1. Life of the Mind 2011 Facilitator Training Presenter: Cynthia Peterson
2. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
3. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
4. What is the science of Cell Biology? Work with cells in laboratory setting Learn about cell structure, division, organization, normal and disease processes Microscopy gives an up-close picture of the cells Important step in drug development
5. What is the science of Cell Biology? Work with cells in laboratory setting Learn about cell structure, division, organization, normal and disease processes Microscopy gives an up-close picture of the cells Important step in drug development
7. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
8. What was the breakthrough with HeLa Cells? Human cells cultured for the first time! (Gey had cultured other species and had worked for over 20 years with this aim) Cancer researchers considered cultured human cells to hold the key to discovering a cure HeLa cells are a “workhorse” for cell biology: easy to grow, ideal for microscopy, transfection Provided a model cell line for studying normal cellular processes as well as many diseases
10. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
11. What milestones in cell biology were possible because of HeLa cells? Polio infection process and vaccine development Advances in microscopy Research to isolate single cells and eventually to establish clonal cell lines Methods established for chromosome spreading and karyotyping Ongoing research on telomerase (Nobel Prize 2009) New siRNA technologies for gene knockdowns
13. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
14. How does one scientist react to this book? Medicine and Science History of modern medicine Doctor/Patient Relationship Ethics, Values, and Civility Research Ethics Legal Issues
15. How does one scientist react to this book? Research Journalism Finding a passion Social, Economic and Political Aspects Socioeconomic status Race, class and gender
16. Questions to consider… What is the science of Cell Biology? What was the breakthrough with HeLa cells? What milestones in cell biology studies were possible because of HeLa cells? How does one scientist react to this book? What about research at the University of Tennessee?
17. What about research at the University of Tennessee? HeLa cells are used by many researchers across campus….. Where is a protein in the cell? What are its binding partners? How does localization change with cell cycle? Use transfection methods to introduce new genes into the cell Use knockdown technologies to selectively reduce levels of specific proteins Examples from my own department Mobility of proteins in membrane Localize proteins within nuclear envelope Finding specific DNA/protein interactions An example from an undergraduate researcher
