S tudent Name __________________________ Date____________ LAB REPORT EVALUATION MATRIX (Biology 156) GOOD AVERAGE POOR NOT PRESENT (BUT NEEDED) ABSTRACT to include the following (10 pts) 250 words or less _______ ________ _______ _______ problem investigated _______ ________ _______ _______ methods _______ ________ _______ _______ major results _______ ________ _______ _______ conclusions _______ ________ _______ _______ significance of findings _______ ________ _______ _______ TOTAL _______ INTRODUCTION to include the following (20 pts) sufficient background info on organism/system with citations _______ ________ _______ _______ problem clearly defined _______ ________ _______ _______ purpose and goals _______ ________ _______ _______ hypothesis/prediction _______ ________ _______ _______ TOTAL _______ MATERIALS AND METHODS to include the following (20 pts) summary of process _______ ________ _______ _______ purpose of a procedure _______ ________ _______ _______ no excess mundane details _______ ________ _______ _______ TOTAL _______ RESULTS to include the following (15 pts) Tables and graphs clearly labeled _______ ________ _______ _______ Narrative includes summary tables and graphs _______ ________ _______ _______ TOTAL _______ DISCUSSION to include the following (20 pts) interpretation of results _______ ________ _______ _______ Explanation of significance of results _______ ________ _______ _______ has related discussion of results to the goals, hypothesis and purpose of experiment, including conclusions _______ ________ _______ _______ includes citations _______ ________ _______ _______ TOTAL _______ LITERATURE CITED to include the following (5 pts) listed alphabetically _______ ________ _______ _______ cited properly within narrative of paper _______ ________ _______ _______ correct format of citation _______ ________ _______ _______ TOTAL _______ WRITTEN EFFECTIVELY to include the following (10 pts) written clearly _______ ________ _______ _______ Title page _______ ________ _______ _______ use of metric system _______ ________ _______ _______ each paragraph conveys a single major idea and has topic sentence at beginning _______ ________ _______ _______ pages numbered _______ ________ _______ _______ avoidance of I and me statements _______ ________ _______ _______ TOTAL _______ Lab Report Grade ________ Using Transpirometers Must be put in your own word Preparing the Plant (this can be done hours before the experiment is to be conducted): 1. Select a plant that has a section of stem that is of slightly larger diameter than the tan rubber tubing attached to the pipette tip. 2. Cut the plant off at its base, below where the diameter is slightly larger than the rubber tubing. Immerse cute end in pan of water. 3. Immerse the pipette with rubber tubing attached in the pan of water to remove all air from the pipette and tubing. This may require pinching and squeezin.

1. S
tudent Name __________________________
Date____________
LAB REPORT EVALUATION MATRIX (Biology 156)
GOOD AVERAGE POOR NOT PRESENT
(BUT NEEDED)
ABSTRACT to include the following (10 pts)
250 words or less _______ ________ _______ _______
problem investigated _______ ________ _______ _______
methods _______ ________ _______ _______
major results _______ ________ _______ _______
conclusions _______ ________ _______ _______
significance of findings _______ ________ _______ _______
TOTAL _______
INTRODUCTION to include the following (20 pts)
sufficient background info on organism/system

2. with citations _______ ________ _______ _______
problem clearly defined _______ ________ _______ _______
purpose and goals _______ ________ _______ _______
hypothesis/prediction _______ ________ _______ _______
TOTAL _______
MATERIALS AND METHODS to include the following (20 pts)
summary of process _______ ________ _______ _______
purpose of a procedure _______ ________ _______ _______
no excess mundane details _______ ________ _______ _______
TOTAL _______
RESULTS to include the following (15 pts)
Tables and graphs clearly labeled _______ ________ _______
_______
Narrative includes summary tables and graphs _______
________ _______ _______
TOTAL _______
DISCUSSION to include the following (20 pts)

3. interpretation of results _______ ________ _______ _______
Explanation of significance of results _______ ________
_______ _______
has related discussion of results to the goals,
hypothesis and purpose of experiment, including
conclusions _______ ________ _______ _______
includes citations _______ ________ _______ _______
TOTAL _______
LITERATURE CITED to include the following (5 pts)
listed alphabetically _______ ________ _______ _______
cited properly within narrative of paper _______ ________
_______ _______
correct format of citation _______ ________ _______ _______
TOTAL _______
WRITTEN EFFECTIVELY to include the following (10 pts)
written clearly _______ ________ _______ _______
Title page _______ ________ _______ _______
use of metric system _______ ________ _______ _______

