Budin Ghyslain is a French national born in 1983 with experience in organic chemistry, chemical biology, biophysics, and cancer biology. He holds a PhD in organic synthesis from Strasbourg University and has held postdoctoral positions at EPFL and Harvard Medical School developing probes for fluorescence imaging. Currently he is a senior scientist and project manager at Intrace Medical SA developing new in vitro and in vivo assays.

1. Budin Ghyslain, PhD
PERSONNAL DETAILS
Birth date: 1983-11-09
Nationality: French
Address: 87b Avenue de Bâle, 68300 Saint-Louis (FR)
Personal tel: (+41) 079 952 06 52
E-mail: budin.ghyslain@gmail.com
Suisse G work Permit
I am a highly motivated individual with an excellent academic education together with an
interdisciplinary mindset, outstanding commitment and a wide range of experience in chemical biology,
including synthetic organic chemistry, biophysics, imaging and cancer biology. I value multi-disciplinary
work and enjoy devising and implementing innovative solutions in a challenging and dynamic
atmosphere. My qualities include inquisitiveness, interpersonal skills, and leadership skills. I am
conscientious and dedicated to continuing personal and professional development.
PROFESSIONAL EXPERIENCES
2013/11 Senior scientist - Project manager at Intrace Medical SA, Lausanne, Switzerland, 50%
Current Develop and lead new projects, validation of new in vitro/in vivo assays and protocols.
Communication and link between the Employer and EPFL. Intellectual property filing
and tracking. Organization of sales. External communication in conferences and
collaborations.
2013/06 Consultant for Intrace Medical SA, Lausanne, Switzerland
2013/11 Written and oral consultation concerning Company’s activities in the field of
bioluminescent probe and functionalized near infrared fluorescent dyes and other
scientific questions. Consulting and answering the questions of Company’s customers.
Company’s external communication in international congress.
2012/11 Postdoctoral position, Laboratory of Bioorganic chemistry and Molecular Imaging
Current Supervisor: Prof. E. Dubikovskaya, EPFL, Lausanne, Switzerland, now at 50%
General project: Development of chemical tools for fluorescence image-guided
surgery, in vivo bioluminescence and PET imaging
Drive and coordinate projects within the group, generate strategic input for probe
design. Interaction with external collaborators. Close supervision of multiple PhD and
masters students, guide and motivate people. Grants, patents, animal protocol and
publication writing.
2010/10 Postdoctoral position, Center for Systems Biology
2012/10 Supervisor: Prof. R. Weissleder, MGH, Harvard Medical School, Boston, USA
Research project: Development of imaging agent for in vivo detection of cancer targets
Development of new bioorthogonal probes for in vivo imaging of cancer proliferation
markers, (Polo-Like Kinase 1, Aurora kinase A, VEGFR2, BCl2). Synthesis of probes
for detection, quantification and identification of bacteria (Gram positive/Gram
negative, mycobacterium tuberculosis). Development of tools for identifying clinical
drug’s specificity.
2006/09 Ph.D. in organic synthesis, Laboratory of Functional Chemo-System
2010/06 Supervisors: Dr. C.Mioskowski and Dr. A. Wagner, Strasbourg University, France
Research project: Molecular toolbox synthesis for functional biology and proteomics
Synthesis of new molecular tools for protein profiling in living cells. Fluorescent
chemical labeling of protein in non-denaturing conditions. Synthesis of fluorescent
probes engaged in bioorthogonal coupling by click-chemistry or Staudinger ligation.

2. EDUCATION
2006/2010 Ph.D. in organic chemistry, Strasbourg University, France
2004/2006 Master of Science in chemistry (M.Sc.), Strasbourg University, France, Degree ranked
top of year
2001-2004 Bachelor of Science in chemistry (B.Sc.), Aix-Marseille III University, France, Degree
ranked top of year
SCHOLARSHIPS AND PRIZES
2005 Ministry of research and education prize for excellent academic achievements
2006 PhD fellowship from the French ministry for excellent academic achievements
2009 Young investigator award, HUPO 8th
2010 Best poster award, RICT 46th
2012 Poster distinction award, SAC 65th
TEACHING AND FORMATION
Teaching: Thermodynamic 3rd year Licence (2006/2007), organic chemistry 1st year
Master (2007/2009), general chemistry 1st year License (2006/2009)
Formation: CIES (Centre Initiation enseignement supérieur) teaching formation
Mouse-handling: Module 1 animal training (Switzerland)
PERSONAL SKILLS
Synthetic Organic and Inorganic Chemistry
Multistep organic synthesis, peptidic coupling, microwave-assisted reaction,
methodology. Bioorthogonal reactions, catalytic reactions, protecting group strategies
Instrumentation: NMR (1H, 13C, DEPT, COSY, NOESY, 31P, 19F), IR, UV, fluorescence.
