This study evaluated seasonal changes in hydrologic connectivity in a tile-drained agricultural catchment using environmental tracers. Samples were collected from piezometers and lysimeters throughout the site and from a tile drain. The goals were to determine the contributions of precipitation, shallow soil water, and deep soil water to tile drainage over the water year. It was hypothesized that early in the year, the deep and shallow groundwater systems would be isolated due to wetting of the soil profile, but would later mix as the profile saturated. Findings showed the primary source of water to the tile drain was the deep system, and values converged in the deep, shallow, and tile drain waters later in the high-flow season, suggesting mixing.