Presentación del artículo previamente escogido, donde se habla de la búsqueda experimental de una vacuna para combatir el virus de la hepatitis C.

1. Susana Escobar Escobar Medicine Student III semester 2019-02

2. Introdution.
2
Flaviviridae family
Transmitted by contact with
infected blood;
Chronic hepatitis C
Infects approximately 70 million
people in the world
Acute hepatitis CT
Y
P
E
S

3. Introdution.
3
E2 protein - neutralizing
antibodies (NAbs)
Ferritin nanoparticle–
displaying system
sE2 experimental vaccines
High diversity of HCV
genomes

4. Objectives.
This study seeks to improve the
immunogenicity of sE2 by using ferritin
nanoparticles in order to find a vaccine for
the Hepatitis C virus.

5. 5
Electron microscopy. Materials and
methods.
Fundaments.What for?
To pick 10 981 particles of sE2-
ferritin or ferritin.
To generate 2-dimensional
classifications
Electron Microscopes are scientific
instruments that use a
beam of highly energetic electrons
to examine objects
on a very fine scale

6. 6
Materials and
methods.
Antibody Titer Assay and Neutralization
Assay
What for?
• Determine the conformation of
sE2
• Detect the target protein
Fundaments
Detects the presence and measures
the amount of antibodies within a
person’s blood.
The amount and diversity of
antibodies correlates to the strength
of the body’s immune response.

7. 7
Materials and
methods.
What for?
Animal Immunization
Determine the immunogenicity of
ferritin sE2
Interaction between the antigen and
the immune system.
If it is a viral antigen, the antigen will be
bound with MHC I protein and presented by
the antigen-presenting cell to a CD8 cell
which will likely trigger cell-mediated
immunity.
Fundaments

8. 8
Materials and
methods.
Receptor- and Antibody-Binding Assay.
What for?
Determine the conformation of sE2
displayed on the surface of ferritin
Fundaments
Measure interactions between two
molecules, to measure the affinity of
the reactants for each other.
Receptor

9. 9
Results.

10. 10
Results.

11. 11Results.

12. 12
Discussion.
Author Concept Yes or No
Wang X, Yan Y,
Gan T
Collectively, our results indicate that the sE2-
ferritin nanoparticle is an improved HCV
vaccine candidate
Falson P, Bartosch B,
Alsaleh K
It is thus likely that E2 might also be present
in a trimeric form on HCV virions
Reddy ST, Swartz MA,
Hubbell JA.
sE2- ferritin nanoparticles could more
efficiently activate immune system than the
sE2 subunit vaccine, because nanoparticles
can be readily captured and processed by
antigen-presenting cells (APCs), thus
facilitating the maturation of APCs and the
ac- tivation of the immune response

13. “
13
Conclusions
“We believe that these 2 strategies should be
combined to develop a nanoparticle-based
multivalent HCV vaccine with optimal
potency”.
sE2 and sE2-ferritin used as
nanoparticles could actually
represent the best way of treatment
for HCV

14. 14