Abstract of the Invited talk presented by Prof Satya P. Singh, in One Day International Virtual Conference on ‘Advances in Plant Sciences’ Held by: Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, India ABSTRACT Attributes of Plant Growth Promoting Rhizobacteria in biocontrol and combating salinity stress

1. Prof. Satya P. Singh, PhD
UGC-BSR Faculty Fellow (Professor-Emeritus)
UGC-CAS Department of Biosciences
Saurashtra University
RAJKOT-360 005. Gujarat, INDIA
Head, Department of Biosciences, Saurashtra University (2003-2020)
Coordinator, UGC-CAS Program, Saurashtra University (2013-2018)
Coordinator, DST-FIST Program (2007-2012)
Coordinator/PI, DBT-Multi Institutional Project (2007-2012)
PI, MoES-Multi Institutional Net Working Project (2014-2018)
Coordinator, M.Sc. Biotechnology, Saurashtra University (2004-2017)
Coordinator, UGC DSA-Phase-3 and UGC COCIST (2003-2006)
Email: satyapsingh@yahoo.co satyapsingh125@gmail.com
spsingh@sauuni.ac.in
SU Website URL: saurashtrauniversity.edu/university/academic-
departments/department-of-biosciences/staff/dr-satya-prakash-singh/
ResearchGate: https://www.researchgate.net/profile/Satya_Singh5
GoogleScholar: https://scholar.google.com/citations?hl=en&user=jiAzOcg
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UGC: https://vidwan.inflibnet.ac.in//profile/68903/Njg5MDM%3D
Abstract of the Invited talk presented by Prof Satya P. Singh, in
One Day International Virtual Conference on ‘Advances in Plant
Sciences’
Held by:
Department of Botany, University College of Science, Mohanlal
Sukhadia University, Udaipur, Rajasthan, India

2. ABSTRACT
Attributes of Plant Growth Promoting Rhizobacteria in biocontrol and
combating salinity stress
Satya P. Singh
UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India
Abstract
In this presentation, aspects of the Halophilic/Haloalkaliphilic bacteria, rhizosheric
microbiome, metagenomics and proteomics have been discussed with particular reference to
plant growth and biocontrol under salinity stress. Adaptations to the extremity in
microorganisms are conferred at various levels; cell structure, membrane transport,
metabolism, structural changes in the macromolecules, thermodynamics and information
processing machinery. Based on 16 S rRNA gene sequences, fatty acid profiling, lipid
contents number of novel strains have been identified with the assignment of new genera and
species. The enzymes of the Haloalkaliphilic Bacteria represent certain unique features, such
as catalysis and stability under multitudes of the extremity of salt, pH, temperature, organic
solvents. Salt dependent temperature profile and resistance to chemical denaturation are
unique features of their certain enzymes. Many enzymes have been cloned and expressed in
different hosts analyzing the factors affecting the gene expression.
Salinity adversely affects plant growth by altering physiological and metabolic traits. About
20% of the world’s irrigated land is affected by salinity. Although amendments with gypsum
and CaCl2 have limited success, it adversely affects the agro-ecosystems. Thus, a sustainable
and eco-friendly approach is necessary. Rhozobacteria can alleviate the adverse impact of the
salinity and promote growth and protection against soil-borne pathogens through various
strategies; efflux and ion Transport, accumulation of compatible solutes, induction of gene
expression, synthesis of exopolysaccharides, activation of antioxidant machinery and
phytohormones. PGPR isolated from various halophytes of Costal Gujarat reflected variable
and diverse PGPR traits.
Rhizosphere microbiome is highly important in the plant ecosystems. However, only limited
knowledge exits on this aspect. Nevertheless, with the advancement of the NGS Techniques,
the establishment of the correlation between the community ecology and physiology in the
rhizosphere is getting impetus. Identification of the specific chemical and microbial markers
on how plants recruit and stimulate beneficial microorganisms would be significant step in
improving the crop production and protection under stress conditions. There exist a set of
core microorganisms responsible to effectively protect plants from pathogens and stress.
However, in the direction of designing microbiome, it’s important to address on the core
microbiome and minimal microbiome. Although, it is not certain what would be the minimal
microbiome? This question is relevant to avoid antagonism and functional redundancy in the
population of a given ecosystem.

3. Key Words: PGPR, Salt Tolerance, Rhozospheric Microbiome, Minimal Microbiome,
Conserved Microbiome, Halophilic/Haloalkaliphilic bacteria, Salt dependent-chemical
denaturation
References:
➢ Dwivedi, Purna, Sharma, A. K. and Singh, S.P. 2021. Biocatalysis and Agricultural Biotechnology, In
Press.
➢ Kikani, B.A. and Singh, S.P. 2021. Critical Reviews in Biotechnology, In Press, 2021
➢ Rathore, D. R., Sheikh, M., Gohel G.D, and Singh, S.P. 2021. Current Microbiology, 78: 1377-1387
➢ Bhatt, H.B., Begum, M.A., Chintalapati, S., Chintalapati, V.R. and Singh, S.P. 2017. International
Journal of Systematic & Evolutionary Microbiology (IJSEM), 67(11):4435-4442
➢ Gohel, S. D. and Singh S.P. 2018. Geomicrobiology Journal, 35:9, 775-789
➢ Nowlan B., Dodia, M.S., Singh, S.P and Patel, B. K. C. 2006. Int J Syst Evol. Microbiol 56:1073-1077
➢ Dodia M. S., Rawal C. M., Bhimani H. G., Joshi R. H., Khare, S. K. and Singh, S. P. 2008. Journal of
Industrial Microbiology & Biotechnology 35(2):121-132
➢ Raval, V. Rawal, C.M., Pillai, S. and Singh S.P. 2014. Process Biochemistry 49 (6): 955-962
➢ Dodia M. S., Rawal C. M., Bhimani H. G., Joshi R. H., Khare, S. K. and Singh, S. P. 2008. Journal of
Industrial Microbiology & Biotechnology 35(2):121-132
➢ Egamberdieva1 Dilfuza et al., Frontiers in Microbiology, published: 18 December 2019 doi:
10.3389/fmicb.2019.02791
➢ Rodrigo Mendes, Paolina Garbeva, Jos M. Raaijmakers, FEMS Microbiology Reviews, 37(5): 634–
663, 2013, https://doi.org/10.1111/1574-6976.12028
➢ Arora et al., Journal of Advanced Research 26 (2020) 69–82
➢ Timm, et al., 2018, mSystems (ASM)
➢ Tracanna, mSystems 6:e01116-20, 2021,https://doi.org/10.1128/mSystems.01116-20
➢ Nilde Antonella Di Benedetto et al., MDPI, Microorganisms 2019, 7, 541;
doi:10.3390/microorganisms7110541
➢ Bhatt, H.B. and Singh S.P. 2020, 11:1-16, https://doi.org/10.3389/fmicb.2020.00941
➢ Sharma, A.K. Kikani, B.A. and Singh S.P. 2020, International Journal of Biological Macromolecules
(IJBIOMAC), 153:680-696, DOI: 10.1016/j.ijbiomac.2020.03.006
➢ Sharma, A.K. Kikani, B.A. and Singh S.P. 2020, Geomicrobiology Journal, DOI:
10.1080/01490451.2020.1860165
➢ Raiyani, Nirali and Singh S.P. 2020, Genomics, 112:4361- 4369
https://doi.org/10.1016/j.ygeno.2020.07.024