Tomato Genome Build: SL2.5 to SL3.0
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The document summarizes updates to the tomato genome sequence including the release of builds SL2.50 and SL3.0. SL3.0 integrated over 1000 BAC sequences into SL2.50, improving contiguity and reducing gaps. Future work includes integrating sequences from chromosome 0, validating the assembly with optical maps, improving gene annotations, and continuing to refine the genome sequence.
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Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Pests of Storage_Identification_Dr.UPR.pdf
InIndia-post-harvestlosses-unscientificstorage,insects,rodents,micro-organismsetc.,accountforabout10percentoftotalfoodgrains
Graininfestation
Directdamage
Indirectly
•theexuviae,skin,deadinsects
•theirexcretawhichmakefoodunfitforhumanconsumption
About600speciesofinsectshavebeenassociatedwithstoredgrainproducts
100speciesofinsectpestsofstoredproductscauseeconomiclosses
Authoring a personal GPT for your research and practice: How we created the Q...
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
快速办理(UAM毕业证书)马德里自治大学毕业证学位证一模一样
学校原件一模一样【微信：741003700 】《(UAM毕业证书)马德里自治大学毕业证学位证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
Bob Reedy - Nitrate in Texas Groundwater.pdf
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meeting
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
Ozturkcan, S., Berndt, A., & Angelakis, A. (2024). Mending clothing to support sustainable fashion. Presented at the 31st Annual Conference by the Consortium for International Marketing Research (CIMaR), 10-13 Jun 2024, University of Gävle, Sweden.
Micronuclei test.M.sc.zoology.fisheries.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
8.Isolation of pure cultures and preservation of cultures.pdf
Isolation of pure culture, its various method.
The cost of acquiring information by natural selection
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Shallowest Oil Discovery of Turkiye.pptx
The Petroleum System of the Çukurova Field - the Shallowest Oil Discovery of Türkiye, Adana
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Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Authoring a personal GPT for your research and practice: How we created the Q...
Authoring a personal GPT for your research and practice: How we created the Q...
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
20240520 Planning a Circuit Simulator in JavaScript.pptx
20240520 Planning a Circuit Simulator in JavaScript.pptx
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
8.Isolation of pure cultures and preservation of cultures.pdf
The cost of acquiring information by natural selection
The cost of acquiring information by natural selection
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
Tomato Genome Build: SL2.5 to SL3.0
- 1. Tomato Genome Build SL2.5 SL3.0 Surya Saha, Jeremy Edwards, Prashant Hosmani, Mirella Flores and Lukas Mueller Sol Genomics Network (SGN) Boyce Thompson Institute, Ithaca, NY ss2489@cornell.edu @SahaSurya Slides: http://bit.ly/SOL15bld3
- 2. Tomato Genome SGN Workshop, SOL 2015 Whole-genome shotgun and full-length BAC sequences • 454 reads (filtered, 22X) • BAC end Sanger reads (0.18X) • Fosmid end Sanger reads (0.087X) • Selected BACs • Sanger reads (5.2X).
- 3. Tomato Build SL2.40 SL2.50 SGN Workshop, SOL 2015 Lindsay Shearer Stephen Stack
- 4. SL2.50 Availability JBrowse • Locus pages • Gene pages • FTP site Also at NCBI http://www.ncbi.nlm.nih.gov/assembly/GCF_000188115.3/ SGN Workshop, SOL 2015
- 5. CHROMOSOMES SCAFFOLDSCONTIGS Gene to Genome – The BIG picture CONTIG GAPS SCAFFOLD GAPS SGN Workshop, SOL 2015 GENES MAP (chr1) Ovate (chr1) TM2 (chr 9) L2 (chr 10)
- 6. State of the SL2.50 Build SGN Workshop, SOL 2015 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% 0 1 2 3 4 5 6 7 8 9 10 11 12 Sequence Scaffold gap length Component gap length
- 7. State of the SL2.50 Build SGN Workshop, SOL 2015 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% 0 1 2 3 4 5 6 7 8 9 10 11 12 Sequence Scaffold gap length Component gap length Length 823Mb Sequence 737Mb Contig gaps 43Mb (5.30%) Scaffold gaps 42Mb (5.17%) Total gaps 86Mb (10.47%)
- 8. Tomato BAC Resources Bruce Roe HTGS Phase 1: 332 HTGS Phase 2: 520 HTGS Phase 3: 2764 http://www.ncbi.nlm.nih.gov/genbank/htgs/faq SGN Workshop, SOL 2015 ftp://ftp.solgenomics.net/tomato_genome/bacs/
- 9. SGN Workshop, SOL 2015 BAC libraries • HindIII (129,024 clones) • EcoRI (72,264 clones) • MboI (52,992 clones) Tomato BAC Resources… 2764 Phase 3 BACs 340,000 high-quality reads 20X of entire genome Mueller et al. 2009
- 10. SGN Workshop, SOL 2015 ITAG 2.4 Genes SL2.50 Genome Whole Genome Shotgun assembly Phase 3 BACs 20kb or more
- 11. SGN Workshop, SOL 2015 ITAG 2.4 Genes SL2.50 Genome Whole Genome Shotgun assembly Phase 3 BACs 20kb or more No BACs mapped here!!
- 12. Workflow SGN Workshop, SOL 2015 Automatic integration of BACs Manual validation NCBI validation
- 13. Workflow SGN Workshop, SOL 2015 Automatic integration of BACs Manual validation NCBI validation https://github.com/solgenomics/Bio-GenomeUpdate BAC assemblies Align to SL2.50 • 500bp BAC ends • 100% identity Jeremy Edwards Place BACs
- 14. Phrap Assembly (HTGS Phase 3 BACs) SGN Workshop, SOL 2015 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% 1 2 3 4 5 6 7 8 9 10 11 12 Assembled BACs Singleton BACs
- 15. Phrap Assembly (HTGS Phase 3 BACs) SGN Workshop, SOL 2015 Chr10 Contig68 10 BACs (242Kb!!) Chr2 Contig185 7 BACs (566Kb!!)
- 16. Workflow SGN Workshop, SOL 2015 Automatic integration of BACs Manual validation NCBI validation Prashant Hosmani Mirella Flores
- 17. Workflow SGN Workshop, SOL 2015 Automatic integration of BACs Manual validation NCBI validation
- 18. SGN Workshop, SOL 2015 0 50 100 150 200 250 300 350 400 450 1 2 3 4 5 6 7 8 9 10 11 12 1041/2764 BACs Integrated • No contained BACs • No single-end mapping BACs
- 19. SGN Workshop, SOL 2015 Improved Contiguity 0 500 1000 1500 2000 2500 3000 1 2 3 4 5 6 7 8 9 10 11 12 SL2.50 components SL3.0 components
- 20. Gap Reduction 0 500000 1000000 1500000 2000000 2500000 3000000 3500000 4000000 4500000 1 2 3 4 5 6 7 8 9 10 11 12 SL2.50 Ns SL3.0 Ns
- 21. Future BioNano optical maps • Integrate sequences from chr 0 into chrs 1-12 • Validation • Improved gap sizing SGN Workshop, SOL 2015 Gabino Sanchez
- 22. Future… Annotation of SL3.0 • Lift over ITAG 2.4 annotations from SL2.50 • Maker annotations • ITAG 3.0 annotations SGN Workshop, SOL 2015
- 23. Thank you!! Questions?? SGN Workshop, SOL 2015 Slides: http://bit.ly/SOL15bld3