Rory Eutsey has over 10 years of experience as a laboratory manager and senior microbiologist researching pathogens like Streptococcus pneumoniae and Haemophilus influenzae, with expertise in genomics, molecular biology techniques, and biofilm analysis; he has supervised many students and published numerous papers characterizing bacterial strains, virulence factors, and developing anti-biofilm strategies. Eutsey received his Master's and Bachelor's degrees in microbiology from the University of Georgia and Shippensburg University of Pennsylvania, respectively.

1. Rory Eutsey
913 Pleasant Valley Rd. Mt. Pleasant, PA 15666
Phone: 724-309-0858 Email: roryeutsey@gmail.com
Experience____________________________________
Laboratory Manager Aug 2008 – Present
Center for Genomic Sciences / Center for Excellence in Biofilm Research,
Allegheny Health Network, Allegheny Singer Research Institute
Allegheny General Hospital, Pittsburgh, PA
Research Fellow Dec 2006 – Aug 2008
Center for Genomic Sciences, Allegheny Singer Research Institute
Allegheny General Hospital, Pittsburgh, PA
Research Areas:
• Characterization of a novel restriction modification system in S. pneumoniae
• Study of an ocular pathogen lineage of S. pneumoniae
• Development of novel anti-biofilm strategies
• Comparative genomics and molecular pathogenesis of H. influenzae and
S. pneumoniae
• Biofilm associated infectious diseases, specifically otitis media and orthopedic
infections
• Quorum sensing in H. influenzae and S. pneumoniae
Primary Scientific duties:
• Strain engineering and characterization
• Protein purification and characterization
• Animal model studies
• Biofilm development and analysis
• Gene expression analysis
• Whole genome sequencing and genic arraying
Primary Managerial duties:
• Maintenance and repair of laboratory equipment
• Supervision of graduate students and interns
• Coordinating with collaborators and vendors
• Coordinating with building facilities personnel
• Maintaining stocks of lab supplies and reagents
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2. Eutsey, Senior Microbiologist
Laboratory Skills:
• Bacteriology: culture, isolation, transformation, biofilm development,
maintenance of stocks
• Cloning: construct design (Vector NTI), plasmid and linear fragment
assembly (restriction digest, ligation, Gibson assembly)
• DNA: extraction, quantification (Nanodrop, Agilent), purification, PCR, gel
electrophoresis
• RNA: extraction, quantification, cDNA synthesis, RT-PCR, qPCR,
Nanostring, microarray
• Sequencing: Sanger (ABI 3730), Next Generation (PacBio, Ion Torrent)
• Protein: tagging, purification, quantification, western blot
• Imaging: Xenogen bioluminescence in vivo imaging, fluorescent confocal
microscopy
• Animal: anesthesia, inoculation, observation, dissection, recovery of bacteria,
extraction of DNA from tissues.
Publications___________________________________________
• Eutsey R, Powell E, DordelJ, Salter SJ, Clark TA, Korlach J, Ehrlich GD, Hiller
NL. Genetic stabilization of the drug resistant PMEN1 pneumococcus lineage by
its distinctive DpnIII restriction-modification system. (Submitted for publication)
• Kadam A, Janto B, Eutsey R, Earl JP, Powell E, Dahlgren ME, Hu FZ, Ehrlich
GD, Hiller NL. Streptococcus pneumoniae Supragenome Hybridization Arrays for
Profiling of Genetic Content and Gene Expression. Curr Protoc Microbiol. 2015
Feb 2;36:9D.4.1-9D.4.20
• Long KB, Li Z, Burgwin CM, Choe SG, Martyanov V, Sassi-Gaha S, Earl JP,
Eutsey RA, Ahmed A, Ehrlich GD, Artlett CM, Whitfield ML, Blankenhorn EP.
The Tsk2/+ Mouse Fibrotic Phenotype Is Due to a Gain-of-Function Mutation in
the PIIINP Segment of the Col3a1 Gene. J Invest Dermatol. 2015 Mar;135(3):718-
27.
• Janto BA, Hiller NL, Eutsey RA, Dahlgren ME, Earl JP, Powell E, Ahmed A, Hu
FZ, Ehrlich GD. Development and validation of an Haemophilus influenza
supragenome hybridization (SGH) array for transcriptomic analyses. PLoS One.
