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Chapter 3
BASIC REQUIREMENTS FOR
SENSORY EVALUATION
Objectives:
 At the end of this chapter, you will be able to:
 Identify and determine the taste panel area.
 Determine and understand the basic
requirements during sensory lab.
 Determine the factors to choose and train
panelist.
 Determine the factors influencing sensory
measurements.
Sensory laboratory
 Pleasing natural shades and
maintenance of comfortable
temperature and humidity
conditions of the laboratory
set up are desirable.
 Test room should be separated
by suitable distance from
congested are because &
noise and the opportunity this
would provide for unwanted
socializing.
Briefing room
Panel room
Preparation room
Sensory Laboratory Set Up and
Equipment
 The physical setting must be designed so as to
minimize the subject's biases, maximize their
sensitizing, and eliminate variable which do not
came from the product themselves.
 The test area should be centrally located, easy to
reach and free of crowding and confusion, as
well as comfortable, quite, temperature
controlled and free from odour & noise.
TASTE PANEL AREA
1. The testing booths are located should be separated from sample
preparation room and wash room and store by a complete partition.
2. A temperature of about 20°C and RH of 62% in the testing room in
considered to be ideal.
3. The booths may be fairly simple or very elaborate, depending on the
funds available.
4. Many laboratories have a sink and tap built into each booth for
expectoration and to provide water for rinsing.
5. For descriptive analvsis and panel training sessions a round table that
permits discussion is required.
6. The color of the booths should not influence the appearance of the
product being judged. An off-white or light gray color is usually
recommended.
LIGHTING
 Lighting should be uniform and should not
influence the appearance of the product to be
tested. The type of light used should be carefully
chosen if color and appearance are important
factors to be judged, since many fluorescent
lights distort color.
 A common feature of many panel booths is a choice of
red, green, and / or blue lighting at low intensity obtained
through the use of colored bulbs or special filters. The
lights are used to mask visual differences between
samples in differences tests calling for the subject to
determine by taste ( or by feel, if appropriate) which
samples are identical.
TESTING SCHEDULE
 The time of day that tests are run influences
results. Although this cannot be controlled if the
number of tests is large, late morning and mid
afternoon are generally the best times for testing.
The type of preparation equipment located in the
sample preparation area depends on the products
being tested.A well-equipped kitchen is a good
start, with specialized equipment being added
according to need.
 The preparation area should have a good
ventilation system for removal of cooking odors.
Sufficient counter space for serving and
assembling samples for presentation is an
important requirement (Fig. 3).
PREPARATION ROOM &
SAMPLES PREPARATION
• A laboratory bench flush with the hatches so that samples
trays will side through. The hatch on the service counter
should preferably be constructed in such a manner that
there shall be no recognition of individual or either side
of partition.
• Benches, kitchen range, ovens, etc. for preparation.
• Refrigerator and freezer for storage of samples.
• Storage for glassware, dishes, glasses, trays, etc.
• Dishwashers, disposers, trash compactors, wastebaskets,
sinks, etc.
• Frozen storage for panel member treats, if used
• Large garbage containers for quick disposal of used
product, etc.
Preliminary testing
 Preliminary testing is usually necessary to
determine the method of preparation of the
product.
 All the various factors, such as time and
temperature of thawing and cooking, amount
of water and size of pot for boiling, and time
and speed of blendjng, should be
predetermined and kept constant throughout
tests on the product.
 The_preparation method should not impart
any foreign tastes or odors to the product.
 Panelists are influenced by irrelevant
characteristics of the samples. Because of this,
every effort should be made to make the samples
from different treatments identical in all
characteristics except the one being judged. It is
sometimes necessary to grind dice, chop, or
puree the samples to obtain uniformity.
 However, Kefford and Christie (1960) found that
judges prefer foods in their normal state. When
conducting acceptance or preference tests, it may
be better to present one sample at a time rather
than grind or puree the product to mask the
differences.
