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pg. 1 Judith Luber-Narod, Ph. D.
JUDITH A. LUBER-NAROD, Ph. D.
email: jlnarod@gmail.com
Summary
 Experienced cellular/molecular pharmacologist/ in vitro toxicologist with expertise in Neuroimmunology,
Autoimmunity, Oncology and Engineering.
 Excellent written and oral communication skills.
Education and Licensure
B. S. Biology, State University of New York at Stony Brook
Ph. D. Pharmacology, University of Miami School of Medicine, Miami, Florida
Advanced Course, “Pathogenesis of Neuroimmunologic Diseases”, Marine Biological Institute, Woods Hole,
Massachusetts.
Employment History
Industrial Scientist:
Senior Scientist/ Department Head, In Vitro Toxicology, PharmaMar USA, Inc., Cambridge, Massachusetts.
Responsibilities included assay development and validation, data analysis and software acquisition, laboratory
administration, QC, establishment of protocols and procedures, equipment purchases, supervision of lab personnel,
presentations at national and international conferences, preparation and presentation of reports and publications. Reported
to the Toxicology Division Leader. Major accomplisments: Developed, organized, managed new laboratory at early start-
up phase of company. Developed in vitro assays using novel cell line panel to screen for NCE toxicity. Developed
methods for investigating drug-drug interactions in vitro. Applied immunohistochemical and enzymatic fluorescence
techniques to investigating mechanisms and targets of in vitro toxicity. Served on management team involved in all phases
of drug development--part of PharmaMar’s first successful product, Yondelis®, from pre-clinical investigations to market,
as well as the development of 6 other candidates through various stages. Signatory on 3 PharmaMar patents in the area of
Neuroblastoma.
Visiting Scientist, Massachusetts Institute of Technology/Northeastern University, Artificial Liver Engineering. Winner,
1st
Place, AAAS- ERN Conference, Teacher division, Washington, D. C. for this research. Laboratory of Carol Livermore.
Academic Scientist/Professor:
Adjunct Professor of Surgery/Urology, University of Massachusetts Medical Center. Adjunct Professor of Biology
and Biotechnology, Worcester State College.
Assistant Professor of Surgery/Urology, Immunology and Diabetes and Endocrinology Research Center, University
of Massachusetts Medical Center. Investigated the role of neuropeptides in the inflammation characteristic of Interstitial
Cystitis, using both rat and human tissues as models. Developed autoimmune animal models of Interstitial Cystitis (IC)
and Nonbacterial Prostatitis for use in investigating their pathogenesis/etiology; listed in the NIH Animal Model registry.
Major accomplisments: First to identify autoantibodies and cytokines responsible for the elevated urinary frequency
observed in Experimental Autoimmune Cystitis. Identified changes in the glycosaminoglycan layer of the bladder which
may be related to IC pathogenesis. Investigated the role of sex hormones in the animal models and in in vitro culture
systems. First to show an autoimmune etiology to both IC and Prostatitis. Research was fully funded by an NIH RO1, PI
Judith Luber-Narod.
Instructor, Department of Surgery/Urology, University of Massachusetts Medical Center. Initial investigation of the
role of neuropeptides in the inflammation characteristic of Interstitial Cystitis. Also investigated the toxic and proliferative
effects of oxalate as a potential causative agent of kidney stones.
Instructor, Department of Cellular and Molecular Physiology, University of Massachusetts Medical Center.
Advisors: Susan E. Leeman, Robert Carraway. Determined that cultured neuroglial cells secrete lymphokines in
response to substance P in vitro; a mechanism which may play a role in the neuropathology seen in Multiple Sclerosis,
Alzheimer's Disease, and AIDS-related dementia, etc. Proved that the NK1 (substance P) receptor is coupled to a
heterotrimeric G-protein. Also showed that the neurotensin precursor protein is phosphorylated effecting its processing
into peptides. Major accomplisments: first to identify classical cytokine mechanisms in the CNS immune system.
pg. 2 Judith Luber-Narod, Ph. D.
Postdoctoral fellow, Department of Neurology, University of Massachusetts Medical Center. Advisor: Joseph
Rogers. 1st to identify HLA-DR and other immune antigens in the human central nervous system and to correlate this
expression with aging and Alzheimer's disease pathology (30 years before the identification of lymphatic vessels in the
brain!). Developed sensitive methods for detection of immunologic antigens on human brain tissue sections still used
today to validate mouse models of Alzheimer’s Disease.
Postdoctoral fellow, Department of Physiology, University of Miami School of Medicine. Advisor: Robert Keene.
Developed purified populations of glial cells in culture--Oligodendrocytes, Astrocytes, and Microglia, and distinguished
them using immunocytochemical means. Investigated the lineage of these cell populations and their phagocytic
capabilities.
Postdoctoral fellow, Department of Pharmacology, University of Miami School of Medicine. Advisor: Lincoln T.
Potter. Investigated kinetic properties of ligand binding to G-protein coupled muscarinic receptors.
Predoctoral fellow, Department of Pharmacology, University of Miami School of Medicine. Advisor: Lincoln T.
Potter. Dissertation: Two Populations of Muscarine Receptors, M1 and M2. First to identify two populations of
Muscarinic receptors, M1 and M2. Developed receptor binding strategies for detecting and quantitating two muscarinic
receptor subtypes and a pharmacologic screening method for determining the drug selectivity for the subtypes. Identified
the signal transduction mechanism of M2, i.e. coupling to a heterotrimeric G-protein.
High School Teacher:
High School Science Teacher, Nipmuc High School, Upton MA. Teach 110 students, Biology and Ecology and
Populations.
 Presented Best Practices to school officials and teachers, Writing Culturally Sensitive Tests.
High School Science Teacher, Brockton High School, Brockton, MA.
Teach Honors Chemistry and College Preparatory Biology to Sophomores taking the MCAS examinations.Wrote the
curriculum for our half-year Chemistry course. Started Brockton High School’s first Academic Quiz Team and took them
to compete at the PBS High School Quiz Show competition.
http://www.enterprisenews.com/article/20141107/NEWS/141107163
High School Science Teacher, Abby Kelley Foster Charter School, Worcester, MA.