2014 Oct 7;9(10):e105493.
• Eutsey RA, Hiller NL, Earl JP, Janto BA, Dahlgren ME, Ahmed A, Powell E,
Schultz MP, Gilsdorf JR, Zhang L, Smith A, Murphy TF, Sethi S, Shen K, Post JC,
Hu FZ, Ehrlich GD. Design and validation of a supragenome array for
determination of the genomic content of Haemophilus influenzae isolates. BMC
Genomics. 2013 Jul 17;14:484.
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3. Eutsey, Senior Microbiologist
• Hu FZ, Eutsey R, Ahmed A, Frazao N, Powell E, Hiller NL, Hillman T, Buchinsky
FJ, Boissy R, Janto B, Kress-Bennett J, Longwell M, Ezzo S, Post JC, Nesin M,
Tomasz A, Ehrlich GD. In vivo capsular switch in Streptococcus pneumoniae--
analysis by whole genome sequencing. PLoS One. 2012;7(11):e47983.
• Ahmed A, Earl J, Retchless A, Hillier SL, Rabe LK, Cherpes TL, Powell E, Janto
B, Eutsey R, Hiller NL, Boissy R, Dahlgren ME, Hall BG, Costerton JW, Post JC,
Hu FZ, Ehrlich GD. Comparative genomic analyses of 17 clinical isolates of
Gardnerella vaginalis provide evidence of multiple genetically isolated clades
consistent with subspeciation into genovars. J Bacteriol. 2012 Aug;194(15):3922-
37.
• Hiller NL, Eutsey RA, Powell E, Earl JP, Janto B, Martin DP, Dawid S, Ahmed A,
Longwell MJ, Dahlgren ME, Ezzo S, Tettelin H, Daugherty SC, Mitchell TJ,
Hillman TA, Buchinsky FJ, Tomasz A, de Lencastre H, Sá-Leão R, Post JC, Hu
FZ, Ehrlich GD. Differences in genotype and virulence among four multidrug-
resistant Streptococcus pneumoniae isolates belonging to the PMEN1 clone. PLoS
One. 2011;6(12):e28850.
• Hiller NL, Ahmed A, Powell E, Martin DP, Eutsey R, Earl J, Janto B, Boissy RJ,
Hogg J, Barbadora K, Sampath R, Lonergan S, Post JC, Hu FZ, Ehrlich GD.
Generation of genic diversity among Streptococcus pneumoniae strains via
horizontal gene transfer during a chronic polyclonal pediatric infection. PLoS
Pathog. 2010 Sep 16;6(9):e1001108.
• Eutsey R, Wang G, Maier RJ. Role of a MutY DNA glycosylase in combating
oxidative DNA damage in Helicobacter pylori. DNA Repair (Amst). 2007 Jan
4;6(1):19-26. Epub 2006 Sep 25.
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4. Eutsey, Senior Microbiologist
Education_____________________________________________
Master of Science – Microbiology Aug 2006
The University of Georgia, Athens, GA
Thesis project: Role of a MutY DNA glycosylase in combating oxidative DNA damage
in Helicobacter pylori
Principle investigator: Dr. Robert Maier
Bachelor of Science – Biology Dec 2003
Shippensburg University of Pennsylvania, Shippensburg, PA
NSF Research Experience for Undergraduates (REU) Participant Jun – Aug 2003
Department of Microbiology
The University of Georgia, Athens, GA
Advisor: Dr. William B. Whitman
Project Title: Isolation of novel microorganisms from no till agricultural soil
Supervising and Teaching Experience_____________________
Supervisor/Mentor for Technicians, Students and Interns Dec 2006 – Present
Center for Genomic Sciences, Allegheny Singer Research Institute
Allegheny General Hospital, Pittsburgh, PA
Mentor for Undergraduate Student Jan – May 2006
Department of Microbiology
The University of Georgia, Athens, GA
Graduate Teaching Assistant Aug – Dec 2005
Department of Microbiology
The University of Georgia, Athens, GA
Professor: Dr. Wendy Dustman
Class: MIBO 3510L, Introduction to Microbiology
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