DILUTION AND CARRIERS
 Most foods are served in the way in which
they are normally consumed.
 The use of a carrier, such as crackers for
jam and frankfurters for catsup, adds to the
cost and effort, and it is often difficult to
select an appropriate carrier. Carriers are
also a source of experimental error because
the proportion of product to carrier may
not be constant, or the carrier may not be
of consistent quality.
SERVING TEMPERATURE
 The temperature at which samples are served sometimes
presents problems. For acceptance/preference tests, the
samples should be served at the temperature at which
they are normal eaten.
 Hot foods are usually served at 60oC-66oC. Ice cream
should be served at -10 to 20C and other foods at 40-
100C. To compare results, the same temperature should
be used during all tests with the product.
– Warming ovens with controlled temperature and
humidity are available.
– electrically heated beakers to keep samples hot.
– a water bath is fitted to hold beakers, and each
panelists is served from the beakers as he arrives.
– Blocks of styrofoam can be cut out to hold containers
and help maintain the temperature.
UTENSILS
 Serving utensils should not impart anv taste or
odor to the product.
 Identical containers should be used for each
sample so that no bias will be introduced from
this source.
 It is wise to use colorlessor white containers.
 Disposable dishes made from plastic, paper or
styrofoam are convenient when large numbers
are to be served, as in consumer tests, but it must
be determined beforehand that no taste is
transferres to the product.
QUANTITY OF SAMPLE
 The Sensory EvaIuation Committee ofASTM
(1968) recommends that in discrimination tests,
each panel should receive at least 16ml (0.5 oz)
of a liquid and 28g (1oz) of a solid, and the
amount should be doubled for preference test.
 The amount of sample presented should be
constant throughout the testing.
 Panelists should receive enough sample to taste
back and forth until they can make a decision.
NUMBER OF SAMPLES
 The number of samples that can be effectively
evaluated in one session should be determined
during preliminary testing.
 The type of product being tested and the
experience of the judges must be consjdered
when deciding on the number of_sarnples to test
in one sessjon.
 Motivation is an important factor in this regard.
Panelist often lose their desire to discriminate
before they lose their capability.
CODING AND ORDER OF PRESENTATION
 the order of presentation of the samples to each
panelist is randomized or balanced. In larger
experiments, the order can be obtained from
tables of random numbers.
 The code assigned to the samples should not give
the panelists any hint of the identity of the
treatments and the code itself should not
introduce any bias. Three-digit random numbers
obtained from tables of random numbers are
recommended for coding the samples.
RINSING
 The panelists are provided with an agent for oral
rinsing between samples. Taste-neutral water at
room temperature is preferred by many
investigators.
INFORMATION ABOUT SAMPLES
 As little information as possible about the test should be
given to the panelists, because this information may
influence results.
 When a panel was told that tomato juice was made from
high quality raw ingredients, the ratings were much
higher than when the same samples were presented with
the information that low quality raw products had been
used (Pettit 1958).
 This preconceived impression is called expectation error.
Panelists usually find what they expect to find. Because
of this expectation error, persons who are directly
involved with the experiment should not be included on
the panel.
CHOOSING AND TRAINING PANELISTS
Who are the panelists?
 Panelists are usually office, plant, or research
staff. It should be considered a part of work
routine for personnel to serve as panelists. Full
cooperation of the supervisors of persons who
serve as panelists is necessary. No person should
be required to evaluate the foods to which he
objects
Number of panelist
 The greater the number of persons on a semi
trained panel, the more likely it is that individual
variations will balance out.
 However, a small highly trained panel will give
more reliable results than a large untrained panel.
 A laboratory panel is usually composed of 10 to
20 persons with three or four replications by each
judge by each judge for each treatment.
Panelist condition
 Persons who serve as panelists should be in
good health and should absent themselves when
suffering from conditions that might interfere
with normal functions of taste and smell.
 Emotional factors, interest and motivation appear
to be more important than the age or sex of a
panelists.
