The document summarizes the basic functions and components of a fermenter. It discusses the vessel construction, temperature control, aeration and agitation systems, and other factors like pH control. Proper containment and aseptic operation are important to prevent contamination. Different types of seals, impellers, spargers and other components are described that work to control the fermentation environment. The fermenter provides microorganisms with optimal conditions for growth and product formation.
This document provides information on the process of fermentation. It discusses what fermentation is, the range of fermentation processes, and the basic steps to carry out a fermentation. It also describes the basic design, components, functions, and materials used in fermenters. Specific aspects of fermenters covered include aeration and agitation systems, sterilization of air supply, types of seals, valves, and monitoring and control parts.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
This document discusses the design of large scale fermenters and their controls. It describes ideal properties for fermenters including supporting organism growth, temperature and pH control, and ease of use. The basic components of fermenters are then outlined, including their various shapes and sizes, as well as common materials like stainless steel. Monitoring and control systems are also summarized.
This document provides an overview of fermenters. It discusses that fermenters are containment systems that provide the optimal environment for microorganisms to convert a substrate into a higher value product. It describes the basic functions of fermenters and lists some common components like the vessel body, agitator, baffles and spargers. It also outlines different types of fermenters including stirred tank, airlift, packed bed and fluidized bed bioreactors. Finally, it briefly discusses types of fermentation processes based on culture methods.
This document discusses the design and construction of bioreactors. It explains that bioreactors provide optimal conditions for growing microorganisms by maintaining sterility and mixing. The key components of bioreactors include the vessel, agitator, sparger, temperature, pH and foam probes, cooling jacket, heating coil, and controls for dissolved oxygen and pressure. Proper monitoring and control of factors like temperature, pH, oxygen levels, and shear forces are necessary to support microbial growth and product formation.
Bioreactor and applications of bioreactorsAmjad Afridi
What is a bioreactor:?
An closed apparatus use for growing organisms (yeast, bacteria, or animal cells) under controlled conditions.
Used in industrial processes to produce pharmaceuticals, vaccines, or antibodies.
Also used to convert raw materials into useful byproducts such as in the bioconversion of corn into ethanol.
This document provides information on the process of fermentation. It discusses what fermentation is, the range of fermentation processes, and the basic steps to carry out a fermentation. It also describes the basic design, components, functions, and materials used in fermenters. Specific aspects of fermenters covered include aeration and agitation systems, sterilization of air supply, types of seals, valves, and monitoring and control parts.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
This document discusses the design of large scale fermenters and their controls. It describes ideal properties for fermenters including supporting organism growth, temperature and pH control, and ease of use. The basic components of fermenters are then outlined, including their various shapes and sizes, as well as common materials like stainless steel. Monitoring and control systems are also summarized.
This document provides an overview of fermenters. It discusses that fermenters are containment systems that provide the optimal environment for microorganisms to convert a substrate into a higher value product. It describes the basic functions of fermenters and lists some common components like the vessel body, agitator, baffles and spargers. It also outlines different types of fermenters including stirred tank, airlift, packed bed and fluidized bed bioreactors. Finally, it briefly discusses types of fermentation processes based on culture methods.
This document discusses the design and construction of bioreactors. It explains that bioreactors provide optimal conditions for growing microorganisms by maintaining sterility and mixing. The key components of bioreactors include the vessel, agitator, sparger, temperature, pH and foam probes, cooling jacket, heating coil, and controls for dissolved oxygen and pressure. Proper monitoring and control of factors like temperature, pH, oxygen levels, and shear forces are necessary to support microbial growth and product formation.
Bioreactor and applications of bioreactorsAmjad Afridi
What is a bioreactor:?
An closed apparatus use for growing organisms (yeast, bacteria, or animal cells) under controlled conditions.
Used in industrial processes to produce pharmaceuticals, vaccines, or antibodies.
