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Separation Techniques
Kittitat Kanta-anantapornBy…
Adsorption is the adhesion of atoms,
ions, or molecules from a gas, liquid, or
dissolved solid to a surface. This process
creates a film of the adsorbate on the
surface of the adsorbent. This process
differs from absorption, in which a fluid
(the absorbate) is dissolved by or
permeates a liquid or solid (the
absorbent), respectively
1. Adsorbtion
Capillary electrophoresis (CE) is
a family of electrokinetic separation
methods performed in submillimeter
diameter capillaries and in micro
and nanofluidic channels. Very
often, CE refers to capillary zone
electrophoresis (CZE), but other
electrophoretic techniques including
capillary gel electrophoresis (CGE),
capillary isoelectric focusing (CIEF),
capillary isotachophoresis and
2. Capillary electrophoresis
Centrifugation is a process which involves the
application of the centripetal force for the sedimentation
of heterogeneous mixtures with a centrifuge, and is used
in industrial and laboratory settings. This process is used
to separate two miscible substances, but also to analyze
the hydrodynamic properties of macromolecules.
3. Centrifugation
4. Chromatography
Chromatography is the collective term
for a set of laboratory techniques for the
separation of mixtures. The
mixture is dissolved in a fluid called the
mobile phase, which carries it through
a structure holding another
material called the stationary phase.
The various constituents of the mixture
travel at different speeds,
causing them to separate.
The separation is based on differential
partitioning between the mobile and
Filtration is any of various mechanical, physical
or biological operations that separate solids from
fluids (liquids or gases) by adding a medium
through which only the fluid can pass. The fluid
that passes through is called the filtrate. However,
the separation is not complete; solids will be
5. Filtration
Crystallization is the (natural or artificial)
process where a solid forms where the atoms
or molecules are highly organized in a
structure known as a crystal. Some of the
ways which crystals form are through
precipitating from a solution, melting or
more rarely deposition directly from a gas.
Crystallization is also a chemical solid–liquid
separation technique, in which mass transfer
of a solute from the liquid solution to a pure
solid crystalline phase occurs. In chemical
6. Crystallization
Evaporation is a type of vaporization of a
liquid that occurs from the surface of a liquid
into a gaseous phase that is not saturated
with the evaporating substance. The other
type of vaporization is boiling, which is
characterized by bubbles of saturated vapor
forming in the liquid phase. Steam produced
in a boiler is another example of evaporation
occurring in a saturated vapor phase.
Evaporation that occurs directly from the
solid phase below the melting point, as
7. Evaporation
Decantation is a process for the
separation of mixtures, by removing a
layer of liquid, generally one from
which a precipitate has settled. The
purpose may be either to produce a
clean decant, or to remove undesired
liquid from the precipitate (or other
layers). If the aim is to produce a
clean solution, a small amount of
solution must generally be left in the
container, and care must be taken to
8. Decantation
Demister (vapor) is a device
often fitted to vapor-liquid
separator vessels to enhance the
removal of liquid droplets
entrained in a vapor stream.
Demisters may be a mesh type
coalescer, vane pack or other
structure intended to aggregate
the mist into droplets that are
heavy enough to separate from
9. Demister (vapor)
Distillation is a process of separating
the component or substances from a
liquid mixture by selective evaporation
and condensation. Distillation may result
in essentially complete separation (nearly
pure components), or it may be a partial
separation that increases the
concentration of selected components of
the mixture. In either case the process
exploits differences in the volatility of the
mixture's components. In industrial
chemistry, distillation is a unit operation of
practically universal importance, but it is a
10. Distillation
Drying is a mass transfer process consisting of the removal of water or
another solvent[1] by evaporation from a solid, semi-solid or liquid. This process
is often used as a final production step before selling or packaging products. To
be considered "dried", the final product must be solid, in the form of a
continuous sheet (e.g., paper), long pieces (e.g., wood), particles (e.g., cereal
grains or corn flakes) or powder (e.g., sand, salt, washing powder, milk powder).
A source of heat and an agent to remove the vapor produced by the process
are often involved. In bioproducts like food, grains, and pharmaceuticals like
vaccines, the solvent to be removed is almost invariably water. Desiccation may
11. Drying
Electrophoresis is the motion
of dispersed particles relative to
a fluid under the influence of a
spatially uniform electric field.
Electrophoresis is a technique
used in laboratories in order to
separate macromolecules based
on size. The
technique applies a negative
charge so proteins move
towards a positive charge. This is
12. Electrophoresis
Elutriation is a process for separating
particles based on their size, shape and density,
using a stream of gas or liquid flowing in a
direction usually opposite to the direction of
sedimentation. This method is mainly used for
particles smaller than 1 μm. The smaller or
lighter particles rise to the top (overflow)
because their terminal sedimentation velocities
are lower than the velocity of the rising fluid.