 It is generally recommended that panelists refrain
from smoking,chewing gum, eating or drinking
for at least 30 min before testing.
 The motivation of the panelist affects his
response. An interested panelist is always more
efficient. The panelists should be made to feel
that panels are an important activity and that their
contribution is important.
Health, interest, availability, punctual, good verbal
and communication skills.
FACTORS INFLUENCING
SENSORY MEASUREMENTS
1) Expectation error
 Anv information the panelists receive about the test will
influence the results.
 Panelists usually find what they expect to find.
Therefore,panelists should not be given detailed information
about the test.
Trick
 provide only enough information for panelist to be
able to do the test
 Try not to include people already involved in the
experiment (single blind)
 Avoid codes that create inherent bias (1,A etc)
 Since people generally associate “1” or “A” with “best”, it is
recommended that three digit random numbers be used.
2) Leniency error –rate products based upon
feelings about researcher
3) Suggestion effect –response of other
panelists to product (need to isolate
panelists and keep them quiet)
4) Stimulus Error
• Influence of irrelevant questions (e.g piece
size, color, uniformity)
• Try to mask unwanted difference (e.g. colored
lights)
5) Logical error –associated with stimulus error
tendency to rate characteristics that appear to be
logically associated (yellow and rancidity).
Control by masking differences
6) Halo effect
 caused by evaluating too many factors at one
time. Panelists already have an impression
about the product when asked about second
trait –will form a logical association (e.g. dry-
> tough)
 Best to structure testing so that only one
factor is tested at a time (difficult to do)
7) Positional effect –tendency to rate second
product higher or lower
 2 products very different –panelists will
exaggerate differences and rate
‘weaker’sample lower than would
otherwise
 Use random order. Use all possible
presentation orders
DESIGNING EXPERIMENTS AND CHOOSING
METHODS OF ANALYZING DATA
Because of the variability in results of sensory
tests, interpretation cannot be made by direct
examination. The results must be tested by
statistical methods. Results are usually expressed
in degrees of significance which is the probability
that the results are caused by chance
1 2 3 4
State the basic taste on the different parts of the
tongue

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chapter3-220824082905-83d6755f.pdf

  • 1. Chapter 3 BASIC REQUIREMENTS FOR SENSORY EVALUATION
  • 2. Objectives:  At the end of this chapter, you will be able to:  Identify and determine the taste panel area.  Determine and understand the basic requirements during sensory lab.  Determine the factors to choose and train panelist.  Determine the factors influencing sensory measurements.
  • 3. Sensory laboratory  Pleasing natural shades and maintenance of comfortable temperature and humidity conditions of the laboratory set up are desirable.  Test room should be separated by suitable distance from congested are because & noise and the opportunity this would provide for unwanted socializing. Briefing room Panel room Preparation room
  • 4. Sensory Laboratory Set Up and Equipment  The physical setting must be designed so as to minimize the subject's biases, maximize their sensitizing, and eliminate variable which do not came from the product themselves.  The test area should be centrally located, easy to reach and free of crowding and confusion, as well as comfortable, quite, temperature controlled and free from odour & noise.
  • 6. 1. The testing booths are located should be separated from sample preparation room and wash room and store by a complete partition. 2. A temperature of about 20°C and RH of 62% in the testing room in considered to be ideal. 3. The booths may be fairly simple or very elaborate, depending on the funds available. 4. Many laboratories have a sink and tap built into each booth for expectoration and to provide water for rinsing. 5. For descriptive analvsis and panel training sessions a round table that permits discussion is required. 6. The color of the booths should not influence the appearance of the product being judged. An off-white or light gray color is usually recommended.
  • 7. LIGHTING  Lighting should be uniform and should not influence the appearance of the product to be tested. The type of light used should be carefully chosen if color and appearance are important factors to be judged, since many fluorescent lights distort color.  A common feature of many panel booths is a choice of red, green, and / or blue lighting at low intensity obtained through the use of colored bulbs or special filters. The lights are used to mask visual differences between samples in differences tests calling for the subject to determine by taste ( or by feel, if appropriate) which samples are identical.