Also used to convert raw materials into useful byproducts such as in the bioconversion of corn into ethanol.
This PPT dicusses about the Stirred Tank Bioreactor and its features mainly used in Fermentation process.
Useful for students doing their Bachelor's in Life Science
bioprocess and industrial biotechnology.pptxMelvinM11
1. Bioreactors are engineered devices that support biologically active environments. They control factors like temperature, pH, aeration and agitation to optimize microbial growth.
2. Early bioreactors from the 1940s were used to produce yeast and acetone on large scales. Advances in design incorporated mixing, aeration, heat transfer and sterilization systems.
3. Bioreactors come in various types including continuous stirred tank, bubble column, airlift and others. Each type aims to efficiently transfer gases, heat and momentum between liquid and gas phases.
The document discusses different types of fermenters used in biological processes. It explains that fermenters provide an optimal environment for microorganisms to interact with substrates and form desired products. There are two main types - open and closed fermenters. Key requirements for fermenters include maintaining sterile conditions, effective mixing through aeration and agitation, and monitoring environmental factors like pH, temperature and dissolved oxygen. Common mixing mechanisms used are disc turbines, vaned discs, and propellers attached to agitator shafts. Spargers are also discussed for introducing air into the fermentation broth.
The document discusses several air sampling devices used to collect airborne particles such as microorganisms, pollen, and spores. The slit sampler uses a rotating petri dish under a slit to directly impinge particles onto agar media. The Anderson sampler uses progressively smaller perforations to increase air speed and momentum to collect particles of different sizes on agar plates. The Burkard spore trap is a volumetric sampler that uses an adhesive tape to continuously trap particles over 24 hours to 1 week to analyze pollen and spore levels over time.
PRESSURE VESSELS Presentation Codes And StandardsZainSalleh1
Pressure vessels are containers designed to hold liquids and gases under pressure. They are regulated by ASME codes and come in different shapes depending on their application. Common components include cylindrical shells, domed heads, and supporting legs or skirts. Proper venting and relief devices are required for safety. Heat transfer surfaces and cleaning systems like CIP/SIP are also important design considerations for some vessel uses.
A fermentor, also known as a bioreactor, is a closed vessel used for commercial fermentation processes. It provides controls for factors like temperature, pH, aeration and agitation to maintain optimal conditions for microbial growth. Early large-scale fermentors had capacities over 20 liters and were used to produce products like yeast and acetone. Modern fermentors can be designed as various types depending on the application, including stirred tank, airlift, photo and fluidized/packed bed bioreactors. Proper design of components like the vessel material, agitator, sparger and temperature/pH controls is important for efficient fermentation.
This document discusses the airlift fermenter. It notes that fermenters must provide a controlled environment for microorganism or cell growth to produce desired products. An airlift fermenter circulates liquid using the density difference between the riser and downcomer columns caused by sparged air or gas. The main type discussed is the concentric draft tube airlift fermenter, which has an internal riser tube that introduces gas to lift liquid up the riser and down the surrounding downcomer tube. Tower loop and ICI deep shaft airlift fermenters are also mentioned. Airlift fermenters provide mixing without mechanical agitation and have high oxygen transfer rates, making them well-suited
This document provides details on the key components and functioning of a stirred tank bioreactor. It describes the standard geometry of bioreactors including dimensions. It outlines the basic features of a bioreactor including the agitation system, oxygen delivery system, temperature and pH control systems, and cleaning facilities. Specifics are provided on impeller types, mechanical seals, air sterilization methods, positive pressure maintenance, and spargers.
This document provides information on different types of bioreactors. It begins by defining a bioreactor as a vessel that enables microbial growth while preventing contamination and providing necessary conditions. It then describes six main types of bioreactors: stirred tank, bubble column, airlift, fluidized bed, packed bed, and photobioreactor. Each type is discussed in 1-2 paragraphs, outlining its mixing method, applications, and basic design. Key parts of bioreactors like temperature control, pH control, and foam control systems are also summarized. The document concludes by stating that bioreactors must carefully control factors like oxygen delivery, agitation, temperature, pH, and foam to optimize microbial production.
