RNA samples are extracted from leaf tissue and root tissue of a plant. Both samples are treated
with reverse transcriptase and the resulting cDNA are labelled with different fluorescent
compounds (leaf RNA = green, root RNA=red). The labelled cDNA are used to probe a
microarray carrying gene segments for a multitude of genes. The results of this experiment are in
the table below. The signal level indicates the amount of labelled cDNA of a particular color that
has hybridized to that gene spot. Which of the following statements are correct regarding the
interpretation of this data? Which of the following statements are correct regarding the
interpretation of this data? Tubulin is expressed at equal levels in the cells of the leaf and the
root. Genes with a green/red ratio above 1.0 are transcribed at a higher rate in the root tissue. The
gene coding for the pollen coat protein is not transcribed in either tissue. Asparagine synthase is
expressed at a higher rate in the cells of the leaf. Genes with a green/red ratio above 1.0 are
transcribed at a higher rate in leaf tissue. Expression of cytochrome oxidase is only important in
the cells of the leaf.
Hotspot mutation and fusion transcript detection from the same non-small cell...Thermo Fisher Scientific
The presence of certain chromosomal Header
rearrangements and the subsequent fusion
gene derived from translocations has been
implicated in a number of cancers. Hundreds of
translocations have been described in the
literature recently but the need to efficiently
detect and further characterize these
chromosomal translocations is growing
exponentially. The two main methods to identify
and monitor translocations, fluorescent in situ
hybridization (FISH) and comparative genomic
hybridization (CGH) are challenging, labor
intensive, the information obtained is limited,
and sensitivity is rather low. Common sample
types for these analyses are biopsies or small
tumors, which are very limited in material
making the downstream measurement of more
than one analyte rather difficult; obtaining
another biopsy, using a different section or
splitting the sample can raise issues of tumor
heterogeneity. The ability to study mutation
status as well as measuring fusion transcript
expression from the same sample is powerful
because you’re maximizing the information
obtained from a single precious sample and
eliminating any sample to sample variation.
Here we describe the efficient isolation of two
valuable analytes, RNA and DNA, from the
same starting sample without splitting, followed
by versatile and informative downstream
analysis. This methodology has been applied to
FFPE and degraded samples as well as fresh
tissues, cells and blood. DNA and RNA were
recovered from the same non-small cell lung
adenocarcinoma sample and both mutation
analysis, as well as fusion transcript detection
was performed using the Ion Torrent PGM™
platform on the same Ion 318™ chip. Using
10ng of DNA and 10ng of RNA input, we
applied the Ion AmpliSeq™ Colon and Lung
Cancer panel to analyze over 500 COSMIC
mutations in 22 genes and the Ion AmpliSeq™
RNA Lung Fusion panel to detect 40 different
fusion transcripts.
RNAi is a powerful, conserved biological process through which the small, double-stranded RNAs specifically silence the expression of homologous genes, largely through degradation of their cognate mRNA.
It highlights the various methods of gene transfer in plants, characterization of plants by PCR and qRTPCR. Different types of PCR and Real time PCR have been described
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...OECD Environment
10-12 April 2019: The OECD Conference on RNAi based pesticides provided an overview on the current status and future possibilities for the regulation of externally applied dsRNA-based products that are proposed for use as pesticides. The event facilitated exchanges between policy makers, academia, industry on their implications in health, environment, and regulation.
Question 6 3 pts There is evidence that tornados occur with increased.pdfgazender686
Question 3:
The management of Unter Corporation, an architectural design firm, is considering an
investment with the following cash flows:
Required:
1. Determine the payback period of the investment.
2. Would the payback period be affected if the cash inflow in the last year were several times as
large?
Year Investment Cash Inflow
1 $ 76,000 $ 5,000
2 $ 3,000 $ 10,000
3 $ 18,000
4 $ 19,000
5 $ 22,000
6 $ 20,000
7 $ 18,000
8 $ 16,000
9 $ 15,000
10 $ 15,000
Required: 1. Determine the payback period of the investment. 2. Would the payback period be
affected if the cash inflow in the last year were several times as large? Complete this question by
entering your answers in the tabs below. Determine the payback period of the investment.