  • 8. TESTING SCHEDULE  The time of day that tests are run influences results. Although this cannot be controlled if the number of tests is large, late morning and mid afternoon are generally the best times for testing.
  • 9. The type of preparation equipment located in the sample preparation area depends on the products being tested.A well-equipped kitchen is a good start, with specialized equipment being added according to need.  The preparation area should have a good ventilation system for removal of cooking odors. Sufficient counter space for serving and assembling samples for presentation is an important requirement (Fig. 3). PREPARATION ROOM & SAMPLES PREPARATION
  • 10. • A laboratory bench flush with the hatches so that samples trays will side through. The hatch on the service counter should preferably be constructed in such a manner that there shall be no recognition of individual or either side of partition. • Benches, kitchen range, ovens, etc. for preparation. • Refrigerator and freezer for storage of samples. • Storage for glassware, dishes, glasses, trays, etc. • Dishwashers, disposers, trash compactors, wastebaskets, sinks, etc. • Frozen storage for panel member treats, if used • Large garbage containers for quick disposal of used product, etc.
  • 11.
  • 12. Preliminary testing  Preliminary testing is usually necessary to determine the method of preparation of the product.  All the various factors, such as time and temperature of thawing and cooking, amount of water and size of pot for boiling, and time and speed of blendjng, should be predetermined and kept constant throughout tests on the product.  The_preparation method should not impart any foreign tastes or odors to the product.
  • 13.  Panelists are influenced by irrelevant characteristics of the samples. Because of this, every effort should be made to make the samples from different treatments identical in all characteristics except the one being judged. It is sometimes necessary to grind dice, chop, or puree the samples to obtain uniformity.  However, Kefford and Christie (1960) found that judges prefer foods in their normal state. When conducting acceptance or preference tests, it may be better to present one sample at a time rather than grind or puree the product to mask the differences.
  • 14. DILUTION AND CARRIERS  Most foods are served in the way in which they are normally consumed.  The use of a carrier, such as crackers for jam and frankfurters for catsup, adds to the cost and effort, and it is often difficult to select an appropriate carrier. Carriers are also a source of experimental error because the proportion of product to carrier may not be constant, or the carrier may not be of consistent quality.
  • 15. SERVING TEMPERATURE  The temperature at which samples are served sometimes presents problems. For acceptance/preference tests, the samples should be served at the temperature at which they are normal eaten.  Hot foods are usually served at 60oC-66oC. Ice cream should be served at -10 to 20C and other foods at 40- 100C. To compare results, the same temperature should be used during all tests with the product. – Warming ovens with controlled temperature and humidity are available. – electrically heated beakers to keep samples hot. – a water bath is fitted to hold beakers, and each panelists is served from the beakers as he arrives. – Blocks of styrofoam can be cut out to hold containers and help maintain the temperature.
  • 16. UTENSILS  Serving utensils should not impart anv taste or odor to the product.  Identical containers should be used for each sample so that no bias will be introduced from this source.  It is wise to use colorlessor white containers.  Disposable dishes made from plastic, paper or styrofoam are convenient when large numbers are to be served, as in consumer tests, but it must be determined beforehand that no taste is transferres to the product.
  • 17. QUANTITY OF SAMPLE  The Sensory EvaIuation Committee ofASTM (1968) recommends that in discrimination tests, each panel should receive at least 16ml (0.5 oz) of a liquid and 28g (1oz) of a solid, and the amount should be doubled for preference test.  The amount of sample presented should be constant throughout the testing.  Panelists should receive enough sample to taste back and forth until they can make a decision.
  • 18. NUMBER OF SAMPLES  The number of samples that can be effectively evaluated in one session should be determined during preliminary testing.  The type of product being tested and the experience of the judges must be consjdered when deciding on the number of_sarnples to test in one sessjon.  Motivation is an important factor in this regard. Panelist often lose their desire to discriminate before they lose their capability.