This document provides information about the basic components and functioning of an anaesthesia machine. It discusses the key components of the machine's pneumatic and electrical systems. The pneumatic system includes the high pressure, intermediate pressure and low pressure systems which are responsible for delivering precisely controlled gas mixtures from pressurized cylinders or central pipelines. The electrical components power and monitor the machine. The document also provides details on cylinders, pressure regulators and other individual parts that make up the overall anaesthesia machine.
The document discusses the components and functioning of an anaesthesia work station's high pressure system. It describes the key components including gas cylinders, hanger yokes, cylinder pressure indicators, and pressure regulators. Gas cylinders contain medical gases at high pressure and have valves, handles, pressure relief devices, and markings. Hanger yokes orient and secure cylinders, providing a gas-tight seal. Cylinder pressure indicators display the pressure level in cylinders. Pressure regulators reduce the high cylinder pressure to a lower, constant pressure suitable for use in the anaesthesia machine.
This document provides information about different types of valves used in industrial processes. It describes check valves, diaphragm valves, solenoid valves, butterfly valves, and pressure relief valves. For each type of valve, it discusses their description, function, applications, and includes diagrams. Videos are also referenced for additional information.
The document provides instructions for cleaning an anaerobic digester tank. Key steps include isolating the digester, removing as much material as possible, ventilating the space, following strict safety protocols like lock out tag out and confined space entry procedures, and pumping out remaining sludge and inert materials. The pumped sludge can be sent to grit removal, screening, storage tanks for dewatering, or direct land application depending on the disposal option selected. Reseeding from another source or slowly building up biomass over time are described as options for restarting digestion after cleaning is complete.
Oxygen MANUFACTRE STORAGE PREPERATION AND CLINICAL ASPECTDr.RMLIMS lucknow
Oxygen is produced primarily through two main methods - fractional distillation of air and pressure swing absorption. It is stored in large bulk systems or compressed gas cylinders. Cylinders come in various standardized sizes and have safety features like pressure relief valves and color coding. Oxygen is delivered to patients through devices like nasal cannulas, masks, or venturi masks which mix oxygen with air to precisely control the fraction of inspired oxygen. While oxygen therapy is useful for treating hypoxemia, high concentrations over long periods can cause toxicity issues like pulmonary fibrosis or retinopathy of prematurity in newborns.
The document describes different types of fermenters used in bioprocessing, including stirred tank, airlift, and tower fermenters. Key components of fermenters include agitation systems to mix and oxygenate the culture, as well as systems to control temperature, pH, foam, and sterilization. Fermenters are made of glass or stainless steel and include features like spargers, impellers, and probes to monitor conditions for microbial growth and product formation. Larger fermenters are often used in two-stage configurations that allow cultures to be transferred between vessels with different temperature or process conditions.
This document describes several vessel designs for fermentation processes, including air lift fermentors, fluidized bed fermentors, hollow fiber fermentors, and in situ-sterilizable fermentors. Air lift fermentors mix and aerate culture fluid using an upward air stream and have applications for shear-sensitive and high biomass cultures. Fluidized bed fermentors recirculate medium and are well-suited for animal cell cultures. Hollow fiber fermentors embed cells in permeable fibers to exchange gases and metabolites between vessels. In situ-sterilizable fermentors can be fully sterilized using steam heating within the vessel.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
This document discusses aeration and agitation in bioreactors. It describes how aeration supplies oxygen for microbial metabolism while agitation mixes nutrients so microbes can access them. Structural components like impellers, baffles, and spargers are explained. Disc, vane, and variable pitch turbines as well as propellers are classified as agitator types. The document also details stuffing box, mechanical, and magnetic seals for agitator shafts. Finally, porous, orifice, and nozzle spargers are compared for introducing air into the bioreactor medium.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
More Related Content
Similar to STRUCTURE & APPLICATIONS OF A LABORATORY BIOREACTOR.pptx
This PPT dicusses about the Stirred Tank Bioreactor and its features mainly used in Fermentation process.
