1) Several studies have evaluated discontinuing tyrosine kinase inhibitor (TKI) treatment in chronic myeloid leukemia patients who have achieved a deep molecular response.
2) These studies found that 40-60% of patients were able to remain in treatment-free remission for varying periods of time after stopping TKI treatment.
3) Sustaining a complete molecular response for at least 2 years before discontinuing treatment appears important for treatment-free remission success rates. Close molecular monitoring after stopping treatment is also needed.
Dr. David Vesole, Co-Chief, Multiple Myeloma at John Theurer Cancer Center at HackensackUMC presentation at the MMRF Clinical Insights program in April 2012.
Dr. David Vesole, Co-Chief, Multiple Myeloma at John Theurer Cancer Center at HackensackUMC presentation at the MMRF Clinical Insights program in April 2012.
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric...Dr Siddartha
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Basavatarakam Indo-American Cancer Hospital and Research Institute
An array of presentation of lymphoma spillover in the peripheral smear and bone marrow. All types of lymphomas are discussed along with a bouquet of HPE pictures
The angiogenesis process, the factors regulating it, different assays for it, a little about tumour angiogenesis, the drugs and new therapeutic approaches towards inhibiting or augmenting the process.
WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues : 2016 U...Ankit Raiyani
This is a complilation of expected changes in the myeloid neoplasms in the upcoming 2016 update of the "WHO classification of tumours of haematopoietic and lymphoid tissues".
Some of the changes may not be incorporated in the actual published book.
This compilation has been prepared from presentations from persons actually concerned with revision of the book. All credits goes to them.
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric...Dr Siddartha
Lab Diagnosis of Chronic lymphoproliferative disorders (CLPD);Flowcytometric Evaluation
Basavatarakam Indo-American Cancer Hospital and Research Institute
An array of presentation of lymphoma spillover in the peripheral smear and bone marrow. All types of lymphomas are discussed along with a bouquet of HPE pictures
The angiogenesis process, the factors regulating it, different assays for it, a little about tumour angiogenesis, the drugs and new therapeutic approaches towards inhibiting or augmenting the process.
WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues : 2016 U...Ankit Raiyani
This is a complilation of expected changes in the myeloid neoplasms in the upcoming 2016 update of the "WHO classification of tumours of haematopoietic and lymphoid tissues".
Some of the changes may not be incorporated in the actual published book.
This compilation has been prepared from presentations from persons actually concerned with revision of the book. All credits goes to them.
Mutations in Chronic myeloid leukaemia and Imatinib resistanceDr Sandeep Kumar
some corrections over previous presentation on CML. Covers topics like - pathophysiology of CML, Mutations discussed in detail, TKI resistance in various mutations and treatment options. Also Imatinib resistance has been discussed in detail.
Chronic myelogenous leukemia (CML) - pluripotential stem cell disease
A malignancy the treatment of which has been revolutionised over the last decade.
Here is a comprehensive discussion on the disease
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Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
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Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
1. TKI Discontinuation in CML
Udomsak Bunworasate
King Chulalongkorn Memorial Hospital
Chulalongkorn University
2. BCR/ABL Tyrosine Kinase Inhibitors (TKI)
Imatinib
Glivec, STI-571 Dasatinib
Sprycel, BMS-354825
Nilotinib
Tasigna, AMN-107
• Survival of CML pts. who respond to treatment is close to
the healthy population.
• TKI discontinuation in responded pts. has been proposed
due to
- Economic impact of treating patients during lifetime
- Quality of life, long-term tolerability/toxicity (esp. in younger pts.)
4. To stop imatinib, it is necessary to achieve and
sustain very low level of residual disease
Michor F et al. Nature 2005, 435:1267-70 Mahon et al. ASH 2013 [abstract 255]
5.
6. Study Rx before Response for Definition of relapse TFR %
discontinuation discontinuation (median f/U)
STIM1 IFN->I* for MR4.5 for ≥2 yrs Loss ofMMR or ≥1-log 40 % (55mos)
≥3 yrs or increase in BCR-ABL
STIM2 I for ≥3 yrs MR4.5 for ≥2 yrs Loss ofMMR or ≥1-log 46 %at 2 yrs
or increase in BCR-ABL
ALLG CML8 I for ≥3 yrs MR4.5 for ≥2 yrs Loss of MMR or confirmed 42.7 % (42 mos)
(TWISTER) loss of MR4.5
A-STIM I for ≥3 yrs MR4.5 for ≥2 yrs Loss of MMR 64 % (23 mos)
EUROSKI I, N*, D* MR4 for ≥1 yr Loss of MMR 61 % at 6 mos.