(Round your answer to 1 decimal place.) Would the payback period be affected if the cash
inflow in the last year were several times as large?.
Question 3 Not yet answered Points out of 1-00 P Flag question For the.pdfgazender686
Question 2. A repairman moves between 4 sites, with site 1 denoting home office, and 2,3 , and 4
denoting remote sites. Work at sites 2,3, and 4 requires the use of an equipment trailer. The cost
of relocating the equipment trailer between the sites is d ( j , k ) = 300 for k = j . The cost c ( k , j
) of using the trailer is 100 if the work is at site k > 1 and trailer is at site j = k with j > 1 ; 50 if j
= k and j > 1 , and 200 if the work is at k > 1 and the trailer is at j = 1 (home office). If the
repairman is at the home office, no work is done and cost is zero. At any time, the repairman
knows his present job location, observes the location of the next job (or 1 if no job is to be done)
and decides whether to move the trailer. The transition matrix between job locations is P = 0.1
0.0 0.0 0.4 0.3 0.5 0.0 0.0 0.3 0.5 0.8 0.0 0.3 0.0 0.2 0.6 For example, if the current location (job)
is 2, the probability that the next job is at site 3 is 0.5 . Assume the discount factor of 0.95 .
(Q2.1) Write the dynamic programming equations for the optimal value function. (Q2.2) Solve
these equations by the value iteration method (starting from 0 ) and calculate the lower and upper
bounds at each step. (Q2.3) Solve the problem by the policy iteration method starting from "do
dot move trailer from base" at each state. (Q2.4) Solve the problem by linear programming..
More Related Content
Similar to RNA samples are extracted from leaf tissue and root tissue of a plant-.pdf
Hotspot mutation and fusion transcript detection from the same non-small cell...Thermo Fisher Scientific
The presence of certain chromosomal Header
rearrangements and the subsequent fusion
gene derived from translocations has been
implicated in a number of cancers. Hundreds of
translocations have been described in the
literature recently but the need to efficiently
detect and further characterize these
chromosomal translocations is growing
exponentially. The two main methods to identify
and monitor translocations, fluorescent in situ
hybridization (FISH) and comparative genomic
hybridization (CGH) are challenging, labor
intensive, the information obtained is limited,
and sensitivity is rather low. Common sample
types for these analyses are biopsies or small
tumors, which are very limited in material
making the downstream measurement of more
than one analyte rather difficult; obtaining
another biopsy, using a different section or
splitting the sample can raise issues of tumor
heterogeneity. The ability to study mutation
status as well as measuring fusion transcript
expression from the same sample is powerful
because you’re maximizing the information
obtained from a single precious sample and
eliminating any sample to sample variation.
Here we describe the efficient isolation of two
valuable analytes, RNA and DNA, from the
same starting sample without splitting, followed
by versatile and informative downstream
analysis. This methodology has been applied to
FFPE and degraded samples as well as fresh
tissues, cells and blood. DNA and RNA were
recovered from the same non-small cell lung
adenocarcinoma sample and both mutation
analysis, as well as fusion transcript detection
was performed using the Ion Torrent PGM™
platform on the same Ion 318™ chip. Using
10ng of DNA and 10ng of RNA input, we
applied the Ion AmpliSeq™ Colon and Lung
Cancer panel to analyze over 500 COSMIC
mutations in 22 genes and the Ion AmpliSeq™
RNA Lung Fusion panel to detect 40 different
fusion transcripts.
RNAi is a powerful, conserved biological process through which the small, double-stranded RNAs specifically silence the expression of homologous genes, largely through degradation of their cognate mRNA.