Designed, wrote and taught curriculum in Honors Biology and College Preparatory Biology, “Humans and the
Environment”, Environmental Earth Science and Character Development program. Also taught: International
Baccalaureate (IB), Honors and College Preparatory (CP) Chemistry and Environmental Sciences (International Pe
Environmental Systems and Societies, Environmental Earth Science (CP).National Honor Society Advisor, Environmental
Action Club founder and advisor. Started school-wide recycling program, Regional (MA) Winner, Advisor to school
Academic Quiz team
Participant in the RET (Research Experiences for Teachers) Program, Department of Engineering, Northeastern
University and Boston University. Presented research at Massachusetts Association of Science Teachers meeting,.
Laboratories of Shasty Murthy (Microfluidics, NEU), Ferdi Hellweger (Environmental EngineeringNEU), and Darren
Roblyer (Photonics, BU),
Substitute teacher, all subjects, multiple districts in Massachusetts, including Shrewsbury, Northboro, Framingham,
Watertown, and Hudson Schools.
Community Service:
 Organizer of “Be a Fairy Godmother” campaign to find prom dresses for students who can’t afford to purchase
them, Rock On Power Off Environmental Concert, School recycling program, Abby Kelley Foster Charter
School.
 Kids Campaign to Purchase a Fire Truck, Worcester County, MA.
 Boroughs’ Katrina Relief Fund, Westboro, Northboro, Southborough, MA.
 Vice President, board member and committee chair, Beth Tikvah Synagogue, Westboro, MA. Volunteer for
Rachel's Table, Worcester, MA.,
 Visiting Scientist Program, University of Massachusetts Medical School, Mentor- Howard Hughes Summer
Teaching Fellows and Fellowship Program, University of Massachusetts Medical School
 Host family, PAX Spain Exchange Student program, Israeli Student Representatives.
pg. 3 Judith Luber-Narod, Ph. D.
Honors, awards, professional activities
 Recipient of Boehringer Ingelheim Research Award, Muscarinic Receptors
 Member New York Academy of Sciences
 Certificate of Recognition, Interstitial Cystitis Association.
 Recipient, Green Dependence Day Award, sponsored by National Grid and the Alliance for Climate Education.
 One of 2 teachers nationally selected for film by the Alliance for Climate Education, www.youtube.com/
 Trailblazing Teacher Award, The Center for Green Schools http://centerforgreenschools.org/
 New England Aquarium’s Ocean Stewardship Educator of the Year. http://news.neaq.org.
 Green Classroom Professional certificate, National Center for Green Schools.
 2013 Award, from the House of Representatives of the State of Massachusetts, for Commitment to
Environmental Education.
 Green Classroom Professional exam writer, National Center for Green Schools.
 1st
place ERN Conference, Washington D.C. (see below for abstract).
Specialized Skills
 Managerial and Communication skills: Grant writing/management, Laboratory management, Personnel
management, Mediation Counseling, Budgeting, QC, project management, SOP/Protocol/Report writing/editing,
teaching, excellent verbal and written presentation skills.
 Computer Skills: Data analysis (dose response relationships, drug combination studies, etc), statistical analyses,
curve fitting applications, graphics applications, presentation software, digital image acquisition and analysis,
spreadsheets. Write my own scripts where needed for some applications (e.g. Prism, Excel). Integrated several
packages for complex scientific analyses. (Familiar with CalcuSyn, Prism, Softmax, OpenLab, Photoshop,
SigmaPlot, Microsoft Office, to name a few.).
 Tissue and Cell Culture: Primary CNS culture, primary culture of hematopoetic cells, culture of various cell
lines, flow cytometry, cytotoxicity and viability/apoptosis assays, proliferation assays, secretion studies,
enzymatic assays, cell based ELISAs, phagocytosis assays, drug combination/interaction studies.
 Immunologic techniques: Autoimmune animal models (Experimental autoimmune cystitis, encephalomyelitis,
and prostatitis), ELISA assays, T-cell purification, culture, and adoptive transfer, Western blot analyses,
immunoprecipitation, cytokine assays, immunohistochemistry, immunocytochemistry, histochemistry, flow
cytometry.
 Animal models: Experimental Autoimmune Cystitis (1st to develop this model), Estrogen induced Autoimmune
Prostatitis, (1st to show the autoimmune nature of this condition), Experimental Autoimmune Encephalomyelitis,
secondary models of all three via lymphocyte adoptive transfer, oncologic models.
 Microscopy and pathology: fluorescence microscopy, phase microscopy, histochemical staining and
interpretation of basic pathological specimens, imaging, microphotography.
 Receptor and Signal Transduction Biochemistry: Receptor binding assays, receptor kinetics, guanine
nucleotide binding assays (G proteins), receptor autoradiography.
 Protein Biochemistry: Protein purification, column chromatography, HPLC, gel electrophoresis, Western Blot
analyses, phosphorylation assays, enzyme kinetics, in vitro assays.
 Urologic and related animal techniques: Urinary frequency assays (1st to develop this assay), bladder capacity
assays, bladder histology, urinary catheterization (both male and female rats), animal surgery.
 Molecular biology: RNA isolation, PCR, Northern Blot analyses, restriction enzyme digest analyses.
 Scientific writing: grant writing, paper writing, able to bring science to a level comprehensible by non-scientists.
 Teaching: Graduate Level Biology, Pharmacology, High School Biology, Chemistry, Environmental Sciences,
IB, Honors, College Preparatory and Basic levels. Curriculum writing, laboratory design, student and club
advisory, IB, STEM, use of technology in the classroom
pg. 4 Judith Luber-Narod, Ph. D.
Publications
1. J. Luber-Narod, B. Smith, W. Grant, J. M. Jimeno, L. López-Lázaro, and G. T. Faircloth.(2001). Evaluation of
the use of in vitro methodologies as a tool for screening new compounds for in vivo toxicity potential. Tox. In
Vitro 15:571-577.
