This document contains the educational and professional background of Dr. Murlidhar S. Jadhav. It summarizes his educational qualifications including a B.Sc. in Agriculture, M.Sc. in Agricultural Biotechnology, and Ph.D. in Plant Biotechnology. It also outlines his research experience during his M.Sc. and Ph.D. focusing on developing insect-resistant transgenic cotton plants. His professional experience includes teaching agricultural biotechnology and conducting research in areas such as plant transformation, molecular markers, and molecular characterization of plants.

1. Year Degree Board/University/Institute
Class and %
marks
2011-2015 Ph.D. ( Plant Biotechnology)
Tamil Nadu Agricultural
University (TNAU), Coimbatore
(T.N)
Ist
with 83.3
2009
M.Sc. (Agriculture)
Agricultural Biotechnology
C.S.K. Himachal Pradesh Krishi
Vishvavidyalaya, Palampur,
(H.P)
Ist
with 75.00
2007 B.Sc. (Agriculture)
Marathwada Agricultural
University (MAU), Parbhani
(M.S)
Ist
with 82.6
2003
H.S.C. (10+2) MSBSHE, Pune (M.S) Ist
with 71.83
2000 S.S.C. MSBSHE, Pune (M.S) Ist
with 65.86
2010
ICAR NET (Basic Plant
sciences)
ASRB, ICAR, New Delhi
Qualified
Title of the Ph.D. Thesis:
“Generation and Analysis of Insect Resistant Transgenic Cotton Plants Expressing cry2AX1
Gene”
Research undertaken during Ph.D.
During my doctoral research I have gained expertise in Cotton and Tobacco tissue culture and its
genetic transformation using Agrobacterium method. Also developed insect resistant cry2AX1
transgenic cotton plants through somatic embryogenesis, in the Coker 310 background and did its
molecular characterization, which included PCR, RT-PCR, Southern blot hybridization analysis,
qualitative and quantitative ELISA and insect bioassay. Besides this I gained experience in transgenic
elite event characterization in advanced T3 generation progeny for stability of gene expression using
PCR, southern blot analysis, ELISA and Helicoverpa armigera insect bioassay to assess the bio-
efficacy Bt protein. Further I worked on development of new plant expression constructs for
enhanced expression of cry2AX1 (Bt) gene in plant and their expression validation in tobacco.
Besides, I have experience in various bioinformatics tools viz. nucleotide sequence assembly tools,
sequence alignments tools, construction of phylogenetic trees.
Academic research experience:

2. Title of M.Sc. Thesis:
“Identification of sex-specific marker(s) in sea buckthorn Hippophae rhamnoides L.”
Research undertaken during M.Sc.:
1. Identification of DNA markers linked to sex in Hippophae rhamnoides (dioecious medicinal
plant)
2. Molecular characterization of Hippophae rhamnoides populations of north western Himalayan
region of India using PCR-based markers RAPD, SSR, ISSR, MADS-box gene specific
marker and ITS region Markers
3.
Working on isolation and characterization of fiber specific promoters and genes (CesA1 and cesA2)
from cotton, to improve the cotton fiber strength, at ICAR-CICR, Nagpur (Maharashtra). (five
months)
Plant transformation, gene cloning, Molecular markers, MAS breeding, Molecular phlyogenetics,
plant viruses and plant tissue culture.
Transgenic development, Molecular and biochemical characterization of transgenic plants, Insect
bioassay, Gender specific marker development, sex specific marker development, Molecular
characterization and phlyogenetic analysis of plants, Gene isolation, cloning and validation,
Development of plant expression constructs, Molecular characterization of viruses and infectious
clone construction and basic molecular biology techniques,
 Teaching B.Sc. Agricultural Biotechnology students at Aditya college of Agricultural Biotechnology,
Beed, Maharashtra.( one and half year)
Area of research:
Expertise
Teaching Experience:
Current/ Post-doctoral research experience:
experience:

