My internship

1. Weiss lab
Internship fall 2014

2. Snowing

3. Mentor
Franzi Uhl
• PhD in natural science
• Masters in bioengineering
• From Germany
• Thanks for being an awesome
mentor

4. What I did
 Projects
 Human and Pig lung decel
photo protocol
 Science fair: preventing
contamination in decellularized
mouse lungs
Responsibilities
 decelling mouse lungs
 Removing mouse lungs
from mice
 Helping with human and
pig lung decell
 Mixing solutions
 Desk work

5. Mixing solutions
Measuring liquids, using scale, autoclaving, sterile filtering etc.

6. Preventing
contamination in
decellurized mouse
lungs
Science fair project 2014 - 15
• WHY
• Many people need lung transplants
• decellularizing is the first step to
growing lungs
• Experiments are done on mouse lungs
• decellularized mouse lungs often get
contaminated with bacteria

7. Science fair project
• WHAT:
• Researching pen-strep
antibiotics, agar, etc
• growing bacteria from samples
taken each day of decell, to find
out when contamination occurs
• Doubling the quantity of
penicillin-streptomycin to
prevent contamination
• RESULTS:
• Coming next term

8. Pig lung decellularization photo protocol

12. How to make agar plates
 Teaching a skill
Mix agar and DI water
Mix 23 grams agar per
Liter of water
Microwave In 1 minute
intervals until agar has
dissolved(about 2 minutes)
Mixing agar
Autoclave agar to insure
sterility
Allow to cool
Label all solutions with
content initials and date

13. Teaching a skill
Pouring the plates
Make sure agar is a
liquid. If not
microwave to
liquify
Tip up lid of plate
and pore agar slowly
to avoid bubbles Seal plate with
parafilm, store upside-
down in fridge

14. What I learned
 Decellularizing pig and human lungs
 Using google drawing program
 Making agar
 Writing protocol for science fair