The terminal velocity of any particle in any
medium can be calculated using Stokes' Law if
the particle's Reynolds number is below 0.2.
13. Elutriation
Extraction in chemistry is a separation process consisting in the
separation of a substance from a matrix. It includes Liquid-liquid
extraction, and Solid phase extraction.
14. Extraction
Field flow fractionation
bbreviated FFF, is a separation
technique where a field is
applied to a fluid suspension or
solution pumped through a long
and narrow channel,
perpendicular to the direction of
flow, to cause separation of the
particles present in the fluid,
depending on their differing
"mobilities" under the force
15. Field flow fractionation
Froth flotation is a process
for selectively separating
hydrophobic materials from
hydrophilic. This is used in
mineral processing, paper
recycling and waste-water
treatment industries. Historically
this was first used in the mining
industry, where it was one of the
great enabling technologies of
the 20th century. It has been
16. Froth flotation
Flocculation is a process wherein colloids come out of
suspension in the form of floc or flake; either spontaneously or due
to the addition of a clarifying agent. The action differs from
precipitation in that, prior to flocculation, colloids are merely
suspended in a liquid and not actually dissolved in a solution. In the
flocculated system, there is no formation of a cake, since all the flocs
are in the suspension.
17. Flocculation
Fractional freezing is a
misnomer, since it is not
distillation but rather a process
of enriching a solution by
partially freezing it and
removing frozen material that is
poorer in the dissolved material
than is the liquid portion left
behind. Such enrichment
parallels enrichment by true
distillation, where the
18. Fractional freezing
API oil–water separator is
a device designed to separate
gross amounts of oil and
suspended solids from the
wastewater effluents of oil
refineries, petrochemical plants,
chemical plants, natural gas
processing plants and other
industrial oily water sources.
19. API oil–water separator
Magnetic separation is a process in which magnetically
susceptible material is extracted from a mixture using a magnetic
force. This separation technique can be useful in mining iron as it is
attracted to a magnet. In mines where wolframite was mixed with
cassiterite, such as South Crofty and East Pool mine in Cornwall or
with bismuth such as at the Shepherd and Murphy mine in Moina,
Tasmania, magnetic separation was used to separate the ores.
20. Magnetic separation
Precipitation is the creation
of a solid from a solution.
When the reaction occurs in a
liquid solution, the solid formed
is called the 'precipitate'. The
chemical that causes the solid
to form is called the
'precipitant'. Without sufficient
force of gravity (settling) to
bring the solid particles
together, the precipitate
21. Precipitation
Recrystallization is a technique
used to purify chemicals. By
dissolving both impurities and a
compound in an appropriate solvent,
either the desired compound or
impurities can be coaxed out of
solution, leaving the other behind. It
is named for the crystals often
formed when the compound
precipitates out. Alternatively,
recrystallization can refer to the
natural growth of larger ice crystals at
22. Recrystallization
Scrubber systems are a diverse group of air pollution control
devices that can be used to remove some particulates and/or gases
from industrial exhaust streams. The first air scrubber was designed
to remove carbon dioxide from the air of an early submarine, the
Ictineo I, a role for which they continue to be used to this day
23. Scrubber
Sedimentation is the tendency
for particles in suspension to
settle out of the fluid in which they
are entrained and come to rest
against a barrier. This is due to
their motion through the fluid in
response to the forces acting on
them: these forces can be due to
gravity, centrifugal acceleration, or
electromagnetism. In geology,
sedimentation is often used as the
24. Sedimentation
Sieve A sieve, or sifter, is a device for
separating wanted elements from
unwanted material or for characterizing
the particle size distribution of a sample,
typically using a woven screen such as a
mesh or net or metal.[1] The word "sift"
derives from "sieve". In cooking, a sifter is
used to separate and break up clumps in
dry ingredients such as flour, as well as
to aerate and combine them. A strainer
is a form of sieve used to separate solids
from liquid.
25. Sieve
Stripping is a physical
separation process where one or
more components are removed
from a liquid stream by a vapor
stream. In industrial applications
the liquid and vapor streams can
have co-current or
countercurrent flows. Stripping is
usually carried out in either a
packed or trayed column.
26. Stripping
Sublimation is the
transition of a substance
directly from the solid to the
gas phase without passing
through the intermediate liquid
phase.
27. Sublimation
Vapor–liquid
separator is a vertical
vessel into which a liquid and
vapor mixture (or a flashing
liquid) is fed and wherein the
liquid is separated by gravity, falls
to the bottom of the vessel, and
is withdrawn. The vapor travels
upward at a design velocity
which minimizes the entrainment
28. vapor–liquid separator
Winnowing is an agricultural
method developed by ancient
cultures for separating grain from
chaff. It is also used to remove
weevils or other pests from stored
grain. Threshing, the loosening of
grain or seeds from the husks and
straw, is the step in the chaff-
removal process that comes
before winnowing.
