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Weiss lab
Internship fall 2014
Snowing
Mentor
Franzi Uhl
• PhD in natural science
• Masters in bioengineering
• From Germany
• Thanks for being an awesome
mentor
What I did
 Projects
 Human and Pig lung decel
photo protocol
 Science fair: preventing
contamination in decellularized
mouse lungs
Responsibilities
 decelling mouse lungs
 Removing mouse lungs
from mice
 Helping with human and
pig lung decell
 Mixing solutions
 Desk work
Mixing solutions
Measuring liquids, using scale, autoclaving, sterile filtering etc.
Preventing
contamination in
decellurized mouse
lungs
Science fair project 2014 - 15
• WHY
• Many people need lung transplants
• decellularizing is the first step to
growing lungs
• Experiments are done on mouse lungs
• decellularized mouse lungs often get
contaminated with bacteria
Science fair project
• WHAT:
• Researching pen-strep
antibiotics, agar, etc
• growing bacteria from samples
taken each day of decell, to find
out when contamination occurs
• Doubling the quantity of
penicillin-streptomycin to
prevent contamination
• RESULTS:
• Coming next term
Pig lung decellularization photo protocol
How to make agar plates
 Teaching a skill
Mix agar and DI water
Mix 23 grams agar per
Liter of water
Microwave In 1 minute
intervals until agar has
dissolved(about 2 minutes)
Mixing agar
Autoclave agar to insure
sterility
Allow to cool
Label all solutions with
content initials and date
Teaching a skill
Pouring the plates
Make sure agar is a
liquid. If not
microwave to
liquify
Tip up lid of plate
and pore agar slowly
to avoid bubbles Seal plate with
parafilm, store upside-
down in fridge
What I learned
 Decellularizing pig and human lungs
 Using google drawing program
 Making agar
 Writing protocol for science fair

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Weiss lab

  • 3. Mentor Franzi Uhl • PhD in natural science • Masters in bioengineering • From Germany • Thanks for being an awesome mentor
  • 4. What I did  Projects  Human and Pig lung decel photo protocol  Science fair: preventing contamination in decellularized mouse lungs Responsibilities  decelling mouse lungs  Removing mouse lungs from mice  Helping with human and pig lung decell  Mixing solutions  Desk work
  • 5. Mixing solutions Measuring liquids, using scale, autoclaving, sterile filtering etc.
  • 6. Preventing contamination in decellurized mouse lungs Science fair project 2014 - 15 • WHY • Many people need lung transplants • decellularizing is the first step to growing lungs • Experiments are done on mouse lungs • decellularized mouse lungs often get contaminated with bacteria
  • 7. Science fair project • WHAT: • Researching pen-strep antibiotics, agar, etc • growing bacteria from samples taken each day of decell, to find out when contamination occurs • Doubling the quantity of penicillin-streptomycin to prevent contamination • RESULTS: • Coming next term
  • 8. Pig lung decellularization photo protocol
  • 9.
  • 10.
  • 11.
  • 12. How to make agar plates  Teaching a skill Mix agar and DI water Mix 23 grams agar per Liter of water Microwave In 1 minute intervals until agar has dissolved(about 2 minutes) Mixing agar Autoclave agar to insure sterility Allow to cool Label all solutions with content initials and date
  • 13. Teaching a skill Pouring the plates Make sure agar is a liquid. If not microwave to liquify Tip up lid of plate and pore agar slowly to avoid bubbles Seal plate with parafilm, store upside- down in fridge
  • 14. What I learned  Decellularizing pig and human lungs  Using google drawing program  Making agar  Writing protocol for science fair