2. Mitra, S. P., Carraway, R. E., Blute, R., Jr., and Luber-Narod, J. (1999). Agonist induced conformation alteration
of neurotensin receptor and the mechanism behind Na+ inhibition of 125I-NT binding. J. Receptor Signal
Transduction Res., 19:995-1021.
3. Mitra, S., Dagher, A., Kage, R., Dagher, R. K. and Luber-Narod, J. (1999). Experimental Autoimmune Cystitis:
Further characterization and serum antibodies Urol. Res. 27:351-356.
4. Luber-Narod, J., Austin-Ritchie, T., Hollins, C., III, Menon, M., Maholtra, R., Baker, S. and R.E. Carraway.
(1997). Role of substance P in several models of bladder inflammation. Urol. Res. 25:395-399.
5. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M. (1996). Experimental
Inflammatory Cystitis in the Lewis Rat a potential animal model for Interstitial cystitis. Urol. Res. 24:367-373.
6. Laxmanan, S., Bala, R.S., Malhotra, R.K., Miller-Graziano, C., Menon, M. and Luber-Narod, J. (1996). Estrogen
induced Autoimmune Prostatitis in the Rat. J. Urol. 156:1838-1840.
7. Scheid, C. R., Koul, H., Hill, W. A., Luber-Narod, J., Jonassen, J., Honeyman, T., Kennington, L., Kholi, R.,
Hodapp, J., Ayvazian, P., and Menon, M. (1996). Oxalate Toxicity in LLC-PK1 cells, a line of renal epithelial
cells. J.Urol. 155:1112-1116..
8. Scheid, C.R., Koul, H., Kennington, L., Hill, W.A., Luber-Narod, J., Jonassen, J., Honeyman, T., and Menon, M.
(1996). Oxalate-induced Damage to Renal Tubular Cells. Scanning Microscopy International 9:1097-1107.
9. Scheid, C. Koul, H., Hill, W.A., Luber-Narod, J. Kennington, L., Honeyman, T., Jonassen, J. and Menon, M.
(1996). Oxalate toxicity in LLC-PK1 cells: role of free radicals. Kidney International, 49:413-419.
10. Luber-Narod, J. Kage, R., and S.E. Leeman. (1994). Substance P enhances the secretion of TNF alpha from
neuroglial cells stimulated with LPS. J. Immunol. 152:819-824.
11. Ferris, C.F., Delville, Y., Grzonka, Z., Luber-Narod, J., and Insell, T.R. (1993) An iodinated Vasopressin (V1)
antagonist blocks flank marking and selectively labels neural binding sites in Golden Hamsters. Physiology and
Behavior 54:737-747.
12. Boyd, N.D., MacDonald, S., Kage, R., Luber-Narod, J. and Leeman, S.E. (1991). Substance P receptor:
Characterization and G protein interactions. In Substance P and Related Peptides : Cellular and Molecular
Physiology., S.E. Leeman, J.E. Krause, and F. Lembeck, ed., N.Y. Academy of Sciences, 1991.
13. Oblas, B., Boyd, N.D., Luber-Narod, J. Reyes, V.E., and S.E. Leeman. (1990). Isolation and identification of a
polypeptide in the HSP family that binds substance P. Biochem. Biophys. Res. Comm. 166:978-983.
14. Luber-Narod, J., Boyd, N.D. and Leeman, Susan. (1990). Guanine nucleotides decrease the affinity of substance
P binding. European J. Pharmacol., Mol. Pharmacol Section, 188:1-7.
15. Rogers, J., Luber-Narod, J., Mufson, E.J., Styren, S. D., and Civin, W. H. (1988) Presence of immune system
markers in human brain and their potential role as a primary pathogenetic mechanism Alzheimer's Disease. In:
Molecular Biology of Alzheimer's Disease, Caleb E. Finch and Peter Davies, ed. Cold Spring Harbor
Laboratory Press.
16. Rogers, J., and J. Luber-Narod. (1988). Immune actions in the central nervous system: a brief review with special
emphasis on Alzheimer's Disease. Drug Develop. Res. 15:227-235.
17. 3. Rogers, J., Luber-Narod, J., Styren, S. D., and W.H. Civin. (1988). Expression of immune system associated
antigens by cells of the human central nervous system: relationship to the pathology of Alzheimer's Disease.
Neurobiology of Aging, 9:339-349.
18. Luber-Narod, J. and Rogers, J. (1988). Immune system associated antigens expressed by cells of the human
central nervous system. Neuroscience Letters, 94:17-22.
19. Potter, L.T., Flynn, D.D., Hanchett, H.E., Kalinoski, D.L., Luber-Narod, J., and Mash, D.C. (1984).Independent
M1 and M2 receptors: Ligands, Autoradiography, and Functions.In: Trends in Pharmacological Sciences,
Suppl.: 22-32.
pg. 5 Judith Luber-Narod, Ph. D.
Abstracts
1. Judith Luber-Narod, Ph. D., Brockton High School, Charlie Wheeler, Lauren Chai, Massachusetts Institute of
Technology (MIT), Phillipp J. Mehner, Majid Bigdeli Karimi, Northeastern University (NEU), Martin
Culpepper, Ph. D., MIT, and Carol Livermore, Ph. D., NEU. Engineering of a Transplantable Liver based on
Liver Biology and Origami-folded Materials. (2015), Proceedings of the ERN, AAAS Annual Meeting,
February, 2015.
2. Judy Luber-Narod, Jose Maria Fernandez Sousa-Faro, Carmen Cuevas, Pablo Aviles, Glynn T. Faircloth. (2005).
In vitro toxicology studies of Lamellarins M, D and K and 4 synthetic analogs. Proceedings AACR, 2005,
abstract #639.
3. Susana G. Gómez, Judith Luber-Narod, Glynn T. Faircloth, Juan A. Bueren and Beatriz Albella. (2002). In vitro
toxicity of kahalalide f, a new antineoplastic drug. Toxicology In Vitro, Proceedings 2nd international joint
meeting: in vitro models and toxicity mechanism.
