Okta Yosiana Dewi DIII Analis Kesehatan POLTEKKES SEMARANG 2014 insyaALLAH d4 Radiologi semarang amin

1. Stining gram-negative
OKTA YOSI ANA DEWI
REG.B / SMT I I

2. How to make a stain on gram-negative bacteria
1. Prepare tools and materials
Tools :
- Ose - Lighter
- Bunsen

3. - Pasteur pipette
- Cotton - Glass Objects

4. • material:
- Bacteria - 70% Alcohol
- Tinta Cina

5. 2. Use of personal protective equipment (PPE) such
as :
a.Lab coats
b. Masks
c. Handscoon

6. 3. After that the first thing to do is
glass objects in the given cotton that have
been given alcohol and then in fixation

7. 4. The second loop is heated in a Bunsen that
subsequent sterile bacteria in open
 Bacteria in the open in near Bunsen to remain
sterile

8. 5. And then bacterial colonies were taken and
placed in glass object then fixed again

9. 6. After fixation is complete, thenChinese ink
drops on a glass object and in lear glass object
by pressing and pushing forward
 Prepared smears examined in the microscope