3.  Courses Taught : Molecular Biology, Biochemistry, Plant Tissue culture, Recombinant DNA
technology, Enzyme biotechnology
1. Awarded ICAR Senior Research Fellowship (ICAR-SRF) for Doctoral programme in
Biotechnology (2011-2014) by ICAR, New Delhi.
2. Awarded merit fellowship from Department of Biotechnology (DBT) Govt. of India during
M. Sc. (Agril. Biotechnology) Programme (2007-2009).
3. National Finalist of “Anveshan” Student Research Convention- 2008, With First rank in North
Zone Final organized by Association of Indian Universities, New Delhi and Panjab
University, Chandigarh from Feb. 13-15, 2008.
4. First prize for poster presentation in BIOCONCORRENZA-2015 A National level technical
symposium organized by CPMB&B, Tamil Nadu Agricultural University, Coimbatore (T.N)
1. Indian society of Plant Breeders, Coimbatore, INDIA
2. Indian Society of Genetics and Biotechnology, Agra, INDIA
1. M. S. Jadhav, A.R. Sakthi, M. Rajeshwari, N. Balakrishnan, D. Sudhakar and V. Udayasuriyan
(2015) Construction of plant transformation vectors harboring synthetic cry2AX1 gene and its
expression in tobacco (Nicotiana tabacum). Indian Res. J. Genet. & Biotech. 7(2): 160 – 168.
2. M. S. Jadhav, N. Balakrishnan, D. Sudhakar and V. Udayasuriyan (2015). Genetic
Transformation of Cotton for Bollworm Helicoverpa armigera (Hubner) Resistance Using
Chimeric cry2AX1 Gene. Indian Research Journal of Science and Technology. 8(17): 1-7
3. K. Lovejot., M. S. Jadhav, N. Senthil, N. Bharthi,V. G. Malathi and P. Nagarajan (2015)
Molecular cloning and characterization of Yellow Mosaic Virus from mungbean from northern
region of Tamil Nadu indicates association of Mungbean Yellow Mosaic India Virus DNA A with
a recombinant DNA B. Journal of Mycology and Plant Pathology Vol. 45 no. (2) 173-181
4. Jadhav M. S*. (2015). An update on important functionally characterized genes / QTLs of
agronomic importance in crop plants. Indian Res. J. Genet. & Biotech. 7(1) : 44 –49
Fellowships/ awards:
Life Membership in Professional Bodies / Societies
Publications (Research Articles):

4. 5. Jadhav M. S., Bhawna Verma, Naresh Kumar, R. K. Chahota, V. Singh, R. Rathour, Sunil K.
Singh, S. K. Sharma and T. R. Sharma (2013). Analysis of molecular genetic diversity and
population structure in sea buckthorn (Hippophae spp L.) from north-western Himalayan region of
India. Journal of Medicinal Plant Research. 7(43):3183-3196.
6. Jadhav M. S.* and Sharma T.R. (2014). Identification of gender Specific DNA Markers in Sea
buckthorn (Hippophae rhamnoides L.) Indian Res. J. Genet. & Biotech. 6(3) : 464-46
7. Jadhav M. S.* and Sharma T. R. (2014). Molecular authentication of Hippophae species and
subspecies and their phylogenetic analysis based on nuclear ITS sequences from north western
Himalayan region of India. Research Journal of Biotechnology Vol. 9(10): 62-68
8.
9.
1. 1. Jadhav M. S., A. B. Rajurkar and Seema Rana (2015).Virus induced gene silencing in plants.
AGROBIOS NEWSLETTER Vol. XIII (10) page no. 20-21 ISSN:972-7027
2. Jadhav M. S. and Manape Tushar K. (2015). Advanced tools in plant genome editing
(Engineering). AGROBIOS NEWSLETTER Vol. XIII (10) page no. 18 ISSN:972-7027
3. 3. Manape Tushar K and Jadhav M. S. (2015). Selectable Marker for plastid transformation.
AGROBIOS NEWSLETTER Vol. XIII (11) page no. 12 ISSN:972-7027
4. 4. Manape Tushar K and Jadhav M. S. (2015). Algae as platform for oral recombinant vaccine
production. AGROBIOS NEW SLETTER Vol. XIII (11) page no. 24 ISSN:972-7027
NCBI Gene Bank Sequence Depositions:
1. Lovejot, K., Jadhav, M.S., Malathi,V.G., Natesan, S. and Pothi, N. (2014) Molecular
characterization of yellow mosaic virus from mungbean from northern region of Tamil Nadu
indicates association of mungbean yellow mosaic India virus DNA A with a recombinant DNA
B. NCBI accession No.# KP319016
2. Lovejot, K., Jadhav, M.S., Malathi,V.G., Natesan, S. and Pothi, N. (2014) Molecular
characterization of yellow mosaic virus from mungbean from northern region of Tamil Nadu
indicates association of mungbean yellow mosaic India virus DNA A with a recombinant DNA
B. NCBI accession No.# KP313758
3. Lovejot, K., Jadhav, M.S., Malathi,V.G., Natesan, S. and Pothi, N. (2014) Molecular
characterization of yellow mosaic virus from mungbean from northern region of Tamil Nadu
indicates association of mungbean yellow mosaic India virus DNA A with a recombinant DNA
B. NCBI accession No.# KP319017
Popular Articles
Conferences & Workshops:

