This document discusses the application of next-generation sequencing (NGS) technologies to preimplantation genetic diagnosis (PGD). It provides a brief history of PGD and explains the use of NGS-based PGD to detect chromosomal abnormalities in embryos before implantation. The document outlines the PGD workflow using NGS, which involves sequencing embryos and analyzing the data using hidden Markov models and other statistical methods to determine ploidy status and identify chromosomal abnormalities. Benefits of NGS-based PGD include lower costs relative to array-based methods and the ability to analyze multiple embryos in a single run.