It highlights the various methods of gene transfer in plants, characterization of plants by PCR and qRTPCR. Different types of PCR and Real time PCR have been described
Validation of RNA interference by RNA-Seq: How to see the big picture - Brend...OECD Environment
10-12 April 2019: The OECD Conference on RNAi based pesticides provided an overview on the current status and future possibilities for the regulation of externally applied dsRNA-based products that are proposed for use as pesticides. The event facilitated exchanges between policy makers, academia, industry on their implications in health, environment, and regulation.
Question 6 3 pts There is evidence that tornados occur with increased.pdfgazender686
Question 3:
The management of Unter Corporation, an architectural design firm, is considering an
investment with the following cash flows:
Required:
1. Determine the payback period of the investment.
2. Would the payback period be affected if the cash inflow in the last year were several times as
large?
Year Investment Cash Inflow
1 $ 76,000 $ 5,000
2 $ 3,000 $ 10,000
3 $ 18,000
4 $ 19,000
5 $ 22,000
6 $ 20,000
7 $ 18,000
8 $ 16,000
9 $ 15,000
10 $ 15,000
Required: 1. Determine the payback period of the investment. 2. Would the payback period be
affected if the cash inflow in the last year were several times as large? Complete this question by
entering your answers in the tabs below. Determine the payback period of the investment.
(Round your answer to 1 decimal place.) Would the payback period be affected if the cash
inflow in the last year were several times as large?.
Question 3 Not yet answered Points out of 1-00 P Flag question For the.pdfgazender686
Question 2. A repairman moves between 4 sites, with site 1 denoting home office, and 2,3 , and 4
denoting remote sites. Work at sites 2,3, and 4 requires the use of an equipment trailer. The cost
of relocating the equipment trailer between the sites is d ( j , k ) = 300 for k = j . The cost c ( k , j
) of using the trailer is 100 if the work is at site k > 1 and trailer is at site j = k with j > 1 ; 50 if j
= k and j > 1 , and 200 if the work is at k > 1 and the trailer is at j = 1 (home office). If the
repairman is at the home office, no work is done and cost is zero. At any time, the repairman
knows his present job location, observes the location of the next job (or 1 if no job is to be done)
and decides whether to move the trailer. The transition matrix between job locations is P = 0.1
0.0 0.0 0.4 0.3 0.5 0.0 0.0 0.3 0.5 0.8 0.0 0.3 0.0 0.2 0.6 For example, if the current location (job)
is 2, the probability that the next job is at site 3 is 0.5 . Assume the discount factor of 0.95 .
(Q2.1) Write the dynamic programming equations for the optimal value function. (Q2.2) Solve
these equations by the value iteration method (starting from 0 ) and calculate the lower and upper
bounds at each step. (Q2.3) Solve the problem by the policy iteration method starting from "do
dot move trailer from base" at each state. (Q2.4) Solve the problem by linear programming..
Question 2-2 Make a list of four groups of the people who may be poten.pdfgazender686
Question 2: (a) It is known that 56% of the students prefer having longer library hours. In a
recent survey of 750 students, 400 students expressed an interest in having longer library hours.
What is the probability that less than 400 students prefer to have longer library hours? Question 2
(Continued): (b) What is the probability that in a survey of 750 students, the number of students
wanting longer library hours is between 400 and 450 students, inclusive?.
Question 5 (5 points) A downturn in the market has caused severe finan.pdfgazender686
Question 29 Which of the following correctly describes desert streams? Desert streams have
long, frequent tributaries. Desert streams have decreasing discharge downstream. Desert streams
have short, frequent tributaries. Desert streams have increasing discharge downstream. Desert
streams have well-sorted, rounded clasts of sediment..
Question 41 (2 points) What does it mean to say that HSV-1 is often la.pdfgazender686
QUESTION 23 How do hypotheses differ from theories? Theories are well-tested and supported
by data Theories are more specific than hypotheses. Hypotheses are educated guesses, while
theories are based on observation. Predictions can be made based on theories, but not based on
hypotheses..
Question 4- What factors effect the level of payment from both public.pdfgazender686
Question 22 (1 point)
A random sample of 81 credit sales in a department store showed an average sale of $68.00.
