This study aimed to clarify the mechanism of Sal B, a compound found in willow bark, in drug-resistant gastric cancer cells. Methods used included cell clone formation, flow cytometry to measure reactive oxygen species levels, TUNEL staining to visualize apoptotic cells, and western blot to identify AKT and mTOR proteins involved in apoptosis. Results showed that Sal B and the chemotherapy drug DDP promoted apoptosis in gastric cancer cells. The combination of Sal B and DDP further induced apoptosis in drug-resistant cells. Sal B was found to decrease levels of phosphorylated AKT and mTOR proteins, which regulate proliferation and apoptosis. The study suggests that Sal B enhances the effects of chemotherapy by promoting apoptosis via inhibition of the AKT

1. Facultad de Medicina
UPB Medellín
Presentado por:
Lorenzo Neira Perez & Steven Ortiz Toro
III Semestre

2. INTRODUCTION
1
2
Gastric cancer or stomach
cancer
Uncontrolled growth of cells
(tumor) that seek to invade
other areas of the body , and
there is no apoptosis
Depending on the location of the
tumor, it could give different
symptoms and have different
consequences.

3. INTRODUCTION
EMT:Epithelial–mesenchymal transition
characterized by the dissolution or attenuation
of epithelial adhesion, epithelial cells transform
into mesenchymal cells.
AKT - mTOR : the AKT-mTOR pathway regulates
proliferation, migration, cell survival,
metabolism, and apoptosis. when this pathway
is not regulated, cell proliferation increase

4. The study aims to clarify the
mechanism of Sal B in drug
resitant in gastric cancer cells.
OBJECTIVE

5. METHODS
Formación
de Clones
Consiste en la extracción de células de
un animal o planta y su posterior
crecimiento en un entorno artificial
favorable.
Se proporciona un sistema
modelo que sirve para evaluar:
fisiología, medicamentos,
mutaciones, compuestos tóxicos
y carcinogénesis en las células.

6. Es un método que permite la medición
rápida de algunas características físicas y
químicas de las células.
Se utilizo para cuantificar los niveles de
ROS intracelular.
Citometría de flujo
METHODS

7. METHODS
tinción tunnel
es un metodo que permite
la visualización y
cuatificación de céulas
apoptóticas .
Se basa en la degradación
de ADN y la tinción es
proporcional a las células
que mueren por apoptosis

8. Western Blot
METHODS
Es una técnica del laboratorio
usada para detectar una proteína
especifica utilizando un marcador
reactivo o químico, se hace para
identificar las prteinas AKT y
m/TOR en la apoptosis

9. C) Sal-B or DDP markedly promoted cell apoptosis in AGS cells.
D)The combination of Sal-B and DDP markedly induced cell
apoptosis in AGS/DDP cells. Data were shown as mean ± SD.
*p0.05, **p 0.01, ***p 0.001
RESULTS
FIG.3

10. FIG.4

11. A) Sal-B significantly decreased p-
AKT and p mTOR levels in AGS cells
and AGS/DDP cells
FIG.5
B) mTOR activator, MHY1485, or AKT
activator, SC79, significantly
upregulated AKT/mTOR signals in AGS
cells treated by Sal-B.

12. C) MHY1485 or SC79 markedly
promoted the phosphorylation of
AKT/ mTOR in AGS/DDP cells. Data
were shown as mean ± SD. *p 0.05,
**p 0.01, ***p 0.001
FIG.5

13. DISCUSSION
.Sal B regulates cell apoptosis via
AKT signals
AUTHOR ARGUMENT AGREE/
DISAGREE
.Sal B enhances the sensitivities of
cancer cells to chemotherapy and
radiotherapy
.EMT has been considered as one of
important mechanism in tumor
invasión and metastasis
.
Jing et al 2016 ; Yu
et al 2019
Guoet al 2018 ;
Wang et al 2013
Thiery 2002

14. CONCLUSIONS
Molecular biology allows you to study and see th relationship between some
proteins with certain diseases.
with the help of biology and laboratory test is possible to demostrate how is the
mechanism of action of certain medications and what other types of treatment
can work to cure or improve the patients life.
Molecular biology integrates various laboratory analithical methods that
facilitates the study of pathologies, medictions and cellular behavior.
Sal B and DDP could be a posible treatment for gastric cancer thanks to the
promotion of cell apoptosis

15. Lorenzo Neira

16. Steven Ortiz