4. each paragraph conveys a single major idea and has
topic sentence at beginning _______ ________ _______
_______
pages numbered _______ ________ _______ _______
avoidance of I and me statements _______ ________ _______
_______
TOTAL _______
Lab Report Grade ________
Using Transpirometers Must be put in your own word
Preparing the Plant (this can be done hours before the
experiment is to be conducted):
1. Select a plant that has a section of stem that is of slightly
larger diameter than the tan
rubber tubing attached to the pipette tip.
2. Cut the plant off at its base, below where the diameter is
slightly larger than the rubber
tubing. Immerse cute end in pan of water.
3. Immerse the pipette with rubber tubing attached in the pan of
water to remove all air from

5. the pipette and tubing. This may require pinching and squeezing
the tubing. All air
bubbles must be removed.
4. With the stem submerged, cut the plant again at the point
where the stem is slightly larger
than the rubbing tubing. Keep the cut stem under water at all
times.
5. Slide the rubber tubing over the cut stem.
6. Hold the plant and tubing out the water and check for leaks.
If air percolates into the tip
of the pipette right away then there is a leak. If there is a leak,
resubmerge and try sliding
the tubing onto the stem farther.
Preparing the Apparatus:
1. Flush the system by opening the screw valve below the
reservoir. Flush until no bubbles
leave the micropipette then clamp off the micropipette using the
spring clamp. Continue
flushing until no bubbles are present in the line where the plant
will be inserted.
2. Insert the plant and pipette combination into the tube. Wrap a
paper towel around the
stem of the plant and insert the plant into the open clamp.

6. Clamp just enough to hold the
plant upright.
3. Open the spring clamp on the line leading to the
micropipette.
4. Open the screw valve on the reservoir just enough for water
to drip from the micropipette
and then close the screw valve. Blot the drop from the tip of the
pipette.
5. If the leaves of the plant are very wet, gently blot them dry.
6. Transpiration should be noticeable within a few minutes as
water is drawn from the
micropipette.
Measuring Transpiration Rates:
1. The micropipette is marked at the 100 μL point. Students can
measure the length to the
mark (usually about 88 mm) and then calculate a conversion
factor (88 mm/100 μL).
Using the conversion factor they can calculate the volume
transpired per unit time.
2. Factors that may affect transpiration rates are light, heat, and
wind. However, exposure
to extremes of heat or wind may cause the plant to close its
stomates rather than transpire

7. more. This might be something to try at the end of a lab period.
Group: Wind
Transpiration Data
Cumulative Distance (mm)
Average
Rate
Rate
Transpiration

8. 0 (min)
5 (min)
10 (min)
15 (min)
Temp ( C )
Surface Area (mm
2
)
(mm/min)
(uL/min)
(uL/min/mm2)
Control
0
1
8
13
24
7596
0.867
0.986
1.30E-04

9. Cumulative Distance (mm)
Average
Rate
Rate
Transpiration
Difference
% Difference
0 (min)
5 (min)
10 (min)
15 (min)
Temp ( C )
Surface Area (mm
2
)
(mm/min)
(uL/min)
(uL/min/mm2)
Treatment - Control
(Treatment - Control)/Control * 100

10. Wind
0
5
12
17
22
7,596
1.13
1.290
1.70E-04
4.00E-05
30.77%

11. Group: Light
Transpiration Data
Cumulative Distance (mm)
Average
Rate
Rate
Transpiration
0 (min)
5 (min)
10 (min)
15 (min)
Temp ( C )
Surface Area (mm
2
)
(mm/min)
(uL/min)
(uL/min/mm2)

12. Control
0
1
8
13
24
7596
0.867
0.986
1.30E-04
Cumulative Distance (mm)
Average
Rate
Rate
Transpiration
Difference
% Difference
0 (min)
5 (min)

13. 10 (min)
15 (min)
Temp ( C )
Surface Area (mm
2
)
(mm/min)
(uL/min)
(uL/min/mm2)
Treatment - Control
(Treatment - Control)/Control * 100
Light
0
7
16
25
23
7964
1.67
1.897
2.38E-04
1.08E-04
83.42%