4. Luber-Narod, J, Ubezio, P., D’Incalci, Maurizio, Grant, W., Smith B. and GT Faircloth.. The in vitro toxicity of
ET-743 is antagonized by co-administration with paclitaxel., Toxicology In Vitro, Proceedings 2nd international
joint meeting: in vitro models and toxicity mechanism., p. 44.
5. Luber-Narod, J., Smith, B., Grant, W., Jimeno, J., López-Lázaro, L., and GT. Faircloth (2001). In vitro safety
toxicology of Ecteinascidin 743, a marine natural product with chemotherapeutic potential against solid tumors,
Proceedings AACR 2001, p212.
6. Luber-Narod, J., Smith, B., Grant, W., Jimeno, J., López-Lázaro, L., and GT. Faircloth (2001). In vitro safety
profile of Aplidine, a marine natural product with chemotherapeutic potential., Proceedings AACR 2001, p70.
7. Ady-Vago, N., L López-Lázaro, S Faivre, C Lacroix, J Luber-Narod, D. Adams, L Paz-Ares, C Chiabrando, C
Guzman, N Celli, GT Faircloth, JP Armand, JM Fernandez-Sousa, JM Jimeno,,and E. Raymond. (2000). L-
carnitine as a protector against aplidin induced skeletal muscle toxicity. Proceedings AACR 2001, p. 544
8. Luber-Narod, J, Smith, B, Grant, W, Jimeno, J, López-Lázaro, L, Scotto, K, Shtil, A. and Faircloth G.T. (2000).
In vitro safety toxicology of Kahalalide F, a marine natural product with chemotherapeutic potential against solid
tumors. EORTC-AACR Clin. Canc. Res.
9. Luber-Narod, J. and Delville, Y. (1999). Ovariectomy decreases urinary frequency in an animal model. NE
section, American Urological Association Annual Meeting.
10. 24. Luber-Narod, J. and Delville, Y. (1999). Ovariectomy decreases urinary frequency in an animal model. Soc.
Neurosci. Abst.
11. Luber-Narod, J. (1999). Glycosaminoglycan changes in the urine of rats with experimental autoimmune cystitis
(EAC). J. Autoimmunity, Suppl. 1999, p.51.
12. Luber-Narod, J. (1999). J. Experimental autoimmune cystitis (EAC): Role of autoantibodies. J. Autoimmunity,
Suppl. 1999, p.106.
13. Luber-Narod, J. (1998). Experimental Autoimmune Cystitis: The Role Of Autoantibodies. Soc. For Neurosci
Abst. 24:1857.
14. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M.(1996) Experimental
Inflammatory Cystitis: An animal model of IC. J. Urol. 142.
15. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M.(1996) Experimental
Inflammatory Cystitis: An animal model of IC. J. Urol. 142.
16. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M.(1995) Experimental
Inflammatory Cystitis in the Lewis Rat characterized histologically and behaviorally. NIDDK/ Interstitial
Cystitis Association Annual Meeting on Interstitial Cystitis.
17. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M.(1995) Experimental
Autoimmune Cystitis in the Lewis Rat characterized histologically and behaviorally. New England AUA
Annual Meeting.
18. Luber-Narod, Austin-Ritchie, T., Price, H., Dagher, R., Menon, M. and R.E. Carraway. (1995). Role of
neuropeptides in the xylene induced model of bladder inflammation. NIDDK conference on Interstitial
Cystitis.
19. Scheid, C. Koul, H., Kennington, L., Hill, A., Luber-Narod, J., and Menon, M. (1994) Oxalate induced damage
in renal epithelial cells, a possible factor in kidney stone disease. FASEB abstracts 8:408.
20. Luber-Narod, J. Kage, R. and Leeman, S.E. (1994). Substance P stimulates the secretion of tumor necrosis factor
from rat brain glia in culture. IBRO abst.
21. MacDonald, S.G., Luber-Narod, J. and Boyd, N.D. (1990). Crosslinking of the substance P receptor to a 43 kDa
polypeptide: a potential G protein alpha subunit. Soc. Neurosci. Abst. 14:515.
22. Luber-Narod, J., Boyd, N.D., Oblas, B., and S.E. Leeman (1987). A GTP-insensitive component of high affinity
substance P binding to receptors from rat salivary gland. Soc. Neurosci. Abst. 13:412.
23. Boyd, N.D., Luber-Narod, J., Oblas, B., and S.E. Leeman. (1987). Reconstitution of high affinity substance P
binding by purified guanine nucleotide regulatory proteins. Soc. Neurosci. Abst. 13:412.
pg. 6 Judith Luber-Narod, Ph. D.
24. Rogers, J. , Styren, S. D., and Luber-Narod, J. (1987). Expression of immune system associated antigens by cells
of the human central nervous system . Soc. Neurosci. Abst.13:1328.
25. Rogers, J., Luber-Narod, J., and Styren, S.D. (1987). Expression of immune system markers in human brain and
its potential pathogenetic significance in Alzheimer's Disease. Satellite symposium on Autoimmunity and
Alzheimer's Disease, Society for Neurosciences.
26. Rogers. J., Singer, R.H., Luber-Narod, J., and Bassell, G. (1986). Neurovirologic and neuroimmunologic
considerations in Alzheimer's Disease. Soc. Neurosci. Abst. 12:944.
27. Luber-Narod, J. , Flynn, D.D., and Potter, L.T. (1984). Binding of selective agonists and antagonists to two
interconvertible states of M1 muscarine receptors. Soc. Neurosci. Abst. 10:568.
28. Potter, L.T. and Luber-Narod, J. (1983). Distribution and function of M1 and M2 muscarine receptors in
different tissues. Soc. Neurosci. Abst. 9:582.
29. Luber-Narod, J. and Potter, L.T. (1983) Selectivity of drugs for M1 and M2 muscarine receptors. Soc. Neurosci.
Abst. 9:582.
30. Potter, L.T., Luber-Narod, J., and Wohlberg, C.J. (1982). EDTA amplifies agonist- and antagonist-binding
phenomena, and helps resolve different types of receptors. Soc. Neurosci. Abst. 8:339.
