}6 ub{f :yflgo :t/df ;xh} pknJw x'g]
/ dfO{l;lnodsf] /fd|f] ;+u ljsf; x'g] x'g kb{5 .
l6:o' jf :kf]/ jf6 ljsf; ePsf] z'å sNr/nfO{
pko'Qm ;J:6«]6df O{gf]s'n];g u/fO{G5 .
;J:6«]6nfO{ /fd|f] ;+u kvfn]/ lehfpg] / dfyL
pq]sf] bfgfnfO{ ;fjwfgLk'j{s x6fpg] .

15.  bfgfnfO{ /fd|f] ;+u kvfn]/ s/Lj !% ln= kfgLdf !)
s]=hL= ;J:6«]6 /fvL xfkm JjfO{n ug{' kb{5 .
 bfgf g/d t/ gk'm6]sf] x'g' kb{5 cGoyf w]/} g/d /
6fl;g] x'G5 .
 bfgfsf] kfgL tsf{P/ lr;f] x'g lbg' kb{5 .
 rssf] w'nf]÷r'gf -Sofnl;od sfjf]{g]6_ ^
u|fd÷s]=hL ljp / lhK;d -Sofnl;od ;Nkm]6_ !@
u|fd÷s]=hL ljp sf b/n] Psgf; ;+u ld;fpg' kb{5 .
 Sofnl;od ;Nkm]6n] bfgfx? Ps cfk;df 6fl;gjf6
/f]Sb5 eg] Sofnl;od sfjf]{g]6n] bfgfsf] lk=Pr=
dfg sfod ug{ dbt ub{5 .

16.  jf]tn jf Knfl:6s emf]nf b'O{ ltxfO{ dfq e/]sf] / s6g
Knun] jGb u/]sf] x'g' kb{5 .
 pQm jf]tn jf Knfl:6s emf]nf nfO{ c6f]Sn]e leq !@!
l8=;]= tfks|d, !% kfpG8 k|];/df s/Lj !%–#) ldg]6
;Dd lj;+s|ldt ug'{ kb{5 .
 c6f]Sn]ejf6 lems]/ ;J;6«]6nfO{ lr;f] x'g lbg' kb{5 .
 lr;f] ePkl5 emf]nf jf jf]tnnfO{ O{gf]s'n]6 ug'{ kb{5
.
 ;j} O{gf]s'n]6]8 sG6]g/nfO{ @%± ! l8=;] tfks|ddf
!) !% lbgsf] nflu O{Gs'j]6 ug'{ kb{5 .
 bfgfnfO{ v's'nf] /fVg #, $ lbgdf sG6]g/nfO{

17. KnflG6Ë :kgsf] tof/LM
Pp6f dft[ sNr/ 6o'jf6 ^ j6f ;jsNr/ ug{ ;lsG5 .
Pp6f ;jsNr/jf6 !) j6f dft[:kg jf]tn tof/ ug{
;lsG5 .
Pp6f dft[:kg jf]tnjf6 @) j6f KnflG6Ë :kg tof/
ug{ ;lsG5 .
Rofp lahgsf] u'0f:t/M
 u'0f:t/Lo :kgsf] Rofp pTkfbg k|s[ofdf
dxTjk'0f{ e'ldsf /x]sf] x'G5 .
 Rofpsf] lahg /fd|f] x'g'kb{5 . h;df !)–!% lbg
k'/fgf] w]/} dfly ;Dd /];f (mycelium)

19. Rofpsf] ljhg KoflsË /
9'jfgL