From past data, it is known that the standard deviation of the population is $27.00. At 0.95
confidence interval, what is the margin of error?
Question 22 options:
a)
4.89
b)
5.88
c)
3.00
d)
4.50
e)
None of the options
Question 23 (1 point)
A random sample of 49 lunch customers was taken at a restaurant. The average amount of time
these customers stayed in the restaurant was 45 minutes with a standard deviation of 14 minutes.
With a 95% probability, what is the size of the margin of error?
Question 23 options:
a)
3.2
b)
2.901
c)
None of the options
d)
2
e)
4.022
Question 24 (1 point)
A random sample of 49 lunch customers was taken at a restaurant. The average amount of time
these customers stayed in the restaurant was 45 minutes with a standard deviation of 14 minutes.
Construct a 95% confidence interval for the true average amount of time customers spent in the
restaurant.
Question 24 options:
a)
43 to 47 minutes
b)
44.70 to 52.02 minutes
c)
42.88 to 46.22 minutes
d)
None of the options
e)
40.98 to 49.02 minutes
Question 25 (1 point)
The average monthly electric bill of a random sample of 256 residents of a city is $111 with a
standard deviation of $32. Construct a 90% confidence interval for the mean monthly electric
bills of all residents.
Question 25 options:
a)
$109.28 to $116.71
b)
None of the options
c)
$112.10 to $116.98
d)
$107.71 to $114.29
e)
$107.08 to $114.92
Question 26 (1 point)
A sample of 225 elements from a population with a standard deviation of 75 is selected. The
sample mean is 180. The 98% confidence interval for is ______________
Question 26 options:
a)
None of the options
b)
175 to 185
c)
105 to 225
d)
165.6 to 194.4
e)
164.58 to 195.43
Question 27 (1 point)
A new brand of chocolate bar is being market tested. A sample of 500 of the new chocolate bars
were given to consumers to try. The consumers were asked whether they liked or disliked the
chocolate bar. The responses indicated that 200 disliked the chocolate bar. What is the point
estimate for the proportion of people who disliked the chocolate bar?
Question 27 options:
a)
0.20
b)
0.60
c)
0.80
d)
0.40
e)
None of the options
Question 28 (1 point)
A new brand of chocolate bar is being market tested. A sample of 500 of the new chocolate bars
were given to consumers to try. The consumers were asked whether they liked or disliked the
chocolate bar. The responses indicated that 400 liked the chocolate bar. What is the point
estimate for the proportion of people who liked the chocolate bar?
Question 28 options:
a)
0.75
b)
0.80
c)
0.20
d)
0.25
e)
None of the options
please answer within an hour
a) 4.89
b) 5.88
c) 3.00
d) 4.50
e) None of the options.
QUESTION 2 Suppose an individual has a utility function given by U(W).pdfgazender686
Question 2 of 2 Question 2. Add parametersarguments to a function. - R. Revise the code below:
so that if accept names as an argument. b. Call the function and test if it wocks as intended.
Example output froea your function: when you call the fusction using the name 'Fatmar, the
function shoold ourpus: Hello Fatma Hew do you do Fatuna?.
Question 2 The following data set includes the volcanos in the world-.pdfgazender686
Question 2 The Fibonacci numbers are defined as a) Using the programming language of your
choice, write a program to compute the Fibonacci number n and perform empirical measurement
to record the time to compute the Fibonacci for different input sizes (i.e. different value of n ). b)
Perform theoretical analysis to find the time complexity of the Fibonacci function. c) Discuss the
correlation between your empirical analysis result and the theoretical analysis of the Fibonacci
function..
Question 2 The Fibonacci numbers are defined as a) Using the programmi (1).pdfgazender686
Question 2
The basic idea of a regression is very simple. We have some X values (we called these BLANK and some Y value
(this is the variable we are trying to BLANK. We could have multiple Y values, but that is not something we have
covered.