Useful for students doing their Bachelor's in Life Science
bioprocess and industrial biotechnology.pptxMelvinM11
1. Bioreactors are engineered devices that support biologically active environments. They control factors like temperature, pH, aeration and agitation to optimize microbial growth.
2. Early bioreactors from the 1940s were used to produce yeast and acetone on large scales. Advances in design incorporated mixing, aeration, heat transfer and sterilization systems.
3. Bioreactors come in various types including continuous stirred tank, bubble column, airlift and others. Each type aims to efficiently transfer gases, heat and momentum between liquid and gas phases.
The document discusses different types of fermenters used in biological processes. It explains that fermenters provide an optimal environment for microorganisms to interact with substrates and form desired products. There are two main types - open and closed fermenters. Key requirements for fermenters include maintaining sterile conditions, effective mixing through aeration and agitation, and monitoring environmental factors like pH, temperature and dissolved oxygen. Common mixing mechanisms used are disc turbines, vaned discs, and propellers attached to agitator shafts. Spargers are also discussed for introducing air into the fermentation broth.
The document discusses several air sampling devices used to collect airborne particles such as microorganisms, pollen, and spores. The slit sampler uses a rotating petri dish under a slit to directly impinge particles onto agar media. The Anderson sampler uses progressively smaller perforations to increase air speed and momentum to collect particles of different sizes on agar plates. The Burkard spore trap is a volumetric sampler that uses an adhesive tape to continuously trap particles over 24 hours to 1 week to analyze pollen and spore levels over time.
PRESSURE VESSELS Presentation Codes And StandardsZainSalleh1
Pressure vessels are containers designed to hold liquids and gases under pressure. They are regulated by ASME codes and come in different shapes depending on their application. Common components include cylindrical shells, domed heads, and supporting legs or skirts. Proper venting and relief devices are required for safety. Heat transfer surfaces and cleaning systems like CIP/SIP are also important design considerations for some vessel uses.
A fermentor, also known as a bioreactor, is a closed vessel used for commercial fermentation processes. It provides controls for factors like temperature, pH, aeration and agitation to maintain optimal conditions for microbial growth. Early large-scale fermentors had capacities over 20 liters and were used to produce products like yeast and acetone. Modern fermentors can be designed as various types depending on the application, including stirred tank, airlift, photo and fluidized/packed bed bioreactors. Proper design of components like the vessel material, agitator, sparger and temperature/pH controls is important for efficient fermentation.
This document discusses the airlift fermenter. It notes that fermenters must provide a controlled environment for microorganism or cell growth to produce desired products. An airlift fermenter circulates liquid using the density difference between the riser and downcomer columns caused by sparged air or gas. The main type discussed is the concentric draft tube airlift fermenter, which has an internal riser tube that introduces gas to lift liquid up the riser and down the surrounding downcomer tube. Tower loop and ICI deep shaft airlift fermenters are also mentioned. Airlift fermenters provide mixing without mechanical agitation and have high oxygen transfer rates, making them well-suited
This document provides details on the key components and functioning of a stirred tank bioreactor. It describes the standard geometry of bioreactors including dimensions. It outlines the basic features of a bioreactor including the agitation system, oxygen delivery system, temperature and pH control systems, and cleaning facilities. Specifics are provided on impeller types, mechanical seals, air sterilization methods, positive pressure maintenance, and spargers.