(in progress)
STOP 2G-TKI N, D CMR for median Loss of MMR 61.1 %
29 mos (preliminary)
Discontinuation Studies in CML-CP
I=Imatinib, N=Nilotinib, D=Dasatinib
Mahon et al. Ann Hematol 2015,94:S187-93
7. TWISTER
FREQUENT AND SUSTAINED DRUG-FREE
REMISSION IN THE AUSTRALASIAN CML8 TRIAL OF
IMATINIB WITHDRAWAL
Median follow-up of 42 months (range 15 – 72)
Ross et al. Blood 2013;122(4):515-22
8. Sustained CMR for at least 2 yrs is of
major importance in TKI discontinuation
78%
15%
p = 0.0002
Takahashi et al. Haematologica 2012; 97(6): 903-6
9. Study Rx before Response for Definition of relapse TFR %
discontinuation discontinuation (median f/U)
STIM1 IFN->I* for MR4.5 for ≥2 yrs Loss ofMMR or ≥1-log 40 % (55mos)
≥3 yrs or increase in BCR-ABL
STIM2 I for ≥3 yrs MR4.5 for ≥2 yrs Loss ofMMR or ≥1-log 46 %at 2 yrs
or increase in BCR-ABL
ALLG CML8 I for ≥3 yrs MR4.5 for ≥2 yrs Loss of MMR or confirmed 42.7% (42 mos)
(TWISTER) loss of MR4.5
A-STIM I for ≥3 yrs MR4.5 for ≥2 yrs Loss of MMR 64 % (23 mos)
EUROSKI I, N*, D* MR4 for ≥1 yr Loss of MMR 61 % at 6 mos.
(in progress)
STOP 2G-TKI N, D CMR for median Loss of MMR 61.1 %
29 mos (preliminary)
Discontinuation Studies in CML-CP
I=Imatinib, N=Nilotinib, D=Dasatinib
Mahon et al. Ann Hematol 2015,94:S187-93
10. “According to STIM” (A-STIM)
Loss of MMR As a Trigger for Restarting Imatinib
• Enrolled patients with MR4.5 or UMRD for ≥ 2 years on imatinib
• After discontinuation, loss of MMR was used as the criteria for
restarting imatinib
• All patients who re-initiated imatinib therapy after a loss of MMR
regained MR4.5 after a median of 7.3 months of treatment
Relapse-free Survival by Relapse Criteria
50
25
0
PercentRelapse-free(%)
0 6 12 18 24 30 36 42 48
Months
54 60 66 72 78 84 90 96
100
75
P < .0001
Without loss of MMR: 63.7%
STIM criteria*: 38.1%
Without loss of CMR: 26.5%
*Two consecutive increasing values of detectable
MRD
Rousselot et al. Haematologica. 2012;97(s1): 77 [abstract 194].
Rousselot et al . J Clin Oncol 2014;32:424-430
11. • In the STIM study, a fluctuation in BCR-ABL transcript was
detected in 5 of the 39 without confirmed molecular relapse.
Mahon et al. Lancet Oncol 2010; 11: 1029–35
Fluctuation of BCR-ABL detection after
discontinuation
12. BCR-ABL DNA evidence of residual disease
in patients with UMRD
Ross DM et al. Blood 2013;122(4):515-22.
13.
14. Conclusion
• Around 40% of CML pts. with stable deep
molecular response for at least 2 yrs. are likely to
remain in prolonged TFR after treatment
discontinuation
• The rate is around 60% if the loss of MMR is the
criteria of molecular recurrence
• In most cases, TKI discontinuation needs to be
done under a clinical trial with close molecular
monitoring
• A long-term follow-up of the discontinuation
studies will be necessary to affirm cure
Editor's Notes
The success of imatinib and second-generation tyrosine kinase inhibitors (TKIs) has dramatically changed the outcome for CML patients.
Bosutinib
Ponatinib
identifying patients who can safely stop TKI therapy may reduce long-term medical costs.