Highly experience with U/HPLC (Analytical and preparative), MS. Flash
chromatography, GC, Isothermal Titration Calorimeter.
Biology, Biochemistry and imaging
Cell culture, Electrophoresis gel, fluorescence gel scanning, DNA gel separation,
western blot, PCR, transfection, proteins pull-down, immunoprecipitation, mass
spectrometry for proteins identification (nanoLC/MS/MS), antibody drug conjugate.
FACS, fluorescent confocal microscopy (Deltavision API, Olympus FV1000, Zeiss
LSM700) intravital microscopy. in vivo imaging on mouse model: bioluminescence,
fluorescence imaging, IVIS (FLIT, DLIT, DYCE), Computed Tomography, image
reconstruction, ex vivo analysis. Understanding of a broad range of in vitro and in vivo
assays.
Competences
Team work, leadership spirit, organizational and management skills, capacity of
communication, dynamic. Grants, patents and research proposals writing. Project
report and oral presentation. Publication writing. Animal protocol writing.
Project design and management, interdisciplinary project leader. Undergraduates and
PhDs supervision and training.
COMPUTING AND LANGUAGES
Windows, MacOS
Word, Excel, PowerPoint, keynote, pages, Chemdraw, Beilstein, Scifinder, reaxys,
prism, Pymol, Fiji, MestreNova, Living image.
French: mother tongue
English: fluent (read, written and spoken), TOEIC 845/990 (2009/03/10)
Spanish: good knowledge
German: Beginner (actually learning)

3. PUBLICATIONS AND CONGRESS
Publications 1-Azidation of β-carbonyl lactones and lactams, D. Kashinath, G. Budin, R. Baati, S.
Meunier, and A. Wagner, Tetrahedron Letter, 2009, 50, 5379-5381
2-Molecular tool-box for in vivo functional biology and proteomics: Application to DNA
topoisomerase II, G. Budin, M. Moune, L. brino, A. Wagner, The journal of
translational Molecular Medecine, 2009, 5, S1, 175 (abstract paper from the HUPO
8th congress)
3-A Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions, G.
Budin, M. Moune-Dimala, V. Lamour, P. Oudet, C. Mioskowski, S. Meunier, L. Brino A.
Wagner, ChemBioChem, 2010, 11, 79-82
4-Optimization of the azobenzene scaffold for reductive cleavage by dithionite;
development of a novel azobenzene cleavable linker for proteomic applications, G.
Leriche, G. Budin, L. Brino, A. Wagner, Eur J.Org. Chem., 2010, 4360–4364
5-Non-denaturing chemical proteomics for protein complex isolation and identification,
G. Budin, M. Moune-Dimala, G. Leriche, J.M. Saliou, J. Papillon, S. Sanglier-Cianferani,
A. Van Dorsselaer, V. Lamour, L. Brino, A. Wagner, ChemBioChem, 2010, 11, 2359-
2361
6-Tailoring drug release profile of low-molecular-weight hydrogels by supramolecular
co-assembly and thiol-ene orthogonal coupling, D. Díaz Díaz, E. Morin, E.M. Schön, G.