This document provides information on different types of bioreactors. It begins by defining a bioreactor as a vessel that enables microbial growth while preventing contamination and providing necessary conditions. It then describes six main types of bioreactors: stirred tank, bubble column, airlift, fluidized bed, packed bed, and photobioreactor. Each type is discussed in 1-2 paragraphs, outlining its mixing method, applications, and basic design. Key parts of bioreactors like temperature control, pH control, and foam control systems are also summarized. The document concludes by stating that bioreactors must carefully control factors like oxygen delivery, agitation, temperature, pH, and foam to optimize microbial production.
This document provides information about the basic components and functioning of an anaesthesia machine. It discusses the key components of the machine's pneumatic and electrical systems. The pneumatic system includes the high pressure, intermediate pressure and low pressure systems which are responsible for delivering precisely controlled gas mixtures from pressurized cylinders or central pipelines. The electrical components power and monitor the machine. The document also provides details on cylinders, pressure regulators and other individual parts that make up the overall anaesthesia machine.
The document discusses the components and functioning of an anaesthesia work station's high pressure system. It describes the key components including gas cylinders, hanger yokes, cylinder pressure indicators, and pressure regulators. Gas cylinders contain medical gases at high pressure and have valves, handles, pressure relief devices, and markings. Hanger yokes orient and secure cylinders, providing a gas-tight seal. Cylinder pressure indicators display the pressure level in cylinders. Pressure regulators reduce the high cylinder pressure to a lower, constant pressure suitable for use in the anaesthesia machine.
This document provides information about different types of valves used in industrial processes. It describes check valves, diaphragm valves, solenoid valves, butterfly valves, and pressure relief valves. For each type of valve, it discusses their description, function, applications, and includes diagrams. Videos are also referenced for additional information.
The document provides instructions for cleaning an anaerobic digester tank. Key steps include isolating the digester, removing as much material as possible, ventilating the space, following strict safety protocols like lock out tag out and confined space entry procedures, and pumping out remaining sludge and inert materials. The pumped sludge can be sent to grit removal, screening, storage tanks for dewatering, or direct land application depending on the disposal option selected. Reseeding from another source or slowly building up biomass over time are described as options for restarting digestion after cleaning is complete.
Oxygen MANUFACTRE STORAGE PREPERATION AND CLINICAL ASPECTDr.RMLIMS lucknow
Oxygen is produced primarily through two main methods - fractional distillation of air and pressure swing absorption. It is stored in large bulk systems or compressed gas cylinders. Cylinders come in various standardized sizes and have safety features like pressure relief valves and color coding. Oxygen is delivered to patients through devices like nasal cannulas, masks, or venturi masks which mix oxygen with air to precisely control the fraction of inspired oxygen. While oxygen therapy is useful for treating hypoxemia, high concentrations over long periods can cause toxicity issues like pulmonary fibrosis or retinopathy of prematurity in newborns.
The document describes different types of fermenters used in bioprocessing, including stirred tank, airlift, and tower fermenters. Key components of fermenters include agitation systems to mix and oxygenate the culture, as well as systems to control temperature, pH, foam, and sterilization. Fermenters are made of glass or stainless steel and include features like spargers, impellers, and probes to monitor conditions for microbial growth and product formation. Larger fermenters are often used in two-stage configurations that allow cultures to be transferred between vessels with different temperature or process conditions.
This document describes several vessel designs for fermentation processes, including air lift fermentors, fluidized bed fermentors, hollow fiber fermentors, and in situ-sterilizable fermentors. Air lift fermentors mix and aerate culture fluid using an upward air stream and have applications for shear-sensitive and high biomass cultures. Fluidized bed fermentors recirculate medium and are well-suited for animal cell cultures. Hollow fiber fermentors embed cells in permeable fibers to exchange gases and metabolites between vessels. In situ-sterilizable fermentors can be fully sterilized using steam heating within the vessel.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
These are the sterile preparation intended to administered other than intestinal route to bypass first pass metabolism and directly goes to systemic circulation.
These preparation give quick onset of action and site specific activity.
Suitable for drugs which are inactive in GIT environment.