29. Winnowing
Zone melting is a group of similar methods of purifying
crystals, in which a narrow region of a crystal is melted, and this
molten zone is moved along the crystal. The molten region melts
impure solid at its forward edge and leaves a wake of purer material
solidified behind it as it moves through the ingot. The impurities
concentrate in the melt, and are moved to one end of the ingot.
30. Zone melting
The end

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Separation techniques p.6

  • 2. Adsorption is the adhesion of atoms, ions, or molecules from a gas, liquid, or dissolved solid to a surface. This process creates a film of the adsorbate on the surface of the adsorbent. This process differs from absorption, in which a fluid (the absorbate) is dissolved by or permeates a liquid or solid (the absorbent), respectively 1. Adsorbtion
  • 3. Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro and nanofluidic channels. Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and 2. Capillary electrophoresis
  • 4. Centrifugation is a process which involves the application of the centripetal force for the sedimentation of heterogeneous mixtures with a centrifuge, and is used in industrial and laboratory settings. This process is used to separate two miscible substances, but also to analyze the hydrodynamic properties of macromolecules. 3. Centrifugation
  • 5. 4. Chromatography Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and
  • 6. Filtration is any of various mechanical, physical or biological operations that separate solids from fluids (liquids or gases) by adding a medium through which only the fluid can pass. The fluid that passes through is called the filtrate. However, the separation is not complete; solids will be 5. Filtration
  • 7. Crystallization is the (natural or artificial) process where a solid forms where the atoms or molecules are highly organized in a structure known as a crystal. Some of the ways which crystals form are through precipitating from a solution, melting or more rarely deposition directly from a gas. Crystallization is also a chemical solid–liquid separation technique, in which mass transfer of a solute from the liquid solution to a pure solid crystalline phase occurs. In chemical 6. Crystallization
  • 8. Evaporation is a type of vaporization of a liquid that occurs from the surface of a liquid into a gaseous phase that is not saturated with the evaporating substance. The other type of vaporization is boiling, which is characterized by bubbles of saturated vapor forming in the liquid phase. Steam produced in a boiler is another example of evaporation occurring in a saturated vapor phase. Evaporation that occurs directly from the solid phase below the melting point, as 7. Evaporation
  • 9. Decantation is a process for the separation of mixtures, by removing a layer of liquid, generally one from which a precipitate has settled. The purpose may be either to produce a clean decant, or to remove undesired liquid from the precipitate (or other layers). If the aim is to produce a clean solution, a small amount of solution must generally be left in the container, and care must be taken to 8. Decantation
  • 10. Demister (vapor) is a device often fitted to vapor-liquid separator vessels to enhance the removal of liquid droplets entrained in a vapor stream. Demisters may be a mesh type coalescer, vane pack or other structure intended to aggregate the mist into droplets that are heavy enough to separate from 9. Demister (vapor)
  • 11. Distillation is a process of separating the component or substances from a liquid mixture by selective evaporation and condensation. Distillation may result in essentially complete separation (nearly pure components), or it may be a partial separation that increases the concentration of selected components of the mixture. In either case the process exploits differences in the volatility of the mixture's components. In industrial chemistry, distillation is a unit operation of practically universal importance, but it is a 10. Distillation
  • 12. Drying is a mass transfer process consisting of the removal of water or another solvent[1] by evaporation from a solid, semi-solid or liquid. This process is often used as a final production step before selling or packaging products. To be considered "dried", the final product must be solid, in the form of a continuous sheet (e.g., paper), long pieces (e.g., wood), particles (e.g., cereal grains or corn flakes) or powder (e.g., sand, salt, washing powder, milk powder). A source of heat and an agent to remove the vapor produced by the process are often involved. In bioproducts like food, grains, and pharmaceuticals like vaccines, the solvent to be removed is almost invariably water. Desiccation may 11. Drying
  • 13. Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. Electrophoresis is a technique used in laboratories in order to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. This is 12. Electrophoresis
  • 14. Elutriation is a process for separating particles based on their size, shape and density, using a stream of gas or liquid flowing in a direction usually opposite to the direction of sedimentation. This method is mainly used for particles smaller than 1 μm. The smaller or lighter particles rise to the top (overflow) because their terminal sedimentation velocities are lower than the velocity of the rising fluid. The terminal velocity of any particle in any medium can be calculated using Stokes' Law if the particle's Reynolds number is below 0.2. 13. Elutriation
  • 15. Extraction in chemistry is a separation process consisting in the separation of a substance from a matrix. It includes Liquid-liquid extraction, and Solid phase extraction. 14. Extraction