5. 1. Prabhakar, C. S., Rana, L. and Jadhav, M. S. 2008. DNA barcoding of Indian fruit flies
(Tephritidae: Diptera) for National biosecurity. “Anveshan” Student Research Convention-
2008, organized by Association of Indian Universities, New Delhi and Panjab University,
Chandigarh from Feb. 13-15, 2008
2. Jadhav M.S., R. Rathour, and T. R. Sharma (2009). In Souvenir and abstracts: National
Symposium on “Recent Global Developments in the Management of Plant Genetic
Resources” 17-18, December, 2009 at NBPGR, New Delhi (Abstr. C-121 pp 212-213
3. Attended short term training course on “Application of Bioinformatics’ Tools in Genome
Analysis at Bioinformatics Centre, Department of Agricultural Biotechnology, CSK HP
Agriculture University, and Palampur.
4. Attended short term training course on “Computers in Computational biology at
Bioinformatics Centre, Department of Agricultural Biotechnology, CSK HP Agriculture
University, Palampur.
1. Dr. V. Udayasuriyan, PhD
Professor,
Centre for Plant Molecular Biology and
Biotechnology, Tamil Nadu
Agricultural University, Coimbatore-
641 003 (Tamil Nadu) India,
E-mail: udayvar@yahoo.com
Phone: +919442007321
2. Dr. T.R. Sharma, PhD
Professor and Head
Department of Plant Biotechnology,
CSK HPKV, Palampur-176062
(Himachal Preadesh), India
E-mail: sharma88@yahoo.com
Phone:+919418057601
Declaration
I hereby declare by stating that the above information is true and correct to the best of my knowledge
and belief.
Place: Nagpur, India. Yours sincerely
Date: 16 th
December, 2015
(Dr. Murlidhar S. Jadhav)
References:

6. 1. Prabhakar, C. S., Rana, L. and Jadhav, M. S. 2008. DNA barcoding of Indian fruit flies
(Tephritidae: Diptera) for National biosecurity. “Anveshan” Student Research Convention-
2008, organized by Association of Indian Universities, New Delhi and Panjab University,
Chandigarh from Feb. 13-15, 2008
2. Jadhav M.S., R. Rathour, and T. R. Sharma (2009). In Souvenir and abstracts: National
Symposium on “Recent Global Developments in the Management of Plant Genetic
Resources” 17-18, December, 2009 at NBPGR, New Delhi (Abstr. C-121 pp 212-213
3. Attended short term training course on “Application of Bioinformatics’ Tools in Genome
Analysis at Bioinformatics Centre, Department of Agricultural Biotechnology, CSK HP
Agriculture University, and Palampur.
4. Attended short term training course on “Computers in Computational biology at
Bioinformatics Centre, Department of Agricultural Biotechnology, CSK HP Agriculture
University, Palampur.
1. Dr. V. Udayasuriyan, PhD
Professor,
Centre for Plant Molecular Biology and
Biotechnology, Tamil Nadu
Agricultural University, Coimbatore-
641 003 (Tamil Nadu) India,
E-mail: udayvar@yahoo.com
Phone: +919442007321
2. Dr. T.R. Sharma, PhD
Professor and Head
Department of Plant Biotechnology,
CSK HPKV, Palampur-176062
(Himachal Preadesh), India
E-mail: sharma88@yahoo.com
Phone:+919418057601
Declaration
I hereby declare by stating that the above information is true and correct to the best of my knowledge
and belief.
Place: Nagpur, India. Yours sincerely
Date: 16 th
December, 2015
(Dr. Murlidhar S. Jadhav)
References: