th
6510 NW 27 St.                  (352) 505-5190
Gainesville, FL 32653           ndungan@gmail.com


Nathanael W. Dungan
Objective:              To pursue a fulfilling full-time position in the biotechnology, pharmaceutical or animal health field.

Education:              Aug 98 – Dec 01             MidAmerica Nazarene University - Olathe, KS
                                                    B.A. General Science Education with Biology Emphasis
                                                           Dean’s List
                                                           Graduated with 3.57 GPA

                        Aug 96 – May 98             Southeast Community College - Beatrice, NE
                                                           Phi Theta Kappa (Honor Society) Member
                                                           Transfer student

Professional            April 2008 - Current - Nanotherapeutics, Inc.
Experience:             Preclinical Manager
                        Responsibilities:
                                         Oversee completion of animal pharmacokinetic (PK) testing and submission of
                                         samples for analysis
                                         Manage animal technicians responsible for daily hands on animal work
                                         Entry and initial analysis of data received from analytical department
                                         Drafting of standard operating procedures, work instructions, forms, study
                                         protocols, IACUC protocols and other documents related to the completion of
                                         animal work
                                         Drafting of monthly progress reports and final reports of work completed
                                         Ordering of supplies needed for completion of animal work

                        June 2006 – April 2008 - St. Charles Pharma
                        Research Associate / Research and Development
                        Responsibilities:
                                         Lab organization and lab supply inventory and ordering
                                         Data entry and analysis using LabView, Microsoft Excel, and GraphPad Prism
                                         Writing of Research Reports and Standard Operating Procedures
                                         Internet research of assays and surgical techniques
                                         Handling of mice and rats including dosing by oral gavage, intraperitoneal (IP)
                                         injections, feeding and weighing, and euthanizing and necropsy
                                         Assistance with rodent pain model testing including the abdominal stretch,
                                         formalin, orofacial operant, and rearing activity models
                                         Construction of mouse rearing apparatus for testing animal activity following acetic
                                         acid IP injection
                                         Development of video scoring system for accurate and efficient data gathering
                                         Upkeep and organization of lab notebook including details and results from all lab
                                         work completed
                                         Measurement and preparation of drug solutions for use during pain model testing

                        December 2002 – May 2006 - Intervet, Inc
                        Lab Technician III / Research and Development
                        Laboratory skills in Virology and Bacteriology:
                        Responsibilities:
                        Virology:
                                         Haemoagglutination (HA) and Haemoagglutination Inhibition (HI) Assays
                                         Serum Neutralization (SN) Assay
                                         Mammalian cell culture
                                         Virus growth and titrations by TCDI50 and immunoflourescent antibody (IFA)
                                         staining
                                         Virus passage and titration in SPF chicken embryos
                                         Preparation of bulk virus in cell culture systems
Immuno assays including ELISA
                             Preparation of serum and colostrum for testing
                             BCA protein assay

             Bacteriology:
                             Mass cultivation and titration of bacteria using various media
                             Bacterial fermentations in small and large fermentor units (12L and 50L)
                             Fermentation fluid concentration techniques including hollow fiber
                             Endotoxin estimation testing of gram negative organisms
                             Production of bacterial autogenous vaccines
                             Bacterial inactivation kinetics

             Organisms directly involved in research:

             Virus:
                             Swine Influenza Virus
                             Bovine Rotavirus
                             Bovine Coronavirus

             Bacteria:
                             Pasteurella multocida
                             Mannheimia haemolytica
                             Haemophilus somnus
                             Actinobacillus pleuropneumonia
                             Haemophilus parasuis
                             Erysipelothrix rhusiopathiae

             General Skills include:
                              Aseptic techniques
                              Media preparation (liquid and plate)
                              Purity testing techniques
                              Production documentation similar to GMP
                              Laboratory documentation similar to GLP
                              Operation of autoclaves, fume hoods, spectrophotometer, pH probes, microscopes
                              (inverted and FA), centrifuges
                              Large animal handling including bleeding, vaccination, challenge, clinical
                              observations and necropsy

             January 2002 - December 2002 - Olathe, KS School District
             Substitute Teacher
             Responsibilities:
                              Complete daily lesson plan as outlined by the absent teacher during single day
                              teaching assignments
                              Prepare daily lesson plans, teach lessons, prepare and grade homework and tests
                              as part of a semester long substitute assignment
                              Prepare for and participate in parent / teacher conferences
                              Prepare and submit final semester grades