31. Flynn, D.D., Kalinoski, D.L., Luber-Narod, J. and Potter, L. T. (1982). Evidence for guanine nucleotide-sensitive
regulatory protein with muscarine receptors in membranes and in solution. Soc. Neurosci. Abst. 8:339.
32. Luber-Narod, J. and Potter, L.T. (1982). Two types of muscarine receptors (M1 and M2) in membranes from the
rat forebrain and brainstem. Soc. Neurosci. Abst. 8:338.
33. Luber-Narod, J. and Potter, L.T. (1981). Three agonist and two antagonist states of muscarine receptors. Soc.
Neurosci. Abst. 7:498.

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jln cv may 2016

  • 1. pg. 1 Judith Luber-Narod, Ph. D. JUDITH A. LUBER-NAROD, Ph. D. email: jlnarod@gmail.com Summary  Experienced cellular/molecular pharmacologist/ in vitro toxicologist with expertise in Neuroimmunology, Autoimmunity, Oncology and Engineering.  Excellent written and oral communication skills. Education and Licensure B. S. Biology, State University of New York at Stony Brook Ph. D. Pharmacology, University of Miami School of Medicine, Miami, Florida Advanced Course, “Pathogenesis of Neuroimmunologic Diseases”, Marine Biological Institute, Woods Hole, Massachusetts. Employment History Industrial Scientist: Senior Scientist/ Department Head, In Vitro Toxicology, PharmaMar USA, Inc., Cambridge, Massachusetts. Responsibilities included assay development and validation, data analysis and software acquisition, laboratory administration, QC, establishment of protocols and procedures, equipment purchases, supervision of lab personnel, presentations at national and international conferences, preparation and presentation of reports and publications. Reported to the Toxicology Division Leader. Major accomplisments: Developed, organized, managed new laboratory at early start- up phase of company. Developed in vitro assays using novel cell line panel to screen for NCE toxicity. Developed methods for investigating drug-drug interactions in vitro. Applied immunohistochemical and enzymatic fluorescence techniques to investigating mechanisms and targets of in vitro toxicity. Served on management team involved in all phases of drug development--part of PharmaMar’s first successful product, Yondelis®, from pre-clinical investigations to market, as well as the development of 6 other candidates through various stages. Signatory on 3 PharmaMar patents in the area of Neuroblastoma. Visiting Scientist, Massachusetts Institute of Technology/Northeastern University, Artificial Liver Engineering. Winner, 1st Place, AAAS- ERN Conference, Teacher division, Washington, D. C. for this research. Laboratory of Carol Livermore. Academic Scientist/Professor: Adjunct Professor of Surgery/Urology, University of Massachusetts Medical Center. Adjunct Professor of Biology and Biotechnology, Worcester State College. Assistant Professor of Surgery/Urology, Immunology and Diabetes and Endocrinology Research Center, University of Massachusetts Medical Center. Investigated the role of neuropeptides in the inflammation characteristic of Interstitial Cystitis, using both rat and human tissues as models. Developed autoimmune animal models of Interstitial Cystitis (IC) and Nonbacterial Prostatitis for use in investigating their pathogenesis/etiology; listed in the NIH Animal Model registry. Major accomplisments: First to identify autoantibodies and cytokines responsible for the elevated urinary frequency observed in Experimental Autoimmune Cystitis. Identified changes in the glycosaminoglycan layer of the bladder which may be related to IC pathogenesis. Investigated the role of sex hormones in the animal models and in in vitro culture systems. First to show an autoimmune etiology to both IC and Prostatitis. Research was fully funded by an NIH RO1, PI Judith Luber-Narod. Instructor, Department of Surgery/Urology, University of Massachusetts Medical Center. Initial investigation of the role of neuropeptides in the inflammation characteristic of Interstitial Cystitis. Also investigated the toxic and proliferative effects of oxalate as a potential causative agent of kidney stones. Instructor, Department of Cellular and Molecular Physiology, University of Massachusetts Medical Center. Advisors: Susan E. Leeman, Robert Carraway. Determined that cultured neuroglial cells secrete lymphokines in response to substance P in vitro; a mechanism which may play a role in the neuropathology seen in Multiple Sclerosis, Alzheimer's Disease, and AIDS-related dementia, etc. Proved that the NK1 (substance P) receptor is coupled to a heterotrimeric G-protein. Also showed that the neurotensin precursor protein is phosphorylated effecting its processing into peptides. Major accomplisments: first to identify classical cytokine mechanisms in the CNS immune system.
