The document discusses the role of subspecies in big cat conservation by analyzing genetic data from 134 tigers. It finds strong support for distinguishing 6 tiger subspecies based on mitochondrial DNA and microsatellite data. Phylogenetic analysis reveals distinct genetic clusters that correlate with geographic regions, supporting the classification of the Amur, Bengal, Indochinese, Malayan, Sumatran, and South China tigers as unique subspecies. The results inform both in situ and ex situ conservation efforts by clarifying population structure and evolutionary relationships among tigers.