This study analyzed 100 prescriptions from hospital and community pharmacies in Pakistan to assess polypharmacy, antibiotic and supplement use. The key findings were: 1) 40% of prescriptions involved polypharmacy (≥5 medications) and 19% included nutritional supplements. 2) The most common drug interactions were between NSAIDs, antihypertensives, and antibiotics. 3) Elderly patients were most likely to be on polypharmacy regimens and use supplements. The average cost per prescription was 174.70 Pakistani rupees.