  • 2. pg. 2 Judith Luber-Narod, Ph. D. Postdoctoral fellow, Department of Neurology, University of Massachusetts Medical Center. Advisor: Joseph Rogers. 1st to identify HLA-DR and other immune antigens in the human central nervous system and to correlate this expression with aging and Alzheimer's disease pathology (30 years before the identification of lymphatic vessels in the brain!). Developed sensitive methods for detection of immunologic antigens on human brain tissue sections still used today to validate mouse models of Alzheimer’s Disease. Postdoctoral fellow, Department of Physiology, University of Miami School of Medicine. Advisor: Robert Keene. Developed purified populations of glial cells in culture--Oligodendrocytes, Astrocytes, and Microglia, and distinguished them using immunocytochemical means. Investigated the lineage of these cell populations and their phagocytic capabilities. Postdoctoral fellow, Department of Pharmacology, University of Miami School of Medicine. Advisor: Lincoln T. Potter. Investigated kinetic properties of ligand binding to G-protein coupled muscarinic receptors. Predoctoral fellow, Department of Pharmacology, University of Miami School of Medicine. Advisor: Lincoln T. Potter. Dissertation: Two Populations of Muscarine Receptors, M1 and M2. First to identify two populations of Muscarinic receptors, M1 and M2. Developed receptor binding strategies for detecting and quantitating two muscarinic receptor subtypes and a pharmacologic screening method for determining the drug selectivity for the subtypes. Identified the signal transduction mechanism of M2, i.e. coupling to a heterotrimeric G-protein. High School Teacher: High School Science Teacher, Nipmuc High School, Upton MA. Teach 110 students, Biology and Ecology and Populations.  Presented Best Practices to school officials and teachers, Writing Culturally Sensitive Tests. High School Science Teacher, Brockton High School, Brockton, MA. Teach Honors Chemistry and College Preparatory Biology to Sophomores taking the MCAS examinations.Wrote the curriculum for our half-year Chemistry course. Started Brockton High School’s first Academic Quiz Team and took them to compete at the PBS High School Quiz Show competition. http://www.enterprisenews.com/article/20141107/NEWS/141107163 High School Science Teacher, Abby Kelley Foster Charter School, Worcester, MA. Designed, wrote and taught curriculum in Honors Biology and College Preparatory Biology, “Humans and the Environment”, Environmental Earth Science and Character Development program. Also taught: International Baccalaureate (IB), Honors and College Preparatory (CP) Chemistry and Environmental Sciences (International Pe Environmental Systems and Societies, Environmental Earth Science (CP).National Honor Society Advisor, Environmental Action Club founder and advisor. Started school-wide recycling program, Regional (MA) Winner, Advisor to school Academic Quiz team Participant in the RET (Research Experiences for Teachers) Program, Department of Engineering, Northeastern University and Boston University. Presented research at Massachusetts Association of Science Teachers meeting,. Laboratories of Shasty Murthy (Microfluidics, NEU), Ferdi Hellweger (Environmental EngineeringNEU), and Darren Roblyer (Photonics, BU), Substitute teacher, all subjects, multiple districts in Massachusetts, including Shrewsbury, Northboro, Framingham, Watertown, and Hudson Schools. Community Service:  Organizer of “Be a Fairy Godmother” campaign to find prom dresses for students who can’t afford to purchase them, Rock On Power Off Environmental Concert, School recycling program, Abby Kelley Foster Charter School.  Kids Campaign to Purchase a Fire Truck, Worcester County, MA.  Boroughs’ Katrina Relief Fund, Westboro, Northboro, Southborough, MA.  Vice President, board member and committee chair, Beth Tikvah Synagogue, Westboro, MA. Volunteer for Rachel's Table, Worcester, MA.,  Visiting Scientist Program, University of Massachusetts Medical School, Mentor- Howard Hughes Summer Teaching Fellows and Fellowship Program, University of Massachusetts Medical School  Host family, PAX Spain Exchange Student program, Israeli Student Representatives.
  • 3. pg. 3 Judith Luber-Narod, Ph. D. Honors, awards, professional activities  Recipient of Boehringer Ingelheim Research Award, Muscarinic Receptors  Member New York Academy of Sciences  Certificate of Recognition, Interstitial Cystitis Association.  Recipient, Green Dependence Day Award, sponsored by National Grid and the Alliance for Climate Education.  One of 2 teachers nationally selected for film by the Alliance for Climate Education, www.youtube.com/  Trailblazing Teacher Award, The Center for Green Schools http://centerforgreenschools.org/  New England Aquarium’s Ocean Stewardship Educator of the Year. http://news.neaq.org.  Green Classroom Professional certificate, National Center for Green Schools.  2013 Award, from the House of Representatives of the State of Massachusetts, for Commitment to Environmental Education.  Green Classroom Professional exam writer, National Center for Green Schools.  1st place ERN Conference, Washington D.C. (see below for abstract). Specialized Skills  Managerial and Communication skills: Grant writing/management, Laboratory management, Personnel management, Mediation Counseling, Budgeting, QC, project management, SOP/Protocol/Report writing/editing, teaching, excellent verbal and written presentation skills.  Computer Skills: Data analysis (dose response relationships, drug combination studies, etc), statistical analyses, curve fitting applications, graphics applications, presentation software, digital image acquisition and analysis, spreadsheets. Write my own scripts where needed for some applications (e.g. Prism, Excel). Integrated several packages for complex scientific analyses. (Familiar with CalcuSyn, Prism, Softmax, OpenLab, Photoshop, SigmaPlot, Microsoft Office, to name a few.).  Tissue and Cell Culture: Primary CNS culture, primary culture of hematopoetic cells, culture of various cell lines, flow cytometry, cytotoxicity and viability/apoptosis assays, proliferation assays, secretion studies, enzymatic assays, cell based ELISAs, phagocytosis assays, drug combination/interaction studies.  