Can be given unconscious or vomiting or diarrheal patient.
This document discusses aeration and agitation in bioreactors. It describes how aeration supplies oxygen for microbial metabolism while agitation mixes nutrients so microbes can access them. Structural components like impellers, baffles, and spargers are explained. Disc, vane, and variable pitch turbines as well as propellers are classified as agitator types. The document also details stuffing box, mechanical, and magnetic seals for agitator shafts. Finally, porous, orifice, and nozzle spargers are compared for introducing air into the bioreactor medium.
Similar to STRUCTURE & APPLICATIONS OF A LABORATORY BIOREACTOR.pptx (20)
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
Phenomics assisted breeding in crop improvementIshaGoswami9
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
The binding of cosmological structures by massless topological defectsSérgio Sacani
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Equivariant neural networks and representation theory
STRUCTURE & APPLICATIONS OF A LABORATORY BIOREACTOR.pptx
1. STRUCTURE &
APPLICATIONS OF A
FERMENTER
PRESENTED BY :
Tanishka (211206)
MSc. Biotechnology
3rd semester,2nd year
2. BASIC FUNCTIONS OF FERMENTER:
Provide a controlled environment for the growth of microorganisms
or animal cells, to obtain a desired product.
• Vessel - aseptically reliable in long-term operation
• Adequate aeration and agitation
( mixing should not cause damage to the organism nor cause excessive
foam generation).
• Power consumption - as low as possible.
• System for temperature control
• System of pH monitoring and control & other parameters (eg,
dissolved oxygen, redox, etc.)
3. • Sampling facilities
• Evaporation losses from the fermenter should not be excessive.
• Require the minimal use of labor in operation, harvesting, cleaning,
and maintenance.
• Ensure smooth internal surfaces, using welds inside the vessel
• Vessel should be of similar geometry to both smaller and larger vessels
• Use of cheapest material enabling satisfactory results
• There should be adequate service provisions for individual plants.
4.
5. ASPETIC OPERATION & CONTAINMENT
Aseptic operation protection against contamination
containment prevention of escape of viable cells
from a fermenter or downstream equipment.
To establish the appropriate degree of containment ,the entire
process must be carefully assessed for potential hazards that could
occur in case of accidental release.
6. HAZARD ASSESSMENT SYSTEM:
• Once the organism has been allocated to a hazard group, the
appropriate containment requirements can be applied.
• Hazard group 1 organisms used on a large scale only require.
• Processes in this category need to be operated aseptically but no
containment steps are necessary, including prevention of escape of
organisms.
• If the organism is placed in Hazard group 4 the stringent
requirements of level 3 will have to be met before the process can be
operated.
8. Hazard group criteria :
• known pathogenicity
• virulence or level of pathogenicity
• number of organisms required to initiate an infection.
• routes of infection.
• The amounts or volumes of organisms used in the fermentation
process etc.
9. FERMENTER BODY CONSTRUCTION - VESSEL
CHARACTERSTICS:
• Withstand pressure sterilization
• Corrosion proof
• Non-toxic
• Less cost
10. • The American Iron and Steel Institute (AISI) states that :
• Mild steel coated with glass or phenolic epoxy materials has
occasionally been used.
• Chromium provides resistance from the corrosion & the minimum
amount of chromium required depends on the corroding agent in a
particular environment, such as acids, alkalis, gases, soil, salt, or fresh
water.
Steels
<4% chromium
>4% chromium
STEEL
ALLOYS
STAINLESS
STEELS
11.
12. SEAL:
• a reliable aseptic seal is made between a fermenter vessel and a
detachable top or base plate.
• This seal ensures that a good liquid- and/or gas-tight joint is maintained in
spite of the glass or metal expanding or contracting at different rates with
changes in temperature during a sterilization cycle or an incubation cycle.
• Nitryl or butyl rubbers are normally used for these seals as they will
withstand fermentation process conditions.