  • 19. CODING AND ORDER OF PRESENTATION  the order of presentation of the samples to each panelist is randomized or balanced. In larger experiments, the order can be obtained from tables of random numbers.  The code assigned to the samples should not give the panelists any hint of the identity of the treatments and the code itself should not introduce any bias. Three-digit random numbers obtained from tables of random numbers are recommended for coding the samples.
  • 20. RINSING  The panelists are provided with an agent for oral rinsing between samples. Taste-neutral water at room temperature is preferred by many investigators.
  • 21. INFORMATION ABOUT SAMPLES  As little information as possible about the test should be given to the panelists, because this information may influence results.  When a panel was told that tomato juice was made from high quality raw ingredients, the ratings were much higher than when the same samples were presented with the information that low quality raw products had been used (Pettit 1958).  This preconceived impression is called expectation error. Panelists usually find what they expect to find. Because of this expectation error, persons who are directly involved with the experiment should not be included on the panel.
  • 22. CHOOSING AND TRAINING PANELISTS Who are the panelists?  Panelists are usually office, plant, or research staff. It should be considered a part of work routine for personnel to serve as panelists. Full cooperation of the supervisors of persons who serve as panelists is necessary. No person should be required to evaluate the foods to which he objects
  • 23. Number of panelist  The greater the number of persons on a semi trained panel, the more likely it is that individual variations will balance out.  However, a small highly trained panel will give more reliable results than a large untrained panel.  A laboratory panel is usually composed of 10 to 20 persons with three or four replications by each judge by each judge for each treatment.
  • 24. Panelist condition  Persons who serve as panelists should be in good health and should absent themselves when suffering from conditions that might interfere with normal functions of taste and smell.  Emotional factors, interest and motivation appear to be more important than the age or sex of a panelists.  It is generally recommended that panelists refrain from smoking,chewing gum, eating or drinking for at least 30 min before testing.
  • 25.  The motivation of the panelist affects his response. An interested panelist is always more efficient. The panelists should be made to feel that panels are an important activity and that their contribution is important. Health, interest, availability, punctual, good verbal and communication skills.
  • 27. 1) Expectation error  Anv information the panelists receive about the test will influence the results.  Panelists usually find what they expect to find. Therefore,panelists should not be given detailed information about the test. Trick  provide only enough information for panelist to be able to do the test  Try not to include people already involved in the experiment (single blind)  Avoid codes that create inherent bias (1,A etc)  Since people generally associate “1” or “A” with “best”, it is recommended that three digit random numbers be used.
  • 28. 2) Leniency error –rate products based upon feelings about researcher 3) Suggestion effect –response of other panelists to product (need to isolate panelists and keep them quiet)
  • 29. 4) Stimulus Error • Influence of irrelevant questions (e.g piece size, color, uniformity) • Try to mask unwanted difference (e.g. colored lights) 5) Logical error –associated with stimulus error tendency to rate characteristics that appear to be logically associated (yellow and rancidity). Control by masking differences
  • 30. 6) Halo effect  caused by evaluating too many factors at one time. Panelists already have an impression about the product when asked about second trait –will form a logical association (e.g. dry- > tough)  Best to structure testing so that only one factor is tested at a time (difficult to do)
  • 31. 7) Positional effect –tendency to rate second product higher or lower  2 products very different –panelists will exaggerate differences and rate ‘weaker’sample lower than would otherwise  Use random order. Use all possible presentation orders
  • 32. DESIGNING EXPERIMENTS AND CHOOSING METHODS OF ANALYZING DATA Because of the variability in results of sensory tests, interpretation cannot be made by direct examination. The results must be tested by statistical methods. Results are usually expressed in degrees of significance which is the probability that the results are caused by chance
  • 33. 1 2 3 4 State the basic taste on the different parts of the tongue