Immunologic techniques: Autoimmune animal models (Experimental autoimmune cystitis, encephalomyelitis, and prostatitis), ELISA assays, T-cell purification, culture, and adoptive transfer, Western blot analyses, immunoprecipitation, cytokine assays, immunohistochemistry, immunocytochemistry, histochemistry, flow cytometry.  Animal models: Experimental Autoimmune Cystitis (1st to develop this model), Estrogen induced Autoimmune Prostatitis, (1st to show the autoimmune nature of this condition), Experimental Autoimmune Encephalomyelitis, secondary models of all three via lymphocyte adoptive transfer, oncologic models.  Microscopy and pathology: fluorescence microscopy, phase microscopy, histochemical staining and interpretation of basic pathological specimens, imaging, microphotography.  Receptor and Signal Transduction Biochemistry: Receptor binding assays, receptor kinetics, guanine nucleotide binding assays (G proteins), receptor autoradiography.  Protein Biochemistry: Protein purification, column chromatography, HPLC, gel electrophoresis, Western Blot analyses, phosphorylation assays, enzyme kinetics, in vitro assays.  Urologic and related animal techniques: Urinary frequency assays (1st to develop this assay), bladder capacity assays, bladder histology, urinary catheterization (both male and female rats), animal surgery.  Molecular biology: RNA isolation, PCR, Northern Blot analyses, restriction enzyme digest analyses.  Scientific writing: grant writing, paper writing, able to bring science to a level comprehensible by non-scientists.  Teaching: Graduate Level Biology, Pharmacology, High School Biology, Chemistry, Environmental Sciences, IB, Honors, College Preparatory and Basic levels. Curriculum writing, laboratory design, student and club advisory, IB, STEM, use of technology in the classroom
  • 4. pg. 4 Judith Luber-Narod, Ph. D. Publications 1. J. Luber-Narod, B. Smith, W. Grant, J. M. Jimeno, L. López-Lázaro, and G. T. Faircloth.(2001). Evaluation of the use of in vitro methodologies as a tool for screening new compounds for in vivo toxicity potential. Tox. In Vitro 15:571-577. 2. Mitra, S. P., Carraway, R. E., Blute, R., Jr., and Luber-Narod, J. (1999). Agonist induced conformation alteration of neurotensin receptor and the mechanism behind Na+ inhibition of 125I-NT binding. J. Receptor Signal Transduction Res., 19:995-1021. 3. Mitra, S., Dagher, A., Kage, R., Dagher, R. K. and Luber-Narod, J. (1999). Experimental Autoimmune Cystitis: Further characterization and serum antibodies Urol. Res. 27:351-356. 4. Luber-Narod, J., Austin-Ritchie, T., Hollins, C., III, Menon, M., Maholtra, R., Baker, S. and R.E. Carraway. (1997). Role of substance P in several models of bladder inflammation. Urol. Res. 25:395-399. 5. Luber-Narod, J., Austin-Ritchie, T., Banner, B., Hollins, C., Price, H., and Menon, M. (1996). Experimental Inflammatory Cystitis in the Lewis Rat a potential animal model for Interstitial cystitis. Urol. Res. 24:367-373. 6. Laxmanan, S., Bala, R.S., Malhotra, R.K., Miller-Graziano, C., Menon, M. and Luber-Narod, J. (1996). Estrogen induced Autoimmune Prostatitis in the Rat. J. Urol. 156:1838-1840. 7. Scheid, C. R., Koul, H., Hill, W. A., Luber-Narod, J., Jonassen, J., Honeyman, T., Kennington, L., Kholi, R., Hodapp, J., Ayvazian, P., and Menon, M. (1996). Oxalate Toxicity in LLC-PK1 cells, a line of renal epithelial cells. J.Urol. 155:1112-1116.. 8. Scheid, C.R., Koul, H., Kennington, L., Hill, W.A., Luber-Narod, J., Jonassen, J., Honeyman, T., and Menon, M. (1996). Oxalate-induced Damage to Renal Tubular Cells. Scanning Microscopy International 9:1097-1107. 9. Scheid, C. Koul, H., Hill, W.A., Luber-Narod, J. Kennington, L., Honeyman, T., Jonassen, J. and Menon, M. (1996). Oxalate toxicity in LLC-PK1 cells: role of free radicals. Kidney International, 49:413-419. 10. Luber-Narod, J. Kage, R., and S.E. Leeman. (1994). Substance P enhances the secretion of TNF alpha from neuroglial cells stimulated with LPS. J. Immunol. 152:819-824. 11. Ferris, C.F., Delville, Y., Grzonka, Z., Luber-Narod, J., and Insell, T.R. (1993) An iodinated Vasopressin (V1) antagonist blocks flank marking and selectively labels neural binding sites in Golden Hamsters. Physiology and Behavior 54:737-747. 12. Boyd, N.D., MacDonald, S., Kage, R., Luber-Narod, J. and Leeman, S.E. (1991). Substance P receptor: Characterization and G protein interactions. In Substance P and Related Peptides : Cellular and Molecular Physiology., S.E. Leeman, J.E. Krause, and F. Lembeck, ed., N.Y. Academy of Sciences, 1991. 13. Oblas, B., Boyd, N.D., Luber-Narod, J. Reyes, V.E., and S.E. Leeman. (1990). Isolation and identification of a polypeptide in the HSP family that binds substance P. Biochem. Biophys. Res. Comm. 166:978-983. 14. Luber-Narod, J., Boyd, N.D. and Leeman, Susan. (1990). Guanine nucleotides decrease the affinity of substance P binding. European J. Pharmacol., Mol. Pharmacol Section, 188:1-7. 15. Rogers, J., Luber-Narod, J., Mufson, E.J., Styren, S. D., and Civin, W. H. (1988) Presence of immune system markers in human brain and their potential role as a primary pathogenetic mechanism Alzheimer's Disease. In: Molecular Biology of Alzheimer's Disease, Caleb E. Finch and Peter Davies, ed. Cold Spring Harbor Laboratory Press. 16. Rogers, J., and J. Luber-Narod. (1988). Immune actions in the central nervous system: a brief review with special emphasis on Alzheimer's Disease. Drug Develop. Res. 15:227-235. 17. 3. Rogers, J., Luber-Narod, J., Styren, S. D., and W.H. Civin. (1988). Expression of immune system associated antigens by cells of the human central nervous system: relationship to the pathology of Alzheimer's Disease. Neurobiology of Aging, 9:339-349. 18. Luber-Narod, J. and Rogers, J. (1988). Immune system associated antigens expressed by cells of the human central nervous system. Neuroscience Letters, 94:17-22. 19. Potter, L.T., Flynn, D.D., Hanchett, H.E., Kalinoski, D.L., Luber-Narod, J., and Mash, D.C. (1984).Independent M1 and M2 receptors: Ligands, Autoradiography, and Functions.In: Trends in Pharmacological Sciences, Suppl.: 22-32.