Additional                   Proficient in computer technology including Microsoft Office Applications
Skills:                      Excellent verbal and written skills
                             Strong organization, tabulation, analysis and presentation of scientific data

Resume

  • 1.
    th 6510 NW 27St. (352) 505-5190 Gainesville, FL 32653 ndungan@gmail.com Nathanael W. Dungan Objective: To pursue a fulfilling full-time position in the biotechnology, pharmaceutical or animal health field. Education: Aug 98 – Dec 01 MidAmerica Nazarene University - Olathe, KS B.A. General Science Education with Biology Emphasis Dean’s List Graduated with 3.57 GPA Aug 96 – May 98 Southeast Community College - Beatrice, NE Phi Theta Kappa (Honor Society) Member Transfer student Professional April 2008 - Current - Nanotherapeutics, Inc. Experience: Preclinical Manager Responsibilities: Oversee completion of animal pharmacokinetic (PK) testing and submission of samples for analysis Manage animal technicians responsible for daily hands on animal work Entry and initial analysis of data received from analytical department Drafting of standard operating procedures, work instructions, forms, study protocols, IACUC protocols and other documents related to the completion of animal work Drafting of monthly progress reports and final reports of work completed Ordering of supplies needed for completion of animal work June 2006 – April 2008 - St. Charles Pharma Research Associate / Research and Development Responsibilities: Lab organization and lab supply inventory and ordering Data entry and analysis using LabView, Microsoft Excel, and GraphPad Prism Writing of Research Reports and Standard Operating Procedures Internet research of assays and surgical techniques Handling of mice and rats including dosing by oral gavage, intraperitoneal (IP) injections, feeding and weighing, and euthanizing and necropsy Assistance with rodent pain model testing including the abdominal stretch, formalin, orofacial operant, and rearing activity models Construction of mouse rearing apparatus for testing animal activity following acetic acid IP injection Development of video scoring system for accurate and efficient data gathering Upkeep and organization of lab notebook including details and results from all lab work completed Measurement and preparation of drug solutions for use during pain model testing December 2002 – May 2006 - Intervet, Inc Lab Technician III / Research and Development Laboratory skills in Virology and Bacteriology: Responsibilities: Virology: Haemoagglutination (HA) and Haemoagglutination Inhibition (HI) Assays Serum Neutralization (SN) Assay Mammalian cell culture Virus growth and titrations by TCDI50 and immunoflourescent antibody (IFA) staining Virus passage and titration in SPF chicken embryos Preparation of bulk virus in cell culture systems
  • 2.
    Immuno assays includingELISA Preparation of serum and colostrum for testing BCA protein assay Bacteriology: Mass cultivation and titration of bacteria using various media Bacterial fermentations in small and large fermentor units (12L and 50L) Fermentation fluid concentration techniques including hollow fiber Endotoxin estimation testing of gram negative organisms Production of bacterial autogenous vaccines Bacterial inactivation kinetics Organisms directly involved in research: Virus: Swine Influenza Virus Bovine Rotavirus Bovine Coronavirus Bacteria: Pasteurella multocida Mannheimia haemolytica Haemophilus somnus Actinobacillus pleuropneumonia Haemophilus parasuis Erysipelothrix rhusiopathiae General Skills include: Aseptic techniques Media preparation (liquid and plate) Purity testing techniques Production documentation similar to GMP Laboratory documentation similar to GLP Operation of autoclaves, fume hoods, spectrophotometer, pH probes, microscopes (inverted and FA), centrifuges Large animal handling including bleeding, vaccination, challenge, clinical observations and necropsy January 2002 - December 2002 - Olathe, KS School District Substitute Teacher Responsibilities: Complete daily lesson plan as outlined by the absent teacher during single day teaching assignments Prepare daily lesson plans, teach lessons, prepare and grade homework and tests as part of a semester long substitute assignment Prepare for and participate in parent / teacher conferences Prepare and submit final semester grades Additional Proficient in computer technology including Microsoft Office Applications Skills: Excellent verbal and written skills Strong organization, tabulation, analysis and presentation of scientific data