Budin, A. Wagner, J-S. Remy, J. Mater. Chem., 2011, 21, 641-644
7-FRET-Based probe with a chemically deactivatable quencher, G. Leriche, G. Budin,
A. Weiss, L. Brino, A. klymchenko, Y. Mély, A. Wagner, Chem. Commun. 2012, 48,
3224-3226
8-Bioorthogonal Probes for Polo-Like Kinase 1 Imaging and Quantification, G. Budin,
K.S. Yang, T. Reiner, R. Weissleder, Angew. Chem. Int. Ed., 2011, 50 (40), 9378-
9381
9-Ubiquitous Detection of Gram-Positive Bacteria with Bioorthogonal
Magnetofluorescent Nanoparticles, H.J. Chung, T. Reiner, G. Budin, C. Min, M. Liong,
D. Isadore, H. Lee, R. Weissleder, ACS Nano. 2011, 5, 8834-8841
10-Bioorthogonal imaging of aurora kinase A in live cells, G. budin, K.S. Yang, T.
Reiner, C. Vinegoni, R. Weissleder, Angew. Chem. Int. Ed. 2012, 51, 6598-6603
11-A magnetic Gram stain for bacteria detection, G. Budin, H.J. Chung, H. Lee, R.
Weissleder, Angew. Int. Chem. Ed, 2012, 51 (31) 7752-7755. Selected as ‘Hot paper’
12-Magnetic labeling bacteria, G. Budin, R. Weissleder, H. Lee, H.J. Chung, Patent
issue 2012/03/08, WO 2013187954 A1
13-μHall Chip for Sensitive Detection of Bacteria, D. Issadore, H.J. Chung, J. Chung,
G. Budin, R. Weissleder, H. Lee, Adv Healthc Mater. 2013, 2, 1224-1228.
14-Bioorthogonal approach to identify unsuspected drug targets in live cells, G. Budin,
K.S. Yang, C. Tassa, O. Kister, R. Weissleder, Angew. Chem. Int. Ed. 2013, 52,
10593 –10597. Selected as ‘Hot paper’
15-A Biocompatible "Split Luciferin" Reaction and its Application for Non-Invasive
Bioluminescent Imaging of Protease Activity in Living Animals, A. Godinat, G. Budin, A.
Morales, H.M. Park, L.E. Sanman, M. Bogyo, A. Yu, A. Stahl, E.A. Dubikovskaya,
Current protocols 2014, 6 (3), 169-89.
16-Preparation of bioluminescence imaging small biomolecules, S. Riccardo, E.
Dubikovskaya, G. Budin, G. Karateev, J. Frigell, A. Konovalova, A. Godinat, PCT Int.
Appl. 2014, WO 2014111906.

4. 17- 6 provisional patents (US-PPA-N.62/006,440); (US-PPA-N.62/006,406); (US-PPA-N.
61/892,633); (US-PPA-N.61/754,765); (US-PPA-N.61/754,750); (19357/US)
Congress SECO 46th Semaine d’Etude de Chimie Organique. La Rochelle (France) 2009/05
HUPO 8th International congress of Human Proteome Organization. Toronto (Canada)
2009/09
RICT 46th International Conference on medicinal chemistry, Reims (France) 2010/06.
ISCM International Symposium Congress on Medicinal chemistry, Brussel (Belgium)
2010/09
Center for Systems Biology retreat, Boston (USA) 2011/06
Annual meeting of the MGH Scientific Advisory Comity SAC 65th Boston (USA) 2012/04
Revolutionaries for Global Health Summit (Perkin Elmer), Brussel (Belgium) 2013/03
EMIM 8th European Molecular Imaging Meeting, Torino, (IT) 2013/05
WMIC 6th World Molecular Imaging Congress, Savannah (USA) 2013/09
ESMI 9th European Molecular Imaging Meeting, Antwerp, (BE) 2014/06
INTEREST AND ACTIVITIES
Sport: football, volleyball, snowboard
Culture: cinema, museum, music, literature (Bernard Werber, Dan Brown).
REFERENCES
Alain Wagner, Ph.D. Research Director, Laboratory of Functional Chemo-System (LFCS), Illkirch, FR
wagner@bioorga.u-strasbg.fr, tel.(33) 3 68 85 42 97
Valérie Lamour, Ph.D. Research Director, Laboratory of Biochemistry and Molecular Biology, Strasbourg, FR
lamourv@igbmc.fr, tel.(33) 3 88 65 32 36
Ralph Weissleder, M.D., Ph.D., Center for Systems Biology, MGH, Harvard Medical School, Boston, USA
sdlaft@mgh.harvard.edu, tel.(1) 617-726-7870