First blank answers (re-ordered ordinals, non-numerical inputs, numeric inputs, features, intercepts)
Second blank answers (order, predict, change, advance)
Question 5
What is a good model fit value?
O R-squared of .4
O R-squared of p-value minus .05
O Unknowable without knowing/understanding the context and the domain.
O 95% accurate
O 99% accurate.
O R-squared of .95
O R-squared of .8
Question 9
The features in a model....
None of these answers are correct
Keep the model validation process stable
Are used as proxies for y-hat/y (that is yhat divided by y)
Are always functions of each other.
Question 4 Presentation Layer and MVC Compare and contrast Java presen.pdfgazender686
Question 2. You are examining a population of a plant species. You noticed that 30% of
individuals have orange flowers and the remaining have white flowers. If the allele that codes for
orange flowers is recessive, what is the frequency of heterozygotes in this population?
a. The information is insufficient to estimate the number of heterozygotes.
b. 0.49
c. 0.30
d. 0.70.
Question 4 The number of days of absence from school for those juvenil (1).pdfgazender686
Question 2.3
Write a out a mission statement for your business
What does it mean to be an entrepreneur? The road to entrepreneurship is often a treacherous one
filled with unexpected detours, roadblocks and dead ends. There are lots of sleepless nights,
plans that do not work out, funding that does not come through and customers that never
materialise. It can be so challenging to launch a business that it makes one wonder why anyone
willingly sets out on such a path. Despite all of these hardships, every year, thousands of
entrepreneurs embark on this journey determined to bring their vision to fruition and fill a need
they see in society. They open brick-and-morlar businesses, launch tech startups or bring a new
product or service into the marketplace. An entrepreneur identifies a need that no existing
businesses addresses and determines a solution for that need. Entrepreneurial activity includes
developing and launching new businesses and marketing them, often with the end goal of selling
the business to turn a profit. An entrepreneur who regularly launches new businesses, sells them
and then starts new businesses is a serial entrepreneur. Additionally, although the term
"entrepreneur" is often associated with startups and small businesses, any founder of a successful
housenold-name business began as an entrepreneur. If you want to become an entrepreneur but
worried that you do not have the necessary finances, it should not stop you from achieving your
goals. Many entrepreneurs seek the initial funding for their pursuits from external sources, who
may provide entrepreneurs with capital to cover start-up costs (or, later, expansion costs.) Many
household-name businesspersons exemplify entrepreneurial success. Here are just a few
examples: 1. Steve Jobs, the late tech leader who started Apple in a garage and grew it to the
dominant tablet, smartphone and Computer Company it is today. 2. Elon Musk, who founded
SpaceX and has since become known for putting the billions of dollars his company has earned
him toward projects, including providing clean water to Flint, Michigan, and donating FDA-
approved ventilators to hospitals fighting COVID-19. 3. Bill Gates, the Microsoft creator and
founder who has often been listed as the world's wealthiest individual and has become a global
leader on pandemics and how to handle them. The question most people ask is - what motivates
entrepreneurs to venture forth when so many others would run in the opposite direction?
Although each person's motivation is nuanced and unique, many entrepreneurs are spurred on by
one or more of the following motivators: 1. Autonomy: Entrepreneurs want to be their own
bosses, set their own goals, control their own progress and run their businesses how they see fit.
They recognise that their business's success or failure rests with them, yet they do not view this
responsibility as a burden but, instead, as a marker of their freedom. 2. Purpose: Many
entrepreneurs have a clear vis.
Question 4 Homwwork - Uninswerad Compare the generation time of B- sub.pdfgazender686
Question 2: Three approaches to achieving competitive advantage for a business are cost
leadership, differentiation, and focus. Is it possible for a community general hospital to use both
a cost leadership and differentiation method with separate services in the hospital? Explain your
response.
Question 3: A variety of tools is available for analyzing a portfolio of strategic business units.
Which of these tools would you suggest health services managers use when attempting to gain
insight into the aptitudes and skills required for the manager of a soon-to-be-developed business
unit? Explain your selection..