  • 5. pg. 5 Judith Luber-Narod, Ph. D. Abstracts 1. Judith Luber-Narod, Ph. D., Brockton High School, Charlie Wheeler, Lauren Chai, Massachusetts Institute of Technology (MIT), Phillipp J. Mehner, Majid Bigdeli Karimi, Northeastern University (NEU), Martin Culpepper, Ph. D., MIT, and Carol Livermore, Ph. D., NEU. Engineering of a Transplantable Liver based on Liver Biology and Origami-folded Materials. (2015), Proceedings of the ERN, AAAS Annual Meeting, February, 2015. 2. Judy Luber-Narod, Jose Maria Fernandez Sousa-Faro, Carmen Cuevas, Pablo Aviles, Glynn T. Faircloth. (2005). In vitro toxicology studies of Lamellarins M, D and K and 4 synthetic analogs. Proceedings AACR, 2005, abstract #639. 3. Susana G. Gómez, Judith Luber-Narod, Glynn T. Faircloth, Juan A. Bueren and Beatriz Albella. (2002). In vitro toxicity of kahalalide f, a new antineoplastic drug. Toxicology In Vitro, Proceedings 2nd international joint meeting: in vitro models and toxicity mechanism. 4. Luber-Narod, J, Ubezio, P., D’Incalci, Maurizio, Grant, W., Smith B. and GT Faircloth.. The in vitro toxicity of ET-743 is antagonized by co-administration with paclitaxel., Toxicology In Vitro, Proceedings 2nd international joint meeting: in vitro models and toxicity mechanism., p. 44. 5. Luber-Narod, J., Smith, B., Grant, W., Jimeno, J., López-Lázaro, L., and GT. Faircloth (2001). In vitro safety toxicology of Ecteinascidin 743, a marine natural product with chemotherapeutic potential against solid tumors, Proceedings AACR 2001, p212. 6. Luber-Narod, J., Smith, B., Grant, W., Jimeno, J., López-Lázaro, L., and GT. Faircloth (2001). In vitro safety profile of Aplidine, a marine natural product with chemotherapeutic potential., Proceedings AACR 2001, p70. 7. Ady-Vago, N., L López-Lázaro, S Faivre, C Lacroix, J Luber-Narod, D. Adams, L Paz-Ares, C Chiabrando, C Guzman, N Celli, GT Faircloth, JP Armand, JM Fernandez-Sousa, JM Jimeno,,and E. Raymond. (2000). L- carnitine as a protector against aplidin induced skeletal muscle toxicity. Proceedings AACR 2001, p. 544 8. Luber-Narod, J, Smith, B, Grant, W, Jimeno, J, López-Lázaro, L, Scotto, K, Shtil, A. and Faircloth G.T. (2000). In vitro safety toxicology of Kahalalide F, a marine natural product with chemotherapeutic potential against solid tumors. EORTC-AACR Clin. Canc. Res. 9. Luber-Narod, J. and Delville, Y. (1999). Ovariectomy decreases urinary frequency in an animal model. NE section, American Urological Association Annual Meeting. 10. 24. Luber-Narod, J. and Delville, Y. (1999). Ovariectomy decreases urinary frequency in an animal model. Soc. Neurosci. Abst. 11. Luber-Narod, J. (1999). Glycosaminoglycan changes in the urine of rats with experimental autoimmune cystitis (EAC). J. Autoimmunity, Suppl. 1999, p.51. 12. Luber-Narod, J. (1999). J. Experimental autoimmune cystitis (EAC): Role of autoantibodies. J. Autoimmunity, Suppl. 1999, p.106. 13. Luber-Narod, J. (1998). 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Luber-Narod, Austin-Ritchie, T., Price, H., Dagher, R., Menon, M. and R.E. Carraway. (1995). Role of neuropeptides in the xylene induced model of bladder inflammation. NIDDK conference on Interstitial Cystitis. 19. Scheid, C. Koul, H., Kennington, L., Hill, A., Luber-Narod, J., and Menon, M. (1994) Oxalate induced damage in renal epithelial cells, a possible factor in kidney stone disease. FASEB abstracts 8:408. 20. Luber-Narod, J. Kage, R. and Leeman, S.E. (1994). Substance P stimulates the secretion of tumor necrosis factor from rat brain glia in culture. IBRO abst. 21. MacDonald, S.G., Luber-Narod, J. and Boyd, N.D. (1990). Crosslinking of the substance P receptor to a 43 kDa polypeptide: a potential G protein alpha subunit. Soc. Neurosci. Abst. 14:515. 22. Luber-Narod, J., Boyd, N.D., Oblas, B., and S.E. Leeman (1987). A GTP-insensitive component of high affinity substance P binding to receptors from rat salivary gland. Soc. Neurosci. Abst. 13:412. 23. 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  • 6. pg. 6 Judith Luber-Narod, Ph. D. 24. Rogers, J. , Styren, S. D., and Luber-Narod, J. (1987). Expression of immune system associated antigens by cells of the human central nervous system . Soc. Neurosci. Abst.13:1328. 25. Rogers, J., Luber-Narod, J., and Styren, S.D. (1987). Expression of immune system markers in human brain and its potential pathogenetic significance in Alzheimer's Disease. Satellite symposium on Autoimmunity and Alzheimer's Disease, Society for Neurosciences. 26. Rogers. J., Singer, R.H., Luber-Narod, J., and Bassell, G. (1986). Neurovirologic and neuroimmunologic considerations in Alzheimer's Disease. Soc. Neurosci. Abst. 12:944. 27. Luber-Narod, J. , Flynn, D.D., and Potter, L.T. (1984). Binding of selective agonists and antagonists to two interconvertible states of M1 muscarine receptors. Soc. Neurosci. Abst. 10:568. 28. Potter, L.T. and Luber-Narod, J. (1983). Distribution and function of M1 and M2 muscarine receptors in different tissues. Soc. Neurosci. Abst. 9:582. 29. Luber-Narod, J. and Potter, L.T. (1983) Selectivity of drugs for M1 and M2 muscarine receptors. Soc. Neurosci. Abst. 9:582. 30. Potter, L.T., Luber-Narod, J., and Wohlberg, C.J. (1982). EDTA amplifies agonist- and antagonist-binding phenomena, and helps resolve different types of receptors. Soc. Neurosci. Abst. 8:339. 31. Flynn, D.D., Kalinoski, D.L., Luber-Narod, J. and Potter, L. T. (1982). Evidence for guanine nucleotide-sensitive regulatory protein with muscarine receptors in membranes and in solution. Soc. Neurosci. Abst. 8:339. 32. Luber-Narod, J. and Potter, L.T. (1982). Two types of muscarine receptors (M1 and M2) in membranes from the rat forebrain and brainstem. Soc. Neurosci. Abst. 8:338. 33. Luber-Narod, J. and Potter, L.T. (1981). Three agonist and two antagonist states of muscarine receptors. Soc. Neurosci. Abst. 7:498.