1) The document describes the construction of a plasmid to produce the monoclonal antibody Trastuzumab (Herceptin) in Pichia pastoris yeast for treatment of HER2-positive breast cancer. Genes for the heavy and light chains of Trastuzumab were optimized for expression in P. pastoris and inserted into an expression vector. 2) The vector was used to transform P. pastoris cells, which were then cultured and induced to express and secrete Trastuzumab into the culture medium. 3) The expressed Trastuzumab was characterized, affinity purified using a protein A column, and formulated into lyophilized vials for therapeutic use, with each vial containing 150 mg