• These rubber seals have a finite life and should be checked regularly for
damage or perishing.
14. TEMPERATURE CONTROL
Heat produced due to of :
• microbial activity
• mechanical agitation
Which is not ideal for the
particular manufacturing
process
So, heat may have to be added, or
removed from the system.
On laboratory scale,
addition/removal of heat occurs
by :
placing the fermenter in a
thermostatically controlled bath
use of internal heating coils
a heating jacket through which
water is circulated.
15. Accurate estimation of heating/cooling requirements:
Where
Qmet- heat generation rate due to microbial metabolism
Qag - heat generation rate due to mechanical agitation
Qgas - heat generation rate due to aeration power input
Qacc - heat accumulation rate by the system
Qexch - heat transfer rate to the surroundings and/or heat exchanger
Qevap - heat loss rate by evaporation
Qsen - rate of sensible enthalpy gain by the flow streams (exit—inlet)
16. AERATION & AGITATION
to provide microorganisms
in submerged culture with
sufficient oxygen for
metabolic requirements
ensure uniform suspension of
microbial cells is achieved in a
homogeneous nutrient
medium.
The structural components of the fermenter involved :
a. The agitator (impeller).
b. Stirrer glands and bearings.
c. Baffles.
d. The aeration system (sparger)
17. IMPELLER:
Required:
• bulk fluid and gas-phase mixing
• oxygen transfer
• heat transfer
• suspension of solid particles
• maintaining a uniform environment throughout the vessel contents.
18. • Disc turbines: They comprise
disc with a set of rectangular
vanes. They allow an air stream
from the sparger to strike on the
disc’s underside and then move
the air toward the vanes,
breaking large air bubbles down
into smaller ones.
• Variable pitch open
turbine: They also comprise a
an agitator shaft that is vanned
and joined to propeller blades of
the marine on the shaft for the
agitator. The air bubbles that
make up this turbine don’t touch
any surface prior to dispersing
19. STIRRER GLANDS & BEARINGS:
• The satisfactory sealing of the stirrer shaft assembly top
plate has been one of the most difficult problems to
overcome in the construction of fermentation equipment
which can be operated aseptically for long periods.
• The stirrer shaft can enter the vessel from the top, side or
bottom of the vessel.
20.
21. EARLIEST STIRRERS SEAL :
• A porous bronze bearing for a 13-mm shaft was fitted in
the centre of the fermenter top and another in a yoke
directly above it.
• The bearings were pressed into steel housings, which
screwed into position in the yoke and the fermenter top.
• The lower bearing and housing were covered with a skirt-
like shield having a 6.5 mm overhang which rotated with
the shaft and prevented air- borne contaminants from
settling on the bearing and working their way through it
into the fermenter.
22. Four basic types of seal assembly have been used:
• the stuffing box (packed-gland seal)
The shaft is sealed by several layers of packing rings of asbestos or
cotton yarn, pressed against the shaft by a gland follower
• the simple bush seal
• the mechanical seal
• the magnetic drive
Most modern fermenter stirrer mechanisms now incorporate
mechanical seals instead of stuffing boxes and packed glands.
23. Mechanical seals are more expensive, but
are more durable and less likely to be an
entry point for contaminants or a leakage
point for organisms or products which
should be contained
Seal is composed of two parts, one part is stationary in
the bearing housing, the other rotates on the shaft, and
the two components are pressed together by springs or
expanding bellows.
The two meeting surfaces have to be precision
machined, the moving surface normally consists of a
carbon-faced unit while the stationary unit is of stellite-
faced stainless steel.
24. Magnetic drives, which are also quite
expensive, have been used in animal cell
culture vessels.
The problems of providing a satisfactory seal
when the impeller shaft passes through the top
or bottom plate of the fermenter may be solved
by the use of a magnetic drive in which the
impeller shaft does not pierce the vessel.