QUESTION 4 How could you declare a vector that is storing Person point.pdfgazender686
Question 2: What are the causes of poverty? Are they structural or individual? How is one's
answer to this question likely to affect one's view of the justice or injustice of poverty?
Question 3: What moral Obligation, if any, do we have individually and as a society to reduce
poverty? What steps could be taken what role should business play?.
Salvador is well-liked by his team members- He is always agreeable to.pdfgazender686
Salvador is well-liked by his team members. He is always agreeable to new ideas and listens to
what his team members have to say. This is an example of which leader characteristic?
multicultural beliefs
pro-diversity beliefs
cognitive complexity
leader personality factors
a. multicultural beliefs
b. pro-diversity beliefs
c. cognitive complexity
d. leader personality factors.
Salmonella enterica is a gastrointestinal pathogen whereas most strain.pdfgazender686
Salmonella enterica is a gastrointestinal pathogen whereas most strains of Escherichia coli and
Klebsiella are generally non-pathogenic, yet all three may found in one sample. On the basis of
the MR/VP and SIM results, is it possible to differentiate Salmonella from Escherichia or
Klebsiella? If so, which test(s) would be most useful for this purpose.
Sally the Forsterite Stealer loves forsterite- When a bulk composition.pdfgazender686
Sally the Forsterite Stealer loves forsterite. When a bulk composition consisting of 70% Fo, 10%
An, and 20% Qz begins crystallizing, she awaits hungrily. As soon as the liquid composition hits
the An-En tie line, she swoops in and steals all the forsterite. Trace the resulting paths of liquid
and bulk solids. How would the paths differ if Sally instead stole every last crystal of forsterite?
Phase diagram to draw on:.
Sales for the year were $159-000- The balance sheet at the end of the.pdfgazender686
Sales for the year were $159 , 000 . The balance sheet at the end of the year is given below: Part
1 What is the fixed asset turnover ratio? What is the fixed asset turnover ratio? Part 2 What is the
total asset turnover ratio?.
Saina- a registered medical assistant- has just taken Sheldon Armstron.pdfgazender686
Saina, a registered medical assistant, has just taken Sheldon Armstrong to an examination room
and obtained his chief complaint. She notes his chief complaint as follows: Patient states "I have
been having intense stomach pain. What other information should Saina obtain from the patient
regarding his chief complaint?.
Riverbed Corp lost most of its inventory in a fire in December fust be.pdfgazender686
Riverbed Corp lost most of its inventory in a fire in December fust before the year end physicali
impentogy was tiken. The Riveried Cotpiakinne tooks disclosed the following? Merchandise
with a selling price of $42 , 000 remalned undamaged after the fire. Damaged merchandise with
an of jiral seiling price of $28 , 000 had a net realizable value of $9 , 600 ..
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Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
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This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
RNA samples are extracted from leaf tissue and root tissue of a plant-.pdf
1. RNA samples are extracted from leaf tissue and root tissue of a plant. Both samples are treated
with reverse transcriptase and the resulting cDNA are labelled with different fluorescent
compounds (leaf RNA = green, root RNA=red). The labelled cDNA are used to probe a
microarray carrying gene segments for a multitude of genes. The results of this experiment are in
the table below. The signal level indicates the amount of labelled cDNA of a particular color that
has hybridized to that gene spot. Which of the following statements are correct regarding the
interpretation of this data? Which of the following statements are correct regarding the
interpretation of this data? Tubulin is expressed at equal levels in the cells of the leaf and the
root. Genes with a green/red ratio above 1.0 are transcribed at a higher rate in the root tissue. The
gene coding for the pollen coat protein is not transcribed in either tissue. Asparagine synthase is
expressed at a higher rate in the cells of the leaf. Genes with a green/red ratio above 1.0 are
transcribed at a higher rate in leaf tissue. Expression of cytochrome oxidase is only important in
the cells of the leaf
