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Strain improvement
• There are two distinct approaches for improvement of strains-
mutation, recombination and recombinant DNA technology.
• Random mutagenesis (classical strain
improvement)
• used extensively in the food industry
• This approach is based on the introduction of
random mutations into the genome of interest,
characterization of a large subset of variants,
and selection of strains with the desired
property for further use.
• apart from the desired mutation, many
unintended mutations which could have a
negative impact on performance are
introduced
Directed evolution
• is a technique in which a strain is slowly
adapted to certain growth conditions
reflecting an application parameter.
• In this case the population is enriched for
strains with the desired property but, here
too, there is a risk of accumulation of
unintended mutations

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strain improvement-ppt unit 2.pptx

  • 2. • There are two distinct approaches for improvement of strains- mutation, recombination and recombinant DNA technology.
  • 3. • Random mutagenesis (classical strain improvement) • used extensively in the food industry • This approach is based on the introduction of random mutations into the genome of interest, characterization of a large subset of variants, and selection of strains with the desired property for further use. • apart from the desired mutation, many unintended mutations which could have a negative impact on performance are introduced
  • 4. Directed evolution • is a technique in which a strain is slowly adapted to certain growth conditions reflecting an application parameter. • In this case the population is enriched for strains with the desired property but, here too, there is a risk of accumulation of unintended mutations