In recent years, almost 1,000 patients have officially stopped TKI around the world for molecular efficacy in clinical trials.
Pharma study
Discontinuation of imatinib in pts. after 1–3 yrs led to a rapid increase of leukemic cells to levels at or beyond pretreatment baseline.
We conclude that leukaemic stem cells, which drive CML disease, are not depleted by imatinib therapy. The rapid upslope of 0.09 ^ 0.05 per day corresponds to a doubling time of roughly 8 days, which characterizes the rate at which differentiated leukaemic cells are regenerated from leukemic stem cells.
a multicenter study entitled “Stop Imatinib” (STIM) trial and three cases of late relapse were observed at months 19, 20, and 22, respectively. Most patients who experienced molecular relapse did so within 6 months of imatinib cessation and remained responsive to re-treatment with imatinib.
CMR was defined by undetectable molecular response (UMR) with PCR sensitivity between 4.5 and 5 log.
Molecular relapse, which was arbitrarily defined as two positiveRQ-PCR results over a period of 1month showing a significant rise (1 log) in BCR-ABL transcripts,
was a trigger for imatinib treatment again.
STIM1: Fifty-one percent of the patients had been previously treated with IFN, and the other half were treated with imatinib only.
We still do not know the threshold of residual disease, which will allow us to safely stop TKI with the lowest rate of molecular recurrence. It is one
the aims of the EURO-SKI study.
(EURO-SKI) trial from the European LeukemiaNet (ELN) that is currently in progress in ten countries. The criteria for discontinuation are less strict than in
the STIM studies: the duration of TKI treatment before enrolment must be at least 3 years and no PCR-results >0.01 % within the last year, i.e., a sustained deep molecular response
(DMR) of 4 log have to be confirmed.
A nationwide survey in Japan identified 50 patients who had discontinued imatinib
Takahashi et al. reported that a significant difference in the estimated molecular relapse-free survival rates at 5 years following discontinuation between
patients in whomCMRwas sustained for more than 24months prior to imatinib discontinuation and those sustaining a CMR for less than 24 months (78 vs. 15 %, p=0.0002)
STIM1: Fifty-one percent of the patients had been previously treated with IFN, and the other half were treated with imatinib only.
We still do not know the threshold of residual disease, which will allow us to safely stop TKI with the lowest rate of molecular recurrence. It is one
the aims of the EURO-SKI study.
(EURO-SKI) trial from the European LeukemiaNet (ELN) that is currently in progress in ten countries. The criteria for discontinuation are less strict than in
the STIM studies: the duration of TKI treatment before enrolment must be at least 3 years and no PCR-results >0.01 % within the last year, i.e., a sustained deep molecular response
(DMR) of 4 log have to be confirmed.
A multicenter observational study (A-STIM [According to Stop Imatinib]) evaluating MMR persistence was conducted in 80 patients with CP-CML who had stopped imatinib after
prolonged CMR.
One patient diagnosed in 1996 (15 years ago) experienced lymphoid blast crisis 8.5 months after
restarting imatinib while in MMR
Fluctuation of BCR-ABL transcript levels below the MMR threshold (greater than or equal to two consecutive positive values) was observed
in 31 % of patients after imatinib discontinuation.
It should be noted that leukemic cells may still be present even if RQ-PCR results are negative.
Current RQ-PCR methods can reliably detect up to a 5-log reduction in BCR-ABL, but
newer techniques, such as DNA-based PCR, RNA-based digital PCR, and replicated PCR, have demonstrated increased
sensitivities and may enable the assessment of even deeper levels of molecular response.
However, it should be noted that using an ultrasensitive PCR technique, a low level of BCR-ABL transcripts has been found in the blood of normal
individuals, suggesting that a complete absence of transcripts may not be required to eradicate the disease.
In the TWISTER study using PCR on DNA which is a non routine technique increasing the sensitivity as compared to classical RQ-PCR (decimal
logarithm twice) to analyze patient which were considered in the so- called undetectable UMRD, leukemic cells were exhibited in all cases.
Two independent studies have been reported that low NK cell numbers may predict early disease relapse after TKI discontinuation. These studies suggest that NK cell-based
immune surveillance may contribute to CML control after TKI cessation.
That is why John Goldman proposed some years ago the definition of “operational cure”