characterization of cell line

1. CELL LINE
CHARACTERISATION
Presentation by – Teena
Roll no – 5
Submitted to –kurukshetra department
DR. Sulekha Chahal .

2. Need for characterization
• Demonstration of the absence of cross
contamination
• Confirmation of species of origin
• Determination of whether the cell line is
transformed or not.
• Indication to whether cell line is prone to
genetic instability and phenotypic variations.
• Identification of specific cell line within a
group from the same origin.

3. AUTHENTICATION
• For checking the cross contamination it is
necessary to check the authenticity of cell line
It can be done in various ways. Some of the
methods in general use for cell line
characterization are

4. Criteria Method
Karyotype Chromosome spread with
banding
Isoenzyme analysis Agar gel electrophoresis
Cell surface antigen Immunohistochemistry
Cytoskeleton Immunocytochemistry with
antibodies to specific
cytokeratins
DNA Fingerprint Restriction enzyme digest;
PAGE ; Satellite DNA probes

5. Cell Morphology
•Simplest and most directed
technique use to identify cells
•Shortcomings- cellular morphology
varies in response to culture
conditions
•Comparative observation should
always be made at some stage of
growth and cell density in same
medium and on same substrate.

6. Cell morphology study
1)Microscopy
It could be use to study the
morphology and anatomy of cells
2)Photomicroscopy
ie photograph plus microscopy
3)Staining

7. Chromosome content
•For identifying cell line and relating them species
and sex from which they have derived
•Also diffrentiate normal and transformed cells
•Includes
1. Chromosome banding(casperson etal in 1970)
• C banding-stain centromere
• Q banding- stain AT rich region
• G banding-stain sulphur rich protein part
R banding-stain sulphur deficient region
2. Chromosome analysis
3.Chromosome painting