  • 16. Field flow fractionation bbreviated FFF, is a separation technique where a field is applied to a fluid suspension or solution pumped through a long and narrow channel, perpendicular to the direction of flow, to cause separation of the particles present in the fluid, depending on their differing "mobilities" under the force 15. Field flow fractionation
  • 17. Froth flotation is a process for selectively separating hydrophobic materials from hydrophilic. This is used in mineral processing, paper recycling and waste-water treatment industries. Historically this was first used in the mining industry, where it was one of the great enabling technologies of the 20th century. It has been 16. Froth flotation
  • 18. Flocculation is a process wherein colloids come out of suspension in the form of floc or flake; either spontaneously or due to the addition of a clarifying agent. The action differs from precipitation in that, prior to flocculation, colloids are merely suspended in a liquid and not actually dissolved in a solution. In the flocculated system, there is no formation of a cake, since all the flocs are in the suspension. 17. Flocculation
  • 19. Fractional freezing is a misnomer, since it is not distillation but rather a process of enriching a solution by partially freezing it and removing frozen material that is poorer in the dissolved material than is the liquid portion left behind. Such enrichment parallels enrichment by true distillation, where the 18. Fractional freezing
  • 20. API oil–water separator is a device designed to separate gross amounts of oil and suspended solids from the wastewater effluents of oil refineries, petrochemical plants, chemical plants, natural gas processing plants and other industrial oily water sources. 19. API oil–water separator
  • 21. Magnetic separation is a process in which magnetically susceptible material is extracted from a mixture using a magnetic force. This separation technique can be useful in mining iron as it is attracted to a magnet. In mines where wolframite was mixed with cassiterite, such as South Crofty and East Pool mine in Cornwall or with bismuth such as at the Shepherd and Murphy mine in Moina, Tasmania, magnetic separation was used to separate the ores. 20. Magnetic separation
  • 22. Precipitation is the creation of a solid from a solution. When the reaction occurs in a liquid solution, the solid formed is called the 'precipitate'. The chemical that causes the solid to form is called the 'precipitant'. Without sufficient force of gravity (settling) to bring the solid particles together, the precipitate 21. Precipitation
  • 23. Recrystallization is a technique used to purify chemicals. By dissolving both impurities and a compound in an appropriate solvent, either the desired compound or impurities can be coaxed out of solution, leaving the other behind. It is named for the crystals often formed when the compound precipitates out. Alternatively, recrystallization can refer to the natural growth of larger ice crystals at 22. Recrystallization
  • 24. Scrubber systems are a diverse group of air pollution control devices that can be used to remove some particulates and/or gases from industrial exhaust streams. The first air scrubber was designed to remove carbon dioxide from the air of an early submarine, the Ictineo I, a role for which they continue to be used to this day 23. Scrubber
  • 25. Sedimentation is the tendency for particles in suspension to settle out of the fluid in which they are entrained and come to rest against a barrier. This is due to their motion through the fluid in response to the forces acting on them: these forces can be due to gravity, centrifugal acceleration, or electromagnetism. In geology, sedimentation is often used as the 24. Sedimentation
  • 26. Sieve A sieve, or sifter, is a device for separating wanted elements from unwanted material or for characterizing the particle size distribution of a sample, typically using a woven screen such as a mesh or net or metal.[1] The word "sift" derives from "sieve". In cooking, a sifter is used to separate and break up clumps in dry ingredients such as flour, as well as to aerate and combine them. A strainer is a form of sieve used to separate solids from liquid. 25. Sieve
  • 27. Stripping is a physical separation process where one or more components are removed from a liquid stream by a vapor stream. In industrial applications the liquid and vapor streams can have co-current or countercurrent flows. Stripping is usually carried out in either a packed or trayed column. 26. Stripping
  • 28. Sublimation is the transition of a substance directly from the solid to the gas phase without passing through the intermediate liquid phase. 27. Sublimation
  • 29. Vapor–liquid separator is a vertical vessel into which a liquid and vapor mixture (or a flashing liquid) is fed and wherein the liquid is separated by gravity, falls to the bottom of the vessel, and is withdrawn. The vapor travels upward at a design velocity which minimizes the entrainment 28. vapor–liquid separator
  • 30. Winnowing is an agricultural method developed by ancient cultures for separating grain from chaff. It is also used to remove weevils or other pests from stored grain. Threshing, the loosening of grain or seeds from the husks and straw, is the step in the chaff- removal process that comes before winnowing. 29. Winnowing
  • 31. Zone melting is a group of similar methods of purifying crystals, in which a narrow region of a crystal is melted, and this molten zone is moved along the crystal. The molten region melts impure solid at its forward edge and leaves a wake of purer material solidified behind it as it moves through the ingot. The impurities concentrate in the melt, and are moved to one end of the ingot. 30. Zone melting