This document summarizes work to construct an infectious clone of the squirrel monkey strain of simian foamy virus (SFVsqu). The researchers amplified fragments of the SFVsqu genome using PCR and plan to fuse the fragments together using Gibson assembly to generate a full-length circular viral genome. This cloned genome can then be transfected into mammalian cells to produce virus particles and allow study of the viral lifecycle. The researchers acknowledge funding sources and cite relevant references. Construction of this infectious clone will enable future study of how the human immune system responds to infection with New World Monkey strains of SFV.