• A magnetic drive consists of two magnets:
one driving and one driven.
25. BAFFLES :
• Four baffles are normally incorporated into agitated vessels of all sizes
to prevent a vortex & to prevent aeration efficiency.
• In vessels over 3-dm3 six or eight baffles may be used.
• Baffles are metal strips roughly one-tenth of the vessel diameter and
attached radially to the wall
• The agitation effect is only slightly increased with wider baffles, but
drops sharply with narrower baffles.
26.
27.
28. SPARGER:
• a device for introducing air into the liquid in a fermenter.
• A combined sparger-agitator may be used in laboratory fermenter.
• Three basic types of sparger:
• the porous sparger
• the orifice sparger (a perforated pipe)
• the nozzle sparger (an open or partially closed pipe)
29. POROUS SPARGER:
The porous sparger of sintered glass, ceramics or metal, has
been used primarily on a laboratory scale in non-agitated
vessels.
• The bubble size produced from such spargers is always 10
to 100 times larger than the pore size of the aerator block.
• There is also the problem of the fine holes becoming
blocked by growth of the microbial culture.
30. ORIFICE SPARGER:
• In small stirred fermenters the perforated pipes were
arranged below the impeller in the form of crosses or rings
(ring sparger), approximately three-quarters of the impeller
diameter.
• In most designs the air holes were drilled on the under
surfaces of the tubes making up the ring or cross.
31. NOZZLE SPARGER:
• Single open or partially closed pipe as a sparger to provide
the stream of air bubbles.
• Ideally the pipe should be positioned centrally below the
impeller and as far away as possible from it to ensure that
the impeller is not flooded by the air stream.
32. COMBINED SPARGER
AGITATOR:
• introducing the air via a hollow agitator
shaft and emitting it through holes drilled
in the disc.
• The design gives good aeration in a baffled
vessel when the agitator is operated at a
range of rpm.
33. FEED PORTS :
• to add ingredients at the right times.
• to monitor fermentation process continuously and makes it
easy to add nutrients or remove byproducts.
• consist of tubes made of silicone.
• In-situ sterilization is carried out prior to either the removal or
the addition of ingredients.
34. FOAM CONTROL :
• volume of foam within the vessel must be reduced to prevent
contamination.
• The level of foam can be controlled with two components:
foam sensing and control.
• In the fermenter the probe is placed through the top and is set
to a certain level that is above the surface of the broth.
• If the level of foam rises and it touches the probe’s tip there will
be a current carried across the circuit.
• The current will activate the pump, and antifoam will be
released immediately to fight the issue.
35. VALVES
• Valves are employed in the fermenter for controlling the flow of
liquid inside the vessel.
• There are around five kinds of valves :
• Globe valves can be used for general use, but they don’t control
flow.
• Butterfly valves are not appropriate for use in aseptic conditions.
They are utilized for pipes with large diameters that operate at low
pressure.
• Ball valves can be used in aseptic conditions. They can handle
mycelial broths and operate at a high temperatures.
• Diaphragm valves aid in adjusting flow.
36. SAFETY VALVE:
• The safety valve is integrated into the pipe and air layout to
function under pressure. Through these valves, the pressure
remains within the safe boundaries.
37. CONTROLLING DEVICES FOR
ENVIRONMENTAL FACTORS :
• Bioreactor design must consider many parameters such as
temperature, pH, dissolved oxygen and carbon dioxide
concentrations.
• all be controlled at certain levels during the process.
• will control growth, reduce contamination, improve production
rate and increase product-quality.
• These devices will enable us to monitor the temperature,
carbon dioxide, oxygen concentration, and pH of the reactor at
any time.
40. REFERENCES:
• PRINCIPLES OF FERMENTATION TECHNOLOGY BY PETER F.STANBURY,
ALLAN WHITAKER & STEPHEN J. HALL
• https://microbenotes.com/bioreactor/#applications-of-bioreactor